BACKGROUND: Stone recurrence is a major problem in the medication of gallstones with gallbladder preservation. The aim of this study was to determine the long-term recurrence rate of gallstones and the clinical outcom...BACKGROUND: Stone recurrence is a major problem in the medication of gallstones with gallbladder preservation. The aim of this study was to determine the long-term recurrence rate of gallstones and the clinical outcome after successful percutaneous cholecystolithotomy (PCCL) treatment, and to investigate the possible risk factors for gallstone recurrence. METHODS: After successful PCCL for gallstones, 439 patients were followed up during a 10-year period. The long-term gallstone recurrence rate and clinical outcome were evaluated. Risk factors associated with stone recurrence were identified. RESULTS: Gallstone recurrence was detected in 182 of 439 PCCL patients, giving an overall recurrence rate of 41.46%. The cumulative gallstone recurrence rate for each of the 10 post-operative years was 9.57%, 18.91%, 27.33%, 34.14%, 37.59%, 39.86%, 41.90%, 42.73%, 42.85%, and 43.21%, respectively. Among these recurrent patients, 94 were asymptomatic, 80 suffered from nonspecific upper gastrointestinal symptoms and 8 suffered from abdominal pain or biliary colic. Thirty-eight of the 182 patients were retreated with cholecystectomy. The risk factors for stone recurrence included a family history of gallstones, preference for fatty food, accompanying liver disease, multiple stones and poor gallbladder function pre-PCCL. CONCLUSIONS: In this study, the overall recurrence rate of gallstone was 41.46% during a 10-year period. The highest frequency of gallstone recurrence was during the 5th to 6th postoperative years and then continued to slowly increase. Risk factors for stone recurrence varied. We suggest that the use of PCCL in patients with gallstones should be considered carefully because of stone recurrence.展开更多
Background Macrophage activation syndrome(MAS)is a major cause of morbidity and mortality in pediatric rheumatology.We aimed to further understand the clinical features,treatment,and outcome of MAS in China.Methods A ...Background Macrophage activation syndrome(MAS)is a major cause of morbidity and mortality in pediatric rheumatology.We aimed to further understand the clinical features,treatment,and outcome of MAS in China.Methods A multi-center cohort study was performed in seven hospitals in China from 2012 to 2018.Eighty patients with MAS were enrolled,including 53 cases with systemic juvenile idiopathic arthritis(SJIA-MAS),10 cases of Kawasaki disease(KD-MAS),and 17 cases of connective tissue disease(CTD-MAS).The clinical and laboratory data were collected before(pre-),at onset,and during full-blown stages of MAS.We compared the data among the SJIA-MAS,KD-MAS,and CTD-MAS subjects.Results 51.2%of patients developed MAS when the underlying disease was first diagnosed.In patients with SJIA,22.6%(12/53)were found to have hypotension before the onset of SJIA-MAS.These patients were also found to have significantly increased aspartate aminotransferase(AST)and lactate dehydrogenase(LDH),as well as decreased albumin(P<0.05),but no difference in alanine aminotransferase,ferdtin,and ratio of ferritin/erythrocyte sedimentation rate(ESR)at onset of MAS when compared to pre-MAS stages of the disease.In addition,ferritin and ratio of ferritin/ESR were significantly elevated in patients at full-blown stages of SJIA-MAS compared to pre-MAS stage.Significantly increased ferritin and ratio of ferritin/ESR were also observed in patients with SJIA compared to in KD and CTD.Receiver-operating characteristic analysis showed that 12,217.5μg/L of ferritin and 267.5 of ferritin/ESR ratio had sensitivity(80.0%and 90.5%)and specificity(88.2%and 86.7%),respectively,for predicting full-blown SJIA-MAS.The majority of the patients received corticosteroids(79/80),while biologic agents were used in 12.5%(10/80)of cases.Tocilizumab was the most commonly selected biologic agent.The overall mortality rate was 7.5%.Conclusions About half of MAS occurred when the underlying autoimmune diseases(SJIA,KD,and CTD)were first diagnosed.Hypotension could be an importa展开更多
DMRT基因家族是一个与性别决定有关的基因家族,其编码的转录因子DMRTs蛋白(除DMRT8外)均具有一个DM(double-sex and mab-3)结构域,并通过该结构域与DNA结合而发挥调控作用。目前研究表明,DMRT基因家族在性器官发育(如性别分化、性腺发...DMRT基因家族是一个与性别决定有关的基因家族,其编码的转录因子DMRTs蛋白(除DMRT8外)均具有一个DM(double-sex and mab-3)结构域,并通过该结构域与DNA结合而发挥调控作用。目前研究表明,DMRT基因家族在性器官发育(如性别分化、性腺发育、生殖细胞发育等)与性外器官发育(如肌肉软骨发育、神经系统发育等)中均具有重要作用;同时也有学者提出DMRTs可能参与肿瘤(如前列腺癌、乳腺癌、肺癌等)的发生和发展。本文综述了以人与小鼠为主的哺乳动物DMRTs/Dmrts的结构、在性器官发育与性外器官发育中的重要功能及其与肿瘤的关系的研究进展,提示DMRT基因家族在多器官发育及多种肿瘤发生和发展及治疗等过程中可能发挥作用,能够作为深入研究肿瘤发病机制与治疗策略的候选基因。展开更多
OBJECTIVE To investigate the anit-inflammatory effect of extracts of Dendropanax dentiger(Harms)Merr and Lycopodiastrum casuarinoides(Spring)Holub on rheumatoid arthritis(RA)using adjuvant arthritis(AA)rat model and p...OBJECTIVE To investigate the anit-inflammatory effect of extracts of Dendropanax dentiger(Harms)Merr and Lycopodiastrum casuarinoides(Spring)Holub on rheumatoid arthritis(RA)using adjuvant arthritis(AA)rat model and possible mechanisms.METHODS The AA rat model of RA was induced in adult SparagueDawley(SD)rats by injecting of the adjuvant at base oftail.One-week-old male SD rats were randomly divided into the following groups:normal saline group(blank control),D.dentiger decoction group(80g·kg-1·d-1),L.Casuarinoides decoction group(80 g·kg·d-1),the total of glucoside Tripterygium(GTT)group(positive control,2 mg·kg-1·d-1).They were administered orally for 6weeks.Histopathology of tissues arthritis rats was observed by H.E staining.The volume of paw swelling was measured and the arthritis inflammation index was calculated.The expressions of tumor necrosis factor-α(TNF-α)and interlukin-1β(IL-1β)were detected by the ELISA assay.In addition,previous study has reported that plant-derived mi RNAs play a role for cross kingdom regulatory potential.Thus,we also performed RNA-seq technique to identify bioactive mi RNAs via comparative transcriptome analysis between D.dentiger and L.Casuarinoides.RESULTS Comparing with AA model group,the volume of paw swelling and the arthritis index were increased significantly in the AA rat model group(P<0.01),suggesting that the AA model rats were prepared properly.Compared with the AA model group,the volume of paw swelling of D.dentiger decoction group,L.Casuarinoides decoction group was decreased by 25.2%and 10.3%,respectively,and the arthritis index was decreased by 27.2%and 18.3%,respectively.Compared with AA model group,TNF-αprotein expression of D.dentiger decoction group and L.Casuarinoides decoction groups were decreased by 16.3%and 14.7%,and IL-1βprotein expression was decreased by 23.6%,18.9%(P<0.05,P<0.01),respectively.Besides,we found that some plant-derived homologous mi RNAs(such as mi RNA192 and mi RNA30a)associated with cell apoptosis processing have been scree展开更多
A miniaturized multi-frequency circularly polarized array is designed in this paper.The antenna array is composed of three independent sub-arrays employing modified quarter-mode substrate ntegrated aveguide(QMSIW)to a...A miniaturized multi-frequency circularly polarized array is designed in this paper.The antenna array is composed of three independent sub-arrays employing modified quarter-mode substrate ntegrated aveguide(QMSIW)to achieve three circularly polarized frequency bands.By introducing strip-slot,the impedance bandwidth of the antenna array is broadened while the dimension is decreased by 75%to realize miniaturization.Meanwhile,metasurface causes the impedance bandwidth of the sub-array to be further enhanced.Moreover,the metal vias are employed in the antenna array design to further achieve miniaturization.The antenna array is manufactured and measured to verify the design.Both the measured and simulated results display that the array achieves the impedance bandwidths of 10%,11.7%,and 14.8%and axial ratio bandwidths of 8.8%,8.0%,and 8.5%at 2.5,3.5,and 4.8 GHz,respectively.The gain is stable in the operating band within an uncertainty of 0.7 d Bi.The whole dimension is 0.92λ×0.63λ×0.04λ,whereλ_(0) is the wavelength at the lowest resonant frequency.Furthermore,the simple structure and miniaturization provides great convenience in sub-6 applications.展开更多
文摘BACKGROUND: Stone recurrence is a major problem in the medication of gallstones with gallbladder preservation. The aim of this study was to determine the long-term recurrence rate of gallstones and the clinical outcome after successful percutaneous cholecystolithotomy (PCCL) treatment, and to investigate the possible risk factors for gallstone recurrence. METHODS: After successful PCCL for gallstones, 439 patients were followed up during a 10-year period. The long-term gallstone recurrence rate and clinical outcome were evaluated. Risk factors associated with stone recurrence were identified. RESULTS: Gallstone recurrence was detected in 182 of 439 PCCL patients, giving an overall recurrence rate of 41.46%. The cumulative gallstone recurrence rate for each of the 10 post-operative years was 9.57%, 18.91%, 27.33%, 34.14%, 37.59%, 39.86%, 41.90%, 42.73%, 42.85%, and 43.21%, respectively. Among these recurrent patients, 94 were asymptomatic, 80 suffered from nonspecific upper gastrointestinal symptoms and 8 suffered from abdominal pain or biliary colic. Thirty-eight of the 182 patients were retreated with cholecystectomy. The risk factors for stone recurrence included a family history of gallstones, preference for fatty food, accompanying liver disease, multiple stones and poor gallbladder function pre-PCCL. CONCLUSIONS: In this study, the overall recurrence rate of gallstone was 41.46% during a 10-year period. The highest frequency of gallstone recurrence was during the 5th to 6th postoperative years and then continued to slowly increase. Risk factors for stone recurrence varied. We suggest that the use of PCCL in patients with gallstones should be considered carefully because of stone recurrence.
基金This study is funded by Zhejiang Basic Public Welfare Research Project(LGF19H100002).
文摘Background Macrophage activation syndrome(MAS)is a major cause of morbidity and mortality in pediatric rheumatology.We aimed to further understand the clinical features,treatment,and outcome of MAS in China.Methods A multi-center cohort study was performed in seven hospitals in China from 2012 to 2018.Eighty patients with MAS were enrolled,including 53 cases with systemic juvenile idiopathic arthritis(SJIA-MAS),10 cases of Kawasaki disease(KD-MAS),and 17 cases of connective tissue disease(CTD-MAS).The clinical and laboratory data were collected before(pre-),at onset,and during full-blown stages of MAS.We compared the data among the SJIA-MAS,KD-MAS,and CTD-MAS subjects.Results 51.2%of patients developed MAS when the underlying disease was first diagnosed.In patients with SJIA,22.6%(12/53)were found to have hypotension before the onset of SJIA-MAS.These patients were also found to have significantly increased aspartate aminotransferase(AST)and lactate dehydrogenase(LDH),as well as decreased albumin(P<0.05),but no difference in alanine aminotransferase,ferdtin,and ratio of ferritin/erythrocyte sedimentation rate(ESR)at onset of MAS when compared to pre-MAS stages of the disease.In addition,ferritin and ratio of ferritin/ESR were significantly elevated in patients at full-blown stages of SJIA-MAS compared to pre-MAS stage.Significantly increased ferritin and ratio of ferritin/ESR were also observed in patients with SJIA compared to in KD and CTD.Receiver-operating characteristic analysis showed that 12,217.5μg/L of ferritin and 267.5 of ferritin/ESR ratio had sensitivity(80.0%and 90.5%)and specificity(88.2%and 86.7%),respectively,for predicting full-blown SJIA-MAS.The majority of the patients received corticosteroids(79/80),while biologic agents were used in 12.5%(10/80)of cases.Tocilizumab was the most commonly selected biologic agent.The overall mortality rate was 7.5%.Conclusions About half of MAS occurred when the underlying autoimmune diseases(SJIA,KD,and CTD)were first diagnosed.Hypotension could be an importa
基金supported by the Science and Technology Department of Henan Province,China(No.222102310186,162102310523)the College Students’Innovation and Entrepreneurship Training Program Projects of Zhengzhou University,China(No.2021cxcy290)。
文摘DMRT基因家族是一个与性别决定有关的基因家族,其编码的转录因子DMRTs蛋白(除DMRT8外)均具有一个DM(double-sex and mab-3)结构域,并通过该结构域与DNA结合而发挥调控作用。目前研究表明,DMRT基因家族在性器官发育(如性别分化、性腺发育、生殖细胞发育等)与性外器官发育(如肌肉软骨发育、神经系统发育等)中均具有重要作用;同时也有学者提出DMRTs可能参与肿瘤(如前列腺癌、乳腺癌、肺癌等)的发生和发展。本文综述了以人与小鼠为主的哺乳动物DMRTs/Dmrts的结构、在性器官发育与性外器官发育中的重要功能及其与肿瘤的关系的研究进展,提示DMRT基因家族在多器官发育及多种肿瘤发生和发展及治疗等过程中可能发挥作用,能够作为深入研究肿瘤发病机制与治疗策略的候选基因。
基金The project supported by Science and Technology External Cooperation Key Foundation of Jiangxi Province(20151BDH80020)
文摘OBJECTIVE To investigate the anit-inflammatory effect of extracts of Dendropanax dentiger(Harms)Merr and Lycopodiastrum casuarinoides(Spring)Holub on rheumatoid arthritis(RA)using adjuvant arthritis(AA)rat model and possible mechanisms.METHODS The AA rat model of RA was induced in adult SparagueDawley(SD)rats by injecting of the adjuvant at base oftail.One-week-old male SD rats were randomly divided into the following groups:normal saline group(blank control),D.dentiger decoction group(80g·kg-1·d-1),L.Casuarinoides decoction group(80 g·kg·d-1),the total of glucoside Tripterygium(GTT)group(positive control,2 mg·kg-1·d-1).They were administered orally for 6weeks.Histopathology of tissues arthritis rats was observed by H.E staining.The volume of paw swelling was measured and the arthritis inflammation index was calculated.The expressions of tumor necrosis factor-α(TNF-α)and interlukin-1β(IL-1β)were detected by the ELISA assay.In addition,previous study has reported that plant-derived mi RNAs play a role for cross kingdom regulatory potential.Thus,we also performed RNA-seq technique to identify bioactive mi RNAs via comparative transcriptome analysis between D.dentiger and L.Casuarinoides.RESULTS Comparing with AA model group,the volume of paw swelling and the arthritis index were increased significantly in the AA rat model group(P<0.01),suggesting that the AA model rats were prepared properly.Compared with the AA model group,the volume of paw swelling of D.dentiger decoction group,L.Casuarinoides decoction group was decreased by 25.2%and 10.3%,respectively,and the arthritis index was decreased by 27.2%and 18.3%,respectively.Compared with AA model group,TNF-αprotein expression of D.dentiger decoction group and L.Casuarinoides decoction groups were decreased by 16.3%and 14.7%,and IL-1βprotein expression was decreased by 23.6%,18.9%(P<0.05,P<0.01),respectively.Besides,we found that some plant-derived homologous mi RNAs(such as mi RNA192 and mi RNA30a)associated with cell apoptosis processing have been scree
基金Project supported by the National Natural Science Foundation of China(Grant No.61871394)。
文摘A miniaturized multi-frequency circularly polarized array is designed in this paper.The antenna array is composed of three independent sub-arrays employing modified quarter-mode substrate ntegrated aveguide(QMSIW)to achieve three circularly polarized frequency bands.By introducing strip-slot,the impedance bandwidth of the antenna array is broadened while the dimension is decreased by 75%to realize miniaturization.Meanwhile,metasurface causes the impedance bandwidth of the sub-array to be further enhanced.Moreover,the metal vias are employed in the antenna array design to further achieve miniaturization.The antenna array is manufactured and measured to verify the design.Both the measured and simulated results display that the array achieves the impedance bandwidths of 10%,11.7%,and 14.8%and axial ratio bandwidths of 8.8%,8.0%,and 8.5%at 2.5,3.5,and 4.8 GHz,respectively.The gain is stable in the operating band within an uncertainty of 0.7 d Bi.The whole dimension is 0.92λ×0.63λ×0.04λ,whereλ_(0) is the wavelength at the lowest resonant frequency.Furthermore,the simple structure and miniaturization provides great convenience in sub-6 applications.
