The LIGO detection of gravitational waves(GW) from merging black holes in 2015 marked the beginning of a new era in observational astronomy. The detection of an electromagnetic signal from a GW source is the critical ...The LIGO detection of gravitational waves(GW) from merging black holes in 2015 marked the beginning of a new era in observational astronomy. The detection of an electromagnetic signal from a GW source is the critical next step to explore in detail the physics involved. The Antarctic Survey Telescopes(AST3),located at Dome A, Antarctica, is uniquely situated for rapid response time-domain astronomy with its continuous night-time coverage during the austral winter. We report optical observations of the GW source(GW 170817) in the nearby galaxy NGC 4993 using AST3. The data show a rapidly fading transient at around 1 day after the GW trigger, with the i-band magnitude declining from 17:23 ± 0:13 magnitude to 17:72 ± 0:09 magnitude in ~1:8 h. The brightness and time evolution of the optical transient associated with GW 170817 are broadly consistent with the predictions of models involving merging binary neutron stars. We infer from our data that the merging process ejected about ~10^(-2) solar mass of radioactive material at a speed of up to 30% the speed of light.展开更多
目的观察理筋正骨手法结合肌内效贴治疗急性期肩关节周围炎的临床疗效。方法选择60例急性期肩关节周围炎患者,随机分为手法组和对照组,每组30例。手法组给予理筋正骨手法结合肌内效贴治疗,对照组给予超短波疗法结合肌内效贴治疗。比较...目的观察理筋正骨手法结合肌内效贴治疗急性期肩关节周围炎的临床疗效。方法选择60例急性期肩关节周围炎患者,随机分为手法组和对照组,每组30例。手法组给予理筋正骨手法结合肌内效贴治疗,对照组给予超短波疗法结合肌内效贴治疗。比较两组患者临床疗效及治疗前后视觉模拟量表(visual analog scale,VAS)评分、压痛阈值、肩关节活动度(range of motion,ROM)、肩关节功能Constant-Murley评分(Constant-Murley score,CMS)、表面肌电积分肌电图(integral electromyogram,iEMG)值。结果手法组疗效优于对照组(P<0.05);两组治疗后VAS评分均显著降低,且手法组降低程度大于对照组(P<0.05);两组治疗后压痛阈值、CMS、肩关节ROM、iEMG值均显著升高(P<0.05),且手法组升高程度大于对照组(P<0.05)。结论理筋正骨手法结合肌内效贴治疗可缓解急性期肩关节周围炎患者的疼痛,改善肩关节功能。展开更多
Ketogulonigenium sp. may convert L-sorbose into 2-keto-L-gulonic acid, the vitamin C precursor. The genome of Ketogulonigenium sp. WB0104 consists of a circular 2765030 bp chromo- some with 61.69% G+C content and two ...Ketogulonigenium sp. may convert L-sorbose into 2-keto-L-gulonic acid, the vitamin C precursor. The genome of Ketogulonigenium sp. WB0104 consists of a circular 2765030 bp chromo- some with 61.69% G+C content and two circular plasmids of 267968 and 242707 bp. The genome contains 2727 open reading frames (ORFs). The systems of replication, transcription, translation, carbohydrate and energy metabolism are intact, but the repair system is incomplete. About 640 predicted ORFs have been found to encode transporter pro- teins, which account for about one fourth of total predicted ORFs, noticeably higher than other docu- mented bacteria. This may be due to the fact that WB0104 adapts to soil circumstance.展开更多
Nasopharyngeal carcinoma(NPC)is a serious and highly invasive epithelial malignancy that is closely associated with Epstein‒Barr virus(EBV).Due to the lack of therapeutic vaccines for NPC,we selected EBV latent membra...Nasopharyngeal carcinoma(NPC)is a serious and highly invasive epithelial malignancy that is closely associated with Epstein‒Barr virus(EBV).Due to the lack of therapeutic vaccines for NPC,we selected EBV latent membrane protein 2(LMP2)as a preferable targeting antigen to develop a lipid-based LMP2-mRNA(mLMP2)vaccine.Full-length mLMP2 expressing LMP2 was first synthesized using an in vitro transcription method and then encapsulated into(2,3-dioleacyl propyl)trimethylammonium chloride(DOTAP)-based cationic liposomes to obtain the mRNA vaccine(LPX-mLMP2).The cell assays showed that the antigenpresenting cells were capable of highly efficient uptake of LPX-mLMP2 and expression of LMP2.LMP2 could subsequently be presented to form the peptide-major histocompatibility complex(pMHC).Furthermore,LPX-mLMP2 could accumulate in the spleen,express antigens,promote the maturation of dendritic cells and stimulate antigen-specific T-cell responses in vivo.It dramatically inhibited the tumor growth of the LMP2-expressing tumor model after three doses of vaccination.Additionally,the proliferation of antigen-specific T cells in the tumor site made a good sign for the promise of mRNA vaccines in virus-induced cancer.Overall,we provided a newly developed antigen-encoding mRNA vaccine with advantages against NPC.We also demonstrated that mRNA vaccines are attractive candidates for cancer immunotherapy.展开更多
目的研究冬连三七组分(Composition of Ophiopogon polysaccharide,Notoginseng total saponins and Rhizoma Coptidis alkaloids CONR)调控AGEs/p38MAPK信号转导通路改善糖尿病动脉粥样硬化(diabetic atherosclerosis,DA)兔氧化应激反...目的研究冬连三七组分(Composition of Ophiopogon polysaccharide,Notoginseng total saponins and Rhizoma Coptidis alkaloids CONR)调控AGEs/p38MAPK信号转导通路改善糖尿病动脉粥样硬化(diabetic atherosclerosis,DA)兔氧化应激反应。方法设立空白组、模型组、辛伐他汀组、CONR高剂量组、CONR低剂量组、SB203580组;脉推注四氧嘧啶并且配合腹主动脉内膜球囊损伤术诱导雄性纯种新西兰大白兔的DA模型,实验组分别每日给予CONR450 mg/kg、CONR 150 mg/kg;辛伐他汀3 mg/kg灌胃10周,12 h重复腹腔注射SB203580 2 mg/kg10周;正常组每日给予生理盐水20mL灌胃作为对照。结果实验各组对AGEs均有降低作用,对p-p38MAPK蛋白高表达均有抑制作用,与模型组比较差异有统计学意义。CONR高、低剂量组、辛伐他汀组对p-p38MAPK蛋白的抑制作用优于SB203580组(P<0.01),CONR高、CONR低剂量组、辛伐他汀组之间差异无统计学意义(P>0.05)。模型组大兔血清MDA高于空白组,有统计学差异(P<0.01)。给药后,CONR高剂量组、SB203580组及辛伐他汀组显著降低MDA(P<0.01)。模型组SOD活性显著降低(P<0.01),实验各组显著增强SOD活性(P<0.01)。结论冬连三七组分可能通过干预AGEs/p38MAPK信号转导通路改善DA兔的氧化应激反应。展开更多
In the comparison with SARS-CoVof 2003,SARS-CoV-2 is extremely well adapted to the human populations and its adaptive shift from the animal host to humans must have been even more extensive.By the blind watchmaker arg...In the comparison with SARS-CoVof 2003,SARS-CoV-2 is extremely well adapted to the human populations and its adaptive shift from the animal host to humans must have been even more extensive.By the blind watchmaker argument,such an adaptive shift can only happen prior to the onset of the current pandemic and with the aid of step-by-step selection.展开更多
基金supported by the National Basic Research Program(973 Program)of China(2013CB834900)the Chinese Polar Environment Comprehensive Investigation&Assessment Program(CHINARE2016-02-03)+21 种基金the National Natural Science Foundation of China(11573014,11673068,11325313,11633002,11433009,11725314)the Key Research Program of Frontier Sciences(QYZDY-SSW-SLH010,QYZDB-SSW-SYS005)the Strategic Priority Research Program"multi-waveband gravitational wave Universe”(XDB23040000)the Youth Innovation Promotion Association(2011231)of Chinese Academy of Sciencesfunds from Tsinghua UniversityNanjing UniversityBeijing Normal UniversityUniversity of New South WalesTexas A&M Universitythe Australian Antarctic Divisionthe National Collaborative Research Infrastructure Strategy(NCRIS)of Australiafunding from the Chinese Academy of Sciences through the Center for Astronomical Mega-Science and National Astronomical Observatory of China(NAOC)made possible through the use of the AAVSO Photometric All-Sky Survey(APASS)funded by the Robert Martin Ayers Sciences Fundfunded by the Australian Research Council(ARC)Centre of Excellence for Gravitational Wave Discovery(OzGrav),CE170100004the ARC Centre of Excellence for All-sky Astrophysics(CAASTRO),CE110001020the Centre of Excellence for All-sky Astrophysics in 3-Dimensions(ASTRO-3D),CE170100013provided by the Australian Astronomical Observatory(AAO)the ARC Future Fellowship grant,FT130101219supported by the National Basic Research Program(Project 973)of China(2014CB845800)the National Natural Science Foundation of China(11633001 and 11373014)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB23000000)
文摘The LIGO detection of gravitational waves(GW) from merging black holes in 2015 marked the beginning of a new era in observational astronomy. The detection of an electromagnetic signal from a GW source is the critical next step to explore in detail the physics involved. The Antarctic Survey Telescopes(AST3),located at Dome A, Antarctica, is uniquely situated for rapid response time-domain astronomy with its continuous night-time coverage during the austral winter. We report optical observations of the GW source(GW 170817) in the nearby galaxy NGC 4993 using AST3. The data show a rapidly fading transient at around 1 day after the GW trigger, with the i-band magnitude declining from 17:23 ± 0:13 magnitude to 17:72 ± 0:09 magnitude in ~1:8 h. The brightness and time evolution of the optical transient associated with GW 170817 are broadly consistent with the predictions of models involving merging binary neutron stars. We infer from our data that the merging process ejected about ~10^(-2) solar mass of radioactive material at a speed of up to 30% the speed of light.
文摘目的观察理筋正骨手法结合肌内效贴治疗急性期肩关节周围炎的临床疗效。方法选择60例急性期肩关节周围炎患者,随机分为手法组和对照组,每组30例。手法组给予理筋正骨手法结合肌内效贴治疗,对照组给予超短波疗法结合肌内效贴治疗。比较两组患者临床疗效及治疗前后视觉模拟量表(visual analog scale,VAS)评分、压痛阈值、肩关节活动度(range of motion,ROM)、肩关节功能Constant-Murley评分(Constant-Murley score,CMS)、表面肌电积分肌电图(integral electromyogram,iEMG)值。结果手法组疗效优于对照组(P<0.05);两组治疗后VAS评分均显著降低,且手法组降低程度大于对照组(P<0.05);两组治疗后压痛阈值、CMS、肩关节ROM、iEMG值均显著升高(P<0.05),且手法组升高程度大于对照组(P<0.05)。结论理筋正骨手法结合肌内效贴治疗可缓解急性期肩关节周围炎患者的疼痛,改善肩关节功能。
文摘Ketogulonigenium sp. may convert L-sorbose into 2-keto-L-gulonic acid, the vitamin C precursor. The genome of Ketogulonigenium sp. WB0104 consists of a circular 2765030 bp chromo- some with 61.69% G+C content and two circular plasmids of 267968 and 242707 bp. The genome contains 2727 open reading frames (ORFs). The systems of replication, transcription, translation, carbohydrate and energy metabolism are intact, but the repair system is incomplete. About 640 predicted ORFs have been found to encode transporter pro- teins, which account for about one fourth of total predicted ORFs, noticeably higher than other docu- mented bacteria. This may be due to the fact that WB0104 adapts to soil circumstance.
基金supported by the National Key Research and Development Program of China(No.2021YFE0206600)Sichuan Province Science and Technology Support Program(Nos.2021YFSY0008 and 2020YFH0065)+1 种基金the Translational medicine fund of West China Hospital(No.CGZH19002)1.3.5 project for disciplines of excellence,West China Hospital,Sichuan University(No.ZYGD18020/ZYJC18006).
文摘Nasopharyngeal carcinoma(NPC)is a serious and highly invasive epithelial malignancy that is closely associated with Epstein‒Barr virus(EBV).Due to the lack of therapeutic vaccines for NPC,we selected EBV latent membrane protein 2(LMP2)as a preferable targeting antigen to develop a lipid-based LMP2-mRNA(mLMP2)vaccine.Full-length mLMP2 expressing LMP2 was first synthesized using an in vitro transcription method and then encapsulated into(2,3-dioleacyl propyl)trimethylammonium chloride(DOTAP)-based cationic liposomes to obtain the mRNA vaccine(LPX-mLMP2).The cell assays showed that the antigenpresenting cells were capable of highly efficient uptake of LPX-mLMP2 and expression of LMP2.LMP2 could subsequently be presented to form the peptide-major histocompatibility complex(pMHC).Furthermore,LPX-mLMP2 could accumulate in the spleen,express antigens,promote the maturation of dendritic cells and stimulate antigen-specific T-cell responses in vivo.It dramatically inhibited the tumor growth of the LMP2-expressing tumor model after three doses of vaccination.Additionally,the proliferation of antigen-specific T cells in the tumor site made a good sign for the promise of mRNA vaccines in virus-induced cancer.Overall,we provided a newly developed antigen-encoding mRNA vaccine with advantages against NPC.We also demonstrated that mRNA vaccines are attractive candidates for cancer immunotherapy.
文摘In the comparison with SARS-CoVof 2003,SARS-CoV-2 is extremely well adapted to the human populations and its adaptive shift from the animal host to humans must have been even more extensive.By the blind watchmaker argument,such an adaptive shift can only happen prior to the onset of the current pandemic and with the aid of step-by-step selection.
