Aim： To investigate whether chronic bacterial prostatitis might increase oxidative stress and oxidative damage in chronic bacterial prostatitis patients （CBPP）, and to explore its possible mechanism. Methods： Enro...Aim： To investigate whether chronic bacterial prostatitis might increase oxidative stress and oxidative damage in chronic bacterial prostatitis patients （CBPP）, and to explore its possible mechanism. Methods： Enrolled in a casecontrol study were 70 randomly sampled CBPP and 70 randomly sampled healthy adult volunteers （HAV）, on whom plasma nitric oxide （NO）, vitamin C （VC）, vitamin E （VE） and β-carotene （β-CAR） level, erythrocyte malondialdehyde （MDA） level, as well as erythrocyte superoxide dismutase （SOD）, catalase （CAT） and glutathione peroxidase （GPX） activities were determined by spectrophotometry. Results： Compared with the HAV group, values of plasma NO and erythrocyte MDA in the CBPP group were significantly increased （P 〈 0.001）; those of plasma VC, VE and β-CAR as well as erythrocyte SOD, CAT and GPX activities in the CBPP group were significantly decreased （P 〈 0.001）. Findings from partial correlation for the 70 CBPP showed that with prolonged course of disease, values of NO and MDA were gradually increased （P 〈 0.001）, and those of VC, VE, β-CAR, SOD, CAT and GPX were gradually decreased （P 〈 0.05- 0.001）. The findings from stepwise regression for the 70 CBPP suggested that the model was Y= -13.2077 ＋ 0.1894MDA ＋ 0.0415NO - 0.1999GPX, F = 18.2047, P 〈 0.001, r = 0.6729, P 〈 0.001. Conclusion： The findings suggest that there exist increased oxidative stress and oxidative damage induced by chronic bacterial prostatitis in the patients, and such phenomenon was closely related to the course of disease.展开更多
AIM To investigate the impact of cigarette smoking on the recurrence rate and recurrence-free survival in patients with hyperlipidemic acute pancreatitis(HLAP).METHODS A total of 863 patients were admitted to our hosp...AIM To investigate the impact of cigarette smoking on the recurrence rate and recurrence-free survival in patients with hyperlipidemic acute pancreatitis(HLAP).METHODS A total of 863 patients were admitted to our hospital for acute pancreatitis(AP) from January 2013 to March2016, of whom 88 diagnosed with HLAP were enrolled in this retrospective study. Demographic data, medical history, previous episodes of pancreatitis, consumption of alcohol and cigarettes, as well as biochemical and hematological data were carefully recorded for univariate and multivariate analyses. During followup, the information on current smoking status and recurrent AP was gathered. Recurrence-free survival(RFS) was calculated using the Kaplan-Meier method, and the differences between groups were compared using the log-rank test.RESULTS No significant differences were observed between the three groups in age or medical history of hyperlipidemia, fatty liver, diabetes mellitus, hypertension, or AP. The current smokers had a remarkably higher recurrence rate and a greater incidence of repeated episodes of AP(50.0% and 77.8%, respectively) than non-smokers(9.8% and 39.0%), and these two percentages were reduced to 9.1% and 36.4% for patients who gave up smoking. The median follow-up time was 13.5 mo and HLAP recurred after hospital discharge in 23(26.1%) patients. Multivariate analysis identified current smoking(HR = 6.3, P = 0.020) as an independent risk factor contributing to HLAP recurrence. Current smokers had significantly worse RFS than non-smokers(23 mo vs 42 mo), but no significant difference was documented between ex-smokers(34 mo) and non-smokers. The RFS was not significantly different between light and heavy smokers.CONCLUSION Smoking is associated with worse RFS and an increased rate of HLAP recurrence. Continued smoking correlates with a compromised survival and smoking cessation should be recommended.展开更多
The androgen receptor （AR） and its coregulators have important roles in the carcinogenesis of prostate cancer.p53 is an important tumour suppressor gerte,and the absence of a fundamental p53 response may predispose ...The androgen receptor （AR） and its coregulators have important roles in the carcinogenesis of prostate cancer.p53 is an important tumour suppressor gerte,and the absence of a fundamental p53 response may predispose to cancer. Transgelin,known as an ARA54-associated AR inhibitor,can suppress AR function in LNCaP cells. In addition to these effects,we aimed to elucidate the proapoptotic effects of the protein on LNCaP and its underlying mechanisms,especially the interaction between transgelin and p53. Cell counting,flow cytometric analysis and terminal deoxynucleotidyl transferase-dUTP nick-end labelling assays were applied to measure the proapoptotic effect of transgelin. Using western blotting of p53 and double immunofluorescence staining of p53 with transgelin,we show that transfection of transgelin results in increasing cytoplasmic translocation of p53 and upregulation of p53 expression. We also found an interaction between transgelin and p53 in vivo by mammalian two-hybrid and coimmunoprecipitation assays. The activation of the mitochondria-associated apoptosis pathway was observed in LNCaP cells after transfection with transgelin. These results are indicative of p53-mediated mitochondria-associated apoptotic effects of transgelin on LNCaP cells in addition to its known suppressive effects on the AR pathway.展开更多
Aim:To investigate whether chronic bacterial prostatitis might increase oxidative stress and oxidative damage in chronic bacterial prostatitis patients(CBPP),and to explore its possible mechanism.Methods:Enrolled in a...Aim:To investigate whether chronic bacterial prostatitis might increase oxidative stress and oxidative damage in chronic bacterial prostatitis patients(CBPP),and to explore its possible mechanism.Methods:Enrolled in a case- control study were 70 randomly sampled CBPP and 70 randomly sampled healthy adult volunteers(HAV),on whom plasma nitric oxide(NO),vitamin C(VC),vitamin E(VE)and β-carotene(β-CAR)level,erythrocyte malondialdehyde (MDA)level,as well as erythrocyte superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GPX) activities were determined by spectrophotometry.Results:Compared with the HAV group,values of plasma NO and erythrocyte MDA in the CBPP group were significantly increased(P<0,001);those of plasma VC,VE and β-CAR as well as erythrocyte SOD,CAT and GPX activities in the CBPP group were significantly decreased(P<0.001).Findings from partial correlation for the 70 CBPP showed that with prolonged course of disease,values of NO and MDA were gradually increased(P<0.001),and those of VC,VE,β-CAR,SOD,CAT and GPX were gradually decreased(P<0.05- 0.001).The findings from stepwise regression for the 70 CBPP suggested that the model was Y=-13.2077 + 0.1894MDA + 0.0415NO-0.1999GPX.F=18.2047,P<0.001,r=0.6729,P<0.001.Conclusion:The findings suggest that there exist increased oxidative stress and oxidative damage reduced by chronic bacterial prostatitis in the patients,and such phenomenon was closely related to the course of disease.展开更多
Objective:To study the correlation of glutathione S-transferase (GST) gene polymorphism with sperm quality, oxidative stress and apoptosis in patients with male infertility.Methods:Patients who were diagnosed with mal...Objective:To study the correlation of glutathione S-transferase (GST) gene polymorphism with sperm quality, oxidative stress and apoptosis in patients with male infertility.Methods:Patients who were diagnosed with male infertility combined with oligoasthenozoospermia for the first time in Guangdong Provincial Maternal and Child Health Care Hospital between July 2014 and June 2017 were enrolled in the infertility group and divided into GSTM1/T1[+/+], GSTM1/T1[+/-], GSTM1/T1[-/+] and GSTM1/T1[-/-] according to the polymorphism of GST gene GSTM1 and GSTT1 subtype deletion, and the semen quality parameters as well as the contents of oxidative stress indexes and apoptosis indexes in semen were determined.Results:The sperm concentration and sperm motility as well as the Nme5, PKC, ERK, TA and Bcl-2 levels in semen of patients with GSTM1/T1[+/-], GSTM1/T1[-/+] and GSTM1/T1[-/-] were significantly lower than those of patients with GSTM1/T1[+/+] whereas MDA, AOPP, AIF, Caspase-9 and Caspase-3 levels in semen were higher than those of patients with GSTM1/T1[+/+];the sperm concentration and sperm motility as well as the Nme5, PKC, ERK, TA and Bcl-2 levels in semen of patients with GSTM1/T1[-/-] were significantly lower than those of patients with GSTM1/T1[+/-] and GSTM1/T1[-/+] whereas MDA, AOPP, AIF, Caspase-9 and Caspase-3 levels in semen were higher than those of patients with GSTM1/T1[+/-] and GSTM1/T1[-/+].Conclusion:The deletion of GST gene GSTM1 and GSTT1 subtypes in patients with male infertility can lead to low sperm quality and activate the oxidative stress and apoptosis.展开更多
文摘Aim： To investigate whether chronic bacterial prostatitis might increase oxidative stress and oxidative damage in chronic bacterial prostatitis patients （CBPP）, and to explore its possible mechanism. Methods： Enrolled in a casecontrol study were 70 randomly sampled CBPP and 70 randomly sampled healthy adult volunteers （HAV）, on whom plasma nitric oxide （NO）, vitamin C （VC）, vitamin E （VE） and β-carotene （β-CAR） level, erythrocyte malondialdehyde （MDA） level, as well as erythrocyte superoxide dismutase （SOD）, catalase （CAT） and glutathione peroxidase （GPX） activities were determined by spectrophotometry. Results： Compared with the HAV group, values of plasma NO and erythrocyte MDA in the CBPP group were significantly increased （P 〈 0.001）; those of plasma VC, VE and β-CAR as well as erythrocyte SOD, CAT and GPX activities in the CBPP group were significantly decreased （P 〈 0.001）. Findings from partial correlation for the 70 CBPP showed that with prolonged course of disease, values of NO and MDA were gradually increased （P 〈 0.001）, and those of VC, VE, β-CAR, SOD, CAT and GPX were gradually decreased （P 〈 0.05- 0.001）. The findings from stepwise regression for the 70 CBPP suggested that the model was Y= -13.2077 ＋ 0.1894MDA ＋ 0.0415NO - 0.1999GPX, F = 18.2047, P 〈 0.001, r = 0.6729, P 〈 0.001. Conclusion： The findings suggest that there exist increased oxidative stress and oxidative damage induced by chronic bacterial prostatitis in the patients, and such phenomenon was closely related to the course of disease.
基金Supported by the National Natural Science Foundation of China,No.81501608
文摘AIM To investigate the impact of cigarette smoking on the recurrence rate and recurrence-free survival in patients with hyperlipidemic acute pancreatitis(HLAP).METHODS A total of 863 patients were admitted to our hospital for acute pancreatitis(AP) from January 2013 to March2016, of whom 88 diagnosed with HLAP were enrolled in this retrospective study. Demographic data, medical history, previous episodes of pancreatitis, consumption of alcohol and cigarettes, as well as biochemical and hematological data were carefully recorded for univariate and multivariate analyses. During followup, the information on current smoking status and recurrent AP was gathered. Recurrence-free survival(RFS) was calculated using the Kaplan-Meier method, and the differences between groups were compared using the log-rank test.RESULTS No significant differences were observed between the three groups in age or medical history of hyperlipidemia, fatty liver, diabetes mellitus, hypertension, or AP. The current smokers had a remarkably higher recurrence rate and a greater incidence of repeated episodes of AP(50.0% and 77.8%, respectively) than non-smokers(9.8% and 39.0%), and these two percentages were reduced to 9.1% and 36.4% for patients who gave up smoking. The median follow-up time was 13.5 mo and HLAP recurred after hospital discharge in 23(26.1%) patients. Multivariate analysis identified current smoking(HR = 6.3, P = 0.020) as an independent risk factor contributing to HLAP recurrence. Current smokers had significantly worse RFS than non-smokers(23 mo vs 42 mo), but no significant difference was documented between ex-smokers(34 mo) and non-smokers. The RFS was not significantly different between light and heavy smokers.CONCLUSION Smoking is associated with worse RFS and an increased rate of HLAP recurrence. Continued smoking correlates with a compromised survival and smoking cessation should be recommended.
文摘The androgen receptor （AR） and its coregulators have important roles in the carcinogenesis of prostate cancer.p53 is an important tumour suppressor gerte,and the absence of a fundamental p53 response may predispose to cancer. Transgelin,known as an ARA54-associated AR inhibitor,can suppress AR function in LNCaP cells. In addition to these effects,we aimed to elucidate the proapoptotic effects of the protein on LNCaP and its underlying mechanisms,especially the interaction between transgelin and p53. Cell counting,flow cytometric analysis and terminal deoxynucleotidyl transferase-dUTP nick-end labelling assays were applied to measure the proapoptotic effect of transgelin. Using western blotting of p53 and double immunofluorescence staining of p53 with transgelin,we show that transfection of transgelin results in increasing cytoplasmic translocation of p53 and upregulation of p53 expression. We also found an interaction between transgelin and p53 in vivo by mammalian two-hybrid and coimmunoprecipitation assays. The activation of the mitochondria-associated apoptosis pathway was observed in LNCaP cells after transfection with transgelin. These results are indicative of p53-mediated mitochondria-associated apoptotic effects of transgelin on LNCaP cells in addition to its known suppressive effects on the AR pathway.
文摘Aim:To investigate whether chronic bacterial prostatitis might increase oxidative stress and oxidative damage in chronic bacterial prostatitis patients(CBPP),and to explore its possible mechanism.Methods:Enrolled in a case- control study were 70 randomly sampled CBPP and 70 randomly sampled healthy adult volunteers(HAV),on whom plasma nitric oxide(NO),vitamin C(VC),vitamin E(VE)and β-carotene(β-CAR)level,erythrocyte malondialdehyde (MDA)level,as well as erythrocyte superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GPX) activities were determined by spectrophotometry.Results:Compared with the HAV group,values of plasma NO and erythrocyte MDA in the CBPP group were significantly increased(P<0,001);those of plasma VC,VE and β-CAR as well as erythrocyte SOD,CAT and GPX activities in the CBPP group were significantly decreased(P<0.001).Findings from partial correlation for the 70 CBPP showed that with prolonged course of disease,values of NO and MDA were gradually increased(P<0.001),and those of VC,VE,β-CAR,SOD,CAT and GPX were gradually decreased(P<0.05- 0.001).The findings from stepwise regression for the 70 CBPP suggested that the model was Y=-13.2077 + 0.1894MDA + 0.0415NO-0.1999GPX.F=18.2047,P<0.001,r=0.6729,P<0.001.Conclusion:The findings suggest that there exist increased oxidative stress and oxidative damage reduced by chronic bacterial prostatitis in the patients,and such phenomenon was closely related to the course of disease.
文摘Objective:To study the correlation of glutathione S-transferase (GST) gene polymorphism with sperm quality, oxidative stress and apoptosis in patients with male infertility.Methods:Patients who were diagnosed with male infertility combined with oligoasthenozoospermia for the first time in Guangdong Provincial Maternal and Child Health Care Hospital between July 2014 and June 2017 were enrolled in the infertility group and divided into GSTM1/T1[+/+], GSTM1/T1[+/-], GSTM1/T1[-/+] and GSTM1/T1[-/-] according to the polymorphism of GST gene GSTM1 and GSTT1 subtype deletion, and the semen quality parameters as well as the contents of oxidative stress indexes and apoptosis indexes in semen were determined.Results:The sperm concentration and sperm motility as well as the Nme5, PKC, ERK, TA and Bcl-2 levels in semen of patients with GSTM1/T1[+/-], GSTM1/T1[-/+] and GSTM1/T1[-/-] were significantly lower than those of patients with GSTM1/T1[+/+] whereas MDA, AOPP, AIF, Caspase-9 and Caspase-3 levels in semen were higher than those of patients with GSTM1/T1[+/+];the sperm concentration and sperm motility as well as the Nme5, PKC, ERK, TA and Bcl-2 levels in semen of patients with GSTM1/T1[-/-] were significantly lower than those of patients with GSTM1/T1[+/-] and GSTM1/T1[-/+] whereas MDA, AOPP, AIF, Caspase-9 and Caspase-3 levels in semen were higher than those of patients with GSTM1/T1[+/-] and GSTM1/T1[-/+].Conclusion:The deletion of GST gene GSTM1 and GSTT1 subtypes in patients with male infertility can lead to low sperm quality and activate the oxidative stress and apoptosis.
