The Agrobacterium-mediated transformation system is the most commonly used method in soybean transformation.Screening of soybean genotypes favorable for Agrobacterium-infection and tissue regeneration is the most impo...The Agrobacterium-mediated transformation system is the most commonly used method in soybean transformation.Screening of soybean genotypes favorable for Agrobacterium-infection and tissue regeneration is the most important step to establish an efficient genetic transformation system.In this study,twenty soybean genotypes that originated from different soybean production regions in China were screened for transient infection,regeneration capacity,and stable transgenic efficiency.Three genotypes,Yuechun 04-5,Yuechun 03-3,and Tianlong 1,showed comparable stable transgenic efficiencies with that of the previously reported American genotypes Williams 82 and Jack in our experimental system.For the Tianlong 1,the average stable transformation efficiency is 4.59%,higher than that of control genotypes(Jack and Williams 82),which is enough for further genomic research and genetic engineering.While polymerase chain reaction(PCR),LibertyLink strips,and β-glucuronidase(GUS) staining assays were used to detect the insertion and expression of the transgene,leaves painted with 135 mg/L Basta could efficiently identify the transformants.展开更多
目的 系统分析纸牌游戏在预立医疗照护计划中的应用研究,旨在为相关研究及其临床推广应用提供参考。方法 按照范围综述的研究方法,计算机检索中国知网、万方数据库、中国生物医学文献数据库、Cochrane Library、Embase、Medline、Web of...目的 系统分析纸牌游戏在预立医疗照护计划中的应用研究,旨在为相关研究及其临床推广应用提供参考。方法 按照范围综述的研究方法,计算机检索中国知网、万方数据库、中国生物医学文献数据库、Cochrane Library、Embase、Medline、Web of Science、PubMed,检索时限为建库至2021年10月2日。收集纸牌游戏应用于预立医疗照护计划中的相关研究。结果 共纳入11篇文献,包括4种纸牌游戏,即My gift of grace纸牌游戏、Heart to Heart纸牌游戏、The Life Unlocking纸牌游戏和Go Wish纸牌游戏。其在促进临终讨论、确定临终偏好、提高预立医疗照护计划相关行为的参与度等方面显示出积极作用。结论 纸牌游戏在预立医疗照护计划中显示出积极的应用效果和可行性,今后还需要多中心、大样本、高质量研究进一步明确其在我国的适用性及应用效果,规范游戏实施的具体流程。展开更多
基金Project supported by the Ministry of Agriculture of China(Nos. 2011ZX08004 and 2009ZX08010013)the National Natural Science Foundation of China(No.31172024)
文摘The Agrobacterium-mediated transformation system is the most commonly used method in soybean transformation.Screening of soybean genotypes favorable for Agrobacterium-infection and tissue regeneration is the most important step to establish an efficient genetic transformation system.In this study,twenty soybean genotypes that originated from different soybean production regions in China were screened for transient infection,regeneration capacity,and stable transgenic efficiency.Three genotypes,Yuechun 04-5,Yuechun 03-3,and Tianlong 1,showed comparable stable transgenic efficiencies with that of the previously reported American genotypes Williams 82 and Jack in our experimental system.For the Tianlong 1,the average stable transformation efficiency is 4.59%,higher than that of control genotypes(Jack and Williams 82),which is enough for further genomic research and genetic engineering.While polymerase chain reaction(PCR),LibertyLink strips,and β-glucuronidase(GUS) staining assays were used to detect the insertion and expression of the transgene,leaves painted with 135 mg/L Basta could efficiently identify the transformants.
文摘目的 系统分析纸牌游戏在预立医疗照护计划中的应用研究,旨在为相关研究及其临床推广应用提供参考。方法 按照范围综述的研究方法,计算机检索中国知网、万方数据库、中国生物医学文献数据库、Cochrane Library、Embase、Medline、Web of Science、PubMed,检索时限为建库至2021年10月2日。收集纸牌游戏应用于预立医疗照护计划中的相关研究。结果 共纳入11篇文献,包括4种纸牌游戏,即My gift of grace纸牌游戏、Heart to Heart纸牌游戏、The Life Unlocking纸牌游戏和Go Wish纸牌游戏。其在促进临终讨论、确定临终偏好、提高预立医疗照护计划相关行为的参与度等方面显示出积极作用。结论 纸牌游戏在预立医疗照护计划中显示出积极的应用效果和可行性,今后还需要多中心、大样本、高质量研究进一步明确其在我国的适用性及应用效果,规范游戏实施的具体流程。
