A new ion chromatography coupled with tandem mass spectrometry （IC-ESI-MS/MS） method, with automated sampling and on-line preconcentration, has been developed for the determination of perchlorate in Antarctic snow a...A new ion chromatography coupled with tandem mass spectrometry （IC-ESI-MS/MS） method, with automated sampling and on-line preconcentration, has been developed for the determination of perchlorate in Antarctic snow and ice at low part-per-trillion （ng]L） levels. To the best of our lmowledge, this is the first time that an analytical method is used for the determination of perchlorate in Antarctic snow and ice. The IC-ESI-MS/MS instrumentation consisted of an ICS2000 ion chromatography （IC） system coupled to an API3200 electrospray tandem mass spectrometer （ESI-MS/MS）. On-line preconcentration was realized through a six-port injector valve, a TAC-ULP1 concentrator column and an AS auto-sampler. Multiple reaction monitoring （MRM） mode was used to quantify the perch/orate anion. The transition of 35Cl]604- （m/z 98.9） into 3SC11603 （m[z 82.9） was monitored for quantifying the main analyte, and the transition of 37C11604 （m/z 100.9） into 37C1~603- （m]z 84.9） was monitored for examining a proper isotopic abundance ratio of 3sCl to 37C1, which was used as a confirmation tool. The limit of detection （LOD） and limit of quantitation （LOQ） for the method was 0.2 ng/ L and 0.5 ng/L, respectively. And this new method exhibited acceptable accuracy and precision for samples at ng/L levels. All the tested snow and ice samples were found to contain measurable amount of oerchlorate, ran^in~ from 10 nell to 340 nell..展开更多
Background： The efficacy and safety of telmisartan combined with clopidogrel, leflunomide, or both drugs for immunoglobulin A nephropathy （IgAN） are unclear. This study was designed to evaluate the efficacy and saf...Background： The efficacy and safety of telmisartan combined with clopidogrel, leflunomide, or both drugs for immunoglobulin A nephropathy （IgAN） are unclear. This study was designed to evaluate the efficacy and safety of telmisartan combined with clopidogrel, leflunomide, or both drugs for IgAN. Methods： It is a multicenter, prospective, double-dummy randomized controlled trial. Primary IgAN patients were recruited in 13 renal units across Beijing, China, from July 2010 to June 2012. After a 4-week telmisartan （80 mg/d） wash-in, 400 patients continuing on 80 mg/d telmisartan were randomly assigned to additionally receive placebo （Group A）, 50 mg/d clopidogrel （Group B）, 20 mg/d leflunomide （Group C）, or 50 mg/d clopidogrel and 20 mg/d leflunomide （Group D）. The 24-week intervention was completed by 360 patients. The primary endpoint was change in 24-h proteinuria at 24 weeks. A linear mixed-effect model was used to analyze the changes at 4, 12, and 24 weeks. Generalized estimating equations were used to evaluate changes in hematuria grade. This trial was registered at the Chinese Clinical Trial Registry. Results： The effects oftelmisartan combined with leflunomide on changes in proteinuria （0.36 [95% confidence interval （CI） 0.18 0.55] g/d, P 〈 0.001）, in serum uric acid （76.96 [95% CI 57.44-96.49] μmol/L, P 〈 0.001）, in serum creatinine （9.49 [95% CI 6.54-12.44]μmol/L, P 〈 0.001）, and in estimated glomerular filtration rate （-6.72 [95% CI-9.46 to -3.98] ml.min -1. 1.73 m -2, p 〈 0.001） were statistically significant, whereas they were not statistically significant on changes in systolic and diastolic blood pressure and weight （P 〉 0.05）.Telmisartan combined with clopidogrel had no statistical effect on any outcome, and there was no interaction between the interventions. No obvious adverse reactions were observed. Conclusions： Telmisartan combined with leflunomide, not clopidogrel, is safe and effective for decreasing proteinuria in certain IgAN 展开更多
Background Cisplatin （DDP） is one of most effective and most commonly used therapeutic agent in treating tumors,it can accumulate in the kidney and lead to acute renal failure.MicroRNA-181a can induce cell apoptosis...Background Cisplatin （DDP） is one of most effective and most commonly used therapeutic agent in treating tumors,it can accumulate in the kidney and lead to acute renal failure.MicroRNA-181a can induce cell apoptosis by suppressing the expression of Bcl-2 family.In the present study,we investigated the role of microRNA-181a in the apoptosis of tubular epithelial cell induced by DDP.Methods HK-2 cells were cultured,transfected with microRNA-181a inhibitor for 48 hours,and stimulated with 50 μmol/L cisplatin for 24 hours.MicroRNA-181a expression was analyzed by real time PCR,and cell apoptosis was detected by flow cytometry.Moreover,Bcl-2 and Bcl-2-associated X protein （Bax） expression were measured by Western blotting.Results MicroRNA-181a expression significantly down-regulated in cells transfected with microRNA-181a inhibitor,compared with that in untransfectd cells （21.19±2.01 vs.38.87±1.97,P 〈0.05）.Cell apoptosis induced by DDP significantly decreased in cells transfected with MicroRNA-181a inhibitor.Compared with DDP treated cells alone,Bcl-2 expression strikingly was up-regulated and Bax expression was down-regulated in cells transfected with microRNA-181a inhibitor.Conclusion One pathway of DDP induces apoptosis of tubular epithelial cell by suppressing Bcl-2 expression is achieved by regulating the target gene of MicroRNA-181a.展开更多
7075 aluminum alloy was used to obtain elbow parts by complex thixoextrusion and the microstructure evolution and mechanical properties during this process were studied by SEM,TEM and other analytical methods.The resu...7075 aluminum alloy was used to obtain elbow parts by complex thixoextrusion and the microstructure evolution and mechanical properties during this process were studied by SEM,TEM and other analytical methods.The results show that different parts in 7075 aluminum alloy show quite different microstructures.The microstructure of the thin walls formed by back-extrusion is stratified,and the bottom of the parts formed by angular extrusion is obviously deformed.Shear forces contribute to the crushing of grains and the coarse second phase.The main strengthening phases in the material areηphase and E phase,whose amounts greatly decrease during heating to semi-solid and thixoextrusion.After heat treatment,they can precipitate evenly to improve the mechanical properties of the material.The average tensile strength of whole part after thixoextrusion and heat treatment is 485.49 MPa,while the average elongation is 5.49%.展开更多
The microstructure, composition and shape of precipitated phase under as-cast and finished product state of 5Cr21Mn9Ni4N steel with different rare earth （RE） amount were studied. Mechanical properties of 5Cr21Mn9Ni4...The microstructure, composition and shape of precipitated phase under as-cast and finished product state of 5Cr21Mn9Ni4N steel with different rare earth （RE） amount were studied. Mechanical properties of 5Cr21Mn9Ni4N steels withont RE addition and with RE added by 0. 2% in mass percent were tested respectively. The results indicate that the solid solution amount of RE is about 10^-6 -10^-5 order of magnitude in 5Cr21Mn9Ni4N steel. Dendrite of as-cast condition is refined obviously and dimension of interstitial phase is shortened when RE is added by 0.10%-0.20%. But the microstructure will be coarser if surplus RE is added. Precipitated phase under finished product state distributes evenly in nearly same size with RE added by 0. 2% which leads to a largely improved high temperature mechanical property.展开更多
Glaucoma, a type of optic neuropathy, is characterized by the loss of retinal ganglion cells. It remains controversial whether c-Jun N-terminal kinase （JNK） participates in the apoptosis of retinal ganglion cells in...Glaucoma, a type of optic neuropathy, is characterized by the loss of retinal ganglion cells. It remains controversial whether c-Jun N-terminal kinase （JNK） participates in the apoptosis of retinal ganglion cells in glaucoma. This study sought to explore a possible mechanism of action of JNK signaling pathway in glaucoma-induced retinal optic nerve damage. We established a mouse model of chronic ocular hypertension by reducing the aqueous humor followed by pho- tocoagulation using the laser ignition method. Results showed significant pathological changes in the ocular tissues after the injury. Apoptosis of retinal ganglion cells increased with increased intraocular pressure, as did JNK3 mRNA expression in the retina. These data indicated that the increased expression of JNK3 mRNA was strongly associated with the increase in intraocular pressure in the retina, and correlated positively with the apoptosis of retinal ganglion cells.展开更多
Metallic zinc is an excellent anode material for Zn-ion batteries,but the growth of Zn dendrite severely hinders its practical application.Herein,an efficient and economical cationic additive,poly dimethyl diallyl amm...Metallic zinc is an excellent anode material for Zn-ion batteries,but the growth of Zn dendrite severely hinders its practical application.Herein,an efficient and economical cationic additive,poly dimethyl diallyl ammonium(PDDA) was reported,used in aqueous Zn-ion batteries electrolyte for stabilizing Zn anode.The growth of zinc dendrites can be significantly restrained by benefiting from the pronounced electrostatic shielding effect from PDDA on the Zn metal surface.Moreover,the PDDA is preferentially absorbed on Zn(002) plane,thus preventing unwanted side reactions on Zn anode.Owing to the introduction of a certain amount of PDDA additive into the common ZnSO_(4)-based electrolyte,the cycle life of assembled Zn‖Zn cells(1 mA·cm^(-2) and 1 mAh·cm^(-2)) is prolonged to more than 1100 h.In response to the perforation issue of Zn electrodes caused by PDDA additives,the problem can be solved by combining foamy copper with zinc foil.For real application,Zn-ion hybrid supercapacitors and MnO_(2)‖Zn cells were assembled,which exhibited excellent cycling stability with PDDA additives.This work provides a new solution and perspective to cope with the dendrite growth problem of Zn anode.展开更多
BACKGROUND Gelastic seizure(GS)is a rare type of epilepsy that most commonly appears in patients with hypothalamic hamartoma.It is rarely associated with other types of brain damage.This particular type of epilepsy is...BACKGROUND Gelastic seizure(GS)is a rare type of epilepsy that most commonly appears in patients with hypothalamic hamartoma.It is rarely associated with other types of brain damage.This particular type of epilepsy is relatively rare and has few links to other brain lesions.Temporal lobe malacia is mostly caused by cerebral infarction or cerebral hemorrhage,which can lead to seizures.We report a case of GS in a woman with temporal lobe malacia which was reported for the first time in the literature.CASE SUMMARY A 73-year-old female,diagnosed case of GS,presented with repetitive stereotyped laughter a month prior to presentation,happening multiple times daily and with each time lasting for 5-15s.Electroencephalogram displayed a focal seizure seen in the right temporal region.Magnetic resonance imaging head with contrast showed a right temporal lobe malacia.The patient was started on levetiracetam daily.The patient indicated that they had fully recovered and were not experiencing any recurrent or stereotyped laughter during their daily routines.These results remained consistent even after a one-year follow-up period.CONCLUSION GS can be caused by temporal lobe malacia,which is an uncommon but potentially grave condition.The outcome of this present case exhibited the importance of the temporal lobe in the genesis of GS.展开更多
The semi-hydrogenation of alkyne to form Z-olefins with high conversion and high selectivity is still a huge challenge in the chemical industry.Moreover,flammable and explosive hydrogen as the common hydrogen source o...The semi-hydrogenation of alkyne to form Z-olefins with high conversion and high selectivity is still a huge challenge in the chemical industry.Moreover,flammable and explosive hydrogen as the common hydrogen source of this reaction increases the cost and danger of industrial production.Herein,we connect the photocatalytic hydrogen evolution reaction and the semihydrogenation reaction of alkynes in series and successfully realize the high selective production of Z-alkenes using low-cost,safe,and green water as the proton source.Before the cascade reaction,a series of isomorphic metal–organic cage catalysts(Co_(x)Zn_(8−x)L_(6),x=0,3,4,5,and 8)are designed and synthesized to improve the yield of the photocatalytic hydrogen production.Among them,Co_(5)Zn_(3)L_(6) shows the highest photocatalytic activity,with a H_(2) generation rate of 8.81 mmol g^(−1) h^(−1).Then,Co_(5)Zn_(3)L_(6) is further applied in the above tandem reaction to efficiently reduce alkynes to Z-alkenes under ambient conditions,which can reach high conversion of>98%and high selectivity of>99%,and maintain original catalytic activity after multiple cycles.This“one-pot”tandem reaction can achieve a highly selective and safe stepwise conversion from water into hydrogen into Z-olefins under mild reaction conditions.展开更多
The chiral magnetic wave(CMW)is a collective mode in quark-gluon plasma originated from the chiral magnetic effect(CME)and chiral separation effect.Its detection in heavy-ion collisions is challenging owing to signifi...The chiral magnetic wave(CMW)is a collective mode in quark-gluon plasma originated from the chiral magnetic effect(CME)and chiral separation effect.Its detection in heavy-ion collisions is challenging owing to significant background contamination.In[Y.S.Zhao et al.,Phys.Rev.C 106,L051901(2022)],we constructed a neural network that accurately identifies the CME-related signal from the final-state pion spectra.In this study,we have generalized this neural network to the case of CMW search.We show that,after an updated training,the neural network effectively recognizes the CMW-related signal.Additionally,we have assessed the performance of the neural network in comparison with other known methods for CMW search.展开更多
In the era of precision medicine,it has been increasingly recognized that individuals with a certain disease are complex and different from each other.Due to the underestimation of the significant heterogeneity across...In the era of precision medicine,it has been increasingly recognized that individuals with a certain disease are complex and different from each other.Due to the underestimation of the significant heterogeneity across participants in traditional"one-sizefits-all"trials,patient-centered trials that could provide optimal therapy customization to individuals with specific biomarkers were developed including the basket,umbrella,and platform trial designs under the master protocol framework.In recent years,the successive FDA approval of indications based on biomarker-guided master protocol designs has demonstrated that these new clinical trials are ushering in tremendous opportunities.Despite the rapid increase in the number of basket,umbrella,and platform trials,the current clinical and research understanding of these new trial designs,as compared with traditional trial designs,remains limited.The majority of the research focuses on methodologies,and there is a lack of in-depth insight concerning the underlying biological logic of these new clinical trial designs.Therefore,we provide this comprehensive review of the discovery and development of basket,umbrella,and platform trials and their underlying logic from the perspective of precision medicine.Meanwhile,we discuss future directions on the potential development of these new clinical design in view of the"Precision Pro","Dynamic Precision",and"Intelligent Precision".This review would assist trial-related researchers to enhance the innovation and feasibility of clinical trial designs by expounding the underlying logic,which be essential to accelerate the progression of precision medicine.展开更多
The effects of pulsed magnetic field on the solidified microstructure of Mg-Gd-Y-Zr alloy were investigated. Fine uniform equiaxed grains are acquired in the whole ingot by the pulsed magnetic field treatment,and the ...The effects of pulsed magnetic field on the solidified microstructure of Mg-Gd-Y-Zr alloy were investigated. Fine uniform equiaxed grains are acquired in the whole ingot by the pulsed magnetic field treatment,and the average grain size of the as-cast φ50 mm and φ100 mm ingots is refined to 37 μm and 47 μm with the pulsed magnetic field.The macrosegregation of solute elements of Mg-Gd-Y-Zr alloy is also reduced by the pulsed magnetic field treatment. Structure refinement is due to the electromagnetic undercooling zone in front of the solid/liquid interface by the magnetic pressure,and reduction of temperature gradient by the vibration of melt resulted from the pulsed magnetic field,which increases the nucleation rate and prohibits dendrite growth.In addition,primary α-Mg dendrites break into fine crystals, resulting in a refined solidification structure of the magnesium alloys.The Joule heat effect induced in the melt also strengthens the grain refinement effect and the spheroidization of dendrite arms.展开更多
In order to impart electrical conductivity to the magnesium alloy micro-arc oxidation(MAO)coating,the electroless copper plating was performed.Effects of plating temperature and complexing agent concentration on the p...In order to impart electrical conductivity to the magnesium alloy micro-arc oxidation(MAO)coating,the electroless copper plating was performed.Effects of plating temperature and complexing agent concentration on the properties of the electroless copper plating layers were studied by measuring their microstructure,corrosion resistance and electrical conductivity.It was found that the optimized plating temperature was 60°C,and the most suitable value of the complexing agent concentration was 30 g/L.Under this condition,a complete and dense plating layer could be obtained.The formation mechanism of the plating layer on magnesium alloy MAO coating was analyzed.A three-stage model of the plating process was proposed.The square resistance of the plated specimen was finally reduced to 0.03Ω/□after the third stage.Through electroless copper plating,the MAO coated sample obtained excellent electrical conductivity without significantly reducing its corrosion resistance.展开更多
Mg-A1 mixed oxides with different Mg/A1 molar ratio were prepared by thermal decomposition of hydrotalcite- like precursors at 500 ℃ for 5.0 h and used as catalysts for the transesterification of diphenyl carbonate w...Mg-A1 mixed oxides with different Mg/A1 molar ratio were prepared by thermal decomposition of hydrotalcite- like precursors at 500 ℃ for 5.0 h and used as catalysts for the transesterification of diphenyl carbonate with 1,4-butanediol to synthesize high-molecular-weight poly（butylene carbonate） （PBC）. The structure-activity correlations of these catalysts in this transesterification process were discussed by means of various characterization techniques. It was found that the chain growth for the formation of PBC can only be obtained through connecting -OH and -OC（C）OC6H5 end-group upon removing the generated phenol, and the sample with Mg/A1 molar ratio of 4.0 exhibited the best catalytic performance, giving PBC with Mw of 1.64 × 105 g/mol at 210℃ for 3.0 h. This excellent activity depended mainly on the specific surface area and basicity rather than pore structure or crystallite size of MgO.展开更多
Cold stress is an important factor that limits apple production. In this study, we examined the tissue-cultured plantlets of apple rootstocks ‘M9T337' and ‘60-160', which are resistant and sensitive to cold ...Cold stress is an important factor that limits apple production. In this study, we examined the tissue-cultured plantlets of apple rootstocks ‘M9T337' and ‘60-160', which are resistant and sensitive to cold stress, respectively. The enriched pathways of differentially expressed genes(DEGs) and physiological changes in ‘M9T337' and ‘60-160' plantlets were clearly different after cold stress(1°C) treatment for 48 h, suggesting that they have differential responses to cold stress. The differential expression of WRKY transcription factors in the two plantlets showed that MdWRKY40is and MdWRKY48 are potential regulators of cold tolerance. When we overexpressed MdWRKY40is and MdWRKY48in apple calli, the overexpression of MdWRKY48 had no significant effect on the callus, while MdWRKY40is overexpression promoted anthocyanin accumulation, increased callus cold tolerance, and promoted the expression of anthocyanin structural gene MdDFR and cold-signaling core gene MdCBF2. Yeast one-hybrid screening and electrophoretic mobility shift assays showed that MdWRKY40is could only bind to the MdDFR promoter. Yeast twohybrid screening and bimolecular fluorescence complementation showed that MdWRKY40is interacts with the CBF2inhibitor MdMYB15L through the leucine zipper(LZ). When the LZ of MdWRMY40is was knocked out, MdWRKY40is overexpression in the callus did not affect MdCBF2 expression or callus cold tolerance, indicating that MdWRKY40is acts in the cold signaling pathway by interacting with MdMYB15L. In summary, MdWRKY40is can directly bind to the MdDFR promoter in order to promote anthocyanin accumulation, and it can also interact with MdMYB15L to interfere with its inhibitory effect on MdCBF2, indirectly promoting MdCBF2 expression, and thereby improving cold tolerance.These results provide a new perspective for the cold-resistance mechanism of apple rootstocks and a molecular basis for the screening of cold-resistant rootstocks.展开更多
In this paper, we study the multiplicity of positive solutions to the following m-point boundary value problem of nonlinear fractional differential equations： Dqu（t） ＋ f（t, u（t）） = 0, 0 t 1, u（0） = 0, u（1）...In this paper, we study the multiplicity of positive solutions to the following m-point boundary value problem of nonlinear fractional differential equations： Dqu（t） ＋ f（t, u（t）） = 0, 0 t 1, u（0） = 0, u（1） =sum （μiDpu（t）｜t = ξi ） from i =1 to ∞ m-2, where q ∈R , 1 q ≤2 , 0 ξ1 ξ2 ··· ξm-2 ≤ 1/2 , μi ∈[0 , ＋∞） and p = q-1/2 , Γ（q） sum （μiξi（q-1）/2 Γ（（ q＋1）/2） from i =1 to ∞ m-2,Dq is the standard Riemann-Liouville differentiation, and f ∈C （[0 , 1]×[0 , ＋∞） , [0 , ＋∞））. By using the Leggett-Williams fixed point theorem on a convex cone, some multiplicity results of positive solutions are obtained.展开更多
The development of metamorphic petrology to metamorphic geology in China has a long history.Ancient basement metamorphic rocks are distributed primarily in the North China Craton,the Yangtze Block and Tarim Craton.The...The development of metamorphic petrology to metamorphic geology in China has a long history.Ancient basement metamorphic rocks are distributed primarily in the North China Craton,the Yangtze Block and Tarim Craton.They are mainly made up of plutonic gneiss and metamorphosed supercrust rock,transformed to granulite facies through Archean Paleoproterozoic.Many of the Paleoproterozoic metamorphic rocks have undergone high-pressure granulite facies metamorphism with a clockwise metamorphic evolution path.The ultrahigh temperature (UHT)granulites from the Late Paleoproterozoic are found in North China Craton.Many high-precision chronological data have allowed preliminary construction of the formation and evolutionary framework of different metamorphic basements.Primarily there are low-temperature and high-pressure blue schist,high-temperature and high-pressure granulite and ultrahigh-pressure (UHP)eclogite facies metamorphic rocks in the Phanerozoic orogenic belt.The discovery of eclogite in the Sulu orogen and a large quantity of coesite in its country rocks show that there was a deep subduction of voluminous continental materials during the collision process between the Yangtze block and the North China Craton in the Early Mesozoic phase.From the studies of,for instance,organic matter vitrinite reflectance,illite crystallinity,illite (muscovite)polytype and illite (muscovite)b dimension,the Late Paleozoic strata in the eastern region of Inner Mongolia and the north-central region of NE China have only experienced diagenesis to an extremely low-grade metamorphism.The discovery of impact-metamorphosed rocks in Xiuyan area of Liaoning province has enriched the type and category of metamorphic rocks in China.The phase equilibrium method has been widely used in the study of metamorphism of middle and high-grade metamorphic rocks.On the basis of existing geologic surveys and monographic study results, different scholars have respectively compiled 1:1500000 Metamorphic Geological Map and Specifications of Qinghai Tibet Pla展开更多
Background The transcription factor, repressor of GATA-3 （ROG）, can simultaneously suppress the expression of T helper cells （Thl and Th2） cytokines. Since the suppression of Th2 cytokines by GATA-3 is well unders...Background The transcription factor, repressor of GATA-3 （ROG）, can simultaneously suppress the expression of T helper cells （Thl and Th2） cytokines. Since the suppression of Th2 cytokines by GATA-3 is well understood, it is postulated that there are other molecular targets of ROG that can suppress the expression of the Thl cytokines. We hypothesized that ROG might suppress the stimulators of T lymphocyte cytokines such as CD3, CD28, and inducible costimulator （ICOS）, or indirectly enhance the expression of cytokine suppressors such as T lymphocyte-associated antigen-4 （CTLA-4） and CD45. The objective of this study was to clarify the molecular targets of ROG involved in suppressing Thl or Th2 cytokines. Methods Real-time quantitative PCR （RT-PCR） and Western blotting were performed to evaluate the mRNA and protein levels of CD3, CD28, ICOS, CTLA-4, and CD45 in Thl and Th2 cells during various levels of ROG expression. Enzyme-linked immunosorbent assay （ELISA） was used to measure the levels of interferon-y （IFN-y） and intedeukin （IL）-4 in culture media of Thl and Th2 cells. Results The results showed that the mRNA and protein levels of ROG were relatively low in Thl and Th2 cells （P 〈0.01）. After ROG-pcDNA3.1 transfection, the mRNA and protein level of ROG was significantly elevated, while the expression of ICOS, IFN-y, and IL-4 was markedly down-regulated （P 〈0.01）. Conversely, transfection of ROG-siRNA led to inhibition of ROG expression and up-regulation of ICOS, IFN-y and IL-4 （P 〈0.01）. However, the expression levels of CD3, CD28, CTLA-4 and CD45 did not change in either ROG-pcDNA3.1 or ROG-siRNA-transfected Thl and Th2 cells （P 〉0.05）. Conclusion It is concluded that ROG can inhibit the expression of Thl and Th2 cytokines by down-regulating the expression of ICOS, which might be a potential molecular target for asthma treatment.展开更多
基金financially supported by the National Natural Science Foundation of China(No.40906098)the Synthetic Investigation on the Environment in Polar Region (No.CHINARE 2013-02-02)the National Key Scientific Instrument and Equipment Development Project(No. 2012YQ09022907)
文摘A new ion chromatography coupled with tandem mass spectrometry （IC-ESI-MS/MS） method, with automated sampling and on-line preconcentration, has been developed for the determination of perchlorate in Antarctic snow and ice at low part-per-trillion （ng]L） levels. To the best of our lmowledge, this is the first time that an analytical method is used for the determination of perchlorate in Antarctic snow and ice. The IC-ESI-MS/MS instrumentation consisted of an ICS2000 ion chromatography （IC） system coupled to an API3200 electrospray tandem mass spectrometer （ESI-MS/MS）. On-line preconcentration was realized through a six-port injector valve, a TAC-ULP1 concentrator column and an AS auto-sampler. Multiple reaction monitoring （MRM） mode was used to quantify the perch/orate anion. The transition of 35Cl]604- （m/z 98.9） into 3SC11603 （m[z 82.9） was monitored for quantifying the main analyte, and the transition of 37C11604 （m/z 100.9） into 37C1~603- （m]z 84.9） was monitored for examining a proper isotopic abundance ratio of 3sCl to 37C1, which was used as a confirmation tool. The limit of detection （LOD） and limit of quantitation （LOQ） for the method was 0.2 ng/ L and 0.5 ng/L, respectively. And this new method exhibited acceptable accuracy and precision for samples at ng/L levels. All the tested snow and ice samples were found to contain measurable amount of oerchlorate, ran^in~ from 10 nell to 340 nell..
文摘Background： The efficacy and safety of telmisartan combined with clopidogrel, leflunomide, or both drugs for immunoglobulin A nephropathy （IgAN） are unclear. This study was designed to evaluate the efficacy and safety of telmisartan combined with clopidogrel, leflunomide, or both drugs for IgAN. Methods： It is a multicenter, prospective, double-dummy randomized controlled trial. Primary IgAN patients were recruited in 13 renal units across Beijing, China, from July 2010 to June 2012. After a 4-week telmisartan （80 mg/d） wash-in, 400 patients continuing on 80 mg/d telmisartan were randomly assigned to additionally receive placebo （Group A）, 50 mg/d clopidogrel （Group B）, 20 mg/d leflunomide （Group C）, or 50 mg/d clopidogrel and 20 mg/d leflunomide （Group D）. The 24-week intervention was completed by 360 patients. The primary endpoint was change in 24-h proteinuria at 24 weeks. A linear mixed-effect model was used to analyze the changes at 4, 12, and 24 weeks. Generalized estimating equations were used to evaluate changes in hematuria grade. This trial was registered at the Chinese Clinical Trial Registry. Results： The effects oftelmisartan combined with leflunomide on changes in proteinuria （0.36 [95% confidence interval （CI） 0.18 0.55] g/d, P 〈 0.001）, in serum uric acid （76.96 [95% CI 57.44-96.49] μmol/L, P 〈 0.001）, in serum creatinine （9.49 [95% CI 6.54-12.44]μmol/L, P 〈 0.001）, and in estimated glomerular filtration rate （-6.72 [95% CI-9.46 to -3.98] ml.min -1. 1.73 m -2, p 〈 0.001） were statistically significant, whereas they were not statistically significant on changes in systolic and diastolic blood pressure and weight （P 〉 0.05）.Telmisartan combined with clopidogrel had no statistical effect on any outcome, and there was no interaction between the interventions. No obvious adverse reactions were observed. Conclusions： Telmisartan combined with leflunomide, not clopidogrel, is safe and effective for decreasing proteinuria in certain IgAN
基金This work was supported by the National Natural Science Foundation of China （No.61101218 and No.81102673）,the New Star Program of Beijing Science and Technology Commission （No.2011111） and the National Basic Research Program of China （No.2011 CB944004）.
文摘Background Cisplatin （DDP） is one of most effective and most commonly used therapeutic agent in treating tumors,it can accumulate in the kidney and lead to acute renal failure.MicroRNA-181a can induce cell apoptosis by suppressing the expression of Bcl-2 family.In the present study,we investigated the role of microRNA-181a in the apoptosis of tubular epithelial cell induced by DDP.Methods HK-2 cells were cultured,transfected with microRNA-181a inhibitor for 48 hours,and stimulated with 50 μmol/L cisplatin for 24 hours.MicroRNA-181a expression was analyzed by real time PCR,and cell apoptosis was detected by flow cytometry.Moreover,Bcl-2 and Bcl-2-associated X protein （Bax） expression were measured by Western blotting.Results MicroRNA-181a expression significantly down-regulated in cells transfected with microRNA-181a inhibitor,compared with that in untransfectd cells （21.19±2.01 vs.38.87±1.97,P 〈0.05）.Cell apoptosis induced by DDP significantly decreased in cells transfected with MicroRNA-181a inhibitor.Compared with DDP treated cells alone,Bcl-2 expression strikingly was up-regulated and Bax expression was down-regulated in cells transfected with microRNA-181a inhibitor.Conclusion One pathway of DDP induces apoptosis of tubular epithelial cell by suppressing Bcl-2 expression is achieved by regulating the target gene of MicroRNA-181a.
文摘7075 aluminum alloy was used to obtain elbow parts by complex thixoextrusion and the microstructure evolution and mechanical properties during this process were studied by SEM,TEM and other analytical methods.The results show that different parts in 7075 aluminum alloy show quite different microstructures.The microstructure of the thin walls formed by back-extrusion is stratified,and the bottom of the parts formed by angular extrusion is obviously deformed.Shear forces contribute to the crushing of grains and the coarse second phase.The main strengthening phases in the material areηphase and E phase,whose amounts greatly decrease during heating to semi-solid and thixoextrusion.After heat treatment,they can precipitate evenly to improve the mechanical properties of the material.The average tensile strength of whole part after thixoextrusion and heat treatment is 485.49 MPa,while the average elongation is 5.49%.
文摘The microstructure, composition and shape of precipitated phase under as-cast and finished product state of 5Cr21Mn9Ni4N steel with different rare earth （RE） amount were studied. Mechanical properties of 5Cr21Mn9Ni4N steels withont RE addition and with RE added by 0. 2% in mass percent were tested respectively. The results indicate that the solid solution amount of RE is about 10^-6 -10^-5 order of magnitude in 5Cr21Mn9Ni4N steel. Dendrite of as-cast condition is refined obviously and dimension of interstitial phase is shortened when RE is added by 0.10%-0.20%. But the microstructure will be coarser if surplus RE is added. Precipitated phase under finished product state distributes evenly in nearly same size with RE added by 0. 2% which leads to a largely improved high temperature mechanical property.
基金supported by grants from the Youth Foundation of Luzhou Medical College of Luzhou City of Sichuan Province of China,No.[2010]108the Talent Fund of the Affiliated Hospital of Luzhou Medical College of Luzhou City of Sichuan Province of China,No.[2011]43
文摘Glaucoma, a type of optic neuropathy, is characterized by the loss of retinal ganglion cells. It remains controversial whether c-Jun N-terminal kinase （JNK） participates in the apoptosis of retinal ganglion cells in glaucoma. This study sought to explore a possible mechanism of action of JNK signaling pathway in glaucoma-induced retinal optic nerve damage. We established a mouse model of chronic ocular hypertension by reducing the aqueous humor followed by pho- tocoagulation using the laser ignition method. Results showed significant pathological changes in the ocular tissues after the injury. Apoptosis of retinal ganglion cells increased with increased intraocular pressure, as did JNK3 mRNA expression in the retina. These data indicated that the increased expression of JNK3 mRNA was strongly associated with the increase in intraocular pressure in the retina, and correlated positively with the apoptosis of retinal ganglion cells.
基金financially supported by Fuzhou science and technology project (Nos.2021-ZD-213 and 2020-Z-6)Fujian Provincial Department of Science and Technology(Nos.2021T3036,2020T3004,2020T3030 and 2020H0040)+2 种基金STS Science And Technology Project of the Chinese Academy of Sciences(No.KFJ-STS-QYZD-2021-09-001)Quanzhou Science and Technology Project (No.2020G17)the Joint Fund of the Yulin University and the Dalian National Laboratory for Clean Energy (No.2021009)。
文摘Metallic zinc is an excellent anode material for Zn-ion batteries,but the growth of Zn dendrite severely hinders its practical application.Herein,an efficient and economical cationic additive,poly dimethyl diallyl ammonium(PDDA) was reported,used in aqueous Zn-ion batteries electrolyte for stabilizing Zn anode.The growth of zinc dendrites can be significantly restrained by benefiting from the pronounced electrostatic shielding effect from PDDA on the Zn metal surface.Moreover,the PDDA is preferentially absorbed on Zn(002) plane,thus preventing unwanted side reactions on Zn anode.Owing to the introduction of a certain amount of PDDA additive into the common ZnSO_(4)-based electrolyte,the cycle life of assembled Zn‖Zn cells(1 mA·cm^(-2) and 1 mAh·cm^(-2)) is prolonged to more than 1100 h.In response to the perforation issue of Zn electrodes caused by PDDA additives,the problem can be solved by combining foamy copper with zinc foil.For real application,Zn-ion hybrid supercapacitors and MnO_(2)‖Zn cells were assembled,which exhibited excellent cycling stability with PDDA additives.This work provides a new solution and perspective to cope with the dendrite growth problem of Zn anode.
基金the Natural Science Foundation of the Fujian Province,No.2021J01876.
文摘BACKGROUND Gelastic seizure(GS)is a rare type of epilepsy that most commonly appears in patients with hypothalamic hamartoma.It is rarely associated with other types of brain damage.This particular type of epilepsy is relatively rare and has few links to other brain lesions.Temporal lobe malacia is mostly caused by cerebral infarction or cerebral hemorrhage,which can lead to seizures.We report a case of GS in a woman with temporal lobe malacia which was reported for the first time in the literature.CASE SUMMARY A 73-year-old female,diagnosed case of GS,presented with repetitive stereotyped laughter a month prior to presentation,happening multiple times daily and with each time lasting for 5-15s.Electroencephalogram displayed a focal seizure seen in the right temporal region.Magnetic resonance imaging head with contrast showed a right temporal lobe malacia.The patient was started on levetiracetam daily.The patient indicated that they had fully recovered and were not experiencing any recurrent or stereotyped laughter during their daily routines.These results remained consistent even after a one-year follow-up period.CONCLUSION GS can be caused by temporal lobe malacia,which is an uncommon but potentially grave condition.The outcome of this present case exhibited the importance of the temporal lobe in the genesis of GS.
基金supported by NSFC(Grant Nos.92061101,22271104,21871141,22225109,and 21901123)the Excellent Youth Foundation of Jiangsu Scientific Committee(BK20211593)+2 种基金the project funded by the China Postdoctoral Science Foundation(2018M630572)the Priority Academic Program Development of Jiangsu Higher Education Institutions,and the Foundation of Jiangsu Collaborative Innovation Center of Biomedical Functional Materials,the National Key Research and Development Project of China(Grant No.2021YFC2100100)the Natural Science Foundation of Jiangsu Province(Grant No.BK20190694)。
文摘The semi-hydrogenation of alkyne to form Z-olefins with high conversion and high selectivity is still a huge challenge in the chemical industry.Moreover,flammable and explosive hydrogen as the common hydrogen source of this reaction increases the cost and danger of industrial production.Herein,we connect the photocatalytic hydrogen evolution reaction and the semihydrogenation reaction of alkynes in series and successfully realize the high selective production of Z-alkenes using low-cost,safe,and green water as the proton source.Before the cascade reaction,a series of isomorphic metal–organic cage catalysts(Co_(x)Zn_(8−x)L_(6),x=0,3,4,5,and 8)are designed and synthesized to improve the yield of the photocatalytic hydrogen production.Among them,Co_(5)Zn_(3)L_(6) shows the highest photocatalytic activity,with a H_(2) generation rate of 8.81 mmol g^(−1) h^(−1).Then,Co_(5)Zn_(3)L_(6) is further applied in the above tandem reaction to efficiently reduce alkynes to Z-alkenes under ambient conditions,which can reach high conversion of>98%and high selectivity of>99%,and maintain original catalytic activity after multiple cycles.This“one-pot”tandem reaction can achieve a highly selective and safe stepwise conversion from water into hydrogen into Z-olefins under mild reaction conditions.
基金Supported by the Natural Science Foundation of Shanghai(23JC1400200)the National Natural Science Foundation of China(12225502,12075061,12147101)theNational KeyResearchandDevelopment ProgramofChina(2022YFA1604900)。
文摘The chiral magnetic wave(CMW)is a collective mode in quark-gluon plasma originated from the chiral magnetic effect(CME)and chiral separation effect.Its detection in heavy-ion collisions is challenging owing to significant background contamination.In[Y.S.Zhao et al.,Phys.Rev.C 106,L051901(2022)],we constructed a neural network that accurately identifies the CME-related signal from the final-state pion spectra.In this study,we have generalized this neural network to the case of CMW search.We show that,after an updated training,the neural network effectively recognizes the CMW-related signal.Additionally,we have assessed the performance of the neural network in comparison with other known methods for CMW search.
基金supported by Chinese National Natural Science Funding [grant number 82172710,2021]State Key Program for Chronic non-communicable Disease Prevention and Control of the Ministry of Science and Technology,China [grant number 2017YFC1309202,2017]+3 种基金Medical Innovation Research Project of Shanghai Science and Technology Commission (grant number 20Y11914400,2020)Outstanding Discipline Leader Project in Public Health in Shanghai (grant number GWVI-11.2-XD22,2023)Innovation Clinical Research Project of Shanghai Changzheng Hospital [grant number 2020YLCYJ-Z03,2020grant number 2023YJBF-FH05,2023].
文摘In the era of precision medicine,it has been increasingly recognized that individuals with a certain disease are complex and different from each other.Due to the underestimation of the significant heterogeneity across participants in traditional"one-sizefits-all"trials,patient-centered trials that could provide optimal therapy customization to individuals with specific biomarkers were developed including the basket,umbrella,and platform trial designs under the master protocol framework.In recent years,the successive FDA approval of indications based on biomarker-guided master protocol designs has demonstrated that these new clinical trials are ushering in tremendous opportunities.Despite the rapid increase in the number of basket,umbrella,and platform trials,the current clinical and research understanding of these new trial designs,as compared with traditional trial designs,remains limited.The majority of the research focuses on methodologies,and there is a lack of in-depth insight concerning the underlying biological logic of these new clinical trial designs.Therefore,we provide this comprehensive review of the discovery and development of basket,umbrella,and platform trials and their underlying logic from the perspective of precision medicine.Meanwhile,we discuss future directions on the potential development of these new clinical design in view of the"Precision Pro","Dynamic Precision",and"Intelligent Precision".This review would assist trial-related researchers to enhance the innovation and feasibility of clinical trial designs by expounding the underlying logic,which be essential to accelerate the progression of precision medicine.
基金Item Sponsored by Zhejiang Province Science Foundation of China[No.LQ12E05006]
文摘The effects of pulsed magnetic field on the solidified microstructure of Mg-Gd-Y-Zr alloy were investigated. Fine uniform equiaxed grains are acquired in the whole ingot by the pulsed magnetic field treatment,and the average grain size of the as-cast φ50 mm and φ100 mm ingots is refined to 37 μm and 47 μm with the pulsed magnetic field.The macrosegregation of solute elements of Mg-Gd-Y-Zr alloy is also reduced by the pulsed magnetic field treatment. Structure refinement is due to the electromagnetic undercooling zone in front of the solid/liquid interface by the magnetic pressure,and reduction of temperature gradient by the vibration of melt resulted from the pulsed magnetic field,which increases the nucleation rate and prohibits dendrite growth.In addition,primary α-Mg dendrites break into fine crystals, resulting in a refined solidification structure of the magnesium alloys.The Joule heat effect induced in the melt also strengthens the grain refinement effect and the spheroidization of dendrite arms.
基金financially supported by the National Key Research and Development Program of China(No.2016YFB0301105)the National Natural Science Foundation of China(No.51804190)+4 种基金the Shandong Provincial Natural Science Foundation,China(No.ZR2021ME240)the Youth Science Funds of Shandong Academy of Sciences,China(No.2020QN0022)the Shandong Province Key Research and Development Plan,China(Nos.2019GHZ019 and 2019JZZY020329)the Jinan Science&Technology Bureau,China(No.2019GXRC030)the Innovation Pilot Project for Fusion of Science,Education and Industry(International Cooperation)from Qilu University of Technology(Shandong Academy of Sciences),China(No.2020KJC-GH03)。
文摘In order to impart electrical conductivity to the magnesium alloy micro-arc oxidation(MAO)coating,the electroless copper plating was performed.Effects of plating temperature and complexing agent concentration on the properties of the electroless copper plating layers were studied by measuring their microstructure,corrosion resistance and electrical conductivity.It was found that the optimized plating temperature was 60°C,and the most suitable value of the complexing agent concentration was 30 g/L.Under this condition,a complete and dense plating layer could be obtained.The formation mechanism of the plating layer on magnesium alloy MAO coating was analyzed.A three-stage model of the plating process was proposed.The square resistance of the plated specimen was finally reduced to 0.03Ω/□after the third stage.Through electroless copper plating,the MAO coated sample obtained excellent electrical conductivity without significantly reducing its corrosion resistance.
基金financially supported by the National Key Technology Pillar Program(No.2013BAC11B05)Key Research and Innovation Program of Jiangsu Province(No.BE2015055)the Science&Technology Pillar Program in Sichuan Province(No.2016GZ0228)
文摘Mg-A1 mixed oxides with different Mg/A1 molar ratio were prepared by thermal decomposition of hydrotalcite- like precursors at 500 ℃ for 5.0 h and used as catalysts for the transesterification of diphenyl carbonate with 1,4-butanediol to synthesize high-molecular-weight poly（butylene carbonate） （PBC）. The structure-activity correlations of these catalysts in this transesterification process were discussed by means of various characterization techniques. It was found that the chain growth for the formation of PBC can only be obtained through connecting -OH and -OC（C）OC6H5 end-group upon removing the generated phenol, and the sample with Mg/A1 molar ratio of 4.0 exhibited the best catalytic performance, giving PBC with Mw of 1.64 × 105 g/mol at 210℃ for 3.0 h. This excellent activity depended mainly on the specific surface area and basicity rather than pore structure or crystallite size of MgO.
基金supported by the Natural Science Foundation of Shandong Province, China (ZR2021MC045)the Key Research & Development Plan (Major Scientific and Technological Innovation Project) of Shandong Province, China (2021LZGC024)the earmarked fund for China Agriculture Research System(CARS-27)。
文摘Cold stress is an important factor that limits apple production. In this study, we examined the tissue-cultured plantlets of apple rootstocks ‘M9T337' and ‘60-160', which are resistant and sensitive to cold stress, respectively. The enriched pathways of differentially expressed genes(DEGs) and physiological changes in ‘M9T337' and ‘60-160' plantlets were clearly different after cold stress(1°C) treatment for 48 h, suggesting that they have differential responses to cold stress. The differential expression of WRKY transcription factors in the two plantlets showed that MdWRKY40is and MdWRKY48 are potential regulators of cold tolerance. When we overexpressed MdWRKY40is and MdWRKY48in apple calli, the overexpression of MdWRKY48 had no significant effect on the callus, while MdWRKY40is overexpression promoted anthocyanin accumulation, increased callus cold tolerance, and promoted the expression of anthocyanin structural gene MdDFR and cold-signaling core gene MdCBF2. Yeast one-hybrid screening and electrophoretic mobility shift assays showed that MdWRKY40is could only bind to the MdDFR promoter. Yeast twohybrid screening and bimolecular fluorescence complementation showed that MdWRKY40is interacts with the CBF2inhibitor MdMYB15L through the leucine zipper(LZ). When the LZ of MdWRMY40is was knocked out, MdWRKY40is overexpression in the callus did not affect MdCBF2 expression or callus cold tolerance, indicating that MdWRKY40is acts in the cold signaling pathway by interacting with MdMYB15L. In summary, MdWRKY40is can directly bind to the MdDFR promoter in order to promote anthocyanin accumulation, and it can also interact with MdMYB15L to interfere with its inhibitory effect on MdCBF2, indirectly promoting MdCBF2 expression, and thereby improving cold tolerance.These results provide a new perspective for the cold-resistance mechanism of apple rootstocks and a molecular basis for the screening of cold-resistant rootstocks.
基金supported by Hunan Provincial Natural Science Foundation of China(11JJ3009)supported by the Scientific Research Foundation of Hunan Provincial Education Department(11C1187)the Construct Program of the Key Discipline in Hunan Province
文摘In this paper, we study the multiplicity of positive solutions to the following m-point boundary value problem of nonlinear fractional differential equations： Dqu（t） ＋ f（t, u（t）） = 0, 0 t 1, u（0） = 0, u（1） =sum （μiDpu（t）｜t = ξi ） from i =1 to ∞ m-2, where q ∈R , 1 q ≤2 , 0 ξ1 ξ2 ··· ξm-2 ≤ 1/2 , μi ∈[0 , ＋∞） and p = q-1/2 , Γ（q） sum （μiξi（q-1）/2 Γ（（ q＋1）/2） from i =1 to ∞ m-2,Dq is the standard Riemann-Liouville differentiation, and f ∈C （[0 , 1]×[0 , ＋∞） , [0 , ＋∞））. By using the Leggett-Williams fixed point theorem on a convex cone, some multiplicity results of positive solutions are obtained.
基金the National Natural Science Foundation of China (41203025)the Work Project of the China Geological Survey (1212010811048)the Fundamental Research Fund of the Institute of Geology of the Chinese Academy of Geological Sciences (J1615).
文摘The development of metamorphic petrology to metamorphic geology in China has a long history.Ancient basement metamorphic rocks are distributed primarily in the North China Craton,the Yangtze Block and Tarim Craton.They are mainly made up of plutonic gneiss and metamorphosed supercrust rock,transformed to granulite facies through Archean Paleoproterozoic.Many of the Paleoproterozoic metamorphic rocks have undergone high-pressure granulite facies metamorphism with a clockwise metamorphic evolution path.The ultrahigh temperature (UHT)granulites from the Late Paleoproterozoic are found in North China Craton.Many high-precision chronological data have allowed preliminary construction of the formation and evolutionary framework of different metamorphic basements.Primarily there are low-temperature and high-pressure blue schist,high-temperature and high-pressure granulite and ultrahigh-pressure (UHP)eclogite facies metamorphic rocks in the Phanerozoic orogenic belt.The discovery of eclogite in the Sulu orogen and a large quantity of coesite in its country rocks show that there was a deep subduction of voluminous continental materials during the collision process between the Yangtze block and the North China Craton in the Early Mesozoic phase.From the studies of,for instance,organic matter vitrinite reflectance,illite crystallinity,illite (muscovite)polytype and illite (muscovite)b dimension,the Late Paleozoic strata in the eastern region of Inner Mongolia and the north-central region of NE China have only experienced diagenesis to an extremely low-grade metamorphism.The discovery of impact-metamorphosed rocks in Xiuyan area of Liaoning province has enriched the type and category of metamorphic rocks in China.The phase equilibrium method has been widely used in the study of metamorphism of middle and high-grade metamorphic rocks.On the basis of existing geologic surveys and monographic study results, different scholars have respectively compiled 1:1500000 Metamorphic Geological Map and Specifications of Qinghai Tibet Pla
基金This study was supported by grants from the National Natural Science Foundation of China （No. 30900660, No. 30871134 and No. 81172227） and Shanghai Committee of Science and Technology of China （No. 08JC1407600）. Conflict of interests： None.
文摘Background The transcription factor, repressor of GATA-3 （ROG）, can simultaneously suppress the expression of T helper cells （Thl and Th2） cytokines. Since the suppression of Th2 cytokines by GATA-3 is well understood, it is postulated that there are other molecular targets of ROG that can suppress the expression of the Thl cytokines. We hypothesized that ROG might suppress the stimulators of T lymphocyte cytokines such as CD3, CD28, and inducible costimulator （ICOS）, or indirectly enhance the expression of cytokine suppressors such as T lymphocyte-associated antigen-4 （CTLA-4） and CD45. The objective of this study was to clarify the molecular targets of ROG involved in suppressing Thl or Th2 cytokines. Methods Real-time quantitative PCR （RT-PCR） and Western blotting were performed to evaluate the mRNA and protein levels of CD3, CD28, ICOS, CTLA-4, and CD45 in Thl and Th2 cells during various levels of ROG expression. Enzyme-linked immunosorbent assay （ELISA） was used to measure the levels of interferon-y （IFN-y） and intedeukin （IL）-4 in culture media of Thl and Th2 cells. Results The results showed that the mRNA and protein levels of ROG were relatively low in Thl and Th2 cells （P 〈0.01）. After ROG-pcDNA3.1 transfection, the mRNA and protein level of ROG was significantly elevated, while the expression of ICOS, IFN-y, and IL-4 was markedly down-regulated （P 〈0.01）. Conversely, transfection of ROG-siRNA led to inhibition of ROG expression and up-regulation of ICOS, IFN-y and IL-4 （P 〈0.01）. However, the expression levels of CD3, CD28, CTLA-4 and CD45 did not change in either ROG-pcDNA3.1 or ROG-siRNA-transfected Thl and Th2 cells （P 〉0.05）. Conclusion It is concluded that ROG can inhibit the expression of Thl and Th2 cytokines by down-regulating the expression of ICOS, which might be a potential molecular target for asthma treatment.
