Myocardial ischemia–reperfusion injury(MIRI)is a major hindrance to the success of cardiac reperfusion therapy.Although increased neutrophil infiltration is a hallmark of MIRI,the subtypes and alterations of neutroph...Myocardial ischemia–reperfusion injury(MIRI)is a major hindrance to the success of cardiac reperfusion therapy.Although increased neutrophil infiltration is a hallmark of MIRI,the subtypes and alterations of neutrophils in this process remain unclear.Here,we performed single-cell sequencing of cardiac CD45^(+)cells isolated from the murine myocardium subjected to MIRI at six-time points.We identified diverse types of infiltrating immune cells and their dynamic changes during MIRI.Cardiac neutrophils showed the most immediate response and largest changes and featured with functionally heterogeneous subpopulations,including Ccl3^(hi)Neu and Ym-1^(hi)Neu,which were increased at 6 h and 1 d after reperfusion,respectively.Ym-1^(hi)Neu selectively expressed genes with protective effects and was,therefore,identified as a novel specific type of cardiac cell in the injured heart.Further analysis indicated that neutrophils and their subtypes orchestrated subsequent immune responses in the cardiac tissues,especially instructing the response of macrophages.The abundance of Ym-1^(hi)Neu was closely correlated with the therapeutic efficacy of MIRI when neutrophils were specifically targeted by anti-Lymphocyte antigen 6 complex locus G6D(Ly6G)or anti-Intercellular cell adhesion molecule-1(ICAM-1)neutralizing antibodies.In addition,a neutrophil subtype with the same phenotype as Ym-1^(hi)Neu was detected in clinical samples and correlated with prognosis.Ym-1 inhibition exacerbated myocardial injury,whereas Ym-1 supplementation significantly ameliorated injury in MIRI mice,which was attributed to the tilt of Ym-1 on the polarization of macrophages toward the repair phenotype in myocardial tissue.Overall,our findings reveal the antiinflammatory phenotype of Ym-1^(hi)Neu and highlight its critical role in myocardial protection during the early stages of MIRI.展开更多
The realization of quantum storage of spatial light field is of great significance to the construction of high-dimensional quantum repeater.In this paper,we experimentally realize the storage and retrieval of circular...The realization of quantum storage of spatial light field is of great significance to the construction of high-dimensional quantum repeater.In this paper,we experimentally realize the storage and retrieval of circular Airy beams(CABs)by using theΛ-type three-level energy system based on the electromagnetically induced transparency in a hot rubidium atomic vapor cell.The weak probe beam field is modulated with phase distribution of CABs by a spatial light modulator.We store the probe circular Airy beam(CAB)into the rubidium atomic vapor cell and retrieve it after the demanded delay.We quantitatively analyze the storage results and give corresponding theoretical explanations.Moreover,we investigate the autofocusing and self-healing effect of the retrieved CAB,which indicates that the properties and beam shape of CAB maintain well after storage.Our work will have potential applications in the storage of high-dimensional quantum information,and is also useful for improving the channel capacities of quantum internet.展开更多
Wen-Yi refers to an acute infectious disease that is highly contagious and can cause widespread epidemics.Also known as“Yi”or“Li”in the Chinese medicine(CM)literature,Wen-Yi diseases,such as corona virus disease 2...Wen-Yi refers to an acute infectious disease that is highly contagious and can cause widespread epidemics.Also known as“Yi”or“Li”in the Chinese medicine(CM)literature,Wen-Yi diseases,such as corona virus disease 2019(COVID-19),seasonal influenza,malaria,and tuberculosis,have imposed a significant disease burden globally.展开更多
The perfect hybrid vector vortex beam(PHVVB)with helical phase wavefront structure has aroused significant concern in recent years,as its beam waist does not expand with the topological charge(TC).In this work,we inve...The perfect hybrid vector vortex beam(PHVVB)with helical phase wavefront structure has aroused significant concern in recent years,as its beam waist does not expand with the topological charge(TC).In this work,we investigate the spatial quantum coherent modulation effect with PHVVB based on the atomic medium,and we observe the absorption characteristic of the PHVVB with different TCs under variant magnetic fields.We find that the transmission spectrum linewidth of PHVVB can be effectively maintained regardless of the TC.Still,the width of transmission peaks increases slightly as the beam size expands in hot atomic vapor.This distinctive quantum coherence phenomenon,demonstrated by the interaction of an atomic medium with a hybrid vector-structured beam,might be anticipated to open up new opportunities for quantum coherence modulation and accurate magnetic field measurement.展开更多
目的:分析复发/难治原发性中枢神经系统淋巴瘤(primary central nervous system lymphoma,PCNSL)患者临床特点并探讨其影响预后的因素,为临床诊疗提供依据。方法:选取复旦大学附属华山医院2006年10月至2015年8月确诊的64例复发/难治PCNS...目的:分析复发/难治原发性中枢神经系统淋巴瘤(primary central nervous system lymphoma,PCNSL)患者临床特点并探讨其影响预后的因素,为临床诊疗提供依据。方法:选取复旦大学附属华山医院2006年10月至2015年8月确诊的64例复发/难治PCNSL患者的病例资料、治疗方案、实验室辅助检查指标进行回顾性分析。采用Cox回归多因素分析。结果:单因素和多因素分析结果显示,首次无疾病进展生存期(progression-free survival of first time,PFS1)≤1年、Karnofsky评分(Karnofsky performance score,KPS)<70分为影响复发/难治PCNSL预后的独立危险因素。PFS1≥1年患者中位第二次无疾病进展生存期(median progression-free survival of secondtime,mPFS2)和中位第二次总生存时间(median overall survival of second time,mOS2)分别为19个月和21个月,而PFS1<1年患者mPFS2和mOS2分别为10个月和14个月。复发/难治时KPS评分≥70分患者与KPS评分<70分患者mPFS2分别为40个月和10个月,mOS2分别为43个月和12个月。另外,单因素分析首次复发/难治PCNSL患者选用含有大剂量甲氨蝶呤(high-dose methotrexate,HD-MTX)化疗方案的mPFS2为18个月,而选用不含HD-MTX化疗方案的mPFS2为10个月,差异具有统计学意义。多因素分析结果显示,挽救方案为影响患者PFS的相关因素;单因素分析结果显示,挽救方案含有HD-MTX与不含有HD-MTX组的mOS2分别为23个月和12个月,差异无统计学意义,考虑与样本量较小有关。结论:PFS1≤1年、KPS评分<70分是影响复发/难治PCNSL预后的独立危险因素。首次复发/难治PCNSL患者挽救治疗继续给予HD-MTX为基础的化疗方案可能会提高患者的远期疗效。展开更多
Vector beams with spiral phase and spatially varying polarization profiles have many applications from optical micromanipulation to materials processing. Here, we propose and demonstrate an atomic spatial mode extract...Vector beams with spiral phase and spatially varying polarization profiles have many applications from optical micromanipulation to materials processing. Here, we propose and demonstrate an atomic spatial mode extracting scheme for the vector beam based on polarization-dependent absorption in the atom vapor. By employing the linear polarization pump beam which induces polarization sensitive absorption in the atomic ensemble, a counter-propagated weak probe vector beam is extracted by spatial absorption, and extracted part still maintains the original polarization and the vortex phase.The topological charges of the extracted mode are verified by interfering with the Gaussian beam, and it can be found that the orbital angular momentum is conserved in the extracting process. Our work will have potential applications in non-destructive spatial mode identification, and is also useful for studying higher-dimensional quantum information based on atomic ensembles.展开更多
In many wild species, seeds are dormancy at maturity and will not germinate even under favorable environment conditions. Dormancy is a complex trail that is determined by many factors. Some studies have shown that col...In many wild species, seeds are dormancy at maturity and will not germinate even under favorable environment conditions. Dormancy is a complex trail that is determined by many factors. Some studies have shown that cold stratification and the application of gibberellic acid (GA) can break seed dormancy and promote seed germination. The present study investigated the causes of plant dormancy and the effect of cold stratification and different concentrations of exogenous GA<sub>3</sub> in regulating Cephalotaxus sinensis seed germination. Results showed that C. Sinensis seeds have good water permeability, which suggested that seed coats were not the main cause that inhibited the seed germination. There were germination inhibitions in all parts of seeds, and the order of inhibitory effect was: testa < endosperm (embryo), which indicated that existence of germination inhibitions was the main reason causing seed dormancy. Endogenous GAs and IAA (indole-3-acetic acid) content increased, while ABA (abscisic acid) content decreased over the experiments. ZR (zeatin riboside) content decreased in the early phase of cold stratification, but rebounded by the end of the experimental period. The changes of endogenous hormone indicated that GA, IAA and ZR played a positive role in seed germination, whereas ABA was associated with seed dormancy. Besides, the relative ratio of GA/ABA, IAA/ABA and ZR/ABA may play a more important role than their absolute level during the seed development.展开更多
基金supported by the National Natural Science Foundation of China(82022076,81974249,82070136,82104488,and 82305194)the Postdoctoral Science Foundation of China(2023M731222,and 2020T130040ZX)the Foundation of Hubei Key Laboratory of Biological Targeted Therapy(2023swbx021)。
文摘Myocardial ischemia–reperfusion injury(MIRI)is a major hindrance to the success of cardiac reperfusion therapy.Although increased neutrophil infiltration is a hallmark of MIRI,the subtypes and alterations of neutrophils in this process remain unclear.Here,we performed single-cell sequencing of cardiac CD45^(+)cells isolated from the murine myocardium subjected to MIRI at six-time points.We identified diverse types of infiltrating immune cells and their dynamic changes during MIRI.Cardiac neutrophils showed the most immediate response and largest changes and featured with functionally heterogeneous subpopulations,including Ccl3^(hi)Neu and Ym-1^(hi)Neu,which were increased at 6 h and 1 d after reperfusion,respectively.Ym-1^(hi)Neu selectively expressed genes with protective effects and was,therefore,identified as a novel specific type of cardiac cell in the injured heart.Further analysis indicated that neutrophils and their subtypes orchestrated subsequent immune responses in the cardiac tissues,especially instructing the response of macrophages.The abundance of Ym-1^(hi)Neu was closely correlated with the therapeutic efficacy of MIRI when neutrophils were specifically targeted by anti-Lymphocyte antigen 6 complex locus G6D(Ly6G)or anti-Intercellular cell adhesion molecule-1(ICAM-1)neutralizing antibodies.In addition,a neutrophil subtype with the same phenotype as Ym-1^(hi)Neu was detected in clinical samples and correlated with prognosis.Ym-1 inhibition exacerbated myocardial injury,whereas Ym-1 supplementation significantly ameliorated injury in MIRI mice,which was attributed to the tilt of Ym-1 on the polarization of macrophages toward the repair phenotype in myocardial tissue.Overall,our findings reveal the antiinflammatory phenotype of Ym-1^(hi)Neu and highlight its critical role in myocardial protection during the early stages of MIRI.
基金Project supported by the Youth Innovation Promotion Association of Chinese Academy of Sciences。
文摘The realization of quantum storage of spatial light field is of great significance to the construction of high-dimensional quantum repeater.In this paper,we experimentally realize the storage and retrieval of circular Airy beams(CABs)by using theΛ-type three-level energy system based on the electromagnetically induced transparency in a hot rubidium atomic vapor cell.The weak probe beam field is modulated with phase distribution of CABs by a spatial light modulator.We store the probe circular Airy beam(CAB)into the rubidium atomic vapor cell and retrieve it after the demanded delay.We quantitatively analyze the storage results and give corresponding theoretical explanations.Moreover,we investigate the autofocusing and self-healing effect of the retrieved CAB,which indicates that the properties and beam shape of CAB maintain well after storage.Our work will have potential applications in the storage of high-dimensional quantum information,and is also useful for improving the channel capacities of quantum internet.
基金supported by the China Academy of Chinese Medical Sciences Fund(2023007,ZZ17-YQ-036,z0849,z0832)the National Natural Science Foundation of China(82061138007,82274350)+1 种基金the CAMS Innovation Fund for Medical Sciences(CIFMS)(2021-1-I2M-035,2021-1-I2M-034,2021-CAMSJZ002)Guangdong Key Research and Development Project(2022B1111020005)。
文摘Wen-Yi refers to an acute infectious disease that is highly contagious and can cause widespread epidemics.Also known as“Yi”or“Li”in the Chinese medicine(CM)literature,Wen-Yi diseases,such as corona virus disease 2019(COVID-19),seasonal influenza,malaria,and tuberculosis,have imposed a significant disease burden globally.
基金Project supported by the Youth Innovation Promotion Association CASState Key Laboratory of Transient Optics and Photonics Open Topics (Grant No. SKLST202222)
文摘The perfect hybrid vector vortex beam(PHVVB)with helical phase wavefront structure has aroused significant concern in recent years,as its beam waist does not expand with the topological charge(TC).In this work,we investigate the spatial quantum coherent modulation effect with PHVVB based on the atomic medium,and we observe the absorption characteristic of the PHVVB with different TCs under variant magnetic fields.We find that the transmission spectrum linewidth of PHVVB can be effectively maintained regardless of the TC.Still,the width of transmission peaks increases slightly as the beam size expands in hot atomic vapor.This distinctive quantum coherence phenomenon,demonstrated by the interaction of an atomic medium with a hybrid vector-structured beam,might be anticipated to open up new opportunities for quantum coherence modulation and accurate magnetic field measurement.
基金supported by the National Key R&D Program of China(2021YFA1200201)Beijing Outstanding Young Scientists Projects(BJJWZYJH01201910005018)+1 种基金the National Natural Science Foundation of China(12174014,12372106)the Fundamental Research Funds for the Central Universities(YMF-22-L-808)。
文摘目的:分析复发/难治原发性中枢神经系统淋巴瘤(primary central nervous system lymphoma,PCNSL)患者临床特点并探讨其影响预后的因素,为临床诊疗提供依据。方法:选取复旦大学附属华山医院2006年10月至2015年8月确诊的64例复发/难治PCNSL患者的病例资料、治疗方案、实验室辅助检查指标进行回顾性分析。采用Cox回归多因素分析。结果:单因素和多因素分析结果显示,首次无疾病进展生存期(progression-free survival of first time,PFS1)≤1年、Karnofsky评分(Karnofsky performance score,KPS)<70分为影响复发/难治PCNSL预后的独立危险因素。PFS1≥1年患者中位第二次无疾病进展生存期(median progression-free survival of secondtime,mPFS2)和中位第二次总生存时间(median overall survival of second time,mOS2)分别为19个月和21个月,而PFS1<1年患者mPFS2和mOS2分别为10个月和14个月。复发/难治时KPS评分≥70分患者与KPS评分<70分患者mPFS2分别为40个月和10个月,mOS2分别为43个月和12个月。另外,单因素分析首次复发/难治PCNSL患者选用含有大剂量甲氨蝶呤(high-dose methotrexate,HD-MTX)化疗方案的mPFS2为18个月,而选用不含HD-MTX化疗方案的mPFS2为10个月,差异具有统计学意义。多因素分析结果显示,挽救方案为影响患者PFS的相关因素;单因素分析结果显示,挽救方案含有HD-MTX与不含有HD-MTX组的mOS2分别为23个月和12个月,差异无统计学意义,考虑与样本量较小有关。结论:PFS1≤1年、KPS评分<70分是影响复发/难治PCNSL预后的独立危险因素。首次复发/难治PCNSL患者挽救治疗继续给予HD-MTX为基础的化疗方案可能会提高患者的远期疗效。
文摘Vector beams with spiral phase and spatially varying polarization profiles have many applications from optical micromanipulation to materials processing. Here, we propose and demonstrate an atomic spatial mode extracting scheme for the vector beam based on polarization-dependent absorption in the atom vapor. By employing the linear polarization pump beam which induces polarization sensitive absorption in the atomic ensemble, a counter-propagated weak probe vector beam is extracted by spatial absorption, and extracted part still maintains the original polarization and the vortex phase.The topological charges of the extracted mode are verified by interfering with the Gaussian beam, and it can be found that the orbital angular momentum is conserved in the extracting process. Our work will have potential applications in non-destructive spatial mode identification, and is also useful for studying higher-dimensional quantum information based on atomic ensembles.
文摘In many wild species, seeds are dormancy at maturity and will not germinate even under favorable environment conditions. Dormancy is a complex trail that is determined by many factors. Some studies have shown that cold stratification and the application of gibberellic acid (GA) can break seed dormancy and promote seed germination. The present study investigated the causes of plant dormancy and the effect of cold stratification and different concentrations of exogenous GA<sub>3</sub> in regulating Cephalotaxus sinensis seed germination. Results showed that C. Sinensis seeds have good water permeability, which suggested that seed coats were not the main cause that inhibited the seed germination. There were germination inhibitions in all parts of seeds, and the order of inhibitory effect was: testa < endosperm (embryo), which indicated that existence of germination inhibitions was the main reason causing seed dormancy. Endogenous GAs and IAA (indole-3-acetic acid) content increased, while ABA (abscisic acid) content decreased over the experiments. ZR (zeatin riboside) content decreased in the early phase of cold stratification, but rebounded by the end of the experimental period. The changes of endogenous hormone indicated that GA, IAA and ZR played a positive role in seed germination, whereas ABA was associated with seed dormancy. Besides, the relative ratio of GA/ABA, IAA/ABA and ZR/ABA may play a more important role than their absolute level during the seed development.
