Objective: To investigate the protective effects of the atomisation inhalation of edaravone on the lung tissues of rats with smoke inhalation injury. Methods: Forty male Sprague-Dawley (SD) rats were randomly divided ...Objective: To investigate the protective effects of the atomisation inhalation of edaravone on the lung tissues of rats with smoke inhalation injury. Methods: Forty male Sprague-Dawley (SD) rats were randomly divided into four groups of ten rats each: normal control group (group A), normal saline atomisation group (group B), edaravone aerosol group (group C) and edaravone atomisation prevention group (group D). Barring group A, the groups were used to create a model of severe smoke inhalation injury. However, before developing the model, group D rats were made to inhale edaravone (3.6 mg/mL) for 10 min. Six hours following smoke inhalation injury, abdominal artery blood samples were centrifuged, the lung tissue homogenate was prepared and carotid artery blood samples were used for blood gas analysis and oxygenation index (PaO<sub>2</sub>/FiO<sub>2</sub>) calculation. The levels of tumour necrosis factor alpha (TNF-α), interleukin (IL) 6 and IL-10 in serum and the levels of cysteine protease 3 (caspase-3), malondialdehyde (MDA), myeloperoxidase (MPO) and superoxide dismutase (SOD) in lung tissues were examined. The wet-dry ratio (W/D) and water content of the lung tissue were calculated, and the TUNEL method was used to determine the rate of lung tissue apoptosis in each group. Tissue specimens were obtained from the partial lung for histopathological examination. Results: Compared with those in group A, the water content of the lung tissue, the rate of lung tissue apoptosis, W/D and the caspase-3, TNF-α, IL-6, IL-10, MDA and MPO levels were significantly greater in other groups (PP< 0.05).<sup> </sup>Compared with those in group B, the levels of W/D, the water content of the lung tissue, the rate of lung tissue apoptosis and the levels of caspase-3, TNF-α, IL-6, MDA and MPO were significantly low (P and the levels of IL-10, SOD and PaO<sub>2</sub>/FiO<sub>2</sub> were significantly high in groups C and D (P The expression of the aforementioned factors was more evident in Group D (P < 0.05). Histopathological e展开更多
目的：探讨共情护理对实施甲状腺切除手术患者负性情绪及术后康复的影响。方法选取128例接受甲状腺切除术患者为研究对象，按照入院日期分为观察组62例与对照组66例，对照组给予常规护理模式，观察组在常规护理的基础上给予共情护理，比...目的：探讨共情护理对实施甲状腺切除手术患者负性情绪及术后康复的影响。方法选取128例接受甲状腺切除术患者为研究对象，按照入院日期分为观察组62例与对照组66例，对照组给予常规护理模式，观察组在常规护理的基础上给予共情护理，比较2组患者负性情绪、术后康复及满意度。结果干预后观察组 SAS 评分、SDS 评分均优于对照组[（35．09±4．01）分比（44．70±6．59）分，（36．55±4．12）分比（42．03±7．62）分，t ＝4．693、5．436，均 P ＜0．05]；观察组颈部肿胀、声音嘶哑、低血钙行发生率（1．61％、0．00％、3．23％）明显较低对照组（6．06％、6．06％、7．58％），差异均有统计学意义（χ2＝3．572、3．879、4．002，均 P ＜0．05）；观察组对护理人员满意度明显高于对照组（95．16％比78．79％），差异有统计学意义（χ2＝4．275，P ＜0．05）。结论共情护理能有效地缓解甲状腺切除手术患者的紧张情绪，减少血压、心率的波动，使患者以最佳的状态接受和配合手术治疗，改善术后康复。展开更多
为明确甘蓝型油菜花叶性状的遗传特点,开发与花叶性状连锁的分子标记。以甘蓝型油菜品系2205(圆叶)、1423(花叶)为亲本,构建了3个世代群体:F1、BC1和F2,探讨花叶性状的遗传规律;利用分子标记技术对花叶基因进行定位。结果表明,F1植株叶...为明确甘蓝型油菜花叶性状的遗传特点,开发与花叶性状连锁的分子标记。以甘蓝型油菜品系2205(圆叶)、1423(花叶)为亲本,构建了3个世代群体:F1、BC1和F2,探讨花叶性状的遗传规律;利用分子标记技术对花叶基因进行定位。结果表明,F1植株叶形表现为花叶,BC1(F1×2205)和F2中花叶与圆叶的植株分离比分别符合1∶1和3∶1,说明甘蓝型油菜的花叶性状受1对不完全显性基因控制。利用集团分离法(BSA)筛选637对SSR引物,共筛选到了3个与花叶基因紧密连锁的SSR标记:CB10079、BNGMS114和BNGMS385。连锁分析发现,这3个连锁标记均位于花叶基因的一侧,其中BNGMS114与花叶基因的遗传距离最近,其遗传距离为2.5 c M。将这3个连锁标记的序列与白菜基因组的序列进行比对,结果发现它们与白菜A10染色体的序列共线性较好,花叶基因位于A10染色体的15.70 Mb下游区段。上述标记的获得为油菜花叶性状的分子标记辅助选择育种以及花叶基因的克隆奠定理论基础。展开更多
文摘Objective: To investigate the protective effects of the atomisation inhalation of edaravone on the lung tissues of rats with smoke inhalation injury. Methods: Forty male Sprague-Dawley (SD) rats were randomly divided into four groups of ten rats each: normal control group (group A), normal saline atomisation group (group B), edaravone aerosol group (group C) and edaravone atomisation prevention group (group D). Barring group A, the groups were used to create a model of severe smoke inhalation injury. However, before developing the model, group D rats were made to inhale edaravone (3.6 mg/mL) for 10 min. Six hours following smoke inhalation injury, abdominal artery blood samples were centrifuged, the lung tissue homogenate was prepared and carotid artery blood samples were used for blood gas analysis and oxygenation index (PaO<sub>2</sub>/FiO<sub>2</sub>) calculation. The levels of tumour necrosis factor alpha (TNF-α), interleukin (IL) 6 and IL-10 in serum and the levels of cysteine protease 3 (caspase-3), malondialdehyde (MDA), myeloperoxidase (MPO) and superoxide dismutase (SOD) in lung tissues were examined. The wet-dry ratio (W/D) and water content of the lung tissue were calculated, and the TUNEL method was used to determine the rate of lung tissue apoptosis in each group. Tissue specimens were obtained from the partial lung for histopathological examination. Results: Compared with those in group A, the water content of the lung tissue, the rate of lung tissue apoptosis, W/D and the caspase-3, TNF-α, IL-6, IL-10, MDA and MPO levels were significantly greater in other groups (PP< 0.05).<sup> </sup>Compared with those in group B, the levels of W/D, the water content of the lung tissue, the rate of lung tissue apoptosis and the levels of caspase-3, TNF-α, IL-6, MDA and MPO were significantly low (P and the levels of IL-10, SOD and PaO<sub>2</sub>/FiO<sub>2</sub> were significantly high in groups C and D (P The expression of the aforementioned factors was more evident in Group D (P < 0.05). Histopathological e
文摘目的：探讨共情护理对实施甲状腺切除手术患者负性情绪及术后康复的影响。方法选取128例接受甲状腺切除术患者为研究对象，按照入院日期分为观察组62例与对照组66例，对照组给予常规护理模式，观察组在常规护理的基础上给予共情护理，比较2组患者负性情绪、术后康复及满意度。结果干预后观察组 SAS 评分、SDS 评分均优于对照组[（35．09±4．01）分比（44．70±6．59）分，（36．55±4．12）分比（42．03±7．62）分，t ＝4．693、5．436，均 P ＜0．05]；观察组颈部肿胀、声音嘶哑、低血钙行发生率（1．61％、0．00％、3．23％）明显较低对照组（6．06％、6．06％、7．58％），差异均有统计学意义（χ2＝3．572、3．879、4．002，均 P ＜0．05）；观察组对护理人员满意度明显高于对照组（95．16％比78．79％），差异有统计学意义（χ2＝4．275，P ＜0．05）。结论共情护理能有效地缓解甲状腺切除手术患者的紧张情绪，减少血压、心率的波动，使患者以最佳的状态接受和配合手术治疗，改善术后康复。
文摘为明确甘蓝型油菜花叶性状的遗传特点,开发与花叶性状连锁的分子标记。以甘蓝型油菜品系2205(圆叶)、1423(花叶)为亲本,构建了3个世代群体:F1、BC1和F2,探讨花叶性状的遗传规律;利用分子标记技术对花叶基因进行定位。结果表明,F1植株叶形表现为花叶,BC1(F1×2205)和F2中花叶与圆叶的植株分离比分别符合1∶1和3∶1,说明甘蓝型油菜的花叶性状受1对不完全显性基因控制。利用集团分离法(BSA)筛选637对SSR引物,共筛选到了3个与花叶基因紧密连锁的SSR标记:CB10079、BNGMS114和BNGMS385。连锁分析发现,这3个连锁标记均位于花叶基因的一侧,其中BNGMS114与花叶基因的遗传距离最近,其遗传距离为2.5 c M。将这3个连锁标记的序列与白菜基因组的序列进行比对,结果发现它们与白菜A10染色体的序列共线性较好,花叶基因位于A10染色体的15.70 Mb下游区段。上述标记的获得为油菜花叶性状的分子标记辅助选择育种以及花叶基因的克隆奠定理论基础。
