Objective: Rheumatoid arthritis(RA) is a chronic inflammatory and destructive arthritis, characterized by inflammatory infiltration and bone destruction. Huangqi Guizhi Wuwu Decoction(HGWD) is traditional Chinese medi...Objective: Rheumatoid arthritis(RA) is a chronic inflammatory and destructive arthritis, characterized by inflammatory infiltration and bone destruction. Huangqi Guizhi Wuwu Decoction(HGWD) is traditional Chinese medicine, which has been applied in the treatment of RA in clinical. The aim of this study was to investigate the therapeutic effect of HGWD on collagen-induced arthritis(CIA) mouse model.Methods: DBA/1J female mice were used to establish the collagen-induced arthritis(CIA) model. HGWD was administered intragastrically once a day for four weeks starting on the 22nd day after the first immunization. The body weight, hind paw thickness and clinical score were measured every five days. Gait analysis, histopathological staining, enzyme-linked immunosorbent assay(ELISA), ultrasound imaging and micro-computed tomography imaging were performed to determine the effects of HGWD treatment on inflammation and bone structure in this model. Moreover, Real-time PCR and Western blot analysis were used to detect inflammatory factors m RNA and protein levels after HGWD intervention in RAW264.7 cells.Results: HGWD attenuated symptoms of arthritis, suppressed inflammatory synovium area and the serum levels of inflammatory factors, inhibited joint space enlargement in the knee and ankle joints,reduced numbers of osteoclasts, protected bone destruction, as well as improved motor function.HGWD decreased the expression of m RNA for inflammatory factors and the protein expression levels of p-NF-ΚB and IL-17.Conclusion: These results suggested that HGWD suppresses inflammation, attenuates bone erosion and maintains motor function in collagen-induced arthritis mice.展开更多
Although,great achievements have been made in the synthesis of heterocycles using radical addition/cyclization strategy,developing versatile alkyl radical precursors,especially the non-stabilized long chain alkyl radi...Although,great achievements have been made in the synthesis of heterocycles using radical addition/cyclization strategy,developing versatile alkyl radical precursors,especially the non-stabilized long chain alkyl radicals for this strategy still remains a huge challenge.Herein,we report an efficient annulation cascade reaction between cyclosulfonium salts and alkenes for the synthesis of sulfur-containing N-heterocycles by visible light/copper catalysis under mild conditions.The C—S bond cleavage/radical cascade reaction delivers a variety of corresponding N-heterocycles containing aryl alkyl thioether motifs with good functional group tolerance.Significantly,the current system could be used for the late-stage functionalization of complex bioactive molecules.展开更多
A mathematical formulation is presented for the dynamic stress intensity factor (mode I) of a finite permeable crack subjected to a time-harmonic propagating longitudinal wave in an infinite poroelastic solid. In part...A mathematical formulation is presented for the dynamic stress intensity factor (mode I) of a finite permeable crack subjected to a time-harmonic propagating longitudinal wave in an infinite poroelastic solid. In particular, the effect of the wave-induced fluid flow due to the presence of a liquid-saturated crack on the dynamic stress intensity factor is analyzed. Fourier sine and cosine integral transforms in conjunction with Helmholtz potential theory are used to formulate the mixed boundary-value problem as dual integral equations in the frequency domain. The dual integral equations are reduced to a Fredholm integral equation of the second kind. It is found that the stress intensity factor monotonically decreases with increasing frequency, decreasing the fastest when the crack width and the slow wave wavelength are of the same order. The characteristic frequency at which the stress intensity factor decays the fastest shifts to higher frequency values when the crack width decreases.展开更多
Crack problems are often reduced to dual integral equations,which can be solved by expanding the displacement integral equation as a series in the form of Chebyshev-like or Jacobi polynomials.Schmidt’s multiplying-fa...Crack problems are often reduced to dual integral equations,which can be solved by expanding the displacement integral equation as a series in the form of Chebyshev-like or Jacobi polynomials.Schmidt’s multiplying-factor integration method has been one of the most favorable techniques for determining the expansion coefficients by constructing a well-posed system of linear algebraic equations.However,Schmidt’s method is less efficient for numerical computation because the matrix elements of the linear equations are evaluated from dual integrals.In this study,we propose a modified method to construct linear equations to efficiently determine the expansion coefficients.The modified technique is developed upon the application of certain multiplying factors to the traction integral equation and then integrating the resulting equation over“source”regions.Such manipulations simplify the matrix elements as single integrals.By carrying out numerical examples,we demonstrate that the technique is not only accurate but also very efficient.In particular,the method only needs approximately 1/5 of the computation time of Schmidt’s method.Therefore,this method can be used to replace Schmidt’s method and is expected to be very useful in solving crack problems.展开更多
目的观察经筋推拿手法治疗早中期膝骨关节炎的临床疗效.方法将62例早中期膝骨关节炎患者随机分为治疗组和对照组各31例,对照组给予早中期膝骨关节炎常规治疗,治疗组在其基础上给予经筋推拿手法治疗,日1次,两组均以2周为1疗程,共治疗两...目的观察经筋推拿手法治疗早中期膝骨关节炎的临床疗效.方法将62例早中期膝骨关节炎患者随机分为治疗组和对照组各31例,对照组给予早中期膝骨关节炎常规治疗,治疗组在其基础上给予经筋推拿手法治疗,日1次,两组均以2周为1疗程,共治疗两个疗程.治疗前、治疗两个疗程后以膝关节疼痛视觉模拟量表(Visual Analog Scale,VAS)评分及西安大略和麦克马斯特国际骨关节炎调查量表(Western Ontario and Mc-Master Universities Osteoarthritis Index,WOMAC)作为疗效评估指标,参照《中药新药临床研究指导原则》中对膝骨关节炎的疗效评价标准比较2组患者的总体疗效.结果膝关节疼痛VAS评分、WOMAC评分比较,两组差异有统计学意义(P<0.05).总体疗效方面,治疗两个疗程后,治疗组有效25例,显效4例,无效2例;对照组有效12例,显效13例,无效6例;治疗组的总体疗效优于对照组(Z=-3.228,P=0.001).结论经筋推拿手法在早中期膝骨关节炎疼痛、僵滞等症状缓解及功能活动改善方面疗效可靠.展开更多
基金sponsored by National Natural Science Foundation(No.81822050,81920108032,81904227)Shanghai“Science and Technology Innovation Action Plan”Medical Innovation Research Project(No.21Y11921400)+4 种基金the Program for Innovative Research Team of Ministry of Science and Technology of China(No.2015RA4002)“Innovation Team”Development Projects(No.IRT1270)Innovative Team Project of Scientific Research Project of Traditional Chinese Medicine of Shanghai Municipal Health Commission(No.2022CX001)Shanghai TCM Medical Center of Chronic Disease(No.2022ZZ01009)Jing'an District Health Research Project of Shanghai(No.2022MS03).
文摘Objective: Rheumatoid arthritis(RA) is a chronic inflammatory and destructive arthritis, characterized by inflammatory infiltration and bone destruction. Huangqi Guizhi Wuwu Decoction(HGWD) is traditional Chinese medicine, which has been applied in the treatment of RA in clinical. The aim of this study was to investigate the therapeutic effect of HGWD on collagen-induced arthritis(CIA) mouse model.Methods: DBA/1J female mice were used to establish the collagen-induced arthritis(CIA) model. HGWD was administered intragastrically once a day for four weeks starting on the 22nd day after the first immunization. The body weight, hind paw thickness and clinical score were measured every five days. Gait analysis, histopathological staining, enzyme-linked immunosorbent assay(ELISA), ultrasound imaging and micro-computed tomography imaging were performed to determine the effects of HGWD treatment on inflammation and bone structure in this model. Moreover, Real-time PCR and Western blot analysis were used to detect inflammatory factors m RNA and protein levels after HGWD intervention in RAW264.7 cells.Results: HGWD attenuated symptoms of arthritis, suppressed inflammatory synovium area and the serum levels of inflammatory factors, inhibited joint space enlargement in the knee and ankle joints,reduced numbers of osteoclasts, protected bone destruction, as well as improved motor function.HGWD decreased the expression of m RNA for inflammatory factors and the protein expression levels of p-NF-ΚB and IL-17.Conclusion: These results suggested that HGWD suppresses inflammation, attenuates bone erosion and maintains motor function in collagen-induced arthritis mice.
基金the National Natural Science Foundation of China(22271170)the Scientific Research Foundation of Qingdao University of Science and Technology.
文摘Although,great achievements have been made in the synthesis of heterocycles using radical addition/cyclization strategy,developing versatile alkyl radical precursors,especially the non-stabilized long chain alkyl radicals for this strategy still remains a huge challenge.Herein,we report an efficient annulation cascade reaction between cyclosulfonium salts and alkenes for the synthesis of sulfur-containing N-heterocycles by visible light/copper catalysis under mild conditions.The C—S bond cleavage/radical cascade reaction delivers a variety of corresponding N-heterocycles containing aryl alkyl thioether motifs with good functional group tolerance.Significantly,the current system could be used for the late-stage functionalization of complex bioactive molecules.
基金supported by the National Natural Science Foundation of China (Grant 11372091)China Scholarship Council (Grant 201406120086)
文摘A mathematical formulation is presented for the dynamic stress intensity factor (mode I) of a finite permeable crack subjected to a time-harmonic propagating longitudinal wave in an infinite poroelastic solid. In particular, the effect of the wave-induced fluid flow due to the presence of a liquid-saturated crack on the dynamic stress intensity factor is analyzed. Fourier sine and cosine integral transforms in conjunction with Helmholtz potential theory are used to formulate the mixed boundary-value problem as dual integral equations in the frequency domain. The dual integral equations are reduced to a Fredholm integral equation of the second kind. It is found that the stress intensity factor monotonically decreases with increasing frequency, decreasing the fastest when the crack width and the slow wave wavelength are of the same order. The characteristic frequency at which the stress intensity factor decays the fastest shifts to higher frequency values when the crack width decreases.
基金This work was supported by the National Natural Science Foundation of China(Grant Nos.11802074,42074057,11972132,and 11734017)China National Postdoctoral Program for Innovative Talents(Grant No.BX201700066)+1 种基金China Postdoctoral Science Foundation(Grant No.2018M630345)the Fundamental Research Funds for the Central Universities(Grant No.HIT.NSRIF.2020016)。
文摘Crack problems are often reduced to dual integral equations,which can be solved by expanding the displacement integral equation as a series in the form of Chebyshev-like or Jacobi polynomials.Schmidt’s multiplying-factor integration method has been one of the most favorable techniques for determining the expansion coefficients by constructing a well-posed system of linear algebraic equations.However,Schmidt’s method is less efficient for numerical computation because the matrix elements of the linear equations are evaluated from dual integrals.In this study,we propose a modified method to construct linear equations to efficiently determine the expansion coefficients.The modified technique is developed upon the application of certain multiplying factors to the traction integral equation and then integrating the resulting equation over“source”regions.Such manipulations simplify the matrix elements as single integrals.By carrying out numerical examples,we demonstrate that the technique is not only accurate but also very efficient.In particular,the method only needs approximately 1/5 of the computation time of Schmidt’s method.Therefore,this method can be used to replace Schmidt’s method and is expected to be very useful in solving crack problems.
文摘目的观察经筋推拿手法治疗早中期膝骨关节炎的临床疗效.方法将62例早中期膝骨关节炎患者随机分为治疗组和对照组各31例,对照组给予早中期膝骨关节炎常规治疗,治疗组在其基础上给予经筋推拿手法治疗,日1次,两组均以2周为1疗程,共治疗两个疗程.治疗前、治疗两个疗程后以膝关节疼痛视觉模拟量表(Visual Analog Scale,VAS)评分及西安大略和麦克马斯特国际骨关节炎调查量表(Western Ontario and Mc-Master Universities Osteoarthritis Index,WOMAC)作为疗效评估指标,参照《中药新药临床研究指导原则》中对膝骨关节炎的疗效评价标准比较2组患者的总体疗效.结果膝关节疼痛VAS评分、WOMAC评分比较,两组差异有统计学意义(P<0.05).总体疗效方面,治疗两个疗程后,治疗组有效25例,显效4例,无效2例;对照组有效12例,显效13例,无效6例;治疗组的总体疗效优于对照组(Z=-3.228,P=0.001).结论经筋推拿手法在早中期膝骨关节炎疼痛、僵滞等症状缓解及功能活动改善方面疗效可靠.
