A polyphenolic compound, 1,2,4,6-tetra-O-galloyl-β-D-glucose （1246TGG）, was isolated from the traditional Chinese medicine Phyllanthus emblica L. （Euphorbiaceae） and assayed for its potential as an anti-hepatitis...A polyphenolic compound, 1,2,4,6-tetra-O-galloyl-β-D-glucose （1246TGG）, was isolated from the traditional Chinese medicine Phyllanthus emblica L. （Euphorbiaceae） and assayed for its potential as an anti-hepatitis B virus （HBV） agent. The cytotoxicity of 1246TGG on HepG2.2.15 as well as HepG2 cells was determined by observing cytopathic effects, and the effects of 1246TGG on secretion of HBsAg and HBeAg in HepG2.2.15 cells were assayed by enzyme immunoassay. Results indicates that treatment with 1246TGG （6.25 ~tg/mL, 3.13 ~tg/mL）, reduced both HBsAg and HBeAg levels in culture supernatant, yet the inhibitory effects tend to decline with the assay time. This study provides a basis for further investigation of the anti-HBV activity and possible mechanism of action of 1246TGG展开更多
RNA interference (RNAi) is a process by which introduced small interfering RNA (siRNA) can cause the specific degradation of mRNA with identical sequences. The human herpes simplex virus type 1 (HSV-1) RR is composed ...RNA interference (RNAi) is a process by which introduced small interfering RNA (siRNA) can cause the specific degradation of mRNA with identical sequences. The human herpes simplex virus type 1 (HSV-1) RR is composed of two distinct homodimeric subunits encoded by UL39 and UL40,respectively. In this study,we applied siRNAs targeting the UL39 and UL40 genes of HSV-1. We showed that synthetic siRNA silenced effectively and specifically UL39 and UL40 mRNA expression and inhibited HSV-1 replication. Our work offers new possibilities for RNAi as a genetic tool for inhibition of HSV-1 replication.展开更多
Nucleoside analogues have been the mainstay of clinical treatment of herpes simplex virus 1 (HSV-1) infections since their development. However, the emergence of drug resistant strains has underlined the urgency of th...Nucleoside analogues have been the mainstay of clinical treatment of herpes simplex virus 1 (HSV-1) infections since their development. However, the emergence of drug resistant strains has underlined the urgency of the discovery of novel anti-HSV-1 drugs. Natural products, which provided many novel drug leads, are known to be an important source of anti-HSV-1 agents. Herein, we present an overview of natural products with anti-HSV-1 activities isolated from a variety of plants reported in recent years. Several different compounds, mainly belonging to the three groups of polysaccharides, polyphenols and terpenes, showed antiviral effects against HSV-1, indicating their potential to be promising anti-HSV-1 agents.展开更多
城市基础设施的全方位描述与信息化管理是建设新型智慧城市的重要基础。目前在城市基础设施建模与管理方面,以地图为模板的空间数据模型、传统的面向对象空间数据模型以及实时GIS数据模型不支持对时空对象组成结构、行为能力等要素的描...城市基础设施的全方位描述与信息化管理是建设新型智慧城市的重要基础。目前在城市基础设施建模与管理方面,以地图为模板的空间数据模型、传统的面向对象空间数据模型以及实时GIS数据模型不支持对时空对象组成结构、行为能力等要素的描述,无法全面、精细地表达设施对象信息,而计算机、物联网和地理信息系统(Geographic Information System,GIS)领域已有的城市基础设施管理方案忽略了对设施自身信息的管理,无法较好地支持设施对象及其产生数据的一体化管理。针对上述问题,首先通过扩展GBT30428.2-2013标准,完成了城市基础设施的分类与编码;然后基于多粒度时空对象数据模型(Multi-granularity Spatiotemporal Object Data Model,MGSTODM),提出了一种城市基础设施对象化描述模型,支持对城市基础设施信息的全方位描述与表达;在此基础上,提出一种城市基础设施对象化管理方法,设计了相应的技术架构,并研制了基于云存储的百万级城市基础设施对象化管理原型系统。试验结果表明,所提出的城市基础设施对象化描述模型与管理方法具备可行性、有效性和高效性。展开更多
基金Joint Funds of National Natural Science Foundation of China (U0632010)
文摘A polyphenolic compound, 1,2,4,6-tetra-O-galloyl-β-D-glucose （1246TGG）, was isolated from the traditional Chinese medicine Phyllanthus emblica L. （Euphorbiaceae） and assayed for its potential as an anti-hepatitis B virus （HBV） agent. The cytotoxicity of 1246TGG on HepG2.2.15 as well as HepG2 cells was determined by observing cytopathic effects, and the effects of 1246TGG on secretion of HBsAg and HBeAg in HepG2.2.15 cells were assayed by enzyme immunoassay. Results indicates that treatment with 1246TGG （6.25 ~tg/mL, 3.13 ~tg/mL）, reduced both HBsAg and HBeAg levels in culture supernatant, yet the inhibitory effects tend to decline with the assay time. This study provides a basis for further investigation of the anti-HBV activity and possible mechanism of action of 1246TGG
基金The Nation "863" Program of China(2006AA02A226)The Joint Funds of National Science Foundation of China (U0632010)+2 种基金The State KeyLaboratory of Phytochemistry and Plant Resources in West ChinaChinese Academy of Sciences (O807B11211, O807E21211)"211 grant of MOE"
文摘RNA interference (RNAi) is a process by which introduced small interfering RNA (siRNA) can cause the specific degradation of mRNA with identical sequences. The human herpes simplex virus type 1 (HSV-1) RR is composed of two distinct homodimeric subunits encoded by UL39 and UL40,respectively. In this study,we applied siRNAs targeting the UL39 and UL40 genes of HSV-1. We showed that synthetic siRNA silenced effectively and specifically UL39 and UL40 mRNA expression and inhibited HSV-1 replication. Our work offers new possibilities for RNAi as a genetic tool for inhibition of HSV-1 replication.
基金Joint funds of National Natural Science Foundation of China (U0632010)
文摘Nucleoside analogues have been the mainstay of clinical treatment of herpes simplex virus 1 (HSV-1) infections since their development. However, the emergence of drug resistant strains has underlined the urgency of the discovery of novel anti-HSV-1 drugs. Natural products, which provided many novel drug leads, are known to be an important source of anti-HSV-1 agents. Herein, we present an overview of natural products with anti-HSV-1 activities isolated from a variety of plants reported in recent years. Several different compounds, mainly belonging to the three groups of polysaccharides, polyphenols and terpenes, showed antiviral effects against HSV-1, indicating their potential to be promising anti-HSV-1 agents.
文摘城市基础设施的全方位描述与信息化管理是建设新型智慧城市的重要基础。目前在城市基础设施建模与管理方面,以地图为模板的空间数据模型、传统的面向对象空间数据模型以及实时GIS数据模型不支持对时空对象组成结构、行为能力等要素的描述,无法全面、精细地表达设施对象信息,而计算机、物联网和地理信息系统(Geographic Information System,GIS)领域已有的城市基础设施管理方案忽略了对设施自身信息的管理,无法较好地支持设施对象及其产生数据的一体化管理。针对上述问题,首先通过扩展GBT30428.2-2013标准,完成了城市基础设施的分类与编码;然后基于多粒度时空对象数据模型(Multi-granularity Spatiotemporal Object Data Model,MGSTODM),提出了一种城市基础设施对象化描述模型,支持对城市基础设施信息的全方位描述与表达;在此基础上,提出一种城市基础设施对象化管理方法,设计了相应的技术架构,并研制了基于云存储的百万级城市基础设施对象化管理原型系统。试验结果表明,所提出的城市基础设施对象化描述模型与管理方法具备可行性、有效性和高效性。
