Chlorophyllid a binding protein （ chbp） was recently characterized by its ability to bind the prosthetic group of chlorophylls and little information is known regarding its expression. In the present study, we found...Chlorophyllid a binding protein （ chbp） was recently characterized by its ability to bind the prosthetic group of chlorophylls and little information is known regarding its expression. In the present study, we found that chpb was expressed highly and exclusively in the midgut of silkworm, Bombyx mori. The expression level of chbp was very high in the newly molted fifth instar larvae followed by gradual decline in the same instar. Our results demonstrated that CHBP was a secretory protein and located mainly in the apical of midgut epithelial cells. Real-time polymerase chain reaction analysis results showed that chpb highly expressed in the anterior midgut, threefold and sixfold higher compared with that of the middle midgut and posterior midgut, respectively, and chpb expression declined in darkness. In addition, the expression of chbp was affected by high-dose virus or bacterium infection.展开更多
In this study,a new type of water-soluble polysaccharide was extracted and purified from mussels,and its structure and cellular antioxidant levels were studied.Mussel polysaccharide(MP 4.25×10^(3) Da)is composed ...In this study,a new type of water-soluble polysaccharide was extracted and purified from mussels,and its structure and cellular antioxidant levels were studied.Mussel polysaccharide(MP 4.25×10^(3) Da)is composed mainly of glucose,with a small amount of galactosamine hydrochloride,glucosamine hydrochloride,and galactose.MP shows characteristic polysaccharide bands in Fourier transform NMR and infrared spectroscopy.The main chain connection mode of MP is the glycosidic bond of→4)-α-D-Glcp-(1→,and the end groupα-D-Glcp-(1→andα-D-Glcp-(1→6)-α-D-Glcp-(1→pass→4,6)-α-D-Glcp-(1→.The O-6 bond is connected to the main chain.Moreover,MP could protect RAW264.7 cells against H_(2)O_(2)-induced oxidative stress by increasing catalase(CAT),glutathione peroxidase(GSH-Px),and superoxide dismutase(SOD)activities and reducing the content of malondialdehyde(MDA).This is expected to make MP an important member of the antioxidant family.展开更多
In the late phase of Bombyx mori nucleopolyhedrovirus(BmNPV)infection,a large amount of polyhedra appear in the infected cell nucleolus,these polyhedra being dense protein crystals protecting the incorporated virions ...In the late phase of Bombyx mori nucleopolyhedrovirus(BmNPV)infection,a large amount of polyhedra appear in the infected cell nucleolus,these polyhedra being dense protein crystals protecting the incorporated virions from the harsh environment.To investigate whether the foreign protein could be immobilized into the polyhedra of BmNPV,two recombinant baculoviruses were generated by a novel BmNPV polyhedrin-plus(polh +)Bac-to-Bac system,designated as vBmBac(polh +)-enhanced green fluorescent protein(EGFP)and vBmBac(polh +)-LacZ,which can express the polyhedrin and foreign protein simultaneously.Light microscopy analysis showed that all viruses produced polyhedra of normal appearance.Green fluorescence can be apparently detected on the surface of the vBmBac(polh +)-EGFP polyhedra,but not the BmNPV polyhedra.Fluorescence analysis and anti-desiccation testing confirmed that EGFP was embedded in the polyhedra.As expected,the vBmBac(polh +)-LacZ polyhedra contained an amount of LacZ and had a higherβ-galactosidase activity.Sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE)and Western blotting were also performed to verify if the foreign proteins were immobilized into polyhedra.This study provides a new inspiration for efficient preservation of useful proteins and development of new pesticides with toxic proteins.展开更多
文摘Chlorophyllid a binding protein （ chbp） was recently characterized by its ability to bind the prosthetic group of chlorophylls and little information is known regarding its expression. In the present study, we found that chpb was expressed highly and exclusively in the midgut of silkworm, Bombyx mori. The expression level of chbp was very high in the newly molted fifth instar larvae followed by gradual decline in the same instar. Our results demonstrated that CHBP was a secretory protein and located mainly in the apical of midgut epithelial cells. Real-time polymerase chain reaction analysis results showed that chpb highly expressed in the anterior midgut, threefold and sixfold higher compared with that of the middle midgut and posterior midgut, respectively, and chpb expression declined in darkness. In addition, the expression of chbp was affected by high-dose virus or bacterium infection.
基金supported by grants from National Key Research and Development Program of China(2020YFD0900902)General scientific research project of Zhejiang Provincial Department of Education(Y202043175).
文摘In this study,a new type of water-soluble polysaccharide was extracted and purified from mussels,and its structure and cellular antioxidant levels were studied.Mussel polysaccharide(MP 4.25×10^(3) Da)is composed mainly of glucose,with a small amount of galactosamine hydrochloride,glucosamine hydrochloride,and galactose.MP shows characteristic polysaccharide bands in Fourier transform NMR and infrared spectroscopy.The main chain connection mode of MP is the glycosidic bond of→4)-α-D-Glcp-(1→,and the end groupα-D-Glcp-(1→andα-D-Glcp-(1→6)-α-D-Glcp-(1→pass→4,6)-α-D-Glcp-(1→.The O-6 bond is connected to the main chain.Moreover,MP could protect RAW264.7 cells against H_(2)O_(2)-induced oxidative stress by increasing catalase(CAT),glutathione peroxidase(GSH-Px),and superoxide dismutase(SOD)activities and reducing the content of malondialdehyde(MDA).This is expected to make MP an important member of the antioxidant family.
基金supported by the National Basic Research Program(973)of China(No.2012CB114600)the Zhejiang Provincial Natural Science Foundation(No.Y3110058)the Public Agricultural Program of Zhejiang Province(No.2012C32G2010076),China
文摘In the late phase of Bombyx mori nucleopolyhedrovirus(BmNPV)infection,a large amount of polyhedra appear in the infected cell nucleolus,these polyhedra being dense protein crystals protecting the incorporated virions from the harsh environment.To investigate whether the foreign protein could be immobilized into the polyhedra of BmNPV,two recombinant baculoviruses were generated by a novel BmNPV polyhedrin-plus(polh +)Bac-to-Bac system,designated as vBmBac(polh +)-enhanced green fluorescent protein(EGFP)and vBmBac(polh +)-LacZ,which can express the polyhedrin and foreign protein simultaneously.Light microscopy analysis showed that all viruses produced polyhedra of normal appearance.Green fluorescence can be apparently detected on the surface of the vBmBac(polh +)-EGFP polyhedra,but not the BmNPV polyhedra.Fluorescence analysis and anti-desiccation testing confirmed that EGFP was embedded in the polyhedra.As expected,the vBmBac(polh +)-LacZ polyhedra contained an amount of LacZ and had a higherβ-galactosidase activity.Sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE)and Western blotting were also performed to verify if the foreign proteins were immobilized into polyhedra.This study provides a new inspiration for efficient preservation of useful proteins and development of new pesticides with toxic proteins.
