A pot experiment and a sandy culture experiment grown with three vegetable crops of Chinese cabbage ( B. chinensis L., cv. Zao\|Shu 5), winter greens ( B.var.rosularis Tsen et Lee, cv. Shang\|Hai\|Qing) and cele...A pot experiment and a sandy culture experiment grown with three vegetable crops of Chinese cabbage ( B. chinensis L., cv. Zao\|Shu 5), winter greens ( B.var.rosularis Tsen et Lee, cv. Shang\|Hai\|Qing) and celery ( A.graveolens L. var. dulce DC., cv. Qing\|Qin) were conducted, respectively. The initial soil and four incubated soils with different extractable Cd (0.15, 0.89, 1.38, 1.84 and 2.30 mg Cd/kg soil) were used for the pot experiment. Five treatments were designed (0, 0.0625, 0.125, 0.250 and 0.500 mg Cd/L) in nutrient solution in the sandy culture experiment. Each treatment in pot and sandy culture experiments was trireplicated. The objectives of the study were to examine Cd accumulation in edible parts of selected vegetable crops, its correlation with Cd concentrations in vegetable garden soil or in nutrient solution, and evaluate the criteria of Cd pollution in vegetable garden soil and in nutrient solution based on the hygienic limit of Cd in vegetables. Cadmium concentrations in edible parts of the three selected vegetable crops were as follows: 0.01—0.15 mg/kg fresh weight for Chinese cabbage, 0.02—0.17 mg/kg fresh weight for winter greens, and 0.02—0.24 mg/kg fresh weight for celery in the pot experiment, and 0.1—0.4 mg/kg fresh weight for Chinese cabbage, 0.1—1.4 mg/kg fresh weight for winter greens, and 0.05—0.5 mg/kg fresh weight for celery in the pot experiment(except no\|Cd treatment). The order of the three test vegetable crops for cadmium accumulation in the edible parts was celery>winter greens>Chinese cabbage in both the pot experiment and the sandy culture experiment. Cadmium accumulation in edible parts or roots of the vegetable crops increased with increasing of cadmium concentration in the medium(soil or nutrient solution). And cadmium concentrations in edible parts of the test vegetable crops were significantly linearly related to the Cd levels in the growth media (soil and nutrient solution). Based on the regression equations established and the limit of cad展开更多
Dear Editor,Base editors(BEs)based on the CRISPR/Cas9 system,including cytosine base editors and adenine base editors,which can efficiently perform four transition mutations(C·G-to-T·A and A·T-to-G·...Dear Editor,Base editors(BEs)based on the CRISPR/Cas9 system,including cytosine base editors and adenine base editors,which can efficiently perform four transition mutations(C·G-to-T·A and A·T-to-G·C),have been well studied and widely used to produce base mutations in a variety of organisms,including in plants such as rice.展开更多
This paper explores the capability of the“surface-protected etching”process for the creation of rattle-type SiO_(2)@void@SiO_(2) colloidal structures featuring a mesoporous silica shell and a mesoporous movable sili...This paper explores the capability of the“surface-protected etching”process for the creation of rattle-type SiO_(2)@void@SiO_(2) colloidal structures featuring a mesoporous silica shell and a mesoporous movable silica core.The surface-protected etching process involves stabilization of the particle surface using a polymer ligand,and then selective etching of the interior to form hollow structures.In this paper,this strategy has been extended to the formation of rattle-like structures by etching SiO_(2)@SiO_(2) core shell particles which are synthesized by a two-step sol gel process.The key is to introduce a protecting polymer of polyvinylpyrrolidone(PVP)to the surface of both core and shell in order to tailor their relative stability against chemical etching.Upon reacting with NaOH,the outer layer silica becomes a hollow shell as only the surface layer is protected by PVP and the interior is removed,while the core remains its original size thanks to the protection of PVP on its surface.This process can be carried out at room temperature without the need of additional templates or complicated heterogeneous coating procedures.The etching process also results in the rattle-type colloids having mesoscale pores with two distinct average sizes.In our demonstration of a model drug delivery process,such mesoporous structures show an interesting two-step elution profile which is believed to be related to the unique porous rattle structures.展开更多
During last decades, sensor elements based on the fiber Bragg grating （FBG） have been widely studied and developed due to the advantages of immunity to electromagnetic interference, compact size, high precision, and...During last decades, sensor elements based on the fiber Bragg grating （FBG） have been widely studied and developed due to the advantages of immunity to electromagnetic interference, compact size, high precision, and so on. The FBG itself is sensitive to axial strain and temperature variation directly and can indirectly measure these complex physical parameters, such as pressure, displacement, and vibration, by using some specially designed elastic structures to convert them into the axial strain of the FBG. Whether the FBG is fixed on the measured object to measure the strain directly or fixed on an elastic structure body to measure other physical quantities, these types of FBGs could be collectively called as strain sensing FBGs. The packaging of the FBG has important influence on FBG characteristics that directly affect the measurement accuracy, such as strain transfer, temperature characteristic, and spectral shape. This paper summarizes the packaging methods and corresponding temperature compensation methods of the currently reported strain sensing FBGs, focusing especially on fully pasted FBG, pre-stretched FBG with double-end fixed, and metallic packaging. Furthermore, the advantages and drawbacks of different packaging methods have been analyzed, which can provide a reference for future researches.展开更多
Stripe rust and powdery mildew are both devastating diseases for durum and common wheat.Pyramiding of genes conferring resistance to one or more diseases in a single cultivar is an important breeding approach to provi...Stripe rust and powdery mildew are both devastating diseases for durum and common wheat.Pyramiding of genes conferring resistance to one or more diseases in a single cultivar is an important breeding approach to provide broader spectra of resistances in wheat improvement. A new powdery mildew resistance gene originating from wild emmer(Triticum turgidum var.dicoccoides) backcrossed into common wheat(T. aestivum) line WE35 was identified. It conferred an intermediate level of resistance to Blumeria graminis f. sp. tritici isolate E09 at the seedling stage and a high level of resistance at the adult plant stage. Genetic analysis showed that the powdery mildew resistance in WE35 was controlled by a dominant gene designated Pm64. Bulked segregant analysis(BSA) and molecular mapping indicated that Pm64 was located in chromosome bin 2 BL4-0.50–0.89. Polymorphic markers were developed from the corresponding genomic regions of Chinese Spring wheat and wild emmer accession Zavitan to delimit Pm64 to a 0.55 cM genetic interval between markers WGGBH1364 and WGGBH612, corresponding to a 15 Mb genomic region on Chinese Spring and Zavitan 2 BL, respectively. The genetic linkage map of Pm64 is critical for fine mapping and cloning. Pm64 was completely linked in repulsion with stripe rust resistance gene Yr5. Analysis of a larger segregating population might identify a recombinant line with both genes as a valuable resource in breeding for resistance to powdery mildew and stripe rust.展开更多
The development of CRISPR/Cas9-mediated base editing has made genomic modification more efficient. However, selection of genetically modified cells from millions of treated cells, especially plant cells, is still chal...The development of CRISPR/Cas9-mediated base editing has made genomic modification more efficient. However, selection of genetically modified cells from millions of treated cells, especially plant cells, is still challenging. In this study, an efficient surrogate reporter system based on a defective hygromycin resistance gene was established in rice to enrich base-edited cells. After step-by-step optimization, the Discriminated sgRNAs-based SurroGate system (DisSUGs) was established by artificially differentiating the editing abilities of a wild-type single guide RNA (sgRNA) targeting the surrogate reporter gene and an enhanced sgRNA targeting endogenous sites. The DisSUGs enhanced the efficiency of screening base-edited cells by 3- to 5-fold for a PmCDA1-based cytosine-to-tyrosine base editor (PCBE), and 2.5- to 6.5-fold for an adenine base editor (ABE) at endogenous targets. These targets showed editing efficiencies of <25% in the conventional systems. The DisSUGs greatly enhanced the frequency of homozygous substitutions and expanded the activity window slightly for both a PCBE and an ABE. Analyses of the total number of single-nucleotide variants from whole-genome sequencing revealed that, compared with the no-enrichment PCBE strategy, the DisSUGs did not alter the frequency of genome-wide sgRNA-independent off-target mutations, but slightly increased the frequency of target-dependent off-target mutations. Collectively, the DisSUGs developed in this study greatly enhances the efficiency of screening plant base-edited cells and will be a useful system in future applications.展开更多
Microstructure and mechanical behavior of high volume content SiCp/7xxxAl composites have not been explored yet. Therefore, in the present work, 45 vol.% SiCp/7075Al composite has been prepared by pres- sure infiltrat...Microstructure and mechanical behavior of high volume content SiCp/7xxxAl composites have not been explored yet. Therefore, in the present work, 45 vol.% SiCp/7075Al composite has been prepared by pres- sure infiltration method. High density dislocations were found around SiC/Al interface in SiCp/7075Al composite after water-quenching and aging treatment. Fine dispersed nano-η＇ phases were observed after the aging treatment. Adverse to other SiCp/Al composites prepared by the pressure infiltration method, an interface layer was observed between SiC particles and AI matrix. Furthermore, high-resolution trans- mission electron microscopy （HRTEM） observation indicated that this interface layer was coherent/semi- coherent with that of the SiC particles. 45 vol.% SiCp/7075Al composite demonstrated high tensile strength （630 MPa） and micro-ductility. Compared to aged SiCp/2024Al composite, the aged SiCp/7075Al com- posite showed an increase of about 200% in the tensile strain and 90% in the tensile strength, respectively. It is speculated that nano-η＇ phases in the Al matrix significantly contributed to the strengthening effect while the interface layer between SiC and AI matrix might be beneficial to the strength and plasticity of SiCp/7075Al composite.展开更多
Electron beam selective melting （EBSM） is a promising additive manufacturing （AM） technology. The EBSM process consists of three major procedures：（1） spreading a powder layer, （2） preheating to slightly sinte...Electron beam selective melting （EBSM） is a promising additive manufacturing （AM） technology. The EBSM process consists of three major procedures：（1） spreading a powder layer, （2） preheating to slightly sinter the powder, and （3） selectively melting the powder bed. The highly transient multi-physics phenomena involved in these procedures pose a significant challenge for in situ experimental observation and measurement. To advance the understanding of the physical mechanisms in each procedure, we leverage high- fidelity modeling and post-process experiments. The models resemble the actual fabrication procedures, including （1） a powder-spreading model using the discrete element method （DEM）, （2） a phase field （PF） model of powder sintering （solid-state sintering）, and （3） a powder-melting （liquid-state sintering） model using the finite volume method （FVM）. Comprehensive insights into all the major procedures are provided, which have rarely been reported. Preliminary simulation results （including powder particle packing within the powder bed, sintering neck formation between particles, and single-track defects） agree qualitatively with experiments, demonstrating the ability to understand the mechanisms and to guide the design and optimization of the experimental setup and manufacturing process.展开更多
A brief review of recent progress in researches, productions and applications of full distributed fiber Raman photon sensors at China Jiliang University （CJLU） is presented. In order to improve the measurement dista...A brief review of recent progress in researches, productions and applications of full distributed fiber Raman photon sensors at China Jiliang University （CJLU） is presented. In order to improve the measurement distance, the accuracy, the space resolution, the ability of multi-parameter measurements, and the intelligence of full distributed fiber sensor systems, a new generation fiber sensor technology based on the optical fiber nonlinear scattering fusion principle is proposed. A series of new generation full distributed fiber sensors are investigated and designed, which consist of new generation ultra-long distance full distributed fiber Raman and Rayleigh scattering photon sensors integrated with a fiber Raman amplifier, auto-correction full distributed fiber Raman photon temperature sensors based on Raman correlation dual sources, full distributed fiber Raman photon temperature sensors based on a pulse coding source, full distributed fiber Raman photon temperature sensors using a fiber Raman wavelength shifter, a new type of Brillouin optical time domain analyzers （BOTDAs） integrated with a fiber Raman amplifier for replacing a fiber Brillouin amplifier, full distributed fiber Raman and Brillouin photon sensors integrated with a fiber Raman amplifier, and full distributed fiber Brillouin photon sensors integrated with a fiber Brillouin frequency shifter. The Internet of things is believed as one of candidates of the next technological revolution, which has driven hundreds of millions of class markets. Sensor networks are important components of the Internet of things. The full distributed optical fiber sensor network （Rayleigh, Raman, and Brillouin scattering） is a 3S （smart materials, smart structure, and smart skill） system, which is easy to construct smart fiber sensor networks. The distributed optical fiber sensor can be embedded in the power grids, railways, bridges, tunnels, roads, constructions, water supply systems, dams, oil and gas pipelines and other facilities, and can be integrate展开更多
The Satellite Positioning and Orbit Determination System(SPODS)is a software package for GNSS positioning/orbit determination,developed by the Xi’an Research Institute of Surveying and Mapping.So far it has been able...The Satellite Positioning and Orbit Determination System(SPODS)is a software package for GNSS positioning/orbit determination,developed by the Xi’an Research Institute of Surveying and Mapping.So far it has been able to analyse GPS data and has the capability of high precision GPS positioning and orbit determination.The underlying theory and the performance evaluation are briefly addressed in this paper.The experiments are carried out with GPS data collected from about 127 IGS stations during 4~10 January 2009.The results show that the RMS 1D difference is 1.1 cm between SPODS orbits and final IGS combined orbits,and that the repeatability of daily solutions of station coordinates is 1.5 mm for horizontal components,and 4.5 mm for vertical component,and that the consistency of ERP solutions with IGS final products is 0.025 mas,0.093 mas and 0.013 ms/d respectively for pole coordinates and LOD.展开更多
Stromal cell-derived factor-1 and its receptor CXC chemokine receptor-4(CXCR4)have been implicated in breast cancer metastasis.A significant association between HER2 and CXCR4 expression has been observed in human bre...Stromal cell-derived factor-1 and its receptor CXC chemokine receptor-4(CXCR4)have been implicated in breast cancer metastasis.A significant association between HER2 and CXCR4 expression has been observed in human breast tumor tissues,and overexpression of CXCR4 is essential for HER2-mediated tumor metastasis.Moreover,CXCR4 expression is low in normal breast tissues and high in malignant tumors,suggesting that a blockade of CXCR4 may limit tumor metastasis.The present study investigated the action of a synthetic antagonist 21-mer peptide derived from viral macrophage inflammatory protein Ⅱ against CXCR4(NT21MP)in inhibiting metastasis in vitro and in vivo.The results showed that chemotaxis of SKBR3 cells toward SDF-1αwas reduced by NT21MP in a dose-dependent manner(P<0.05).NT21MP inhibited tumor growth at 500μg/kg and in combination with Herceptin,the anti-HER2 antibody.The in vivo metastatic assay showed that NT21MP significantly inhibited pulmonary metastasis,and the number of metastatic tumor nodes on the surface of the lung was greatly decreased.Compared with the saline-treated control group,PCNA expression was dose-dependently decreased by NT21MP,the percentage of apoptotic cells was increased,and CXCR4 mRNA and protein expression were downregulated.In conclusion,NT21MP inhibits cellular proliferation,promotes apoptosis by downregulating CXCR4 expression,and suppresses the progression of primary and metastatic tumors.CXCR4 may be a useful therapeutic target for breast cancer,and NT21MP may serve as a potential target drug for the treatment of breast cancer metastasis.展开更多
Stripe rust(yellow rust), caused by Puccinia striiformis f. sp. tritici(PST),is one of the most devastating fungal diseases in common wheat(Triticum aestivum L.) in China and worldwide. Resistance breeding is the most...Stripe rust(yellow rust), caused by Puccinia striiformis f. sp. tritici(PST),is one of the most devastating fungal diseases in common wheat(Triticum aestivum L.) in China and worldwide. Resistance breeding is the most effective strategy to control diseases in crop plants. Chinese wheat lines Mengmai 58 and Huaiyang 1 are highly resistant to PST race CYR34(V26) at the adult plant stage. To genetically map the underlying resistance genes we developed segregating populations by crossing Mengmai 58 and Huaiyang 1 with the susceptible cultivar Nongda 399. The stripe rust resistances in Mengmai 58 and Huaiyang 1 were both controlled by single dominant genes, provisionally designated YrMM58 and YrHY1, respectively. Bulked segregant RNA-Seq(BSR-Seq) analysis showed that YrMM58 and YrHY1 were located in the same distal ~16 Mb region on chromosome 2 AS.Comparative genomics analysis with the physical map of Aegilops tauschii proved useful for developing additional markers to saturate the genetic linkage map. YrMM58 and YrHY1 were mapped to the distal end of chromosome arm 2 AS, with the closest marker WGGB148 being 7.7 cM and 3.8 cM from the resistance gene, which was considered to be Yr17. These markers can be used in marker-assisted selection.展开更多
Leaf senescence is normally the last stage of plant development. Early senescence of functional leaves significantly reduces the photosynthetic time and efficiency, seriously affecting grain yield and quality in wheat...Leaf senescence is normally the last stage of plant development. Early senescence of functional leaves significantly reduces the photosynthetic time and efficiency, seriously affecting grain yield and quality in wheat. Discovering genes responsible for early leaf senescence(els) are necessary for developing novel germplasms and cultivars with delayed leaf-senescence through molecular manipulation and marker assisted selection. In this study, we identified an early leaf senescence line M114 in a derivative of a wheat breeding population. Genetic analysis indicated that early leaf senescence in M114 is controlled by a single recessive gene, provisionally designated els1. By applying bulked segregant analysis and RNA-Seq(BSR-Seq), seven polymorphic markers linked to els1 were developed and the gene was located on chromosome arm 2 BS in a 1.5 c M genetic interval between markers WGGB303 and WGGB305. A co-segregating marker, WGGB302, provide a starting point for fine mapping and map-based cloning of els1.展开更多
The theoretical design method of enhanced sensitivity fiber grating （FBG） strain sensors was given, and moreover high qualified strain sensors were developed and fabricated, whose sensing properties were good for pr...The theoretical design method of enhanced sensitivity fiber grating （FBG） strain sensors was given, and moreover high qualified strain sensors were developed and fabricated, whose sensing properties were good for practical applications. The strain sensor with cylindrical shell encapsulation contained three tubular structures, due to the uneven surface structure, in the area of the strain concentration, improving the sensitivity. It could achieve the embedment strain measurement and surface measurement and had the advantages of the easy installation. The good agreement was obtained between the measurements and theoretical simulation results. After each calibration test, twenty-four FBG strain sensors and six FBG temperature compensation sensors have been installed on the undersurface of the box girder of Diaoshuiyan bridge in Yongtaiwen highway. Finally, we built up a long-term structure health system for the highway bridge.展开更多
Phase-field method,as a powerful and popular approach to predict the mesoscale microstructure evolution in various materials science,provides a bridge from atomic-scale methods to the macroscale and has been widely us...Phase-field method,as a powerful and popular approach to predict the mesoscale microstructure evolution in various materials science,provides a bridge from atomic-scale methods to the macroscale and has been widely used at an ever-increasing rate.This paper aims to briefly review the origin,basic idea,and development of phase-field models in a historical manner.The focus is placed on the classical and state-of-the-art applications in China,including liquid–solid,solid–solid,gas–solid,ferroelectrics/ferromagnetics phase transformation,and crack propagation-fracture.After introducing the academic activities in the phase-field community in China,some suggestions for the future development directions of phase-field method are finally mentioned.展开更多
Plant height,spike,leaf,stem and grain morphologies are key components of plant architecture and related to wheat yield.A wheat(Triticum aestivum L.)mutant,wpa1,displaying temperaturedependent pleiotropic developmenta...Plant height,spike,leaf,stem and grain morphologies are key components of plant architecture and related to wheat yield.A wheat(Triticum aestivum L.)mutant,wpa1,displaying temperaturedependent pleiotropic developmental anomalies,was isolated.The WPA1 gene,encoding a von Willebrand factor type A(vWA)domain protein,was located on chromosome arm 7DS and isolated by map-based cloning.The functionality of WPA1 was validated by multiple independent EMS-induced mutants and gene editing.Phylogenetic analysis revealed that WPA1 is monocotyledon-specific in higher plants.The identification of WPA1 provides opportunity to study the temperature regulated wheat development and grain yield.展开更多
Soil enzyme activities have been suggested as suitable indicators for the evaluation of metal contamination because they are susceptible to microbial changes caused by heavy metal stress and are strictly related to so...Soil enzyme activities have been suggested as suitable indicators for the evaluation of metal contamination because they are susceptible to microbial changes caused by heavy metal stress and are strictly related to soil nutrient cycles.However,there is a growing lack of recognition and summary of the historic advancements that use soil enzymology as the proposal of evaluation methods.Here,we review the most common methods of heavy metal pollution evaluation based on enzyme activities,which include single enzyme index,combined enzyme index,enzyme-based functional diversity index,microbiological stress index,and ecoenzymatic stoichiometry models.This review critically examines the advantages and disadvantages of these methods based on their execution complexity,performance,and ecological implications and gets a glimpse of avenues to come to improved future evaluation systems.Indices based on a single enzyme are variable and have no consistent response to soil heavy metals,and the following three composite indices are characterized by the loss of many critical microbial processes,which thus not conducive to reflect the effects of heavy metals on soil ecosystems.Considering the dexterity of ecoenzymatic stoichiometry methods in reflecting changes in soil functions under heavy metal stress,we propose that microbial metabolic limitations quantified by ecoenzymatic stoichiometry models could be promising indicators for enhancing the reality and acceptance of results and further improving the potential for actual utility in environmental decision-making.展开更多
Magnesium and magnesium alloy foils have great potential for application in battery anodes,electromagnetic shielding,optics and acoustics,and biology because of their excellent specific damping,internal dissipation co...Magnesium and magnesium alloy foils have great potential for application in battery anodes,electromagnetic shielding,optics and acoustics,and biology because of their excellent specific damping,internal dissipation coefficients,magnetic and electrical conductivities,as well as high theoretical specific capacity.However,magnesium alloys exhibit poor deformation ability due to their hexagonal close-packed crystal structure.Preparing magnesium and magnesium alloy foils with thicknesses of less than 0.1 mm is difficult because of surface oxidation and grain growth at high temperatures or severe anisotropy after cold rolling that leads to cracks.Numerous methods have been applied to prepare magnesium alloy foils.They include warm rolling,cold rolling,accumulative roll bonding,electric plastic rolling,and on-line heating rolling.Defects of magnesium and magnesium alloy foils during preparation,such as edge cracks and breakage,are important factors for consideration.Herein,the current status of the research on magnesium and magnesium alloy foils is summarized from the aspects of foil preparation,defect control,performance characterization,and application prospects.The advantages and disadvantages of different preparation methods and defect(edge cracks and breakage)mechanisms in the preparation of foils are identified.展开更多
In present study,a novel organic depressant N-thiourea-maleamic acid(TMA)was synthesized and applied as a galena depressant in the flotation separation of Mo-Pb ores.The depression behaviors of TMA were tested through...In present study,a novel organic depressant N-thiourea-maleamic acid(TMA)was synthesized and applied as a galena depressant in the flotation separation of Mo-Pb ores.The depression behaviors of TMA were tested through flotation experiments.A wider separation window for single minerals over 90.0%was obtained at 30.0 mg/L TMA,confirming that TMA could depress galena significantly,while effected molybdenite floatability slightly.An effective separation was obtained for artificially mixed minerals and actual Mo-Pb ores.The adsorption mechanism on galena was revealed by UV-Vis spectra,zeta potential tests,Fourier transform infrared spectroscopy(FT-IR)analysis,contact angle tests and X-ray photoelectron spectroscopy(XPS)analysis.The zeta potentials of galena became more negative and the contact angle of galena dropped from initial 74.36°to 57.8°with 30.0 mg/L TMA depressant,inferring that TMA had adsorbed on galena surface.The analysis results of UV-Vis spectra,FT-IR and XPS gave further evidence that TMA might chemisorb on galena surface via Pb sites on galena surface and the thiourea group in TMA molecular structure,while the carboxyl group played a role of hydrophilicity.展开更多
Based on the principle of super-symmetric lens with quadratic phase gradient transformation, combined with the principle of digital coding of metasurface, we propose a wide-angle coded metalens for focusing control in...Based on the principle of super-symmetric lens with quadratic phase gradient transformation, combined with the principle of digital coding of metasurface, we propose a wide-angle coded metalens for focusing control in two-dimensional space. This metalens achieves focus shift in the x-direction by changing the oblique incidence angle of the incident wave,and focus control in the y-direction by combining with the convolution principle of the digitally coded metasurface to achieve flexible control of light focusing in the two-dimensional plane. The metasurface unit is mainly composed of threelayer of metal structure and two layers of medium, and the transmission phase is obtained by changing the middle layer of metal structure, which in turn obtains the required phase distribution of the metalens. The design of the metalens realizes the function of the lens with a large viewing angle at the x-polarized incidence, and realizes two-dimensional focus control. Experimentally, we prepared the designed coding metalens and tested the focus control function of the wide-angle coding metalens. The experimental results are in good agreement with the design results.展开更多
文摘A pot experiment and a sandy culture experiment grown with three vegetable crops of Chinese cabbage ( B. chinensis L., cv. Zao\|Shu 5), winter greens ( B.var.rosularis Tsen et Lee, cv. Shang\|Hai\|Qing) and celery ( A.graveolens L. var. dulce DC., cv. Qing\|Qin) were conducted, respectively. The initial soil and four incubated soils with different extractable Cd (0.15, 0.89, 1.38, 1.84 and 2.30 mg Cd/kg soil) were used for the pot experiment. Five treatments were designed (0, 0.0625, 0.125, 0.250 and 0.500 mg Cd/L) in nutrient solution in the sandy culture experiment. Each treatment in pot and sandy culture experiments was trireplicated. The objectives of the study were to examine Cd accumulation in edible parts of selected vegetable crops, its correlation with Cd concentrations in vegetable garden soil or in nutrient solution, and evaluate the criteria of Cd pollution in vegetable garden soil and in nutrient solution based on the hygienic limit of Cd in vegetables. Cadmium concentrations in edible parts of the three selected vegetable crops were as follows: 0.01—0.15 mg/kg fresh weight for Chinese cabbage, 0.02—0.17 mg/kg fresh weight for winter greens, and 0.02—0.24 mg/kg fresh weight for celery in the pot experiment, and 0.1—0.4 mg/kg fresh weight for Chinese cabbage, 0.1—1.4 mg/kg fresh weight for winter greens, and 0.05—0.5 mg/kg fresh weight for celery in the pot experiment(except no\|Cd treatment). The order of the three test vegetable crops for cadmium accumulation in the edible parts was celery>winter greens>Chinese cabbage in both the pot experiment and the sandy culture experiment. Cadmium accumulation in edible parts or roots of the vegetable crops increased with increasing of cadmium concentration in the medium(soil or nutrient solution). And cadmium concentrations in edible parts of the test vegetable crops were significantly linearly related to the Cd levels in the growth media (soil and nutrient solution). Based on the regression equations established and the limit of cad
文摘Dear Editor,Base editors(BEs)based on the CRISPR/Cas9 system,including cytosine base editors and adenine base editors,which can efficiently perform four transition mutations(C·G-to-T·A and A·T-to-G·C),have been well studied and widely used to produce base mutations in a variety of organisms,including in plants such as rice.
文摘This paper explores the capability of the“surface-protected etching”process for the creation of rattle-type SiO_(2)@void@SiO_(2) colloidal structures featuring a mesoporous silica shell and a mesoporous movable silica core.The surface-protected etching process involves stabilization of the particle surface using a polymer ligand,and then selective etching of the interior to form hollow structures.In this paper,this strategy has been extended to the formation of rattle-like structures by etching SiO_(2)@SiO_(2) core shell particles which are synthesized by a two-step sol gel process.The key is to introduce a protecting polymer of polyvinylpyrrolidone(PVP)to the surface of both core and shell in order to tailor their relative stability against chemical etching.Upon reacting with NaOH,the outer layer silica becomes a hollow shell as only the surface layer is protected by PVP and the interior is removed,while the core remains its original size thanks to the protection of PVP on its surface.This process can be carried out at room temperature without the need of additional templates or complicated heterogeneous coating procedures.The etching process also results in the rattle-type colloids having mesoscale pores with two distinct average sizes.In our demonstration of a model drug delivery process,such mesoporous structures show an interesting two-step elution profile which is believed to be related to the unique porous rattle structures.
基金This paper was partially supported by the Natural Science Foundation of China under Grant No. 51605348, the Natural Science Foundation of Hubei province under Grants No. 2016CFB116, and the Project of China Postdoctoral Science Foundation under Grant No. 2015M572208.
文摘During last decades, sensor elements based on the fiber Bragg grating （FBG） have been widely studied and developed due to the advantages of immunity to electromagnetic interference, compact size, high precision, and so on. The FBG itself is sensitive to axial strain and temperature variation directly and can indirectly measure these complex physical parameters, such as pressure, displacement, and vibration, by using some specially designed elastic structures to convert them into the axial strain of the FBG. Whether the FBG is fixed on the measured object to measure the strain directly or fixed on an elastic structure body to measure other physical quantities, these types of FBGs could be collectively called as strain sensing FBGs. The packaging of the FBG has important influence on FBG characteristics that directly affect the measurement accuracy, such as strain transfer, temperature characteristic, and spectral shape. This paper summarizes the packaging methods and corresponding temperature compensation methods of the currently reported strain sensing FBGs, focusing especially on fully pasted FBG, pre-stretched FBG with double-end fixed, and metallic packaging. Furthermore, the advantages and drawbacks of different packaging methods have been analyzed, which can provide a reference for future researches.
基金supported by the National Key Research and Development Program of China (2017YFD0101004)the Science and Technology Service Network Initiative of Chinese Academy of Sciences (KFJ-STS-ZDTP-024)
文摘Stripe rust and powdery mildew are both devastating diseases for durum and common wheat.Pyramiding of genes conferring resistance to one or more diseases in a single cultivar is an important breeding approach to provide broader spectra of resistances in wheat improvement. A new powdery mildew resistance gene originating from wild emmer(Triticum turgidum var.dicoccoides) backcrossed into common wheat(T. aestivum) line WE35 was identified. It conferred an intermediate level of resistance to Blumeria graminis f. sp. tritici isolate E09 at the seedling stage and a high level of resistance at the adult plant stage. Genetic analysis showed that the powdery mildew resistance in WE35 was controlled by a dominant gene designated Pm64. Bulked segregant analysis(BSA) and molecular mapping indicated that Pm64 was located in chromosome bin 2 BL4-0.50–0.89. Polymorphic markers were developed from the corresponding genomic regions of Chinese Spring wheat and wild emmer accession Zavitan to delimit Pm64 to a 0.55 cM genetic interval between markers WGGBH1364 and WGGBH612, corresponding to a 15 Mb genomic region on Chinese Spring and Zavitan 2 BL, respectively. The genetic linkage map of Pm64 is critical for fine mapping and cloning. Pm64 was completely linked in repulsion with stripe rust resistance gene Yr5. Analysis of a larger segregating population might identify a recombinant line with both genes as a valuable resource in breeding for resistance to powdery mildew and stripe rust.
基金This work was supported by Beijing Academy of Agriculture&Forestry Scien ces,Beijing,ChinaThe funding in eluded Innovative Team Con-struction Project of BAAFS(JNKYT201603)the Beijing Scholars Program(BSP041).
文摘The development of CRISPR/Cas9-mediated base editing has made genomic modification more efficient. However, selection of genetically modified cells from millions of treated cells, especially plant cells, is still challenging. In this study, an efficient surrogate reporter system based on a defective hygromycin resistance gene was established in rice to enrich base-edited cells. After step-by-step optimization, the Discriminated sgRNAs-based SurroGate system (DisSUGs) was established by artificially differentiating the editing abilities of a wild-type single guide RNA (sgRNA) targeting the surrogate reporter gene and an enhanced sgRNA targeting endogenous sites. The DisSUGs enhanced the efficiency of screening base-edited cells by 3- to 5-fold for a PmCDA1-based cytosine-to-tyrosine base editor (PCBE), and 2.5- to 6.5-fold for an adenine base editor (ABE) at endogenous targets. These targets showed editing efficiencies of <25% in the conventional systems. The DisSUGs greatly enhanced the frequency of homozygous substitutions and expanded the activity window slightly for both a PCBE and an ABE. Analyses of the total number of single-nucleotide variants from whole-genome sequencing revealed that, compared with the no-enrichment PCBE strategy, the DisSUGs did not alter the frequency of genome-wide sgRNA-independent off-target mutations, but slightly increased the frequency of target-dependent off-target mutations. Collectively, the DisSUGs developed in this study greatly enhances the efficiency of screening plant base-edited cells and will be a useful system in future applications.
基金the financial support of the project from "Key Laboratory Fund (5780011513) of Harbin Institute of Technology""University Basic Fund (5710011113) of Harbin Institute of Technology""the Fundamental Research Funds for the Central Universities" (Grant No. HIT. NSRIF. 20161)
文摘Microstructure and mechanical behavior of high volume content SiCp/7xxxAl composites have not been explored yet. Therefore, in the present work, 45 vol.% SiCp/7075Al composite has been prepared by pres- sure infiltration method. High density dislocations were found around SiC/Al interface in SiCp/7075Al composite after water-quenching and aging treatment. Fine dispersed nano-η＇ phases were observed after the aging treatment. Adverse to other SiCp/Al composites prepared by the pressure infiltration method, an interface layer was observed between SiC particles and AI matrix. Furthermore, high-resolution trans- mission electron microscopy （HRTEM） observation indicated that this interface layer was coherent/semi- coherent with that of the SiC particles. 45 vol.% SiCp/7075Al composite demonstrated high tensile strength （630 MPa） and micro-ductility. Compared to aged SiCp/2024Al composite, the aged SiCp/7075Al com- posite showed an increase of about 200% in the tensile strain and 90% in the tensile strength, respectively. It is speculated that nano-η＇ phases in the Al matrix significantly contributed to the strengthening effect while the interface layer between SiC and AI matrix might be beneficial to the strength and plasticity of SiCp/7075Al composite.
文摘Electron beam selective melting （EBSM） is a promising additive manufacturing （AM） technology. The EBSM process consists of three major procedures：（1） spreading a powder layer, （2） preheating to slightly sinter the powder, and （3） selectively melting the powder bed. The highly transient multi-physics phenomena involved in these procedures pose a significant challenge for in situ experimental observation and measurement. To advance the understanding of the physical mechanisms in each procedure, we leverage high- fidelity modeling and post-process experiments. The models resemble the actual fabrication procedures, including （1） a powder-spreading model using the discrete element method （DEM）, （2） a phase field （PF） model of powder sintering （solid-state sintering）, and （3） a powder-melting （liquid-state sintering） model using the finite volume method （FVM）. Comprehensive insights into all the major procedures are provided, which have rarely been reported. Preliminary simulation results （including powder particle packing within the powder bed, sintering neck formation between particles, and single-track defects） agree qualitatively with experiments, demonstrating the ability to understand the mechanisms and to guide the design and optimization of the experimental setup and manufacturing process.
文摘A brief review of recent progress in researches, productions and applications of full distributed fiber Raman photon sensors at China Jiliang University （CJLU） is presented. In order to improve the measurement distance, the accuracy, the space resolution, the ability of multi-parameter measurements, and the intelligence of full distributed fiber sensor systems, a new generation fiber sensor technology based on the optical fiber nonlinear scattering fusion principle is proposed. A series of new generation full distributed fiber sensors are investigated and designed, which consist of new generation ultra-long distance full distributed fiber Raman and Rayleigh scattering photon sensors integrated with a fiber Raman amplifier, auto-correction full distributed fiber Raman photon temperature sensors based on Raman correlation dual sources, full distributed fiber Raman photon temperature sensors based on a pulse coding source, full distributed fiber Raman photon temperature sensors using a fiber Raman wavelength shifter, a new type of Brillouin optical time domain analyzers （BOTDAs） integrated with a fiber Raman amplifier for replacing a fiber Brillouin amplifier, full distributed fiber Raman and Brillouin photon sensors integrated with a fiber Raman amplifier, and full distributed fiber Brillouin photon sensors integrated with a fiber Brillouin frequency shifter. The Internet of things is believed as one of candidates of the next technological revolution, which has driven hundreds of millions of class markets. Sensor networks are important components of the Internet of things. The full distributed optical fiber sensor network （Rayleigh, Raman, and Brillouin scattering） is a 3S （smart materials, smart structure, and smart skill） system, which is easy to construct smart fiber sensor networks. The distributed optical fiber sensor can be embedded in the power grids, railways, bridges, tunnels, roads, constructions, water supply systems, dams, oil and gas pipelines and other facilities, and can be integrate
文摘The Satellite Positioning and Orbit Determination System(SPODS)is a software package for GNSS positioning/orbit determination,developed by the Xi’an Research Institute of Surveying and Mapping.So far it has been able to analyse GPS data and has the capability of high precision GPS positioning and orbit determination.The underlying theory and the performance evaluation are briefly addressed in this paper.The experiments are carried out with GPS data collected from about 127 IGS stations during 4~10 January 2009.The results show that the RMS 1D difference is 1.1 cm between SPODS orbits and final IGS combined orbits,and that the repeatability of daily solutions of station coordinates is 1.5 mm for horizontal components,and 4.5 mm for vertical component,and that the consistency of ERP solutions with IGS final products is 0.025 mas,0.093 mas and 0.013 ms/d respectively for pole coordinates and LOD.
基金supported by the Natural Science Foundation of Anhui Province(070413119)and Clinical Testing and Diagnose Key Disciplines of Anhui Province
文摘Stromal cell-derived factor-1 and its receptor CXC chemokine receptor-4(CXCR4)have been implicated in breast cancer metastasis.A significant association between HER2 and CXCR4 expression has been observed in human breast tumor tissues,and overexpression of CXCR4 is essential for HER2-mediated tumor metastasis.Moreover,CXCR4 expression is low in normal breast tissues and high in malignant tumors,suggesting that a blockade of CXCR4 may limit tumor metastasis.The present study investigated the action of a synthetic antagonist 21-mer peptide derived from viral macrophage inflammatory protein Ⅱ against CXCR4(NT21MP)in inhibiting metastasis in vitro and in vivo.The results showed that chemotaxis of SKBR3 cells toward SDF-1αwas reduced by NT21MP in a dose-dependent manner(P<0.05).NT21MP inhibited tumor growth at 500μg/kg and in combination with Herceptin,the anti-HER2 antibody.The in vivo metastatic assay showed that NT21MP significantly inhibited pulmonary metastasis,and the number of metastatic tumor nodes on the surface of the lung was greatly decreased.Compared with the saline-treated control group,PCNA expression was dose-dependently decreased by NT21MP,the percentage of apoptotic cells was increased,and CXCR4 mRNA and protein expression were downregulated.In conclusion,NT21MP inhibits cellular proliferation,promotes apoptosis by downregulating CXCR4 expression,and suppresses the progression of primary and metastatic tumors.CXCR4 may be a useful therapeutic target for breast cancer,and NT21MP may serve as a potential target drug for the treatment of breast cancer metastasis.
基金financially supported by the National Key Research and Development Program of China (2016YFD0101802)
文摘Stripe rust(yellow rust), caused by Puccinia striiformis f. sp. tritici(PST),is one of the most devastating fungal diseases in common wheat(Triticum aestivum L.) in China and worldwide. Resistance breeding is the most effective strategy to control diseases in crop plants. Chinese wheat lines Mengmai 58 and Huaiyang 1 are highly resistant to PST race CYR34(V26) at the adult plant stage. To genetically map the underlying resistance genes we developed segregating populations by crossing Mengmai 58 and Huaiyang 1 with the susceptible cultivar Nongda 399. The stripe rust resistances in Mengmai 58 and Huaiyang 1 were both controlled by single dominant genes, provisionally designated YrMM58 and YrHY1, respectively. Bulked segregant RNA-Seq(BSR-Seq) analysis showed that YrMM58 and YrHY1 were located in the same distal ~16 Mb region on chromosome 2 AS.Comparative genomics analysis with the physical map of Aegilops tauschii proved useful for developing additional markers to saturate the genetic linkage map. YrMM58 and YrHY1 were mapped to the distal end of chromosome arm 2 AS, with the closest marker WGGB148 being 7.7 cM and 3.8 cM from the resistance gene, which was considered to be Yr17. These markers can be used in marker-assisted selection.
基金supported by the National Key Research and Development Program of China(2017YFD0101004)Science and Technology Service Network Initiative of Chinese Academy of Sciences(KFJ-STSZDTP-024)
文摘Leaf senescence is normally the last stage of plant development. Early senescence of functional leaves significantly reduces the photosynthetic time and efficiency, seriously affecting grain yield and quality in wheat. Discovering genes responsible for early leaf senescence(els) are necessary for developing novel germplasms and cultivars with delayed leaf-senescence through molecular manipulation and marker assisted selection. In this study, we identified an early leaf senescence line M114 in a derivative of a wheat breeding population. Genetic analysis indicated that early leaf senescence in M114 is controlled by a single recessive gene, provisionally designated els1. By applying bulked segregant analysis and RNA-Seq(BSR-Seq), seven polymorphic markers linked to els1 were developed and the gene was located on chromosome arm 2 BS in a 1.5 c M genetic interval between markers WGGB303 and WGGB305. A co-segregating marker, WGGB302, provide a starting point for fine mapping and map-based cloning of els1.
文摘The theoretical design method of enhanced sensitivity fiber grating （FBG） strain sensors was given, and moreover high qualified strain sensors were developed and fabricated, whose sensing properties were good for practical applications. The strain sensor with cylindrical shell encapsulation contained three tubular structures, due to the uneven surface structure, in the area of the strain concentration, improving the sensitivity. It could achieve the embedment strain measurement and surface measurement and had the advantages of the easy installation. The good agreement was obtained between the measurements and theoretical simulation results. After each calibration test, twenty-four FBG strain sensors and six FBG temperature compensation sensors have been installed on the undersurface of the box girder of Diaoshuiyan bridge in Yongtaiwen highway. Finally, we built up a long-term structure health system for the highway bridge.
基金the National Natural Science Foundation of China(Nos.52074246,52201146,52205429,52275390,U1904214)the National Defense Basic Scientific Research Program of China(No.JCKY2020408B002)+1 种基金the Key Research and Development Program of Shanxi Province(No.202102050201011)L.Z.acknowledges the Natural Science Foundation of Hunan Province for Distinguished Young Scholars(No.2021JJ10062).
文摘Phase-field method,as a powerful and popular approach to predict the mesoscale microstructure evolution in various materials science,provides a bridge from atomic-scale methods to the macroscale and has been widely used at an ever-increasing rate.This paper aims to briefly review the origin,basic idea,and development of phase-field models in a historical manner.The focus is placed on the classical and state-of-the-art applications in China,including liquid–solid,solid–solid,gas–solid,ferroelectrics/ferromagnetics phase transformation,and crack propagation-fracture.After introducing the academic activities in the phase-field community in China,some suggestions for the future development directions of phase-field method are finally mentioned.
基金supported by the Key Research and Development Program of Zhejiang(2024SSYS0099)the National Key Research and Development Program of China(2022YFD1200203)Key Research and Development Program of Hebei province(22326305D).
文摘Plant height,spike,leaf,stem and grain morphologies are key components of plant architecture and related to wheat yield.A wheat(Triticum aestivum L.)mutant,wpa1,displaying temperaturedependent pleiotropic developmental anomalies,was isolated.The WPA1 gene,encoding a von Willebrand factor type A(vWA)domain protein,was located on chromosome arm 7DS and isolated by map-based cloning.The functionality of WPA1 was validated by multiple independent EMS-induced mutants and gene editing.Phylogenetic analysis revealed that WPA1 is monocotyledon-specific in higher plants.The identification of WPA1 provides opportunity to study the temperature regulated wheat development and grain yield.
基金the National Natural Science Foundation of China(41977031)the Science Foundation for Distinguished Youth of Shaanxi Province(2020JC-31).
文摘Soil enzyme activities have been suggested as suitable indicators for the evaluation of metal contamination because they are susceptible to microbial changes caused by heavy metal stress and are strictly related to soil nutrient cycles.However,there is a growing lack of recognition and summary of the historic advancements that use soil enzymology as the proposal of evaluation methods.Here,we review the most common methods of heavy metal pollution evaluation based on enzyme activities,which include single enzyme index,combined enzyme index,enzyme-based functional diversity index,microbiological stress index,and ecoenzymatic stoichiometry models.This review critically examines the advantages and disadvantages of these methods based on their execution complexity,performance,and ecological implications and gets a glimpse of avenues to come to improved future evaluation systems.Indices based on a single enzyme are variable and have no consistent response to soil heavy metals,and the following three composite indices are characterized by the loss of many critical microbial processes,which thus not conducive to reflect the effects of heavy metals on soil ecosystems.Considering the dexterity of ecoenzymatic stoichiometry methods in reflecting changes in soil functions under heavy metal stress,we propose that microbial metabolic limitations quantified by ecoenzymatic stoichiometry models could be promising indicators for enhancing the reality and acceptance of results and further improving the potential for actual utility in environmental decision-making.
基金financially supported by the National Key Research and Development Program of China(Nos.2022 YFB3709300 and 2021YFB3701000)the National Natural Science Foundation of China(Nos.52271090 and 52071036)+1 种基金the Guangdong Major Project of Basic and Applied Basic Research(No.2020B0301030006)the Independent Research Project of State Key Laboratory of Mechanical Transmissions(Nos.SKLMT-ZZKT-2022Z01 and S KLMT-ZZKT-2022M12)。
文摘Magnesium and magnesium alloy foils have great potential for application in battery anodes,electromagnetic shielding,optics and acoustics,and biology because of their excellent specific damping,internal dissipation coefficients,magnetic and electrical conductivities,as well as high theoretical specific capacity.However,magnesium alloys exhibit poor deformation ability due to their hexagonal close-packed crystal structure.Preparing magnesium and magnesium alloy foils with thicknesses of less than 0.1 mm is difficult because of surface oxidation and grain growth at high temperatures or severe anisotropy after cold rolling that leads to cracks.Numerous methods have been applied to prepare magnesium alloy foils.They include warm rolling,cold rolling,accumulative roll bonding,electric plastic rolling,and on-line heating rolling.Defects of magnesium and magnesium alloy foils during preparation,such as edge cracks and breakage,are important factors for consideration.Herein,the current status of the research on magnesium and magnesium alloy foils is summarized from the aspects of foil preparation,defect control,performance characterization,and application prospects.The advantages and disadvantages of different preparation methods and defect(edge cracks and breakage)mechanisms in the preparation of foils are identified.
基金financial supports provided by National Key R&D Program of China(No.2017YFE0133100)National Natural Science Foundation of China(No.51974030)+1 种基金State Key Laboratory of Complex Nonferrous Metal Resources Clean Utilization(No.CNMRCUKF2102)the BGRIMM fund program(Grant No.02-1903).
文摘In present study,a novel organic depressant N-thiourea-maleamic acid(TMA)was synthesized and applied as a galena depressant in the flotation separation of Mo-Pb ores.The depression behaviors of TMA were tested through flotation experiments.A wider separation window for single minerals over 90.0%was obtained at 30.0 mg/L TMA,confirming that TMA could depress galena significantly,while effected molybdenite floatability slightly.An effective separation was obtained for artificially mixed minerals and actual Mo-Pb ores.The adsorption mechanism on galena was revealed by UV-Vis spectra,zeta potential tests,Fourier transform infrared spectroscopy(FT-IR)analysis,contact angle tests and X-ray photoelectron spectroscopy(XPS)analysis.The zeta potentials of galena became more negative and the contact angle of galena dropped from initial 74.36°to 57.8°with 30.0 mg/L TMA depressant,inferring that TMA had adsorbed on galena surface.The analysis results of UV-Vis spectra,FT-IR and XPS gave further evidence that TMA might chemisorb on galena surface via Pb sites on galena surface and the thiourea group in TMA molecular structure,while the carboxyl group played a role of hydrophilicity.
基金supported in part by the Science and technology innovation leading talent project of special support plan for high-level talents in Zhejiang Province(2021R52032)Natural Science Foundation of Zhejiang Province under grant No.LY22F050001+1 种基金Special project for professional degree postgraduates of Zhejiang Provincial Education Department(No.Y202353663,Y202353686)in part by the National Natural Science Foundation of China under grant No.62175224.China Jiliang University Basic Research Expenses.
文摘Based on the principle of super-symmetric lens with quadratic phase gradient transformation, combined with the principle of digital coding of metasurface, we propose a wide-angle coded metalens for focusing control in two-dimensional space. This metalens achieves focus shift in the x-direction by changing the oblique incidence angle of the incident wave,and focus control in the y-direction by combining with the convolution principle of the digitally coded metasurface to achieve flexible control of light focusing in the two-dimensional plane. The metasurface unit is mainly composed of threelayer of metal structure and two layers of medium, and the transmission phase is obtained by changing the middle layer of metal structure, which in turn obtains the required phase distribution of the metalens. The design of the metalens realizes the function of the lens with a large viewing angle at the x-polarized incidence, and realizes two-dimensional focus control. Experimentally, we prepared the designed coding metalens and tested the focus control function of the wide-angle coding metalens. The experimental results are in good agreement with the design results.
