Floor water inrush is one of the main types of coal mine water hazards.With the development of deep mining,the prediction and evaluation of floor water inrush is particularly significant.This paper proposes a variable...Floor water inrush is one of the main types of coal mine water hazards.With the development of deep mining,the prediction and evaluation of floor water inrush is particularly significant.This paper proposes a variable weight model,which combines a multi-factor interaction matrix(MFIM)and the technique for order performance by similarity to ideal solution(TOPSIS)to implement the risk assessment of floor water inrush in coal mines.Based on the MFIM,the interaction between seven evaluation indices,including the confined water pressure,water supply condition and aquifer water yield property,floor aquifuge thickness,fault water transmitting ability,fracture development degree,mining depth and thickness and their influence on floor water inrush were considered.After calculating the constant weights,the active degree evaluation was used to assign a variable weight to the indices.The values of the middle layer and final risk level were obtained by TOPSIS.The presented model was successfully applied in the 9901 working face in the Taoyang Mine and four additional coal mines and the results were highly consistent with the engineering situations.Compared with the existing nonlinear evaluation methods,the proposed model had advantages in terms of the weighting,principle explanation,and algorithm structure.展开更多
目的检测小鼠脑挫伤后不同时间点大麻素2型受体(cannabinoid type 2 receptor,CB2R)的表达情况,并探索其与损伤时间的相关性。方法通过PCI3000精密颅脑损伤撞击器建立小鼠脑挫伤模型,利用免疫组织化学染色、免疫荧光染色和Western印迹...目的检测小鼠脑挫伤后不同时间点大麻素2型受体(cannabinoid type 2 receptor,CB2R)的表达情况,并探索其与损伤时间的相关性。方法通过PCI3000精密颅脑损伤撞击器建立小鼠脑挫伤模型,利用免疫组织化学染色、免疫荧光染色和Western印迹法检测不同损伤时间点损伤区周围CB2R的表达变化。结果免疫组织化学染色结果显示,假手术组脑皮质中仅少量细胞呈CB2R阳性表达,脑挫伤后CB2R阳性细胞率逐渐升高,于伤后12h和伤后7d两次达到高峰,随后逐渐下降,至伤后28d基本恢复至正常水平。Western印迹法检测结果与免疫组织化学染色结果一致。免疫荧光染色结果显示,脑挫伤后CB2R阳性神经元、CB2R阳性单核细胞、CB2R阳性星形胶质细胞数与总细胞数的比值变化均呈单峰模式,分别于伤后12h、1d、7d达到高峰。结论小鼠脑挫伤后CB2R在神经元、单核细胞及星形胶质细胞中的表达提示,其可能参与调节这些细胞的生物学功能。脑挫伤后CB2R的动态表达存在时间规律性,有望成为法医学脑挫伤损伤时间推断的生物学指标。展开更多
基金Projects(41877239,51379112,51422904,40902084,41772298)supported by the National Natural Science Foundation of ChinaProject(2019GSF111028)supported by the Key Technology Research and Development Program of Shandong Province,China+1 种基金Project(2018JC044)supported by the Fundamental Research Funds of Shandong University,ChinaProject(JQ201513)supported by the Natural Science Foundation of Shandong Province,China。
文摘Floor water inrush is one of the main types of coal mine water hazards.With the development of deep mining,the prediction and evaluation of floor water inrush is particularly significant.This paper proposes a variable weight model,which combines a multi-factor interaction matrix(MFIM)and the technique for order performance by similarity to ideal solution(TOPSIS)to implement the risk assessment of floor water inrush in coal mines.Based on the MFIM,the interaction between seven evaluation indices,including the confined water pressure,water supply condition and aquifer water yield property,floor aquifuge thickness,fault water transmitting ability,fracture development degree,mining depth and thickness and their influence on floor water inrush were considered.After calculating the constant weights,the active degree evaluation was used to assign a variable weight to the indices.The values of the middle layer and final risk level were obtained by TOPSIS.The presented model was successfully applied in the 9901 working face in the Taoyang Mine and four additional coal mines and the results were highly consistent with the engineering situations.Compared with the existing nonlinear evaluation methods,the proposed model had advantages in terms of the weighting,principle explanation,and algorithm structure.
文摘目的检测小鼠脑挫伤后不同时间点大麻素2型受体(cannabinoid type 2 receptor,CB2R)的表达情况,并探索其与损伤时间的相关性。方法通过PCI3000精密颅脑损伤撞击器建立小鼠脑挫伤模型,利用免疫组织化学染色、免疫荧光染色和Western印迹法检测不同损伤时间点损伤区周围CB2R的表达变化。结果免疫组织化学染色结果显示,假手术组脑皮质中仅少量细胞呈CB2R阳性表达,脑挫伤后CB2R阳性细胞率逐渐升高,于伤后12h和伤后7d两次达到高峰,随后逐渐下降,至伤后28d基本恢复至正常水平。Western印迹法检测结果与免疫组织化学染色结果一致。免疫荧光染色结果显示,脑挫伤后CB2R阳性神经元、CB2R阳性单核细胞、CB2R阳性星形胶质细胞数与总细胞数的比值变化均呈单峰模式,分别于伤后12h、1d、7d达到高峰。结论小鼠脑挫伤后CB2R在神经元、单核细胞及星形胶质细胞中的表达提示,其可能参与调节这些细胞的生物学功能。脑挫伤后CB2R的动态表达存在时间规律性,有望成为法医学脑挫伤损伤时间推断的生物学指标。
