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雷公藤内酯醇联合多西紫杉醇对PC-3/MDR细胞耐药的体外逆转作用 被引量:3
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作者 杨瑜 陈明 +1 位作者 朱伟东 张光远 《中成药》 CAS CSCD 北大核心 2018年第12期2629-2635,共7页
目的研究雷公藤内酯醇联合多西紫杉醇对人前列腺癌PC-3/多药耐药(MDR)细胞耐药的体外逆转作用。方法将对数生长期的PC-3、PC-3/MDR细胞随机分为阴性对照组、多西紫杉醇组、雷公藤内酯醇组、多西紫杉醇-雷公藤内酯醇组(1∶1、1∶2、1∶3)... 目的研究雷公藤内酯醇联合多西紫杉醇对人前列腺癌PC-3/多药耐药(MDR)细胞耐药的体外逆转作用。方法将对数生长期的PC-3、PC-3/MDR细胞随机分为阴性对照组、多西紫杉醇组、雷公藤内酯醇组、多西紫杉醇-雷公藤内酯醇组(1∶1、1∶2、1∶3),MTT法测定各组细胞生长抑制率,HPLC法检测各组细胞对多西紫杉醇的摄取,试剂盒法测定给药组细胞P-gp表达和P-gp ATP酶活性,RT-PCR法考察雷公藤内酯醇干预对PC-3/MDR细胞MDR1 mRNA表达影响。结果不同浓度雷公藤内酯醇联合多西紫杉醇与PC-3、PC-3/MDR细胞孵育后,IC50值均显著降低,其中3倍量前者对后者的耐药逆转指数为9. 75。联合用药组细胞所摄取的多西紫杉醇量显著高于多西紫杉醇组,并呈雷公藤内酯醇剂量依赖性。此外,高浓度雷公藤内酯醇对MDR1 mRNA表达有明显抑制作用。结论雷公藤内酯醇可通过抑制P-gp表达、激活P-gp ATP酶活性、抑制MDR1 mRNA表达等多重机制,促使多西紫杉醇在体外逆转前列腺肿瘤PC-3/MDR细胞耐药。 展开更多
关键词 雷公藤内酯醇 多西紫杉醇 PC-3细胞 多药耐药(MDR)
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Dynamics of Gradient Bioceramic Composite Coating on Surface of Titanium Alloy by Wide-Band Laser Cladding
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作者 LIUQi-bin ZOULong-jiang +2 位作者 zhuwei-dong LIHai-tao dongChuang 《Journal of Iron and Steel Research International》 SCIE EI CAS CSCD 2004年第6期57-62,共6页
The gradient bioceramic coating was prepared on the surface of titanium alloy using wide-band laser cladding.The dynamics of gradient bioceramic composite coating containing hydroxyapatite(HA)prepared with mixture of ... The gradient bioceramic coating was prepared on the surface of titanium alloy using wide-band laser cladding.The dynamics of gradient bioceramic composite coating containing hydroxyapatite(HA)prepared with mixture of CaHPO4·2H2O and CaCO3 under the condition of wide-band laser was studied theoretically.The corresponding mathematical model and its numerical solution were presented.The examination experiment showed that HA bioceramic composite coatings can be obtained by appropriately choosing wide-band laser cladding parameters.The microstructure and surface morphology of HA bioceramic coating were observed by SEM and X-ray diffraction.The experimental results showed that the bioceramic coating is composed of HA,β-TCP,CaO,CaTiO3 and TiO2.The surface of bioceramic coating takes coral-shaped structure or short-rod piled structure,which helps osteoblast grow into bioceramic and improves the biocompatibility. 展开更多
关键词 wide-band laser cladding BIOCERAMIC composite coating DYNAMICS
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