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硅胶固相萃取净化/高效液相色谱-串联质谱法测定海洋生物体中六溴环十二烷 被引量:4
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作者 严忠雍 张小军 +3 位作者 陈雪昌 方益 张帅 孙秀梅 《分析测试学报》 CAS CSCD 北大核心 2018年第6期714-718,共5页
建立了海洋生物体中六溴环十二烷(HBCDs)的硅胶固相萃取净化/液相色谱-串联质谱法。样品采用正己烷提取,经硅胶固相萃取柱净化,液相色谱-串联质谱分析。根据硅胶固相萃取柱的使用特性,对洗脱条件进行优化。在ACQUITY UPLC BEH C18色谱... 建立了海洋生物体中六溴环十二烷(HBCDs)的硅胶固相萃取净化/液相色谱-串联质谱法。样品采用正己烷提取,经硅胶固相萃取柱净化,液相色谱-串联质谱分析。根据硅胶固相萃取柱的使用特性,对洗脱条件进行优化。在ACQUITY UPLC BEH C18色谱柱上进行分离,流动相为水和甲醇乙腈(V甲醇∶V乙腈=4∶6)混合溶液,梯度洗脱。质谱采用电喷雾负离子电离,多反应监测模式,内标法定量。六溴环十二烷在0.50~100.0μg/L范围内线性关系良好,相关系数(r2)大于0.995,定量下限为0.20μg/kg,回收率为85.6%~97.3%,相对标准偏差为3.9%~8.8%。该方法基质干扰小、重复性好,适用于海洋生物中六溴环十二烷的测定。 展开更多
关键词 固相萃取(SPE) 液相色谱-串联质谱 海洋生物体 六溴环十二烷(HBCDs)
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STUDY ON ECOLOGICAL SPACE OF URBAN AGGLOMERATION-Taking Wuxi City as an Example 被引量:4
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作者 XUEDong-qian zhangxiao-jun 《Chinese Geographical Science》 SCIE CSCD 2002年第4期321-328,共8页
From the angle of ecology,urban agg lomeration presents relevant types of landscape structure,which in-clude Patch,Corridor and Matrix.There are different landscape feature s and different problems in differen t devel... From the angle of ecology,urban agg lomeration presents relevant types of landscape structure,which in-clude Patch,Corridor and Matrix.There are different landscape feature s and different problems in differen t development phases.This paper has put forward fi ve basic conditions for security pattern of landscape ecology of urban agglomeration,described quantitatively the featu res for landscape ecology in Wuxi,an d analyzed ecological background of spatial expan-sion and spatial organization of urb an development in Wuxi.From the angle of ecological land use and non-ecological land use,the paper has analyzed the featu res of land use and ecological distribution of land in the urban area.The s patial mod-el of cities and towns in Wuxi is composed of one metropolis,two urban zones and three development axes.This th esis has planned preliminarily ecologic al protection network at four levels in the urban region according to four layers.At last,combining landscape ecology with ur ban space,a tentative security pattern of landscape ecology has been pla nned in Wux-i,namely Source-Buffer Zone and Metropolis,Radiating Routes and Expan sion Direction of City,Strategic Point and Interac-tion between Cities and Towns,Inter-Source Linkage-Corridor of a Stabl e Landscape Structure. 展开更多
关键词 urban agglomeration spatial structure landscape structure securitypattern of landscape ecology
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Isolation and characterization of a new species of Vibrio-Vibrio olivaceus sp.nov. from diseased flounder (Paralichthys olivaceus temminck et schlegel)
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作者 FangHai zhangxiao-jun +2 位作者 ChenCuizhen ZhanWenbin JinXiaomin 《High Technology Letters》 EI CAS 2005年第1期107-112,共6页
One kind of pathogenic bacteria was isolated from diseased (or dead) flounder (Paralichthys olivaceus L.) which expressed bacterial septicaemia. The phenotyic information of the 10 pure cultures was studied extensivel... One kind of pathogenic bacteria was isolated from diseased (or dead) flounder (Paralichthys olivaceus L.) which expressed bacterial septicaemia. The phenotyic information of the 10 pure cultures was studied extensively, including morphological characteristics, colony characteristics in different media, physiological and biochemical characteristics, and the mol%G+C ratio of the DNA for representative strain. The results showed that the isolates belonged to a new species of Vibrio. In addition, the representative strains have been re-checked by China Center for Type Culture Collection (CCTCC), the results were the same as ours, the examined strains were also regarded as a new species of Vibrio, and designated as Vibrio olivaceus sp.nov. by its isolated fish (Paralichthys olivaceus) based on its biological properties following Rules of International Code of Nomenclature of Bacteria. In the same time, we conducted studies on the serum homology of isolates, pathogenicity of isolates by experimental infection. The results showed that all the isolates were of serologic similarity and the isolates have strong pathogenicity to flounder. Inspection to pure cultures and isolates from liver of moribund fish from infection experiment by fluorescent antibody technique showed the feasibility of fluorescent antibody technique as an auxiliary method of examination. 展开更多
关键词 比目鱼 鱼病 生物特征 弧菌 微生物 病原体
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小切口拨核技术在超声乳化术中的应用
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作者 刘为友 钱素珍 张晓俊 《实用防盲技术》 2021年第4期159-161,共3页
目的探讨拨核技术在超声乳化手术中的应用效果。方法对兴化楚水医院2018年3~10月开展的晶状体超声乳化手术78例(78眼)进行回顾性研究,术中应用小切口拨核方法,先将核松动游离半脱位于前房,进一步旋拨核,令核完全松动,再以黏弹剂压回囊袋... 目的探讨拨核技术在超声乳化手术中的应用效果。方法对兴化楚水医院2018年3~10月开展的晶状体超声乳化手术78例(78眼)进行回顾性研究,术中应用小切口拨核方法,先将核松动游离半脱位于前房,进一步旋拨核,令核完全松动,再以黏弹剂压回囊袋内,行超声劈核,观察手术效果。结果 49眼(62.82%)一次劈核将核分成2块,19眼(24.36%)核未能一次完全分开,10眼(12.82%)超声过程中形成核碗。2眼发生后囊破裂。结论应用拨核技术将核完全松动游离再行超声劈核,是一种安全有效的方法。 展开更多
关键词 小切口 拨核技术 超声乳化
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