Two G. somalense monosomic alien addition lines were identified from the derived backcross progenies of allohexaploid between G. hirsutum and G. somalense through cytological and morphological observation. Furthermore...Two G. somalense monosomic alien addition lines were identified from the derived backcross progenies of allohexaploid between G. hirsutum and G. somalense through cytological and morphological observation. Furthermore, the alien addition chromosome was identified and distinguished by RAPD analysis. A total of 160 RAPD primers were used for PCR amplification. Primer SBSG11 could produce a spe-cific molecular marker (600 bp) for monosomic alien addi-tion line Ⅰ(MAAL Ⅰ). Primer SBSC03 could produce a specific molecular marker (700 bp) for monosomic alien ad-dition line Ⅱ(MAAL Ⅱ). SBSE07 and SBSE08 could re-spectively produce common molecular marker for mono-somic alien addition lines Ⅰ and Ⅱ. G . somalense alien addition lines could be important for cotton improvement.展开更多
文摘Two G. somalense monosomic alien addition lines were identified from the derived backcross progenies of allohexaploid between G. hirsutum and G. somalense through cytological and morphological observation. Furthermore, the alien addition chromosome was identified and distinguished by RAPD analysis. A total of 160 RAPD primers were used for PCR amplification. Primer SBSG11 could produce a spe-cific molecular marker (600 bp) for monosomic alien addi-tion line Ⅰ(MAAL Ⅰ). Primer SBSC03 could produce a specific molecular marker (700 bp) for monosomic alien ad-dition line Ⅱ(MAAL Ⅱ). SBSE07 and SBSE08 could re-spectively produce common molecular marker for mono-somic alien addition lines Ⅰ and Ⅱ. G . somalense alien addition lines could be important for cotton improvement.
