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烈性青枯雷尔氏菌噬菌体的分离与生物学特性分析 被引量:8
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作者 苏靖芳 孙剑萍 +4 位作者 顾刚 刘俊杰 孙宏伟 郭兆奎 王光华 《烟草科技》 EI CAS CSCD 北大核心 2017年第10期16-21,共6页
为采用噬菌体疗法防治烟草青枯病,以烟草青枯菌TB15-15为宿主菌,从土壤中筛选分离得到噬菌体,并采用双层平板法检测了噬菌体的效价、最佳感染复数(MOI)、一步生长曲线以及噬菌体对温度和酸碱度的敏感性。结果表明:从长期植烟田块土壤中... 为采用噬菌体疗法防治烟草青枯病,以烟草青枯菌TB15-15为宿主菌,从土壤中筛选分离得到噬菌体,并采用双层平板法检测了噬菌体的效价、最佳感染复数(MOI)、一步生长曲线以及噬菌体对温度和酸碱度的敏感性。结果表明:从长期植烟田块土壤中分离得到1株青枯雷尔氏菌烈性噬菌体,命名为RS-PⅡ-1。经检测该噬菌体最佳感染复数为1;一步生长曲线显示噬菌体潜伏期为10 min,爆发期持续100 min,裂解量为1 411;该噬菌体40℃活性最高,裂解量也最大,当温度超过40℃时活性逐渐下降,直至在60℃时活性丧失;噬菌体对酸碱均具有一定的耐受能力,pH在4~8范围内噬菌体均有很好裂解活性,当p H小于4或大于8,裂解活性均减小,特别是在p H大于11时,噬菌体裂解活性完全丧失,因此噬菌体RS-PⅡ-1不适合与碱性溶液混合应用。 展开更多
关键词 烟草 青枯病 青枯雷尔氏菌 噬菌体 生物学特性
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Self-organizing strategy design and validation forintegrated air-ground detection swarm
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作者 Meiyan An zhaokui wang Yulin Zhang 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2016年第5期1018-1027,共10页
A self-organized integrated air-ground detection swarmis tentatively applied to achieve reentry vehicle landing detection,such as searching and rescuing a manned spaceship. The detectionswarm consists of multiple unma... A self-organized integrated air-ground detection swarmis tentatively applied to achieve reentry vehicle landing detection,such as searching and rescuing a manned spaceship. The detectionswarm consists of multiple unmanned aerial vehicles (UAVs)and unmanned ground vehicles (UGVs). The UAVs can accessa detected object quickly for high mobility, while the UGVs cancomprehensively investigate the object due to the variety of carriedequipment. In addition, the integrated air-ground detectionswarm is capable of detecting from the ground and the air simultaneously.To accomplish the coordination of the UGVs andUAVs, they are all regarded as individuals of the artificial swarm.Those individuals make control decisions independently of othersbased on the self-organizing strategy. The overall requirements forthe detection swarm are analyzed, and the theoretical model ofthe self-organizing strategy based on a combined individual andenvironmental virtual function is established. The numerical investigationproves that the self-organizing strategy is suitable andscalable to control the detection swarm. To further inspect the engineeringreliability, an experiment set is established in laboratory,and the experimental demonstration shows that the self-organizingstrategy drives the detection swarm forming a close range and multiangularsurveillance configuration of a landing spot. 展开更多
关键词 artificial swarm virtual potential field self-organizing integrated air-ground detection swarm.
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CAE在自动变速器壳体上的应用
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作者 张兆奎 宋文福 +2 位作者 王悦 李晓宇 王君 《汽车实用技术》 2016年第11期82-83,89,共3页
文章自动变速器壳体不但承受来自内部轴承、制动器的作用力,而且受到对接发动机和悬置的螺栓轴力,因此对变速器的承载能力一定的要求。本文采用有限元法建立分析模型,对自动变速器各个档位工况进行负荷加载,考察其强度和变形情况,为壳... 文章自动变速器壳体不但承受来自内部轴承、制动器的作用力,而且受到对接发动机和悬置的螺栓轴力,因此对变速器的承载能力一定的要求。本文采用有限元法建立分析模型,对自动变速器各个档位工况进行负荷加载,考察其强度和变形情况,为壳体初始设计提供一定的依据。 展开更多
关键词 自动变速器 有限元分析 ABAQUS
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