Hydroxyapatite(HA)bioceramic scaffolds were fabricated by using digital light processing(DLP)based additive manufacturing.Key issues on the HA bioceramic scaffolds,including dispersion,DLP fabrication,sintering,mechan...Hydroxyapatite(HA)bioceramic scaffolds were fabricated by using digital light processing(DLP)based additive manufacturing.Key issues on the HA bioceramic scaffolds,including dispersion,DLP fabrication,sintering,mechanical properties,and biocompatibility were discussed in detail.Firstly,the ffects of dispersant dosage,solid loading,and sintering temperature were studied.The optimal dispersant dosage,solid loading,and sintering temperature were 2wt%,50vol%,and 1250℃,respectively.Then,the mechanical properties and biocompatibility of the HA bioceramic scaffolds were investigated.The DLP-prepared porous HA bioceramic scaffold was found to exhibit excellent mechanical properties and degradation behavior.From this study,DLP technique shows good potential for manufacturing HA bioceramic scaffolds.展开更多
Mo_(2)Ga_(2)C is a new MAX phase with a stacking Ga-bilayer as well as possible unusual properties.To understand this unique MAX phase structure and promote possible future applications,the structure,chemical bonding,...Mo_(2)Ga_(2)C is a new MAX phase with a stacking Ga-bilayer as well as possible unusual properties.To understand this unique MAX phase structure and promote possible future applications,the structure,chemical bonding,and mechanical and thermodynamic properties of Mo_(2)Ga_(2)C were investigated by first-principles.Using the“bond stiffness”model,the strongest covalent bonding(1162 GPa)was formed between Mo and C atoms in Mo_(2)Ga_(2)C,while the weakest Ga–Ga(389 GPa)bonding was formed between two Ga-atomic layers,different from other typical MAX phases.The ratio of the bond stiffness of the weakest bond to the strongest bond(0.33)was lower than 1/2,indicating the high damage tolerance and fracture toughness of Mo_(2)Ga_(2)C,which was confirmed by indentation without any cracks.The high-temperature heat capacity and thermal expansion of Mo_(2)Ga_(2)C were calculated in the framework of quasi-harmonic approximation from 0 to 1300 K.Because of the metal-like electronic structure,the electronic excitation contribution became more significant with increasing temperature above 300 K.展开更多
Neonatal hypoxic-ischemic brain injury is the main cause of hypoxic-ischemic encephalopathy and cerebral palsy.Currently,there are few effective clinical treatments for neonatal hypoxic-ischemic brain injury.Here,we i...Neonatal hypoxic-ischemic brain injury is the main cause of hypoxic-ischemic encephalopathy and cerebral palsy.Currently,there are few effective clinical treatments for neonatal hypoxic-ischemic brain injury.Here,we investigated the neuroprotective and molecular mechanisms of exogenous nicotinamide adenine dinucleotide,which can protect against hypoxic injury in adulthood,in a mouse model of neonatal hypoxic-ischemic brain injury.In this study,nicotinamide adenine dinucleotide(5 mg/kg)was intraperitoneally administered 30 minutes befo re surgery and every 24 hours thereafter.The results showed that nicotinamide adenine dinucleotide treatment improved body weight,brain structure,adenosine triphosphate levels,oxidative damage,neurobehavioral test outcomes,and seizure threshold in experimental mice.Tandem mass tag proteomics revealed that numerous proteins were altered after nicotinamide adenine dinucleotide treatment in hypoxic-ischemic brain injury mice.Parallel reaction monitoring and western blotting confirmed changes in the expression levels of proteins including serine(or cysteine)peptidase inhibitor,clade A,member 3N,fibronectin 1,5'-nucleotidase,cytosolic IA,microtubule associated protein 2,and complexin 2.Proteomics analyses showed that nicotinamide adenine dinucleotide ameliorated hypoxic-ischemic injury through inflammation-related signaling pathways(e.g.,nuclear factor-kappa B,mitogen-activated protein kinase,and phosphatidylinositol 3 kinase/protein kinase B).These findings suggest that nicotinamide adenine dinucleotide treatment can improve neurobehavioral phenotypes in hypoxic-ischemic brain injury mice through inflammation-related pathways.展开更多
The mechanism of sphingosine-1-phosphate(S1P)-mediated phagocytosis remains unknown.Here,we found that S1P or FTY720(an analog of S1P)promoted microglial phagocytosis in stroke independent of S1PRs.First,we used compu...The mechanism of sphingosine-1-phosphate(S1P)-mediated phagocytosis remains unknown.Here,we found that S1P or FTY720(an analog of S1P)promoted microglial phagocytosis in stroke independent of S1PRs.First,we used computer simulation of molecular docking to predict that S1P might be a ligand for triggering receptor expressed on myeloid cells 2(TREM2).Next,microscale thermophoresis(MST),surface plasmon resonance(SPR)and liquid chromatography–tandem mass spectrometry(LC–MS/MS)were performed to reveal that S1P was a novel TREM2 ligand.Then,we confirmed the pro-phagocytosis of S1P targeting in Trem2-Dap12 transfected CHO cells and TREM2 knockdown microglia.Point mutation analysis showed that D104 was the critical binding residue.Trem2^(−/−)mice were used to demonstrate the role of S1P-induced phagocytosis targeting on TREM2 in protecting against ischemic brain injury.Finally,further studies revealed that apolipoprotein E(APOE)loaded with S1P was released by microglia and bound to apoptotic neurons via LDL receptor related protein 1B(LRP1B)and thereby induced microglia to phagocytose apoptotic neurons.Overall,the present work reveals for the first time that S1P acts as a novel endogenous ligand of TREM2 to effectively promote microglial phagocytosis.Our findings provide a new lead compound for developing immunomodulator targeting on TREM2.展开更多
本文是一篇近两个月的锂电池文献评述,以“lithium”和“battery*”为关键词检索了Web of Science从2023年12月1日至2024年1月31日上线的锂电池研究论文,共有6213篇,选择其中100篇加以评论。正极材料的研究集中于高镍三元、富锂正极材...本文是一篇近两个月的锂电池文献评述,以“lithium”和“battery*”为关键词检索了Web of Science从2023年12月1日至2024年1月31日上线的锂电池研究论文,共有6213篇,选择其中100篇加以评论。正极材料的研究集中于高镍三元、富锂正极材料的掺杂改性和表面包覆,以及其在长循环过程中的结构演变等。负极材料的研究重点包括硅基负极的界面调控和材料制备优化以缓冲体积变化、金属锂负极的界面构筑与调控。固态电解质的研究主要包括氯化物固态电解质、硫化物固态电解质和聚合物固态电解质的结构设计以及相关性能研究,电解液研究则主要包括不同电解质盐和溶剂对各类电池材料体系适配的研究,以及对新的功能性添加剂的探索。针对固态电池,正极材料的体相改性和表面包覆、复合正极制备与界面修饰、锂金属负极的界面构筑和三维结构设计有多篇文献报道。锂硫电池的研究重点是硫正极的结构设计、功能涂层和电解液的改进,固态锂硫电池也引起了广泛关注。电池工艺技术方面的研究包括干法等电极制备技术、黏结剂的研究。表征分析涵盖了正极材料的结构相变、锂沉积负极的界面演变等。理论模拟工作侧重于界面离子传输的研究,以及通过计算模拟来优化电极结构。展开更多
基金This study is mainly financially supported by the Beijing Natural Science Foundation(2182064)hosted by Prof.Rujie He.Prof.Rujie He also thanks the support from the National Natural Science Foundation of China(51772028)+2 种基金Prof.M i n Xia thanks the support from the Fundamental Research Funds for the Central Universities(3052017010)Prof.Xinxin Jin thanks the support from the National Natural Science Foundation of China(51602082)Dr.Keqiang Zhang thanks the support from the Graduate Technology Innovation Project of Beijing Institute of Technology(No.2019CX10020).
文摘Hydroxyapatite(HA)bioceramic scaffolds were fabricated by using digital light processing(DLP)based additive manufacturing.Key issues on the HA bioceramic scaffolds,including dispersion,DLP fabrication,sintering,mechanical properties,and biocompatibility were discussed in detail.Firstly,the ffects of dispersant dosage,solid loading,and sintering temperature were studied.The optimal dispersant dosage,solid loading,and sintering temperature were 2wt%,50vol%,and 1250℃,respectively.Then,the mechanical properties and biocompatibility of the HA bioceramic scaffolds were investigated.The DLP-prepared porous HA bioceramic scaffold was found to exhibit excellent mechanical properties and degradation behavior.From this study,DLP technique shows good potential for manufacturing HA bioceramic scaffolds.
基金This work was supported by the National Natural Science Foundation of China(Grant Nos.51972080 and 51772077)the Shenzhen Science and Technology Program(Grant No.KQTD2016112814303055)the science foundation of National Key Laboratory of Science and Technology on Advanced Composites in Special Environments.
文摘Mo_(2)Ga_(2)C is a new MAX phase with a stacking Ga-bilayer as well as possible unusual properties.To understand this unique MAX phase structure and promote possible future applications,the structure,chemical bonding,and mechanical and thermodynamic properties of Mo_(2)Ga_(2)C were investigated by first-principles.Using the“bond stiffness”model,the strongest covalent bonding(1162 GPa)was formed between Mo and C atoms in Mo_(2)Ga_(2)C,while the weakest Ga–Ga(389 GPa)bonding was formed between two Ga-atomic layers,different from other typical MAX phases.The ratio of the bond stiffness of the weakest bond to the strongest bond(0.33)was lower than 1/2,indicating the high damage tolerance and fracture toughness of Mo_(2)Ga_(2)C,which was confirmed by indentation without any cracks.The high-temperature heat capacity and thermal expansion of Mo_(2)Ga_(2)C were calculated in the framework of quasi-harmonic approximation from 0 to 1300 K.Because of the metal-like electronic structure,the electronic excitation contribution became more significant with increasing temperature above 300 K.
基金supported by the National Natural Science Foundation of China,Nos.81871024 (to HN),82301957 (to XW),82001382 (to LL),62127810 (to HN)the Natural Science Foundation of Jiangsu Province of China,No.SBK2020040785 (to LL)。
文摘Neonatal hypoxic-ischemic brain injury is the main cause of hypoxic-ischemic encephalopathy and cerebral palsy.Currently,there are few effective clinical treatments for neonatal hypoxic-ischemic brain injury.Here,we investigated the neuroprotective and molecular mechanisms of exogenous nicotinamide adenine dinucleotide,which can protect against hypoxic injury in adulthood,in a mouse model of neonatal hypoxic-ischemic brain injury.In this study,nicotinamide adenine dinucleotide(5 mg/kg)was intraperitoneally administered 30 minutes befo re surgery and every 24 hours thereafter.The results showed that nicotinamide adenine dinucleotide treatment improved body weight,brain structure,adenosine triphosphate levels,oxidative damage,neurobehavioral test outcomes,and seizure threshold in experimental mice.Tandem mass tag proteomics revealed that numerous proteins were altered after nicotinamide adenine dinucleotide treatment in hypoxic-ischemic brain injury mice.Parallel reaction monitoring and western blotting confirmed changes in the expression levels of proteins including serine(or cysteine)peptidase inhibitor,clade A,member 3N,fibronectin 1,5'-nucleotidase,cytosolic IA,microtubule associated protein 2,and complexin 2.Proteomics analyses showed that nicotinamide adenine dinucleotide ameliorated hypoxic-ischemic injury through inflammation-related signaling pathways(e.g.,nuclear factor-kappa B,mitogen-activated protein kinase,and phosphatidylinositol 3 kinase/protein kinase B).These findings suggest that nicotinamide adenine dinucleotide treatment can improve neurobehavioral phenotypes in hypoxic-ischemic brain injury mice through inflammation-related pathways.
基金supported by the National Natural Science Foundation of China (Nos.81973301, 82003732 and 81773701)the Medical Research Project of Jiangsu Commission of Health (No.ZDA2020006, China)+3 种基金the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (No.18KJA310004)the Major Project of Nanjing Medical University (No.NMUD2018008, China)the Postgraduate Research and Practice Innovation Program of Jiangsu Province (Nos.KYCX19_1121 and KYCX20_1417, China)Priority Academic Program Development of Jiangsu Higer Education Institutions (China)
文摘The mechanism of sphingosine-1-phosphate(S1P)-mediated phagocytosis remains unknown.Here,we found that S1P or FTY720(an analog of S1P)promoted microglial phagocytosis in stroke independent of S1PRs.First,we used computer simulation of molecular docking to predict that S1P might be a ligand for triggering receptor expressed on myeloid cells 2(TREM2).Next,microscale thermophoresis(MST),surface plasmon resonance(SPR)and liquid chromatography–tandem mass spectrometry(LC–MS/MS)were performed to reveal that S1P was a novel TREM2 ligand.Then,we confirmed the pro-phagocytosis of S1P targeting in Trem2-Dap12 transfected CHO cells and TREM2 knockdown microglia.Point mutation analysis showed that D104 was the critical binding residue.Trem2^(−/−)mice were used to demonstrate the role of S1P-induced phagocytosis targeting on TREM2 in protecting against ischemic brain injury.Finally,further studies revealed that apolipoprotein E(APOE)loaded with S1P was released by microglia and bound to apoptotic neurons via LDL receptor related protein 1B(LRP1B)and thereby induced microglia to phagocytose apoptotic neurons.Overall,the present work reveals for the first time that S1P acts as a novel endogenous ligand of TREM2 to effectively promote microglial phagocytosis.Our findings provide a new lead compound for developing immunomodulator targeting on TREM2.
文摘本文是一篇近两个月的锂电池文献评述,以“lithium”和“battery*”为关键词检索了Web of Science从2023年12月1日至2024年1月31日上线的锂电池研究论文,共有6213篇,选择其中100篇加以评论。正极材料的研究集中于高镍三元、富锂正极材料的掺杂改性和表面包覆,以及其在长循环过程中的结构演变等。负极材料的研究重点包括硅基负极的界面调控和材料制备优化以缓冲体积变化、金属锂负极的界面构筑与调控。固态电解质的研究主要包括氯化物固态电解质、硫化物固态电解质和聚合物固态电解质的结构设计以及相关性能研究,电解液研究则主要包括不同电解质盐和溶剂对各类电池材料体系适配的研究,以及对新的功能性添加剂的探索。针对固态电池,正极材料的体相改性和表面包覆、复合正极制备与界面修饰、锂金属负极的界面构筑和三维结构设计有多篇文献报道。锂硫电池的研究重点是硫正极的结构设计、功能涂层和电解液的改进,固态锂硫电池也引起了广泛关注。电池工艺技术方面的研究包括干法等电极制备技术、黏结剂的研究。表征分析涵盖了正极材料的结构相变、锂沉积负极的界面演变等。理论模拟工作侧重于界面离子传输的研究,以及通过计算模拟来优化电极结构。
