Introduction There is a downward trend of stroke-related mortality in the USA.By reviewing all published articles on stroke mortality in China,we analysed its trend and possible factors that have influenced the trend....Introduction There is a downward trend of stroke-related mortality in the USA.By reviewing all published articles on stroke mortality in China,we analysed its trend and possible factors that have influenced the trend.Methods Both English and Chinese literatures were searched on the mortality of stroke or cerebrovascular diseases in China.Potential papers related to this topic were identified from PubMed,Medline,Embase,Cochrane Library,Wanfang Database,SINOMED and China National Knowledge Infrastructure databases.Results Comparing the results from the most recent population-based epidemiological survey and databank from the national Center for Disease Control and Prevention,the age-adjusted stroke mortality rate has shown a downward trend among both urban and rural population in the past 30 years in China.Comparing with 30 years ago,the rate of stroke mortality has decreased by more than 31%in urban/suburban population and 11%in rural population.In men,the age-adjusted stroke mortality rate decreased by 18.9%and in women by 24.9%between 1994 and 2013.Factors that may have contributed to the trend of decreased stroke mortality rate include(1)improved healthcare coverage and healthcare environment;(2)improved treatment options and medical technology;(3)support by government to educate the public on stroke and stroke prevention;and(4)improved public knowledge on stroke.Conclusions The age-adjusted stroke mortality rate in China has shown a downward trend among both urban and rural population in the past 30 years.The major influencing factors that helped in reducing stroke mortality in China included improved healthcare coverage,healthcare environment,the updated treatment options and modern medical technology.展开更多
The pandemic of coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has posed serious threats to global health and economy,thus calling for the development of safe an...The pandemic of coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has posed serious threats to global health and economy,thus calling for the development of safe and effective vaccines.The receptorbinding domain(RBD)in the spike protein of SARS-CoV-2 is responsible for its binding to angiotensin-converting enzyme 2(ACE2)receptor.It contains multiple dominant neutralizing epitopes and serves as an important antigen for the development of COVID-19 vaccines.Here,we showed that immunization of mice with a candidate subunit vaccine consisting of SARS-CoV-2 RBD and Fc fragment of human IgG,as an immunopotentiator,elicited high titer of RBD-specific antibodies with robust neutralizing activity against both pseudotyped and live SARS-CoV-2 infections.The mouse antisera could also effectively neutralize infection by pseudotyped SARS-CoV-2 with several natural mutations in RBD and the IgG extracted from the mouse antisera could also show neutralization against pseudotyped SARS-CoV and SARS-related coronavirus(SARSr-CoV).Vaccination of human ACE2 transgenic mice with RBD-Fc could effectively protect mice from the SARS-CoV-2 challenge.These results suggest that SARS-CoV-2 RBD-Fc has good potential to be further developed as an effective and broad-spectrum vaccine to prevent infection of the current SARS-CoV-2 and its mutants,as well as future emerging SARSr-CoVs and re-emerging SARS-CoV.展开更多
Co-culture engineering is an emerging approach for microbial biosynthesis of a variety of biochemicals.In this study,E.coli-E.coli co-cultures were developed for heterologous biosynthesis of the natural product naring...Co-culture engineering is an emerging approach for microbial biosynthesis of a variety of biochemicals.In this study,E.coli-E.coli co-cultures were developed for heterologous biosynthesis of the natural product naringenin.The co-cultures were composed of two independent E.coli strains dedicated to functional expression of different portions of the biosynthetic pathway,respectively.The co-culture biosynthesis was optimized by investigating the effect of carbon source,E.coli strain selection,timing of IPTG induction and the inoculation ratio between the co-culture strains.Compared with the monoculture strategy,the utilization of the designed co-cultures significantly improved the naringenin production,largely due to the reduction of metabolic stress,employment of proper hosts for improving pathway enzyme activities,and flexible adjustment of the relative biosynthetic strength between the coculture strains.The findings of this study extend the applicability of co-culture engineering in complex natural product biosynthesis.展开更多
The early Precambrian high-grade metamorphosed basement in the Xi Ulanbulang area, central Inner Mongolia of China, is composed mainly of intermediate granulites and charnockitic gneisses. Both types of the rocks are ...The early Precambrian high-grade metamorphosed basement in the Xi Ulanbulang area, central Inner Mongolia of China, is composed mainly of intermediate granulites and charnockitic gneisses. Both types of the rocks are closely associated spatially and temporally, with a gradual variation between them. In order to understand timing of the high-grade metamorphism, we carried out SHRIMP U-Pb dating of zircons of the rocks. Zircons from the granulites and charnockitic gneisses are similar in structure and age. Zircon cores show magmatic zoning and have ages of 2507-2545 Ma. The ages are interpreted as the forming time of protolith of the granulites and charnockitic gneisses, indicating that a strong magmatism existed at that time in the Yinshan Block. The zircon mantles and rims show homogeneous structures and record a strong granulite facies metamorphism event around 2500 Ma, with a time interval between the metamorphism and magamatism being less than 50 Ma. These suggest that the Western Block was similar to the Eastern Block in tectono-thermal timing at the end of the Neoarchean.展开更多
A new compressibility correlation is introduced in the Langtry＇s local variable-based transition model to investigate the phe- nomenon on double wedge shock/boundary layer interactions. The cmnputational analysis com...A new compressibility correlation is introduced in the Langtry＇s local variable-based transition model to investigate the phe- nomenon on double wedge shock/boundary layer interactions. The cmnputational analysis compared with experimental data has been made to assess the influence of the wall temperature and the leading edge nose radius on a hypersonic double wedge boundary layer. It has been found that the laminar boundary layer separation occurs on the first ramp. Furthermore, the wall temperature and the leading edge nose radius have remarkable influence on the separation characteristics in the kink. Comparison of the calculated pressure coefficient distribution and the boundary layer profile with the experimental data shows that better results can be achieved when using the modified transition model.展开更多
Blast, caused by Magnaporthe oryzae, is one of the most widespread and destructive diseases of rice. Breeding durable resistant cultivars （cvs） can be achieved by pyramiding of various resistance （R） genes. Pia, c...Blast, caused by Magnaporthe oryzae, is one of the most widespread and destructive diseases of rice. Breeding durable resistant cultivars （cvs） can be achieved by pyramiding of various resistance （R） genes. Pia, carded by cv. Aichi Asahi, was evaluated against 612 isolates of M. oryzae collected from 10 Chinese provinces. The Pia gene expresses weak resistance in all the provinces except for Jiangsu. Genomic position-ready marker-based linkage analysis was carded out in a mapping population consisting of 800 F2 plants derived from a cross of Aichi Asahi×Kasalath. The locus was defined in an interval of approximately 90 kb, flanked by markers A16 and A21. Four candidate genes （Pia-1, Pia-2, Pia-3, and Pia-4）, all having the R gene conserved structure, were predicted in the interval using the cv. Nipponbare genomic sequence. Four candidate resistance gene （CRG） markers （A17, A25, A26, and A27）, derived from the four candidates, were subjected to genotyping with the recombinants detected at the flanking markers. The first three markers completely co-segregated with the Pia locus, and the fourth was absent in the Aichi Asahi genome and disordered with the Pia locus and its flanking markers, indicating that the fourth candidate gene, Pia-4, could be excluded. Co-segregation marker-based genotyping of the three sets of differentials with known R gene genotypes revealed that the genotype of A26 （Pia-3） perfectly matched the R gene genotype of Pia, indicating that Pia-3 is the strongest candidate gene for Pia.展开更多
The Daqingshan thrust system, to the south of the Shiguai Mesozoic basin, is a complex system of top-to- the-north thrusting tectonic sheets. The thrust system has a complicated evolution due to multi-stage thrusting....The Daqingshan thrust system, to the south of the Shiguai Mesozoic basin, is a complex system of top-to- the-north thrusting tectonic sheets. The thrust system has a complicated evolution due to multi-stage thrusting. In order to date the thrusting events, syntectonic muscovite and bio-tite grains are respectively analyzed with normal 40Ar/39Ar dating and laser 40Ar/39Ar dating, which yield 2 isochron ages, i.e. 193.74 3.88 Ma and 121.6 1.6 Ma. These ages suggest that faults within the Daqingshan thrust system formed dur-ing 2 stages of thrusting, one the early Indosinian and the other the late Yanshanian. The isotopic dating is consistent with field geological relations. Indosinan deformation is evi-denced by top-to-the-north thrusting, with the occurrence of a series of large-scale east-west trending thrust faults and folds, while the Yanshanian thrusting is characterized by top-to-the-NNW thrusting. It is superposed on and modifies early Indosinian thrust faults.展开更多
The NNE-trending ductile shear zones in Yiwulü Mountain area were formed in relation to two successive extensional events.The Yiwulü High Temperature Extensional Ductile Shear Zone and the Waziyu Low Tempera...The NNE-trending ductile shear zones in Yiwulü Mountain area were formed in relation to two successive extensional events.The Yiwulü High Temperature Extensional Ductile Shear Zone and the Waziyu Low Temperature Extensional Ductile Shear Zone were related to ductile deformation at higher temperatures and brittle-ductile deformation at lower temperatures,respectively.Both deformations were accompanied by large scale volcanic eruptions and magmatic intrusions.Based on structural analysis of macroscopic and microscopic deformations,and quartz lattice preferred orientations,we show that the early Yiwulü High Temperature Extensional Ductile Shear Zone was resulted from a NE-SW extension at amphibolite facies in the middle crust,whereas the Waziyu Low Temperature Extensional Ductile Shear Zone was due mainly to a NWW-SEE extension at greenschist facies in the upper crust.The SHRIMP zircon age of a syn-tectonic granitic dike emplaced at the late stage of high temperature extension is 155±2 Ma,indicating that the early extensional event took place in the Middle-Late Jurassic.40Ar-39Ar age of muscovite from tectonic schists in the low temperature extensional ductile shear zone is 131.6±1.0 Ma,suggesting that the late extension occurred in the Early Cretaceous.Subsequent overall uplifting succeeded the late extension.The new discovery of the Middle-Late Jurassic NNE-trending extensional ductile shear zone provides evidence constraining the switch of tectonic regimes and Middle Jurassic thinning of lithosphere in the eastern North China Craton.展开更多
Cu-Fe composite oxides were prepared by co-precipitation method and tested for higher alcohol synthesis from syngas. The selectivity to C2＋OH and C6＋OH in alcohol distribution was very high while the methane product...Cu-Fe composite oxides were prepared by co-precipitation method and tested for higher alcohol synthesis from syngas. The selectivity to C2＋OH and C6＋OH in alcohol distribution was very high while the methane product fraction in hydrocarbon distribution was rather low, demonstrating a promising potential in higher alcohols synthesis from syngas. The distribution of alcohols and hydrocarbons approximately obeyed Anderson-Schulz-Flory distribution with similar chain growth probability, indicating alcohols and hydrocarbons derived from the same intermediates. The effects of Cu/Fe molar ratio, reaction temperature and gas hourly space velocity （GHSV） on catalytic performance were studied in detail. The sample with a Cu/Fe molar ratio of 10/1 exhibited the best catalytic performance. Higher reaction temperature accelerated water-gas-shift reaction and led to lower total alcohols selectivity. GHSV showed great effect on catalytic performance and higher GHSV increased the total alcohol selectivity, indicating there existed visible dehydration reaction of alcohol into hydrocarbon.展开更多
The arbitrary space-shape free form deformation （FFD） method developed in this paper is based on non-uniform rational B-splines （NURBS） basis function and used for the integral parameterization of nacelle-pylon ge...The arbitrary space-shape free form deformation （FFD） method developed in this paper is based on non-uniform rational B-splines （NURBS） basis function and used for the integral parameterization of nacelle-pylon geometry. The multi-block structured grid deformation technique is established by Delaunay graph mapping method. The optimization objects of aerodynamic characteristics are evaluated by solving NavierStokes equations on the basis of multi-block structured grid. The advanced particle swarm optimization （PSO） is utilized as search algorithm, which com-bines the Kriging model as surrogate model during optimization. The optimization system is used for optimizing the nacelle location of DLR-F6 wing-body-pylon-nacelle. The results indicate that the aerodynamic interference between the parts is significantly reduced. The optimization design system established in this paper has extensive applications and engineering value.展开更多
Based on improved multi-objective particle swarm optimization（MOPSO） algorithm with principal component analysis（PCA） methodology, an efficient high-dimension multiobjective optimization method is proposed, which,...Based on improved multi-objective particle swarm optimization（MOPSO） algorithm with principal component analysis（PCA） methodology, an efficient high-dimension multiobjective optimization method is proposed, which, as the purpose of this paper, aims to improve the convergence of Pareto front in multi-objective optimization design. The mathematical efficiency,the physical reasonableness and the reliability in dealing with redundant objectives of PCA are verified by typical DTLZ5 test function and multi-objective correlation analysis of supercritical airfoil,and the proposed method is integrated into aircraft multi-disciplinary design（AMDEsign） platform, which contains aerodynamics, stealth and structure weight analysis and optimization module.Then the proposed method is used for the multi-point integrated aerodynamic optimization of a wide-body passenger aircraft, in which the redundant objectives identified by PCA are transformed to optimization constraints, and several design methods are compared. The design results illustrate that the strategy used in this paper is sufficient and multi-point design requirements of the passenger aircraft are reached. The visualization level of non-dominant Pareto set is improved by effectively reducing the dimension without losing the primary feature of the problem.展开更多
Constructing high approximation accuracy surrogate model with lower computational cost has great engineering significance. In this paper, using co-Kriging method, an efficient multi-fidelity surrogate model is constru...Constructing high approximation accuracy surrogate model with lower computational cost has great engineering significance. In this paper, using co-Kriging method, an efficient multi-fidelity surrogate model is constructed based on two independent high and low fidelity samples. Co-Kriging method can use a greater quantity of low-fidelity information to enhance the accuracy of a surrogate of the high-fidelity model by modeling the correlation between high and low fidelity model, thus computational cost of building surrogate model can be greatly reduced. A wing-body problem is taken as an example to compare characteristics of co-Kriging multi-fidelity （CKMF） model with traditional Kriging based multi-fidelity （KMF） model. A sampling convergence of the CKMF model and the KMF model is conducted, and an appropriate sampling design is selected through the sampling convergence analysis. The results indicate that CKMF model has higher approximation accuracy with the same high-fidelity samples, and converges at less high-fidelity samples. A wing-body drag reduction optimization design using genetic algorithm is implemented. Satisfying design results are obtained, which validate the feasibility of CKMF model in engineering design.展开更多
Mitigation of sonic boom to an acceptable stage is a key point for the next generation of supersonic transports. Meanwhile, designing a supersonic aircraft with an ideal ground signature is always the focus of researc...Mitigation of sonic boom to an acceptable stage is a key point for the next generation of supersonic transports. Meanwhile, designing a supersonic aircraft with an ideal ground signature is always the focus of research on sonic boom reduction. This paper presents an inverse design approach to optimize the near-field signature of an aircraft, making it close to the shaped ideal ground signature after the propagation in the atmosphere. Using the Proper Orthogonal Decomposition(POD) method, a guessed input of augmented Burgers equation is inversely achieved. By multiple POD iterations, the guessed ground signatures successively approach the target ground signature until the convergence criteria is reached. Finally, the corresponding equivalent area distribution is calculated from the optimal near-field signature through the classical Whitham F-function theory. To validate this method, an optimization example of Lockheed Martin 1021 is demonstrated. The modified configuration has a fully shaped ground signature and achieves a drop of perceived loudness by 7.94 PLdB. This improvement is achieved via shaping the original near-field signature into wiggles and damping it by atmospheric attenuation. At last, a nonphysical ground signature is set as the target to test the robustness of this inverse design method and shows that this method is robust enough for various inputs.展开更多
This study was designed to investigate the in vivo effects of benzo[a]pyrene （BaP） on hepatic ethoxyresorufin-O-deethylase （EROD） activity and its correlation with cytochrome P4501A （CYP1A） protein levels in Seb...This study was designed to investigate the in vivo effects of benzo[a]pyrene （BaP） on hepatic ethoxyresorufin-O-deethylase （EROD） activity and its correlation with cytochrome P4501A （CYP1A） protein levels in Sebastiscus marmoratus, which were exposed through a water column to BaP （10, 100, 1000 ng/L, respectively） or were treated with intraperitoneal injections of BaP （0.5, 1, 5, 10 mg/kg, respectively） every 7 d. The results showed that after 25 d of waterborne exposure to 1000 ng/L BaP, fish hepatic CYP1A levels and EROD activity were significantly induced. In contrast, EROD activity was not altered 7 d after second intraperitoneal injections, whereas, CYP1A protein levels were increased. Dose-dependent increase of biliary BaP metabolites demonstrated that the catalytic activity of CYP1A was induced by treatment with BaP. The lowest observable effect concentration with regard to biliary BaP metabolites （100 ng/L） was much lower than that with reference to EROD activity （1000 ng/L）. The results suggest that biliary polycyclic aromatic hydrocarbon （PAH） metabolites were shown to better reflect the contamination gradients of PAHs than EROD activity. It appeared to be necessary to measure CYP1A protein levels to complement the EROD activity in relevant toxicological assessments.展开更多
A robust optimization design approach of natural laminar airfoils is developed in this paper. First, the non-uniform rational B-splines （NURBS） free form deformation method based on NURBS basis function is introduce...A robust optimization design approach of natural laminar airfoils is developed in this paper. First, the non-uniform rational B-splines （NURBS） free form deformation method based on NURBS basis function is introduced to the airfoil parameterization. Second, aerodynamic characteristics are evaluated by solving Navier-Stokes equations, and theγ-Reθt transition model coupling with shear-stress transport （SST） turbulent model is introduced to simulate boundary layer transition. A numerical simulation of transition flow around NLF0416 airfoil is conducted to test the code. The comparison between numerical simulation results and wind tunnel test data approves the validity and applicability of the present transition model. Third, the optimization system is set up, which uses the separated particle swarm optimization （SPSO） as search algorithm and combines the Kriging models as surrogate model during optimization. The system is applied to carry out robust design about the uncertainty of lift coefficient and Mach number for NASA NLF-0115 airfoil. The data of optimized airfoil aerodynamic characteristics indicates that the optimized airfoil can maintain laminar flow stably in an uncertain range and has a wider range of low drag.展开更多
Low efficiency is the main obstacle to using prime editing in maize(Zea mays).Recently,prime-editing efficiency was greatly improved in mammalian cells and rice(Oryza sativa)plants by engineering primeediting guide RN...Low efficiency is the main obstacle to using prime editing in maize(Zea mays).Recently,prime-editing efficiency was greatly improved in mammalian cells and rice(Oryza sativa)plants by engineering primeediting guide RNAs(pegRNAs),optimizing the prime editor(PE)protein,and manipulating cellular determinants of prime editing.In this study,we tested PEs optimized via these three strategies in maize.We demonstrated that the ePE5max system,composed of PEmax,epegRNAs(pegRNA-evopreQ.1),nicking single guide RNAs(sgRNAs),and MLH1dn,efficiently generated heritable mutations that conferred resistance to herbicides that inhibit 5-enolpyruvylshikimate-3-phosphate synthase(EPSPS),acetolactate synthase(ALS),or acetyl CoA carboxylase(ACCase)activity.Collectively,we demonstrate that the ePE5max system has sufficient efficiency to generate heritable(homozygous or heterozygous)mutations in maize target genes and that the main obstacle to using PEs in maize has thus been removed.展开更多
Constructing metamodel with global high-fidelity in design space is significant in engineering design. In this paper, a double-stage metamodel （DSM） which integrates advantages of both interpolation metamodel and re...Constructing metamodel with global high-fidelity in design space is significant in engineering design. In this paper, a double-stage metamodel （DSM） which integrates advantages of both interpolation metamodel and regression metamodel is constructed. It takes regression model as the first stage to fit overall distribution of the original model, and then interpolation model of regression model approximation error is used as the second stage to improve accuracy. Under the same conditions and with the same samples, DSM expresses higher fidelity and represents physical characteristics of original model better. Besides, in order to validate DSM characteristics, three examples including Ackley function, airfoil aerodynamic analysis and wing aerodynamic analysis are investigated, In the end, airfoil and wing aerodynamic design optimizations using genetic algorithm are presented to verify the engineering applicability of DSM.展开更多
Carbon dioxide and methane are two main greenhouse gases which are contributed to serious global warming.Fortunately,dry reforming of methane(DRM),a very important reaction developed decades ago,can convert these two ...Carbon dioxide and methane are two main greenhouse gases which are contributed to serious global warming.Fortunately,dry reforming of methane(DRM),a very important reaction developed decades ago,can convert these two major greenhouse gases into value-added syngas or hydrogen.The main problem retarding its industrialization is the seriously coking formation upon the nickel-based catalysts.Herein,a series of confined indium-nickel(In-Ni)intermetallic alloy nanocatalysts(In_(x)Ni@SiO_(2))have been prepared and displayed superior coking resistance for DRM reaction.The sample containing 0.5 wt.%of In loading(In_(0.5)Ni@SiO_(2))shows the best balance of carbon deposition resistance and DRM reactivity even after 430 h long term stability test.The boosted carbon resistance can be ascribed to the confinement of core–shell structure and to the transfer of electrons from Indium to Nickel in In-Ni intermetallic alloys due to the smaller electronegativity of In.Both the silica shell and the increase of electron cloud density on metallic Ni can weaken the ability of Ni to activate C–H bond and decrease the deep cracking process of methane.The reaction over the confined InNi intermetallic alloy nanocatalyst was conformed to the Langmuir-Hinshelwood(L-H)mechanism revealed by in situ diffuse reflectance infrared Fourier transform spectroscopy(in-situ DRIFTS).This work provides a guidance to design high performance coking resistance catalysts for methane dry reforming to efficiently utilize these two main greenhouse gases.展开更多
Eight sets of coastal acoustic tomography (CAT) systems were deployed during November 29 to December 10, 2002 at the coasts on both sides of Tokyo Bay to measure tidal current structures at 15-min interval.Sound trans...Eight sets of coastal acoustic tomography (CAT) systems were deployed during November 29 to December 10, 2002 at the coasts on both sides of Tokyo Bay to measure tidal current structures at 15-min interval.Sound transmission across the Tokyo Bay (between Yokohama and Chiba)was successfully traced,even under severe interference from ship generated wakes and bubbles.Tidal current fields changing from northward to southward flow are well reconstructed by the inverse analysis of travel-time difference data for a period with the best sound transmission condition. It is suggested that the CAT is the most powerful tool to continuously map tidal current fields in the coastal seas with heavy shipping traffic and fisheries activity.展开更多
Hepatitis B virus(HBV) infection is still a worldwide health problem;however,the current antiviral therapies for chronic hepatitis B are limited in efficacy.The outcome of HBV infection is thought to be the result of ...Hepatitis B virus(HBV) infection is still a worldwide health problem;however,the current antiviral therapies for chronic hepatitis B are limited in efficacy.The outcome of HBV infection is thought to be the result of complex interactions between the HBV and the host immune system.While the role of the adaptive immune responses in the resolution of HBV infection has been well characterized,the contribution of innate immune mechanisms remains elusive until recent evidence implicates that HBV appears to activate the innate immune response and this response is important for controlling HBV infection.Here,we review our current understanding of innate immune responses to HBV infection and the multifaceted evasion by the virus and discuss the potential strategies to combat chronic HBV infection via induction and restoration of host innate antiviral responses.展开更多
文摘Introduction There is a downward trend of stroke-related mortality in the USA.By reviewing all published articles on stroke mortality in China,we analysed its trend and possible factors that have influenced the trend.Methods Both English and Chinese literatures were searched on the mortality of stroke or cerebrovascular diseases in China.Potential papers related to this topic were identified from PubMed,Medline,Embase,Cochrane Library,Wanfang Database,SINOMED and China National Knowledge Infrastructure databases.Results Comparing the results from the most recent population-based epidemiological survey and databank from the national Center for Disease Control and Prevention,the age-adjusted stroke mortality rate has shown a downward trend among both urban and rural population in the past 30 years in China.Comparing with 30 years ago,the rate of stroke mortality has decreased by more than 31%in urban/suburban population and 11%in rural population.In men,the age-adjusted stroke mortality rate decreased by 18.9%and in women by 24.9%between 1994 and 2013.Factors that may have contributed to the trend of decreased stroke mortality rate include(1)improved healthcare coverage and healthcare environment;(2)improved treatment options and medical technology;(3)support by government to educate the public on stroke and stroke prevention;and(4)improved public knowledge on stroke.Conclusions The age-adjusted stroke mortality rate in China has shown a downward trend among both urban and rural population in the past 30 years.The major influencing factors that helped in reducing stroke mortality in China included improved healthcare coverage,healthcare environment,the updated treatment options and modern medical technology.
基金supported by grants from National Natural Science Foundation of China(82041025 to S.J.,81822045 to L.L.)Development Programs for COVID-19 of Shanghai Science and Technology Commission(Grant No.20431900401).
文摘The pandemic of coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has posed serious threats to global health and economy,thus calling for the development of safe and effective vaccines.The receptorbinding domain(RBD)in the spike protein of SARS-CoV-2 is responsible for its binding to angiotensin-converting enzyme 2(ACE2)receptor.It contains multiple dominant neutralizing epitopes and serves as an important antigen for the development of COVID-19 vaccines.Here,we showed that immunization of mice with a candidate subunit vaccine consisting of SARS-CoV-2 RBD and Fc fragment of human IgG,as an immunopotentiator,elicited high titer of RBD-specific antibodies with robust neutralizing activity against both pseudotyped and live SARS-CoV-2 infections.The mouse antisera could also effectively neutralize infection by pseudotyped SARS-CoV-2 with several natural mutations in RBD and the IgG extracted from the mouse antisera could also show neutralization against pseudotyped SARS-CoV and SARS-related coronavirus(SARSr-CoV).Vaccination of human ACE2 transgenic mice with RBD-Fc could effectively protect mice from the SARS-CoV-2 challenge.These results suggest that SARS-CoV-2 RBD-Fc has good potential to be further developed as an effective and broad-spectrum vaccine to prevent infection of the current SARS-CoV-2 and its mutants,as well as future emerging SARSr-CoVs and re-emerging SARS-CoV.
基金The authors gratefully acknowledge Professor Matthews Koffas(Rensselaer Polytechnic Institute)and Professor Gregory Stephanopoulos(Massachusetts Institute of Technology)for the generous gift of plasmids pOM-PhCHS-MsCHI,pCDF-trc-RgTAL^syn-Pc4CL^syn and pCA1 in support of this study.This work is supported by startup research funds from Rutgers,The State University of New Jersey.Zhenghong Li is grateful for the Ph.D.fellowship from the China Scholarship Council.
文摘Co-culture engineering is an emerging approach for microbial biosynthesis of a variety of biochemicals.In this study,E.coli-E.coli co-cultures were developed for heterologous biosynthesis of the natural product naringenin.The co-cultures were composed of two independent E.coli strains dedicated to functional expression of different portions of the biosynthetic pathway,respectively.The co-culture biosynthesis was optimized by investigating the effect of carbon source,E.coli strain selection,timing of IPTG induction and the inoculation ratio between the co-culture strains.Compared with the monoculture strategy,the utilization of the designed co-cultures significantly improved the naringenin production,largely due to the reduction of metabolic stress,employment of proper hosts for improving pathway enzyme activities,and flexible adjustment of the relative biosynthetic strength between the coculture strains.The findings of this study extend the applicability of co-culture engineering in complex natural product biosynthesis.
基金supported by National Natural Science Foundation of China (Grant Nos. 90814006, 40972135)Geological Survey of China (Grant No. 1212010510515)
文摘The early Precambrian high-grade metamorphosed basement in the Xi Ulanbulang area, central Inner Mongolia of China, is composed mainly of intermediate granulites and charnockitic gneisses. Both types of the rocks are closely associated spatially and temporally, with a gradual variation between them. In order to understand timing of the high-grade metamorphism, we carried out SHRIMP U-Pb dating of zircons of the rocks. Zircons from the granulites and charnockitic gneisses are similar in structure and age. Zircon cores show magmatic zoning and have ages of 2507-2545 Ma. The ages are interpreted as the forming time of protolith of the granulites and charnockitic gneisses, indicating that a strong magmatism existed at that time in the Yinshan Block. The zircon mantles and rims show homogeneous structures and record a strong granulite facies metamorphism event around 2500 Ma, with a time interval between the metamorphism and magamatism being less than 50 Ma. These suggest that the Western Block was similar to the Eastern Block in tectono-thermal timing at the end of the Neoarchean.
文摘A new compressibility correlation is introduced in the Langtry＇s local variable-based transition model to investigate the phe- nomenon on double wedge shock/boundary layer interactions. The cmnputational analysis compared with experimental data has been made to assess the influence of the wall temperature and the leading edge nose radius on a hypersonic double wedge boundary layer. It has been found that the laminar boundary layer separation occurs on the first ramp. Furthermore, the wall temperature and the leading edge nose radius have remarkable influence on the separation characteristics in the kink. Comparison of the calculated pressure coefficient distribution and the boundary layer profile with the experimental data shows that better results can be achieved when using the modified transition model.
基金supported by the National Transgenic Research Projects (Grant No.2009ZX08009-023B)the National Basic Research Program of China(Grant No.2011CB1007)the National Commonweal Specialized Research Project(Grant No.200803008)
文摘Blast, caused by Magnaporthe oryzae, is one of the most widespread and destructive diseases of rice. Breeding durable resistant cultivars （cvs） can be achieved by pyramiding of various resistance （R） genes. Pia, carded by cv. Aichi Asahi, was evaluated against 612 isolates of M. oryzae collected from 10 Chinese provinces. The Pia gene expresses weak resistance in all the provinces except for Jiangsu. Genomic position-ready marker-based linkage analysis was carded out in a mapping population consisting of 800 F2 plants derived from a cross of Aichi Asahi×Kasalath. The locus was defined in an interval of approximately 90 kb, flanked by markers A16 and A21. Four candidate genes （Pia-1, Pia-2, Pia-3, and Pia-4）, all having the R gene conserved structure, were predicted in the interval using the cv. Nipponbare genomic sequence. Four candidate resistance gene （CRG） markers （A17, A25, A26, and A27）, derived from the four candidates, were subjected to genotyping with the recombinants detected at the flanking markers. The first three markers completely co-segregated with the Pia locus, and the fourth was absent in the Aichi Asahi genome and disordered with the Pia locus and its flanking markers, indicating that the fourth candidate gene, Pia-4, could be excluded. Co-segregation marker-based genotyping of the three sets of differentials with known R gene genotypes revealed that the genotype of A26 （Pia-3） perfectly matched the R gene genotype of Pia, indicating that Pia-3 is the strongest candidate gene for Pia.
基金supported by the China Geological Survey Project(Grant No.2000113000021) the Innovation Fund of Jilin University(Grant No.450011022009).
文摘The Daqingshan thrust system, to the south of the Shiguai Mesozoic basin, is a complex system of top-to- the-north thrusting tectonic sheets. The thrust system has a complicated evolution due to multi-stage thrusting. In order to date the thrusting events, syntectonic muscovite and bio-tite grains are respectively analyzed with normal 40Ar/39Ar dating and laser 40Ar/39Ar dating, which yield 2 isochron ages, i.e. 193.74 3.88 Ma and 121.6 1.6 Ma. These ages suggest that faults within the Daqingshan thrust system formed dur-ing 2 stages of thrusting, one the early Indosinian and the other the late Yanshanian. The isotopic dating is consistent with field geological relations. Indosinan deformation is evi-denced by top-to-the-north thrusting, with the occurrence of a series of large-scale east-west trending thrust faults and folds, while the Yanshanian thrusting is characterized by top-to-the-NNW thrusting. It is superposed on and modifies early Indosinian thrust faults.
基金supported by National Natural Science Foundation of China (Grant Nos. 90814006 and 40972135)China Geological Survey Project (Grant No. 1212011085473)
文摘The NNE-trending ductile shear zones in Yiwulü Mountain area were formed in relation to two successive extensional events.The Yiwulü High Temperature Extensional Ductile Shear Zone and the Waziyu Low Temperature Extensional Ductile Shear Zone were related to ductile deformation at higher temperatures and brittle-ductile deformation at lower temperatures,respectively.Both deformations were accompanied by large scale volcanic eruptions and magmatic intrusions.Based on structural analysis of macroscopic and microscopic deformations,and quartz lattice preferred orientations,we show that the early Yiwulü High Temperature Extensional Ductile Shear Zone was resulted from a NE-SW extension at amphibolite facies in the middle crust,whereas the Waziyu Low Temperature Extensional Ductile Shear Zone was due mainly to a NWW-SEE extension at greenschist facies in the upper crust.The SHRIMP zircon age of a syn-tectonic granitic dike emplaced at the late stage of high temperature extension is 155±2 Ma,indicating that the early extensional event took place in the Middle-Late Jurassic.40Ar-39Ar age of muscovite from tectonic schists in the low temperature extensional ductile shear zone is 131.6±1.0 Ma,suggesting that the late extension occurred in the Early Cretaceous.Subsequent overall uplifting succeeded the late extension.The new discovery of the Middle-Late Jurassic NNE-trending extensional ductile shear zone provides evidence constraining the switch of tectonic regimes and Middle Jurassic thinning of lithosphere in the eastern North China Craton.
基金the State Key Fundamental Research Program(Ministry of Science and Technology of China,No.2011CBA00501)Shanghai Municipal Science and Technology Commission,China(Grant No:11DZ1200300)the Foundation of State Key Laboratory of Coal Conversion(Grant No:1112610)
文摘Cu-Fe composite oxides were prepared by co-precipitation method and tested for higher alcohol synthesis from syngas. The selectivity to C2＋OH and C6＋OH in alcohol distribution was very high while the methane product fraction in hydrocarbon distribution was rather low, demonstrating a promising potential in higher alcohols synthesis from syngas. The distribution of alcohols and hydrocarbons approximately obeyed Anderson-Schulz-Flory distribution with similar chain growth probability, indicating alcohols and hydrocarbons derived from the same intermediates. The effects of Cu/Fe molar ratio, reaction temperature and gas hourly space velocity （GHSV） on catalytic performance were studied in detail. The sample with a Cu/Fe molar ratio of 10/1 exhibited the best catalytic performance. Higher reaction temperature accelerated water-gas-shift reaction and led to lower total alcohols selectivity. GHSV showed great effect on catalytic performance and higher GHSV increased the total alcohol selectivity, indicating there existed visible dehydration reaction of alcohol into hydrocarbon.
文摘The arbitrary space-shape free form deformation （FFD） method developed in this paper is based on non-uniform rational B-splines （NURBS） basis function and used for the integral parameterization of nacelle-pylon geometry. The multi-block structured grid deformation technique is established by Delaunay graph mapping method. The optimization objects of aerodynamic characteristics are evaluated by solving NavierStokes equations on the basis of multi-block structured grid. The advanced particle swarm optimization （PSO） is utilized as search algorithm, which com-bines the Kriging model as surrogate model during optimization. The optimization system is used for optimizing the nacelle location of DLR-F6 wing-body-pylon-nacelle. The results indicate that the aerodynamic interference between the parts is significantly reduced. The optimization design system established in this paper has extensive applications and engineering value.
基金supported by the National Natural Science Foundation of China (No.11402288)
文摘Based on improved multi-objective particle swarm optimization（MOPSO） algorithm with principal component analysis（PCA） methodology, an efficient high-dimension multiobjective optimization method is proposed, which, as the purpose of this paper, aims to improve the convergence of Pareto front in multi-objective optimization design. The mathematical efficiency,the physical reasonableness and the reliability in dealing with redundant objectives of PCA are verified by typical DTLZ5 test function and multi-objective correlation analysis of supercritical airfoil,and the proposed method is integrated into aircraft multi-disciplinary design（AMDEsign） platform, which contains aerodynamics, stealth and structure weight analysis and optimization module.Then the proposed method is used for the multi-point integrated aerodynamic optimization of a wide-body passenger aircraft, in which the redundant objectives identified by PCA are transformed to optimization constraints, and several design methods are compared. The design results illustrate that the strategy used in this paper is sufficient and multi-point design requirements of the passenger aircraft are reached. The visualization level of non-dominant Pareto set is improved by effectively reducing the dimension without losing the primary feature of the problem.
基金supported by the Seventh Framework Programme of China-EU Collaborative Projects
文摘Constructing high approximation accuracy surrogate model with lower computational cost has great engineering significance. In this paper, using co-Kriging method, an efficient multi-fidelity surrogate model is constructed based on two independent high and low fidelity samples. Co-Kriging method can use a greater quantity of low-fidelity information to enhance the accuracy of a surrogate of the high-fidelity model by modeling the correlation between high and low fidelity model, thus computational cost of building surrogate model can be greatly reduced. A wing-body problem is taken as an example to compare characteristics of co-Kriging multi-fidelity （CKMF） model with traditional Kriging based multi-fidelity （KMF） model. A sampling convergence of the CKMF model and the KMF model is conducted, and an appropriate sampling design is selected through the sampling convergence analysis. The results indicate that CKMF model has higher approximation accuracy with the same high-fidelity samples, and converges at less high-fidelity samples. A wing-body drag reduction optimization design using genetic algorithm is implemented. Satisfying design results are obtained, which validate the feasibility of CKMF model in engineering design.
文摘Mitigation of sonic boom to an acceptable stage is a key point for the next generation of supersonic transports. Meanwhile, designing a supersonic aircraft with an ideal ground signature is always the focus of research on sonic boom reduction. This paper presents an inverse design approach to optimize the near-field signature of an aircraft, making it close to the shaped ideal ground signature after the propagation in the atmosphere. Using the Proper Orthogonal Decomposition(POD) method, a guessed input of augmented Burgers equation is inversely achieved. By multiple POD iterations, the guessed ground signatures successively approach the target ground signature until the convergence criteria is reached. Finally, the corresponding equivalent area distribution is calculated from the optimal near-field signature through the classical Whitham F-function theory. To validate this method, an optimization example of Lockheed Martin 1021 is demonstrated. The modified configuration has a fully shaped ground signature and achieves a drop of perceived loudness by 7.94 PLdB. This improvement is achieved via shaping the original near-field signature into wiggles and damping it by atmospheric attenuation. At last, a nonphysical ground signature is set as the target to test the robustness of this inverse design method and shows that this method is robust enough for various inputs.
基金This work was supported by the National Natural Science Foundation of China(No.40476049).
文摘This study was designed to investigate the in vivo effects of benzo[a]pyrene （BaP） on hepatic ethoxyresorufin-O-deethylase （EROD） activity and its correlation with cytochrome P4501A （CYP1A） protein levels in Sebastiscus marmoratus, which were exposed through a water column to BaP （10, 100, 1000 ng/L, respectively） or were treated with intraperitoneal injections of BaP （0.5, 1, 5, 10 mg/kg, respectively） every 7 d. The results showed that after 25 d of waterborne exposure to 1000 ng/L BaP, fish hepatic CYP1A levels and EROD activity were significantly induced. In contrast, EROD activity was not altered 7 d after second intraperitoneal injections, whereas, CYP1A protein levels were increased. Dose-dependent increase of biliary BaP metabolites demonstrated that the catalytic activity of CYP1A was induced by treatment with BaP. The lowest observable effect concentration with regard to biliary BaP metabolites （100 ng/L） was much lower than that with reference to EROD activity （1000 ng/L）. The results suggest that biliary polycyclic aromatic hydrocarbon （PAH） metabolites were shown to better reflect the contamination gradients of PAHs than EROD activity. It appeared to be necessary to measure CYP1A protein levels to complement the EROD activity in relevant toxicological assessments.
文摘A robust optimization design approach of natural laminar airfoils is developed in this paper. First, the non-uniform rational B-splines （NURBS） free form deformation method based on NURBS basis function is introduced to the airfoil parameterization. Second, aerodynamic characteristics are evaluated by solving Navier-Stokes equations, and theγ-Reθt transition model coupling with shear-stress transport （SST） turbulent model is introduced to simulate boundary layer transition. A numerical simulation of transition flow around NLF0416 airfoil is conducted to test the code. The comparison between numerical simulation results and wind tunnel test data approves the validity and applicability of the present transition model. Third, the optimization system is set up, which uses the separated particle swarm optimization （SPSO） as search algorithm and combines the Kriging models as surrogate model during optimization. The system is applied to carry out robust design about the uncertainty of lift coefficient and Mach number for NASA NLF-0115 airfoil. The data of optimized airfoil aerodynamic characteristics indicates that the optimized airfoil can maintain laminar flow stably in an uncertain range and has a wider range of low drag.
基金supported by grants from the National Natural Science Foundation of China(grant nos.31872678 and U19A2022)。
文摘Low efficiency is the main obstacle to using prime editing in maize(Zea mays).Recently,prime-editing efficiency was greatly improved in mammalian cells and rice(Oryza sativa)plants by engineering primeediting guide RNAs(pegRNAs),optimizing the prime editor(PE)protein,and manipulating cellular determinants of prime editing.In this study,we tested PEs optimized via these three strategies in maize.We demonstrated that the ePE5max system,composed of PEmax,epegRNAs(pegRNA-evopreQ.1),nicking single guide RNAs(sgRNAs),and MLH1dn,efficiently generated heritable mutations that conferred resistance to herbicides that inhibit 5-enolpyruvylshikimate-3-phosphate synthase(EPSPS),acetolactate synthase(ALS),or acetyl CoA carboxylase(ACCase)activity.Collectively,we demonstrate that the ePE5max system has sufficient efficiency to generate heritable(homozygous or heterozygous)mutations in maize target genes and that the main obstacle to using PEs in maize has thus been removed.
文摘Constructing metamodel with global high-fidelity in design space is significant in engineering design. In this paper, a double-stage metamodel （DSM） which integrates advantages of both interpolation metamodel and regression metamodel is constructed. It takes regression model as the first stage to fit overall distribution of the original model, and then interpolation model of regression model approximation error is used as the second stage to improve accuracy. Under the same conditions and with the same samples, DSM expresses higher fidelity and represents physical characteristics of original model better. Besides, in order to validate DSM characteristics, three examples including Ackley function, airfoil aerodynamic analysis and wing aerodynamic analysis are investigated, In the end, airfoil and wing aerodynamic design optimizations using genetic algorithm are presented to verify the engineering applicability of DSM.
基金supported by the National Natural Science Foundation of China(21976078 and 21773106)the National Key R&D Program of China(2016YFC0205900)+1 种基金the Natural Science Foundation of Jiangxi Province(20202ACB213001)National Engineering Laboratory for Mobile Source Emission Control Technology(NELMS2019A12)。
文摘Carbon dioxide and methane are two main greenhouse gases which are contributed to serious global warming.Fortunately,dry reforming of methane(DRM),a very important reaction developed decades ago,can convert these two major greenhouse gases into value-added syngas or hydrogen.The main problem retarding its industrialization is the seriously coking formation upon the nickel-based catalysts.Herein,a series of confined indium-nickel(In-Ni)intermetallic alloy nanocatalysts(In_(x)Ni@SiO_(2))have been prepared and displayed superior coking resistance for DRM reaction.The sample containing 0.5 wt.%of In loading(In_(0.5)Ni@SiO_(2))shows the best balance of carbon deposition resistance and DRM reactivity even after 430 h long term stability test.The boosted carbon resistance can be ascribed to the confinement of core–shell structure and to the transfer of electrons from Indium to Nickel in In-Ni intermetallic alloys due to the smaller electronegativity of In.Both the silica shell and the increase of electron cloud density on metallic Ni can weaken the ability of Ni to activate C–H bond and decrease the deep cracking process of methane.The reaction over the confined InNi intermetallic alloy nanocatalyst was conformed to the Langmuir-Hinshelwood(L-H)mechanism revealed by in situ diffuse reflectance infrared Fourier transform spectroscopy(in-situ DRIFTS).This work provides a guidance to design high performance coking resistance catalysts for methane dry reforming to efficiently utilize these two main greenhouse gases.
文摘Eight sets of coastal acoustic tomography (CAT) systems were deployed during November 29 to December 10, 2002 at the coasts on both sides of Tokyo Bay to measure tidal current structures at 15-min interval.Sound transmission across the Tokyo Bay (between Yokohama and Chiba)was successfully traced,even under severe interference from ship generated wakes and bubbles.Tidal current fields changing from northward to southward flow are well reconstructed by the inverse analysis of travel-time difference data for a period with the best sound transmission condition. It is suggested that the CAT is the most powerful tool to continuously map tidal current fields in the coastal seas with heavy shipping traffic and fisheries activity.
基金supported by the German ResearchFoundation(SFB/Transregio TRR60)the InternationalScience&Technology Cooperation Program of China(Grant 2011DFA31030)the National Key BasicResearch Program of China(2012CB519005)
文摘Hepatitis B virus(HBV) infection is still a worldwide health problem;however,the current antiviral therapies for chronic hepatitis B are limited in efficacy.The outcome of HBV infection is thought to be the result of complex interactions between the HBV and the host immune system.While the role of the adaptive immune responses in the resolution of HBV infection has been well characterized,the contribution of innate immune mechanisms remains elusive until recent evidence implicates that HBV appears to activate the innate immune response and this response is important for controlling HBV infection.Here,we review our current understanding of innate immune responses to HBV infection and the multifaceted evasion by the virus and discuss the potential strategies to combat chronic HBV infection via induction and restoration of host innate antiviral responses.
