AIM To investigate the prognostic value of preoperative fbri-nogen concentration （FIB） and D-dimer-fibrinogen ratio （DFR） in gastrointestinal stromal tumors （GISTs）.METHODS The purpose of this study was to retro...AIM To investigate the prognostic value of preoperative fbri-nogen concentration （FIB） and D-dimer-fibrinogen ratio （DFR） in gastrointestinal stromal tumors （GISTs）.METHODS The purpose of this study was to retrospectively ana-lyze 170 patients with GISTs who were admitted to our hospital from January 2010 to December 2015. The op-timal cutoff values of related parameters were estimated by receiver operating characteristic （ROC） curve analysis. The recurrence free survival （RFS） rate was evaluated using Kaplan-Meier curves. Univariate analysis and multivariate Cox regression models were used to analyze the prognostic factors of GISTs. The relationship between the FIB, D-dimer, DFR, platelet count （PLT）, and the clinicopathological features of GISTs was described by the chi-square test or nonparametric rank sum test （Mann-Whitney test）.RESULTS In ROC analysis, the optimal cutoff values of FIB, D-dimer, DFR, and PLT were 3.24 g/L, 1.24 mg/L, 0.354, and 197.5 （× 109/L）, respectively. Univariate analysis and the Kaplan-Meier survival curve showed that FIB, D-dimer, DFR, PLT,National Institutes of Health （NIH） risk category, tumor size, tumor location, and mitotic index were signifcantly relevant to the 3-year and 5-year survival rate of patients （ P 〈 0.05）. Cox multivariate regression analysis illustrated that FIB （RR： 0.108, 95%CI： 0.031-0.373）, DFR （RR： 0.319, 95%CI： 0.131-0.777）, and NIH risk category （ RR： 0.166, 95%CI： 0.047-0.589） were independent prognostic factors of the RFS rate （ P 〈 0. 05）. Moreover, FIB, D-dimer, DFR, and PLT were correlated with the clinical features of GISTs.CONCLUSIONFIB, D-dimer, DFR, and PLT are all related to the prognosis of GISTs. Moreover, FIB and DFR may be independent risk factors for predicting the prognosis of resectable GISTs.展开更多
BACKGROUND Over the past few years,research into the pathogenesis of colon cancer has progressed rapidly,and cuproptosis is an emerging mode of cellular apoptosis.Exploring the relationship between colon cancer and cu...BACKGROUND Over the past few years,research into the pathogenesis of colon cancer has progressed rapidly,and cuproptosis is an emerging mode of cellular apoptosis.Exploring the relationship between colon cancer and cuproptosis benefits in identifying novel biomarkers and even improving the outcome of the disease.AIM To look at the prognostic relationship between colon cancer and the genes associated with cuproptosis and the immune system in patients.The main purpose was to assess whether reasonable induction of these biomarkers reduces mortality among patients with colon cancers.METHOD Data obtained from The Cancer Genome Atlas and Gene Expression Omnibus and the Genotype-Tissue Expression were used in differential analysis to explore differential expression genes associated with cuproptosis and immune activation.The least absolute shrinkage and selection operator and Cox regression algorithm was applied to build a cuproptosis-and immune-related combination model,and the model was utilized for principal component analysis and survival analysis to observe the survival and prognosis of the patients.A series of statistically meaningful transcriptional analysis results demonstrated an intrinsic relationship between cuproptosis and the micro-environment of colon cancer.RESULTS Once prognostic characteristics were obtained,the CDKN2A and DLAT genes related to cuproptosis were strongly linked to colon cancer:The first was a risk factor,whereas the second was a protective factor.The finding of the validation analysis showed that the comprehensive model associated with cuproptosis and immunity was statistically significant.Within the component expressions,the expressions of HSPA1A,CDKN2A,and UCN3 differed markedly.Transcription analysis primarily reflects the differential activation of related immune cells and pathways.Furthermore,genes linked to immune checkpoint inhibitors were expressed differently between the subgroups,which may reveal the mechanism of worse prognosis and the different sensitivities of chemotherapy.CO展开更多
BACKGROUND The development of anaplastic lymphoma kinase(ALK)-tyrosine kinase inhibitors(TKIs)has remarkably improved the prognosis of patients with ALK-positive advanced non-small cell lung cancer(NSCLC).Alectinib,th...BACKGROUND The development of anaplastic lymphoma kinase(ALK)-tyrosine kinase inhibitors(TKIs)has remarkably improved the prognosis of patients with ALK-positive advanced non-small cell lung cancer(NSCLC).Alectinib,the second-generation ALK-TKI,has been approved as first-line treatment for advanced or metastatic NSCLC patients with ALK rearrangement.Neoadjuvant therapy can achieve tumor downstaging and eradicate occult lesions in patients with potentially resectable disease.Whether neoadjuvant alectinib can be a conversion therapy in ALK-positive advanced NSCLC patients remains unclear.CASE SUMMARY A 41-year-old man was pathologically diagnosed with locally advanced ALKpositive stage IIIB NSCLC.Alectinib was prescribed to induce tumor downstaging and facilitate the subsequent surgical resection.The tumor was successfully downstaged and pathological complete response was achieved.Left upper lobectomy with mediastinal lymphadenectomy was performed after tumor downstaging.The patient has continued to receive alectinib as adjuvant therapy during postoperative follow-up with a recurrence-free survival of 29 mo as of writing this report.CONCLUSION This case sheds light on the feasibility and safety of alectinib as a neoadjuvant treatment for stage IIIB NSCLC patients with ALK rearrangement.Its efficacy needs to be validated in prospective clinical trials.展开更多
Objective To investigate whether the connection of p27 Kip1 to S-phase kinase-associated protein 2 (Skp2) plays an oncogenic role in intraductal proliferative lesions of the breast. Methods Here we investigated the me...Objective To investigate whether the connection of p27 Kip1 to S-phase kinase-associated protein 2 (Skp2) plays an oncogenic role in intraductal proliferative lesions of the breast. Methods Here we investigated the mechanism involved in association of Skp2's degradation of p27 Kip1 with the breast carcinogenesis by immunohistochemical method through detection of Skp2 and p27 Kip1 protein levels in 120 paraffin-embedded tissues of intraductal proliferative lesions including usual ductal hyperplasia (UDH, n=30), atypical ductal hyperplasia (n=30), flat epithelial atypia (FEA, n=30), and ductal carcinoma in situ (DCIS, n=30). Moreover, the expression status of Skp2 and p27 Kip1 in 30 cases of the normal breast paraffin-embedded tissues were explored. Results The DCIS group was with the highest Skp2 level and the lowest p27 Kip1 level, and the UDH group was with the lowest Skp2 level and the highest p27 Kip1 level. Both Skp2 and p27 Kip1 levels in the DCIS group were significantly different from those in the UDH group (all P<0.01). The levels of Skp2 and p27 Kip1 in the FEA group were significantly different from both the DCIS and UDH groups (all P<0.05). p27 Kip1 was negatively correlated with Skp2 in both the UDH group (r=-0.629, P=0.026) and DCIS group (r=-0.893, P=0.000). Conclusion Overexpression of Skp2 might be the mechanism underlying p27 Kip1 over degradation.展开更多
Background: Although the prognostic impact of body mass index(BMI) in patients with non?metastatic naso?pharyngeal carcinoma(NPC) had been extensively studied, its effect among metastatic NPC patients remains unknown....Background: Although the prognostic impact of body mass index(BMI) in patients with non?metastatic naso?pharyngeal carcinoma(NPC) had been extensively studied, its effect among metastatic NPC patients remains unknown. The purpose of this study was to evaluate the prognostic effect of BMI in patients with metastatic NPC.Methods: We retrospectively studied 819 patients who were diagnosed with distant metastasis from NPC and received treatment between 1998 and 2007. The patients were divided into three subgroups according to the World Health Organization classifications for Asian populations: underweight(BMI <18.5 kg/m2), normal weight(BMI 18.5–22.9 kg/m2), and overweight/obese(BMI ≥23.0 kg/m2). The associations of BMI with overall survival(OS) and progression?free survival(PFS) were determined by Cox regression analysis.Results: Of the 819 patients, 168(20.5%) were underweight, 431(52.6%) were normal weight, and 220(26.9%) were overweight/obese. Multivariate analysis adjusted for covariates showed that overweight/obese patients had a longer OS than underweight patients [hazard ratio(HR), 0.64; 95% confidence interval(CI), 0.49–0.84] and normal weight patients(HR, 0.72; 95% CI, 0.57–0.90); no significant difference in PFS was observed among these three groups(P = 0.407). Moreover, in stratified analysis, no statistically significant differences in the effect of overweight/obese status among different subgroups were observed.Conclusion: For patients with metastatic NPC, overweight/obese status was associated with longer OS but not longer PFS compared with underweight or normal weight status.展开更多
In history,semiconductor-metal-semiconductor transistor(SMST)was proposed for frequency improvement.However,a general fabrication method is still missing due to the unsolved technological problem of deposition of a ge...In history,semiconductor-metal-semiconductor transistor(SMST)was proposed for frequency improvement.However,a general fabrication method is still missing due to the unsolved technological problem of deposition of a general crystalline semiconductor on metal,and a thinner metal base is also difficult to be fabricated with high quality.Recently,due to the atomic thickness of graphene,the concept of semiconductor-graphene-semiconductor transistor(SGST)has emerged which leads to the renaissance of SMST,however the experimental study is in its infancy.In this letter,SMST and SGST are fabricated using Si membrane transfer.It is found the common base current gain can be improved from about 0.5%in a Si-Au-Si transistor to about 1%in a Si-Gr-Ge one,and to above 10%in a Si-Gr-Si one,which is attributed to both the ultra-thin thickness and the quantum capacitance effect of graphene.展开更多
Distributed compressed sensing （DCS） is an emerging research field which exploits both intra-signal and inter-signal correlations. This paper focuses on the recovery of the sparse signals which can be modeled as joi...Distributed compressed sensing （DCS） is an emerging research field which exploits both intra-signal and inter-signal correlations. This paper focuses on the recovery of the sparse signals which can be modeled as joint sparsity model （JSM） 2 with different nonzero coefficients in the same location set. Smoothed L0 norm algorithm is utilized to convert a non-convex and intractable mixed L2,0 norm optimization problem into a solvable one. Compared with a series of single-measurement-vector problems, the proposed approach can obtain a better reconstruction performance by exploiting the inter-signal correlations. Simulation results show that our algorithm outperforms L1,1 norm optimization for both noiseless and noisy cases and is more robust against thermal noise compared with LI,2 recovery. Besides, with the help of the core concept of modified compressed sensing （CS） that utilizes partial known support as side information, we also extend this algorithm to decode correlated row sparse signals generated following JSM 1.展开更多
针对YOLOv3(you only look once version 3)对中小目标检测效果不理想的问题,提出改进算法DX-YOLO(densely ResneXt with YOLOv3)。首先对YOLOv3的特征提取网络Darknet-53进行改进,使用ResneXt残差模块替换原有残差模块,优化了卷积网络...针对YOLOv3(you only look once version 3)对中小目标检测效果不理想的问题,提出改进算法DX-YOLO(densely ResneXt with YOLOv3)。首先对YOLOv3的特征提取网络Darknet-53进行改进,使用ResneXt残差模块替换原有残差模块,优化了卷积网络结构;受DenseNet的启发,在Darknet-53中引入密集连接,实现了特征重用,提高了提取特征的效率;根据数据集的特点,利用K-means算法对数据集进行维度聚类,获得合适的预选框。在行人车辆数据集Udacity上进行实验,结果表明:DX-YOLO算法与YOLOv3相比,平均准确率(mean average precision,mAP)提升了3.42%;特别地,在中等目标和小目标上的平均精度(average precision,AP)分别提升了2.74%和5.98%。展开更多
文摘AIM To investigate the prognostic value of preoperative fbri-nogen concentration （FIB） and D-dimer-fibrinogen ratio （DFR） in gastrointestinal stromal tumors （GISTs）.METHODS The purpose of this study was to retrospectively ana-lyze 170 patients with GISTs who were admitted to our hospital from January 2010 to December 2015. The op-timal cutoff values of related parameters were estimated by receiver operating characteristic （ROC） curve analysis. The recurrence free survival （RFS） rate was evaluated using Kaplan-Meier curves. Univariate analysis and multivariate Cox regression models were used to analyze the prognostic factors of GISTs. The relationship between the FIB, D-dimer, DFR, platelet count （PLT）, and the clinicopathological features of GISTs was described by the chi-square test or nonparametric rank sum test （Mann-Whitney test）.RESULTS In ROC analysis, the optimal cutoff values of FIB, D-dimer, DFR, and PLT were 3.24 g/L, 1.24 mg/L, 0.354, and 197.5 （× 109/L）, respectively. Univariate analysis and the Kaplan-Meier survival curve showed that FIB, D-dimer, DFR, PLT,National Institutes of Health （NIH） risk category, tumor size, tumor location, and mitotic index were signifcantly relevant to the 3-year and 5-year survival rate of patients （ P 〈 0.05）. Cox multivariate regression analysis illustrated that FIB （RR： 0.108, 95%CI： 0.031-0.373）, DFR （RR： 0.319, 95%CI： 0.131-0.777）, and NIH risk category （ RR： 0.166, 95%CI： 0.047-0.589） were independent prognostic factors of the RFS rate （ P 〈 0. 05）. Moreover, FIB, D-dimer, DFR, and PLT were correlated with the clinical features of GISTs.CONCLUSIONFIB, D-dimer, DFR, and PLT are all related to the prognosis of GISTs. Moreover, FIB and DFR may be independent risk factors for predicting the prognosis of resectable GISTs.
文摘BACKGROUND Over the past few years,research into the pathogenesis of colon cancer has progressed rapidly,and cuproptosis is an emerging mode of cellular apoptosis.Exploring the relationship between colon cancer and cuproptosis benefits in identifying novel biomarkers and even improving the outcome of the disease.AIM To look at the prognostic relationship between colon cancer and the genes associated with cuproptosis and the immune system in patients.The main purpose was to assess whether reasonable induction of these biomarkers reduces mortality among patients with colon cancers.METHOD Data obtained from The Cancer Genome Atlas and Gene Expression Omnibus and the Genotype-Tissue Expression were used in differential analysis to explore differential expression genes associated with cuproptosis and immune activation.The least absolute shrinkage and selection operator and Cox regression algorithm was applied to build a cuproptosis-and immune-related combination model,and the model was utilized for principal component analysis and survival analysis to observe the survival and prognosis of the patients.A series of statistically meaningful transcriptional analysis results demonstrated an intrinsic relationship between cuproptosis and the micro-environment of colon cancer.RESULTS Once prognostic characteristics were obtained,the CDKN2A and DLAT genes related to cuproptosis were strongly linked to colon cancer:The first was a risk factor,whereas the second was a protective factor.The finding of the validation analysis showed that the comprehensive model associated with cuproptosis and immunity was statistically significant.Within the component expressions,the expressions of HSPA1A,CDKN2A,and UCN3 differed markedly.Transcription analysis primarily reflects the differential activation of related immune cells and pathways.Furthermore,genes linked to immune checkpoint inhibitors were expressed differently between the subgroups,which may reveal the mechanism of worse prognosis and the different sensitivities of chemotherapy.CO
文摘BACKGROUND The development of anaplastic lymphoma kinase(ALK)-tyrosine kinase inhibitors(TKIs)has remarkably improved the prognosis of patients with ALK-positive advanced non-small cell lung cancer(NSCLC).Alectinib,the second-generation ALK-TKI,has been approved as first-line treatment for advanced or metastatic NSCLC patients with ALK rearrangement.Neoadjuvant therapy can achieve tumor downstaging and eradicate occult lesions in patients with potentially resectable disease.Whether neoadjuvant alectinib can be a conversion therapy in ALK-positive advanced NSCLC patients remains unclear.CASE SUMMARY A 41-year-old man was pathologically diagnosed with locally advanced ALKpositive stage IIIB NSCLC.Alectinib was prescribed to induce tumor downstaging and facilitate the subsequent surgical resection.The tumor was successfully downstaged and pathological complete response was achieved.Left upper lobectomy with mediastinal lymphadenectomy was performed after tumor downstaging.The patient has continued to receive alectinib as adjuvant therapy during postoperative follow-up with a recurrence-free survival of 29 mo as of writing this report.CONCLUSION This case sheds light on the feasibility and safety of alectinib as a neoadjuvant treatment for stage IIIB NSCLC patients with ALK rearrangement.Its efficacy needs to be validated in prospective clinical trials.
基金Supported by the National Natural Science Foundation of China(30471967)research funding of Tianjin Cancer Institute&Hospitalof Tianjin Medical University
文摘Objective To investigate whether the connection of p27 Kip1 to S-phase kinase-associated protein 2 (Skp2) plays an oncogenic role in intraductal proliferative lesions of the breast. Methods Here we investigated the mechanism involved in association of Skp2's degradation of p27 Kip1 with the breast carcinogenesis by immunohistochemical method through detection of Skp2 and p27 Kip1 protein levels in 120 paraffin-embedded tissues of intraductal proliferative lesions including usual ductal hyperplasia (UDH, n=30), atypical ductal hyperplasia (n=30), flat epithelial atypia (FEA, n=30), and ductal carcinoma in situ (DCIS, n=30). Moreover, the expression status of Skp2 and p27 Kip1 in 30 cases of the normal breast paraffin-embedded tissues were explored. Results The DCIS group was with the highest Skp2 level and the lowest p27 Kip1 level, and the UDH group was with the lowest Skp2 level and the highest p27 Kip1 level. Both Skp2 and p27 Kip1 levels in the DCIS group were significantly different from those in the UDH group (all P<0.01). The levels of Skp2 and p27 Kip1 in the FEA group were significantly different from both the DCIS and UDH groups (all P<0.05). p27 Kip1 was negatively correlated with Skp2 in both the UDH group (r=-0.629, P=0.026) and DCIS group (r=-0.893, P=0.000). Conclusion Overexpression of Skp2 might be the mechanism underlying p27 Kip1 over degradation.
基金supported by the grant from the National High Technology Research and Development Program of China (863 Program No. 2012AA022701)
文摘Background: Although the prognostic impact of body mass index(BMI) in patients with non?metastatic naso?pharyngeal carcinoma(NPC) had been extensively studied, its effect among metastatic NPC patients remains unknown. The purpose of this study was to evaluate the prognostic effect of BMI in patients with metastatic NPC.Methods: We retrospectively studied 819 patients who were diagnosed with distant metastasis from NPC and received treatment between 1998 and 2007. The patients were divided into three subgroups according to the World Health Organization classifications for Asian populations: underweight(BMI <18.5 kg/m2), normal weight(BMI 18.5–22.9 kg/m2), and overweight/obese(BMI ≥23.0 kg/m2). The associations of BMI with overall survival(OS) and progression?free survival(PFS) were determined by Cox regression analysis.Results: Of the 819 patients, 168(20.5%) were underweight, 431(52.6%) were normal weight, and 220(26.9%) were overweight/obese. Multivariate analysis adjusted for covariates showed that overweight/obese patients had a longer OS than underweight patients [hazard ratio(HR), 0.64; 95% confidence interval(CI), 0.49–0.84] and normal weight patients(HR, 0.72; 95% CI, 0.57–0.90); no significant difference in PFS was observed among these three groups(P = 0.407). Moreover, in stratified analysis, no statistically significant differences in the effect of overweight/obese status among different subgroups were observed.Conclusion: For patients with metastatic NPC, overweight/obese status was associated with longer OS but not longer PFS compared with underweight or normal weight status.
基金supported by National Natural Science Foundation of China(Nos.62074150,61704175)Chinese Academy of Sciences(SYNL Young Talent Project 2020,SKLA-2019-03,Project Young Merit Scholars)。
文摘In history,semiconductor-metal-semiconductor transistor(SMST)was proposed for frequency improvement.However,a general fabrication method is still missing due to the unsolved technological problem of deposition of a general crystalline semiconductor on metal,and a thinner metal base is also difficult to be fabricated with high quality.Recently,due to the atomic thickness of graphene,the concept of semiconductor-graphene-semiconductor transistor(SGST)has emerged which leads to the renaissance of SMST,however the experimental study is in its infancy.In this letter,SMST and SGST are fabricated using Si membrane transfer.It is found the common base current gain can be improved from about 0.5%in a Si-Au-Si transistor to about 1%in a Si-Gr-Ge one,and to above 10%in a Si-Gr-Si one,which is attributed to both the ultra-thin thickness and the quantum capacitance effect of graphene.
基金supported by the National Natural Science Foundation of China(61201149)the 111 Project(B08004)the Fundamental Research Funds for the Central Universities
文摘Distributed compressed sensing （DCS） is an emerging research field which exploits both intra-signal and inter-signal correlations. This paper focuses on the recovery of the sparse signals which can be modeled as joint sparsity model （JSM） 2 with different nonzero coefficients in the same location set. Smoothed L0 norm algorithm is utilized to convert a non-convex and intractable mixed L2,0 norm optimization problem into a solvable one. Compared with a series of single-measurement-vector problems, the proposed approach can obtain a better reconstruction performance by exploiting the inter-signal correlations. Simulation results show that our algorithm outperforms L1,1 norm optimization for both noiseless and noisy cases and is more robust against thermal noise compared with LI,2 recovery. Besides, with the help of the core concept of modified compressed sensing （CS） that utilizes partial known support as side information, we also extend this algorithm to decode correlated row sparse signals generated following JSM 1.
文摘针对YOLOv3(you only look once version 3)对中小目标检测效果不理想的问题,提出改进算法DX-YOLO(densely ResneXt with YOLOv3)。首先对YOLOv3的特征提取网络Darknet-53进行改进,使用ResneXt残差模块替换原有残差模块,优化了卷积网络结构;受DenseNet的启发,在Darknet-53中引入密集连接,实现了特征重用,提高了提取特征的效率;根据数据集的特点,利用K-means算法对数据集进行维度聚类,获得合适的预选框。在行人车辆数据集Udacity上进行实验,结果表明:DX-YOLO算法与YOLOv3相比,平均准确率(mean average precision,mAP)提升了3.42%;特别地,在中等目标和小目标上的平均精度(average precision,AP)分别提升了2.74%和5.98%。
