Herein,the 2,4-dinitrophenyl functional group acting as the thiophenol reactive site was introduced into a carbazole-chalcone fluorophore to synthesize probe-CCF2,which could result in a remarkable increase in fluores...Herein,the 2,4-dinitrophenyl functional group acting as the thiophenol reactive site was introduced into a carbazole-chalcone fluorophore to synthesize probe-CCF2,which could result in a remarkable increase in fluorescence when reacting with thiophenols.The selectivity and accuracy of probe-CCF2 were investigated with thiophenols,hydrosulphide salt,aliphatic thiols,glutathione,cysteine,anions and metal cations.Probe-CCF2 exhibited a detection limit of 37 nmol/L(R^(2)=0.9951),a remarkable Stokes shift of approximately 130 nm,and a brief response time of 9 min with a remarkable increase in fluorescence of 90-fold.Probe-CCF2 was applied for thiophenol detection in water samples and imaging in living cells successfully,with high sensitivity and excellent selectivity.展开更多
The Coronavirus Disease 2019(COVID-19)highlights the importance of understanding and controlling the spread of the coronavirus between persons.We experimentally and numerically investigated an advanced engineering and...The Coronavirus Disease 2019(COVID-19)highlights the importance of understanding and controlling the spread of the coronavirus between persons.We experimentally and numerically investigated an advanced engineering and environmental method on controlling the transmission of airborne SARS-CoV-2-laden aerosols in the breathing microenvironment between two persons during interactive breathing process by combining the limited space air stability and a ventilation method.Experiments were carried out in a full-scale ventilated room with different limited space air stability conditions,i.e.,stable condition,neutral condition and unstable condition.Two real humans were involved to conducted normal breathing process in the room and the exhaled carbon dioxide was used as the surrogate of infectious airborne SARS-CoV-2-laden aerosols from respiratory activities.A correspondent numerical model was established to visualize the temperature field and contaminated field in the test room.Results show that the performance of a ventilation system on removing infectious airborne SARS-CoV-2-laden aerosols from the interpersonal breathing microenvironment is dependent on the limited space air stability conditions.Appropriate ventilation method should be implemented based on an evaluation of the air condition.It is recommended that total volume ventilation methods are suitable for unstable and neutral conditions and local ventilation methods are preferable for stable conditions.This study provides an insight into the transmission of airborne SARS-CoV-2-laden aerosols between persons in ventilated rooms with different limited space air stability conditions.Useful guidance has been provided to cope with COVID-19 in limited spaces.展开更多
基金supported by Natural Science Foundation of Zhejiang Province(LQ21C200002)Zhejiang Provincial Key Research and Development Program(2021CO2062,2022C02028)Key Research and Development Program of Ningbo(2021Z056,2022S163)
文摘Herein,the 2,4-dinitrophenyl functional group acting as the thiophenol reactive site was introduced into a carbazole-chalcone fluorophore to synthesize probe-CCF2,which could result in a remarkable increase in fluorescence when reacting with thiophenols.The selectivity and accuracy of probe-CCF2 were investigated with thiophenols,hydrosulphide salt,aliphatic thiols,glutathione,cysteine,anions and metal cations.Probe-CCF2 exhibited a detection limit of 37 nmol/L(R^(2)=0.9951),a remarkable Stokes shift of approximately 130 nm,and a brief response time of 9 min with a remarkable increase in fluorescence of 90-fold.Probe-CCF2 was applied for thiophenol detection in water samples and imaging in living cells successfully,with high sensitivity and excellent selectivity.
基金supported by the National Natural Science Foundation of China(No.51378186)the National Science and Technology Support Program(No.2015BAJ03B00)the China Scholarship Council(No.201806130150)。
文摘The Coronavirus Disease 2019(COVID-19)highlights the importance of understanding and controlling the spread of the coronavirus between persons.We experimentally and numerically investigated an advanced engineering and environmental method on controlling the transmission of airborne SARS-CoV-2-laden aerosols in the breathing microenvironment between two persons during interactive breathing process by combining the limited space air stability and a ventilation method.Experiments were carried out in a full-scale ventilated room with different limited space air stability conditions,i.e.,stable condition,neutral condition and unstable condition.Two real humans were involved to conducted normal breathing process in the room and the exhaled carbon dioxide was used as the surrogate of infectious airborne SARS-CoV-2-laden aerosols from respiratory activities.A correspondent numerical model was established to visualize the temperature field and contaminated field in the test room.Results show that the performance of a ventilation system on removing infectious airborne SARS-CoV-2-laden aerosols from the interpersonal breathing microenvironment is dependent on the limited space air stability conditions.Appropriate ventilation method should be implemented based on an evaluation of the air condition.It is recommended that total volume ventilation methods are suitable for unstable and neutral conditions and local ventilation methods are preferable for stable conditions.This study provides an insight into the transmission of airborne SARS-CoV-2-laden aerosols between persons in ventilated rooms with different limited space air stability conditions.Useful guidance has been provided to cope with COVID-19 in limited spaces.
