Aging is the major risk factor for many human diseases and degeneration.Thus,clinically effective medicine could delay the process of aging and aging-related diseases are desperately wanted.In traditional Chinese medi...Aging is the major risk factor for many human diseases and degeneration.Thus,clinically effective medicine could delay the process of aging and aging-related diseases are desperately wanted.In traditional Chinese medicine(TCM),some were claimed to slow down aging.Qingyangshen(Cynanchum otophyllum schneid)is such a TCM.Here,we assayed the longevity effect of compound Otophylloside B(Ot B),a C-21 steroidal glycoside isolated from Qingyangshen,in Caenorhabditis elegans,which is a popular model for aging research.Our results showed that Ot B could modestly extend the lifespan of C.elegans,delay the age-related decline of body movement and improve the stress resistance.Further investigating the molecular mechanism of lifespan extension effect revealed that Ot B could activate the FOXO transcription factor DAF-16.Ot B could not further extend the lifespan of long-lived mutant of insulin/IGF-1-like receptor(daf-2).In addition,Ot B also requires SIR-2.1 and CLK-1 which is an enzyme in ubiquinone synthesis,for lifespan extension.展开更多
Quantification of the impacts of environmental changes on runoff in the transitional area from the Tibetan Plateau to the Loess Plateau is of critical importance for regional water resources management.Trends and abru...Quantification of the impacts of environmental changes on runoff in the transitional area from the Tibetan Plateau to the Loess Plateau is of critical importance for regional water resources management.Trends and abrupt change points of the hydro-climatic variables in the Tao River Basin were investigated during 1956-2015.It also quantitatively separates the impacts of climate change and human activities on runoff change in the Tao River by using RCC-WBM model.Results indicate that temperature presented a significant rising trend(0.2℃per decade)while precipitation exhibited an insignificant decreasing trend(3.8 mm per decade)during 1956-2015.Recorded runoff in the Tao River decreased significantly with a magnitude of-13.7 mm per decade and abrupt changes in 1968 and 1986 were identified.Relative to the baseline period(1956-1968),runoff in the two anthropogenic disturbed periods of 1969-1986 and 1987-2015 decreased by 27.8 mm and 76.5 mm,respectively,which can be attributed to human activities(accounting for 69%)and climate change(accounting for 31%).Human activities are the principal drivers of runoff reduction in the Tao River Basin.However,the absolute influences on runoff reductions by the both drivers tend to increase,from 7.7 mm in 1969-1986 to 24.4 mm in 1987-2015 by climate change and from 20.2 mm to 52.2 mm by human activities.展开更多
Alzheimer’s disease(AD)is a major public health concern worldwide and the few drugs currently available only treat the symptoms.Hence,there is a strong need to find more effective anti-AD agents.Cynanchum otophyllum ...Alzheimer’s disease(AD)is a major public health concern worldwide and the few drugs currently available only treat the symptoms.Hence,there is a strong need to find more effective anti-AD agents.Cynanchum otophyllum is a traditional Chinese medicine for treating epilepsy,and otophylloside B(Ot B),isolated from C.otophyllum,is the essential active component.Having previously identified anti-aging effects of Ot B,we evaluated Ot B for AD prevention in C.elegant models of AD and found that Ot B extended lifespan,increased heat stress-resistance,delayed body paralysis,and increased the chemotaxis response.Collectively,these results indicated thatOt B protects against Aβtoxicity.Further mechanistic studies revealed that Ot B decreased Aβdeposition by decreasing the expression of Aβat the mRNA level.Genetic analyses showed that Ot B mediated its effects by increasing the activity of heat shock transcription factor(HSF)by upregulating the expression of hsf-1 and its target genes,hsp-12.6,hsp-16.2 and hsp-70.Ot B also increased the expression of sod-3 by partially activating DAF-16,while SKN-1 was not essential in Ot B-mediated protection against Aβtoxicity.展开更多
基金the Yunnan provincial government(20080A007)the 100 Talents Program of the Chinese Academy of Sciences,the State Key Laboratory of Phytochemistry and Plant Resources in West China,Kunming Institute of Botany(P2008-ZZ21 and T2009-KF05)。
文摘Aging is the major risk factor for many human diseases and degeneration.Thus,clinically effective medicine could delay the process of aging and aging-related diseases are desperately wanted.In traditional Chinese medicine(TCM),some were claimed to slow down aging.Qingyangshen(Cynanchum otophyllum schneid)is such a TCM.Here,we assayed the longevity effect of compound Otophylloside B(Ot B),a C-21 steroidal glycoside isolated from Qingyangshen,in Caenorhabditis elegans,which is a popular model for aging research.Our results showed that Ot B could modestly extend the lifespan of C.elegans,delay the age-related decline of body movement and improve the stress resistance.Further investigating the molecular mechanism of lifespan extension effect revealed that Ot B could activate the FOXO transcription factor DAF-16.Ot B could not further extend the lifespan of long-lived mutant of insulin/IGF-1-like receptor(daf-2).In addition,Ot B also requires SIR-2.1 and CLK-1 which is an enzyme in ubiquinone synthesis,for lifespan extension.
基金the National Key Research and Development Programs of China(2016YFA0601501)the National Natural Science Foundation of China(41830863,51879162,41601025)the Belt and Road Fund on Water and Sustainability of the State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering(2019).
文摘Quantification of the impacts of environmental changes on runoff in the transitional area from the Tibetan Plateau to the Loess Plateau is of critical importance for regional water resources management.Trends and abrupt change points of the hydro-climatic variables in the Tao River Basin were investigated during 1956-2015.It also quantitatively separates the impacts of climate change and human activities on runoff change in the Tao River by using RCC-WBM model.Results indicate that temperature presented a significant rising trend(0.2℃per decade)while precipitation exhibited an insignificant decreasing trend(3.8 mm per decade)during 1956-2015.Recorded runoff in the Tao River decreased significantly with a magnitude of-13.7 mm per decade and abrupt changes in 1968 and 1986 were identified.Relative to the baseline period(1956-1968),runoff in the two anthropogenic disturbed periods of 1969-1986 and 1987-2015 decreased by 27.8 mm and 76.5 mm,respectively,which can be attributed to human activities(accounting for 69%)and climate change(accounting for 31%).Human activities are the principal drivers of runoff reduction in the Tao River Basin.However,the absolute influences on runoff reductions by the both drivers tend to increase,from 7.7 mm in 1969-1986 to 24.4 mm in 1987-2015 by climate change and from 20.2 mm to 52.2 mm by human activities.
基金supported by the Natural Science Foundation of China(81671405,81370453)Natural Science Foundation of Yunnan province(2013FA045,2015FB172)Open Funds of Guangdong Key Laboratory of Marine Materia Mcdica(LMM2016-1).
文摘Alzheimer’s disease(AD)is a major public health concern worldwide and the few drugs currently available only treat the symptoms.Hence,there is a strong need to find more effective anti-AD agents.Cynanchum otophyllum is a traditional Chinese medicine for treating epilepsy,and otophylloside B(Ot B),isolated from C.otophyllum,is the essential active component.Having previously identified anti-aging effects of Ot B,we evaluated Ot B for AD prevention in C.elegant models of AD and found that Ot B extended lifespan,increased heat stress-resistance,delayed body paralysis,and increased the chemotaxis response.Collectively,these results indicated thatOt B protects against Aβtoxicity.Further mechanistic studies revealed that Ot B decreased Aβdeposition by decreasing the expression of Aβat the mRNA level.Genetic analyses showed that Ot B mediated its effects by increasing the activity of heat shock transcription factor(HSF)by upregulating the expression of hsf-1 and its target genes,hsp-12.6,hsp-16.2 and hsp-70.Ot B also increased the expression of sod-3 by partially activating DAF-16,while SKN-1 was not essential in Ot B-mediated protection against Aβtoxicity.
