DELLA基因家族参与植物激素信号转导通路的调控,其中GAI(GA insensitive)mRNA还是植物体内长距离运输的信号分子。在全基因组范围内鉴定甘蓝(Brassica oleracea var. capitata) DELLA基因家族成员并分析mRNA运输特性,可为甘蓝DELLA基因...DELLA基因家族参与植物激素信号转导通路的调控,其中GAI(GA insensitive)mRNA还是植物体内长距离运输的信号分子。在全基因组范围内鉴定甘蓝(Brassica oleracea var. capitata) DELLA基因家族成员并分析mRNA运输特性,可为甘蓝DELLA基因家族的开发应用提供基础数据。本研究利用甘蓝基因组数据和转录组数据,在甘蓝中鉴定了5个DELLA基因家族成员(BoRGA1、BoRGA2、BoRGL1、BoRGL2和BoRGL3),但甘蓝基因组缺失了GAI基因。采用劈接法将甘蓝(自交系G27)接穗和菜心(Brassica campestris L. ssp. chinensis var.utilis Tsen et Lee)(四九菜心)砧木嫁接在一起,构建异源嫁接体。对砧木菜心花序轴和对应位置的实生苗菜心花序轴(对照)取样进行转录组测序。在甘蓝/菜心异源嫁接体的砧木菜心花序轴的转录组测序文库中分别鉴定到8、9、3、5、1个来自甘蓝BoRGA1、BoRGA2、BoRGL1、BoRGL2和BoRGL3的外源read。甘蓝DELLA家族基因mRNA运输并没有提高砧木菜心中DELLA家族基因的转录表达水平。相关性分析显示,甘蓝DELLA家族基因mRNA运输效率与其自身的序列和接穗甘蓝中的DELLA家族基因的转录表达水平相关。本研究为深入探究甘蓝DELLA家族基因mRNA运输的分子机制奠定了基础。展开更多
We demonstrate a simple method to measure electric field intensity by using doublet electromagnetically induced transparency(EIT) spectra of cold Rb Rydberg atoms, where the frequency of the coupling laser does not ne...We demonstrate a simple method to measure electric field intensity by using doublet electromagnetically induced transparency(EIT) spectra of cold Rb Rydberg atoms, where the frequency of the coupling laser does not need to be locked. Based on the Stark splitting of the Rb Rydberg state, 10D_(3/2), under electric fields and the corresponding calculated polarizabilities, the real electric field intensity is calculated using the difference in radio-frequency diffraction between two acousto-optic modulators, which acts as a frequency criterion that allows us to measure the electrical field without locking the coupling laser. The value measured by this simple method shows a good agreement with our previous work [Opt.Express 29 1558(2021)] where the frequency of the coupling laser needs to be locked with an additional EIT spectrum based on atom vapor and a proportional–integral–differential feedback circuit. Our presented method can also be extended to the measurement of electric field based on hot Rydberg atom vapor, which has application in industry.展开更多
文摘DELLA基因家族参与植物激素信号转导通路的调控,其中GAI(GA insensitive)mRNA还是植物体内长距离运输的信号分子。在全基因组范围内鉴定甘蓝(Brassica oleracea var. capitata) DELLA基因家族成员并分析mRNA运输特性,可为甘蓝DELLA基因家族的开发应用提供基础数据。本研究利用甘蓝基因组数据和转录组数据,在甘蓝中鉴定了5个DELLA基因家族成员(BoRGA1、BoRGA2、BoRGL1、BoRGL2和BoRGL3),但甘蓝基因组缺失了GAI基因。采用劈接法将甘蓝(自交系G27)接穗和菜心(Brassica campestris L. ssp. chinensis var.utilis Tsen et Lee)(四九菜心)砧木嫁接在一起,构建异源嫁接体。对砧木菜心花序轴和对应位置的实生苗菜心花序轴(对照)取样进行转录组测序。在甘蓝/菜心异源嫁接体的砧木菜心花序轴的转录组测序文库中分别鉴定到8、9、3、5、1个来自甘蓝BoRGA1、BoRGA2、BoRGL1、BoRGL2和BoRGL3的外源read。甘蓝DELLA家族基因mRNA运输并没有提高砧木菜心中DELLA家族基因的转录表达水平。相关性分析显示,甘蓝DELLA家族基因mRNA运输效率与其自身的序列和接穗甘蓝中的DELLA家族基因的转录表达水平相关。本研究为深入探究甘蓝DELLA家族基因mRNA运输的分子机制奠定了基础。
基金Project supported by the National Natural Science Foundation of China (Grant Nos. 12034012, 12074231, 12274272, and 61827824)Science and technology innovation plan of colleges and universities in Shanxi Province (Grant No. 2021L313)+2 种基金Science and Technology Project of State Grid (Grant No. 5700-202127198A-0-0-00)Fundamental Research Program of Shanxi Province (Grant No. 202203021222204)Taiyuan University of Science and Technology Scientific Research Initial Funding (Grant Nos. 20222008 and 20222132)。
文摘We demonstrate a simple method to measure electric field intensity by using doublet electromagnetically induced transparency(EIT) spectra of cold Rb Rydberg atoms, where the frequency of the coupling laser does not need to be locked. Based on the Stark splitting of the Rb Rydberg state, 10D_(3/2), under electric fields and the corresponding calculated polarizabilities, the real electric field intensity is calculated using the difference in radio-frequency diffraction between two acousto-optic modulators, which acts as a frequency criterion that allows us to measure the electrical field without locking the coupling laser. The value measured by this simple method shows a good agreement with our previous work [Opt.Express 29 1558(2021)] where the frequency of the coupling laser needs to be locked with an additional EIT spectrum based on atom vapor and a proportional–integral–differential feedback circuit. Our presented method can also be extended to the measurement of electric field based on hot Rydberg atom vapor, which has application in industry.
