Bamboo is one of the fastest growing plants among monocotyledonous species and is grown extensively in subtropical regions.Although bamboo has high economic value and produces much biomass quickly,gene functional rese...Bamboo is one of the fastest growing plants among monocotyledonous species and is grown extensively in subtropical regions.Although bamboo has high economic value and produces much biomass quickly,gene functional research is hindered by the low efficiency of genetic transformation in this species.We therefore explored the potential of a bamboo mosaic virus(BaMV)-mediated expression system to investigate genotype-phenotype associations.We determined that the sites between the triple gene block proteins(TGBps)and the coat protein(CP)of BaMV are the most efficient insertion sites for the expression of exogenous genes in both monopodial and sympodial bamboo species.Moreover,we validated this system by individually overexpressing the two endogenous genes ACE1 and DEC1,which resulted in the promotion and suppression of intemode elongation,respectively.In particular,this system was able to drive the expression of three 2A-linked betalain biosynthesis genes(more than 4 kb in length)to produce betalain,indicating that it has high cargo capacity and may provide the prerequisite basis for the development of a DNA-free bamboo genome editing platform in the future.Since BaMV can infect multiple bamboo species,we anticipate that the system described in this study will greatly contribute to gene function research and further promote the molecular breeding of bamboo.展开更多
To the Editor:Atherosclerosis is the main pathological feature of ischemic stroke,which remains a major cause of disability and death worldwide.[1]Vulnerable plaque,was characterized by ulceration,platelet activation,...To the Editor:Atherosclerosis is the main pathological feature of ischemic stroke,which remains a major cause of disability and death worldwide.[1]Vulnerable plaque,was characterized by ulceration,platelet activation,and thrombosis,potentially leading to emboli detachment and stroke.[2]Vascular smooth muscle cells(VSMCs),a major cellular component within lesions,can display pleiotropic phenotypes,including osteogenic or inammatory phenotypes,which may be harmful in maintaining advanced atherosclerotic plaque stability.[3]The modulation of the function of VSMCs,including proliferation,migration,and synthesizing the extracellular matrix(ECM)and matrix metalloproteinase(MMP),is thought to markedly affect plaque formation and vulnerability.[4]Therefore,it is of considerable signicance to drive harmful VSMCs toward VSMCs having benecial properties to strengthen plaque stability and retard plaque development.展开更多
Circular RNAs(circRNAs)are endogenous non-coding RNAs with covalently closed structures,which have important functions in plants.However,their biogenesis,degradation,and function upon treatment with gibberellins(GAs)a...Circular RNAs(circRNAs)are endogenous non-coding RNAs with covalently closed structures,which have important functions in plants.However,their biogenesis,degradation,and function upon treatment with gibberellins(GAs)and auxins(1-naphthaleneacetic acid,NAA)remain unknown.Here,we systematically identified and characterized the expression patterns,evolutionary conservation,genomic features,and internal structures of circRNAs using RNase R-treated libraries from moso bamboo(Phyllostachys edulis)seedlings.Moreover,we investigated the biogenesis of circRNAs dependent on both cis-and trans-regulation.We explored the function of circRNAs,including their roles in regulating microRNA(miRNA)-related genes and modulating the alternative splicing of their linear counterparts.Importantly,we developed a customized degradome sequencing approach to detect miRNA-mediated cleavage of circRNAs.Finally,we presented a comprehensive view of the participation of circRNAs in the regulation of hormone metabolism upon treatment of bamboo seedlings with GA and NAA.Collectively,our study provides insights into the biogenesis,function,and miRNA-mediated degradation of circRNAs in moso bamboo.展开更多
基金funded by the National Key Research and Development Program of China(2021YFD2200505)a grant from the National Natural Science Foundation of China(31971734)+1 种基金the Natural Science Foundation of Fujian Province(2021J02027)the Forestry Peak Discipline Construction Project of Fujian Agriculture and Forestry University(72202200205)。
文摘Bamboo is one of the fastest growing plants among monocotyledonous species and is grown extensively in subtropical regions.Although bamboo has high economic value and produces much biomass quickly,gene functional research is hindered by the low efficiency of genetic transformation in this species.We therefore explored the potential of a bamboo mosaic virus(BaMV)-mediated expression system to investigate genotype-phenotype associations.We determined that the sites between the triple gene block proteins(TGBps)and the coat protein(CP)of BaMV are the most efficient insertion sites for the expression of exogenous genes in both monopodial and sympodial bamboo species.Moreover,we validated this system by individually overexpressing the two endogenous genes ACE1 and DEC1,which resulted in the promotion and suppression of intemode elongation,respectively.In particular,this system was able to drive the expression of three 2A-linked betalain biosynthesis genes(more than 4 kb in length)to produce betalain,indicating that it has high cargo capacity and may provide the prerequisite basis for the development of a DNA-free bamboo genome editing platform in the future.Since BaMV can infect multiple bamboo species,we anticipate that the system described in this study will greatly contribute to gene function research and further promote the molecular breeding of bamboo.
基金Specifically Invited Professor of Oriental Scholar of Shanghai Colleges and Universities Tracking Program(No.GZ2016008)National Natural Science Foundation of China(No.81870347)
文摘To the Editor:Atherosclerosis is the main pathological feature of ischemic stroke,which remains a major cause of disability and death worldwide.[1]Vulnerable plaque,was characterized by ulceration,platelet activation,and thrombosis,potentially leading to emboli detachment and stroke.[2]Vascular smooth muscle cells(VSMCs),a major cellular component within lesions,can display pleiotropic phenotypes,including osteogenic or inammatory phenotypes,which may be harmful in maintaining advanced atherosclerotic plaque stability.[3]The modulation of the function of VSMCs,including proliferation,migration,and synthesizing the extracellular matrix(ECM)and matrix metalloproteinase(MMP),is thought to markedly affect plaque formation and vulnerability.[4]Therefore,it is of considerable signicance to drive harmful VSMCs toward VSMCs having benecial properties to strengthen plaque stability and retard plaque development.
基金supported by the National Natural Science Foundation of China(Grant Nos.31971734 and 31800566)the National Key R&D Program of China(Grant No.2021YFD2200505)+2 种基金the Distinguished Young Scholar Program of Fujian Agriculture and Forestry University(Grant No.xjq202017)the Scientific Research Foundation of Graduate School of Fujian Agriculture and Forestry University(Grant No.324-1122yb061)the Forestry Peak Discipline Construction Project of Fujian Agriculture and Forestry University(Grant No.72202200205),China。
文摘Circular RNAs(circRNAs)are endogenous non-coding RNAs with covalently closed structures,which have important functions in plants.However,their biogenesis,degradation,and function upon treatment with gibberellins(GAs)and auxins(1-naphthaleneacetic acid,NAA)remain unknown.Here,we systematically identified and characterized the expression patterns,evolutionary conservation,genomic features,and internal structures of circRNAs using RNase R-treated libraries from moso bamboo(Phyllostachys edulis)seedlings.Moreover,we investigated the biogenesis of circRNAs dependent on both cis-and trans-regulation.We explored the function of circRNAs,including their roles in regulating microRNA(miRNA)-related genes and modulating the alternative splicing of their linear counterparts.Importantly,we developed a customized degradome sequencing approach to detect miRNA-mediated cleavage of circRNAs.Finally,we presented a comprehensive view of the participation of circRNAs in the regulation of hormone metabolism upon treatment of bamboo seedlings with GA and NAA.Collectively,our study provides insights into the biogenesis,function,and miRNA-mediated degradation of circRNAs in moso bamboo.
