目的研究妊娠晚期孕妇生殖道定植及婴儿侵袭性感染的B族链球菌(group B Streptococcus,GBS)血清型、基因型及耐药性,为临床防治GBS感染提供参考。方法收集2015年6月-2018年6月在厦门市妇幼保健院建卡并分娩的孕晚期生殖道GBS定植菌株299...目的研究妊娠晚期孕妇生殖道定植及婴儿侵袭性感染的B族链球菌(group B Streptococcus,GBS)血清型、基因型及耐药性,为临床防治GBS感染提供参考。方法收集2015年6月-2018年6月在厦门市妇幼保健院建卡并分娩的孕晚期生殖道GBS定植菌株299株,同期厦门市妇幼保健院0~89 d婴儿侵袭性感染GBS菌株83株。采用乳胶凝集试验检测GBS菌株的血清型,管家基因测序确定菌株的序列分型(sequencing typing,ST),纸片扩散法检测细菌对常用抗菌药物的敏感性。结果299例孕妇定植GBS共发现8个血清型,按占比率高低前五位为:Ⅲ型54.2%、Ⅰb型17.7%、Ⅰa型10.0%、Ⅴ型9.7%、Ⅱ型8.0%;83例患儿侵袭性感染GBS菌株共发现5个血清型,按占比率高低依次为:Ⅲ型59.0%、Ⅰa型15.7%、Ⅰb型14.5%、Ⅱ型4.8%、Ⅴ型3.6%,另有两例为不定型(non-typeable,NT)。孕妇定植与患儿侵袭性感染菌株的血清型分布比较无显著差异。299例孕妇定植GBS菌株中有246株进行基因分型,共发现40个基因型,按占比率前五位为:ST19占21.1%(52/246)、ST862占11.4%(28/246)、ST12占8.5%(21/246)、ST10占7.7%(19/246)、ST17占6.1%(15/246);83例婴儿感染GBS菌株共发现19个基因型,按占比率前五位为:ST17占41.0%、ST23占12.0%、ST19占10.8%、ST12占7.2%、ST10占4.8%,两组基因型分布差异有统计学意义。GBS菌株对青霉素、氨苄青霉素、头孢唑林、美罗培南、万古霉素的耐药率均为0%,对红霉素、克林霉素、阿奇霉素的耐药率在63.2%~91.6%,且婴儿感染GBS组对红霉素及克林霉素的耐药率均高于孕妇定植GBS组,两组耐药率比较差异有统计学意义。结论本地区孕妇定植GBS及婴儿感染GBS血清型分布前五位均为:Ⅲ型、Ⅰb型、Ⅰa型、Ⅴ型及Ⅱ型;ST19是孕妇定植GBS的主要基因型,而ST17是婴儿侵袭性GBS感染的最主要基因型;GBS对红霉素、克林霉素及阿奇霉素的耐药率极高。展开更多
Chinese Expeditionary Force(CEF)was one of the two Chinese military excursions outside of sovereign Chinese territory sent to repel Japanese forces occupying Burma during World War II.By 1941,the World War II entered ...Chinese Expeditionary Force(CEF)was one of the two Chinese military excursions outside of sovereign Chinese territory sent to repel Japanese forces occupying Burma during World War II.By 1941,the World War II entered its darkest days.China had been embroiled in a desperate struggle to resist the Japanese invasion.Japanese troops aiming at cutting the supply routes to China had left only one line of communication open for China to the West—the Yunnan-Burma Road in the southwest[1].To展开更多
Background： Trichophyton rubrum represents the most common infectious fungus responsible for dermatophytosis in human, but the mechanism involved is still not completely understood. An appropriate model constructed t...Background： Trichophyton rubrum represents the most common infectious fungus responsible for dermatophytosis in human, but the mechanism involved is still not completely understood. An appropriate model constructed to simulate host infection is the prerequisite to study the pathogenesis of dernlatophytosis caused by T.. rubrum. In this study, we intended to develop a new T. rubrum infection model in vitro, using the three-dimensional reconstructed epidermis - EpiSkin, and to pave the way for further investigation of the mechanisms involved in T. rubrum infection. Methods： The reconstructed human epidermis （RHE） was infected by inoculating low-dose （400 conidia） and high-dose （4000 conidia） T. rubrum conidia to optimize the infection dose. During the various periods after infection, the samples were processed for pathological examination and scanning electron microscopy （SEM） observation. Results： The histological analysis of RHE revealed a fully differentiated epidermis with a functional stratum corneum, which was analogous to the normal human epidermis. The results of hematoxylin and eosin staining and the periodic acid-Schiff staining showed that the infection dose of 400 conidia was in accord with the pathological characteristics of host dermatophytosis caused by T. rubrum. SEM observations further exhibited the process of 77 ruhrum infection in an intuitionistic way, Conclusions： We established the T. rubrum infection model on RHE in vitro successfully. It is a promising model fbr further investigation of the mechanisms involved in T. rubrum infection.展开更多
The recently emerged double-cable conjugated polymers have come into focus due to their significantly improved power conversion efficiencies (PCEs) in single-component organic solar cells (SCOSCs). In this work, the e...The recently emerged double-cable conjugated polymers have come into focus due to their significantly improved power conversion efficiencies (PCEs) in single-component organic solar cells (SCOSCs). In this work, the effect of chlorination in double-cable conjugated polymers with linear benzodithiophene backbone and pendant perylene bisimide on the photovoltaic performance in SCOSCs has been studied. After introducing chlorine atoms into conjugated side chains, the highest occupied molecular orbital level of the conjugated polymers is down-shifted, thus resulting in a higher open-circuit voltage. As a result, the chlorinated double-cable conjugated polymer exhibits improved photovoltaic performance from 3.46% to 3.57%.展开更多
目的探讨维生素E琥珀酸酯(vitamin E succinate,VES)对人胃癌SGC-7901细胞线粒体凋亡通路中第二种线粒体来源半胱氨酸蛋白酶激活剂(secondmitochondria-derivedactivatorofcaspases,Smac)、X连锁凋亡抑制蛋白(X-linked inhibitor of apo...目的探讨维生素E琥珀酸酯(vitamin E succinate,VES)对人胃癌SGC-7901细胞线粒体凋亡通路中第二种线粒体来源半胱氨酸蛋白酶激活剂(secondmitochondria-derivedactivatorofcaspases,Smac)、X连锁凋亡抑制蛋白(X-linked inhibitor of apoptosis protein,XIAP)基因和蛋白表达的影响及其诱导凋亡机制。方法不同浓度VES(5、10、15、20μg/ml)处理人胃癌细胞24h,0.01%的无水乙醇设为对照组。CCK-8检测细胞增殖活性;实时荧光定量PCR检测线粒体相关凋亡分子Smac、XIAP mRNA表达变化;Western blot检测线粒体相关凋亡分子Smac、XIAP蛋白表达水平的差异;Annexin-V/PI双染流式细胞术检测各组细胞凋亡率。结果与对照组相比,不同浓度VES(5、10、15、20μg/ml)作用胃癌细胞后的细胞增殖率分别为94.67%±0.33%、85.28%±0.82%、67.63%±1.3%、52.11%±2.3%,VES抑制细胞增殖的效果随VES浓度升高而增强(P<0.05);Smac mRNA随着VES剂量的增加而增加(P<0.05),在15μg/ml达到峰值(P<0.05),XIAPmRNA随着VES剂量的增加而下降(P<0.05);XIAP蛋白在5、10μg/mlVES处理组表达量差异无统计学意义,在15、20μg/ml VES处理组,表达量差异有统计学意义(P<0.05),蛋白表达量随着浓度升高(P<0.05),Smac蛋白蛋白表达量随着VES剂量的增加明显升高(P<0.05);不同浓度VES(5、10、15、20μg/ml)处理组凋亡率依次为2.06%±0.21%、8.17%±0.56%、13.30%±0.33%、19.82%±0.95%、34.45%±1.78%,VES诱导细胞凋亡的效果随VES作用浓度升高而升高(P<0.05)。结论VES可以抑制胃癌细胞增殖、诱导细胞凋亡,在转录和翻译水平上上调Smac的表达、抑制XIAP的表达。展开更多
Background： Trichophyton rubrum is superficial fungi characteristically confined to dead keratinized tissues. These observations suggest that the soluble components released by the fungus could influence the host imm...Background： Trichophyton rubrum is superficial fungi characteristically confined to dead keratinized tissues. These observations suggest that the soluble components released by the fungus could influence the host immune response in a cell in contact-free manner. Therefore, this research aimed to analyze whether the culture supernatant derived from T. rubrum grown in the nail medium could elicit the immune response of keratinocyte effectively. Methods： The culture supernatants of two strains （T1a, TXHB） were compared for the β-glucan concentrations and their capacity to impact the innate immunity of keratinocytes. The β-glucan concentrations in the supernatants were determined with the fungal G-test kit and protein concentrations with bicinchoninic acid protein quantitative method, then HaCaT was stimulated with different concentrations of culture supernatants by adopting morphological method to select a suitable dosage. Expressions of host defense genes were assessed by quantitative polymerase chain reaction after the HaCaT was stimulated with the culture supernatants. Data were analyzed with one-way analysis of variance, followed by the least significant difference test. Results： The T. rubrum strains （T1a and TXHB） released β-glucan of 87.530 ± 37.581 pg/ml and 15.747 ± 6.453 pg/ml, respectively into the media. The messenger RNA （mRNA） expressions of toll-like receptor-2 （TLR2）, TLR4, and CARD9 were moderately up-regulated in HaCaT within 6-h applications of both supernatants. HaCaT cells were more responsive to Tla than TXHB. The slight increase of dendritic cells-specific intercellular adhesion molecule 3-grabbing nonintegrin expression was faster and stronger, induced by T1a supematant than TXHB. The moderate decreases of RNase 7, the slight up-regulations of Dectin-1 and interleukin-8 at the mRNA level were detected only in response to T la rather than TXHB After a long-time contact, all the elevated defense genes decreased alter 24 h. Conclusion： The culture supernatant of T. r展开更多
In the past few decades,tremendous advances have been made in terms of advaneed non-small cell lung cancer(NSCLC).The identification of oncogenic drivers and the development of targeted therapies led to the developmen...In the past few decades,tremendous advances have been made in terms of advaneed non-small cell lung cancer(NSCLC).The identification of oncogenic drivers and the development of targeted therapies led to the development of"pre&sion"medicine.On the other hand,more attention has been paid to cancer immune escape and a new class of immunomodulatory agents has been developed.It is very attractive to explore the combination strategies of the two approaches,which may also be meaningful from the perspective of immunology.展开更多
Aim The purpose of this study is to compare the effects of the two clutches on recording the condylar movement. Methodology Ten subjects （6 women,4 men; mean age 25.4 years） participated in the study. The mandibular...Aim The purpose of this study is to compare the effects of the two clutches on recording the condylar movement. Methodology Ten subjects （6 women,4 men; mean age 25.4 years） participated in the study. The mandibular move-ment,sagittal condylar inclination angle,and transversal condylar inclination angle of each subject were recorded with the CADIAX？ using the two clutches,respectively. The characteristics of the tracings of the protrusion,opening,and mediotrusion were analyzed with the t-test statistics at α = 0.05 level. The Kappa values were calculated for an assessment of the congruence of the tracings. Results The results showed that the contour,direction,and dimension of the tracings in the two clutches were approxi-mately same,but the tracings determined by the functional occlusal clutch were more regular and congruent. In the group segment recorded with the tray clutch,opening/ closing paths of one subject showed crossed and time curves of three subjects appeared peak-like changes of velocity,but none were statistically different （P0.05）. Conclusion The research suggests that the functional occlusal clutch should be preferred in the evaluation of the mandibular function,as the tracings with the tray clutch are more likely to produce false positive results.展开更多
文摘目的研究妊娠晚期孕妇生殖道定植及婴儿侵袭性感染的B族链球菌(group B Streptococcus,GBS)血清型、基因型及耐药性,为临床防治GBS感染提供参考。方法收集2015年6月-2018年6月在厦门市妇幼保健院建卡并分娩的孕晚期生殖道GBS定植菌株299株,同期厦门市妇幼保健院0~89 d婴儿侵袭性感染GBS菌株83株。采用乳胶凝集试验检测GBS菌株的血清型,管家基因测序确定菌株的序列分型(sequencing typing,ST),纸片扩散法检测细菌对常用抗菌药物的敏感性。结果299例孕妇定植GBS共发现8个血清型,按占比率高低前五位为:Ⅲ型54.2%、Ⅰb型17.7%、Ⅰa型10.0%、Ⅴ型9.7%、Ⅱ型8.0%;83例患儿侵袭性感染GBS菌株共发现5个血清型,按占比率高低依次为:Ⅲ型59.0%、Ⅰa型15.7%、Ⅰb型14.5%、Ⅱ型4.8%、Ⅴ型3.6%,另有两例为不定型(non-typeable,NT)。孕妇定植与患儿侵袭性感染菌株的血清型分布比较无显著差异。299例孕妇定植GBS菌株中有246株进行基因分型,共发现40个基因型,按占比率前五位为:ST19占21.1%(52/246)、ST862占11.4%(28/246)、ST12占8.5%(21/246)、ST10占7.7%(19/246)、ST17占6.1%(15/246);83例婴儿感染GBS菌株共发现19个基因型,按占比率前五位为:ST17占41.0%、ST23占12.0%、ST19占10.8%、ST12占7.2%、ST10占4.8%,两组基因型分布差异有统计学意义。GBS菌株对青霉素、氨苄青霉素、头孢唑林、美罗培南、万古霉素的耐药率均为0%,对红霉素、克林霉素、阿奇霉素的耐药率在63.2%~91.6%,且婴儿感染GBS组对红霉素及克林霉素的耐药率均高于孕妇定植GBS组,两组耐药率比较差异有统计学意义。结论本地区孕妇定植GBS及婴儿感染GBS血清型分布前五位均为:Ⅲ型、Ⅰb型、Ⅰa型、Ⅴ型及Ⅱ型;ST19是孕妇定植GBS的主要基因型,而ST17是婴儿侵袭性GBS感染的最主要基因型;GBS对红霉素、克林霉素及阿奇霉素的耐药率极高。
基金supported by NSFC for Excellent Young Scholar (31222030)MOE Scientific Research Project (113022A)Shanghai Shuguang Project (14SG05)
文摘Chinese Expeditionary Force(CEF)was one of the two Chinese military excursions outside of sovereign Chinese territory sent to repel Japanese forces occupying Burma during World War II.By 1941,the World War II entered its darkest days.China had been embroiled in a desperate struggle to resist the Japanese invasion.Japanese troops aiming at cutting the supply routes to China had left only one line of communication open for China to the West—the Yunnan-Burma Road in the southwest[1].To
基金This study was supported by the grants from Guangdong Natural Science Foundation，the Special Fund for Young Scientists of Third Affiliated Hospital of Sun Yat-sen University
文摘Background： Trichophyton rubrum represents the most common infectious fungus responsible for dermatophytosis in human, but the mechanism involved is still not completely understood. An appropriate model constructed to simulate host infection is the prerequisite to study the pathogenesis of dernlatophytosis caused by T.. rubrum. In this study, we intended to develop a new T. rubrum infection model in vitro, using the three-dimensional reconstructed epidermis - EpiSkin, and to pave the way for further investigation of the mechanisms involved in T. rubrum infection. Methods： The reconstructed human epidermis （RHE） was infected by inoculating low-dose （400 conidia） and high-dose （4000 conidia） T. rubrum conidia to optimize the infection dose. During the various periods after infection, the samples were processed for pathological examination and scanning electron microscopy （SEM） observation. Results： The histological analysis of RHE revealed a fully differentiated epidermis with a functional stratum corneum, which was analogous to the normal human epidermis. The results of hematoxylin and eosin staining and the periodic acid-Schiff staining showed that the infection dose of 400 conidia was in accord with the pathological characteristics of host dermatophytosis caused by T. rubrum. SEM observations further exhibited the process of 77 ruhrum infection in an intuitionistic way, Conclusions： We established the T. rubrum infection model on RHE in vitro successfully. It is a promising model fbr further investigation of the mechanisms involved in T. rubrum infection.
基金the National Natural Science Foundation of China(Nos.51973169,51703172 and 52073016)the Open Project Program of Wuhan National Laboratory for Optoelectronics(No.2020WNLOKF015)the science foundation of Wuhan Institute of Technology(No.K202025).
文摘The recently emerged double-cable conjugated polymers have come into focus due to their significantly improved power conversion efficiencies (PCEs) in single-component organic solar cells (SCOSCs). In this work, the effect of chlorination in double-cable conjugated polymers with linear benzodithiophene backbone and pendant perylene bisimide on the photovoltaic performance in SCOSCs has been studied. After introducing chlorine atoms into conjugated side chains, the highest occupied molecular orbital level of the conjugated polymers is down-shifted, thus resulting in a higher open-circuit voltage. As a result, the chlorinated double-cable conjugated polymer exhibits improved photovoltaic performance from 3.46% to 3.57%.
文摘Background： Trichophyton rubrum is superficial fungi characteristically confined to dead keratinized tissues. These observations suggest that the soluble components released by the fungus could influence the host immune response in a cell in contact-free manner. Therefore, this research aimed to analyze whether the culture supernatant derived from T. rubrum grown in the nail medium could elicit the immune response of keratinocyte effectively. Methods： The culture supernatants of two strains （T1a, TXHB） were compared for the β-glucan concentrations and their capacity to impact the innate immunity of keratinocytes. The β-glucan concentrations in the supernatants were determined with the fungal G-test kit and protein concentrations with bicinchoninic acid protein quantitative method, then HaCaT was stimulated with different concentrations of culture supernatants by adopting morphological method to select a suitable dosage. Expressions of host defense genes were assessed by quantitative polymerase chain reaction after the HaCaT was stimulated with the culture supernatants. Data were analyzed with one-way analysis of variance, followed by the least significant difference test. Results： The T. rubrum strains （T1a and TXHB） released β-glucan of 87.530 ± 37.581 pg/ml and 15.747 ± 6.453 pg/ml, respectively into the media. The messenger RNA （mRNA） expressions of toll-like receptor-2 （TLR2）, TLR4, and CARD9 were moderately up-regulated in HaCaT within 6-h applications of both supernatants. HaCaT cells were more responsive to Tla than TXHB. The slight increase of dendritic cells-specific intercellular adhesion molecule 3-grabbing nonintegrin expression was faster and stronger, induced by T1a supematant than TXHB. The moderate decreases of RNase 7, the slight up-regulations of Dectin-1 and interleukin-8 at the mRNA level were detected only in response to T la rather than TXHB After a long-time contact, all the elevated defense genes decreased alter 24 h. Conclusion： The culture supernatant of T. r
基金the grants from the National Natural Science Foundation of China(No.81672896)the National Key Research and Development Program:The key technology of palliative care and nursing for cancer patients(No.ZDZX2017ZL-01)High Level Innovation Team of Nanjing Medical University(No.JX102GSP201727).
文摘In the past few decades,tremendous advances have been made in terms of advaneed non-small cell lung cancer(NSCLC).The identification of oncogenic drivers and the development of targeted therapies led to the development of"pre&sion"medicine.On the other hand,more attention has been paid to cancer immune escape and a new class of immunomodulatory agents has been developed.It is very attractive to explore the combination strategies of the two approaches,which may also be meaningful from the perspective of immunology.
文摘Aim The purpose of this study is to compare the effects of the two clutches on recording the condylar movement. Methodology Ten subjects （6 women,4 men; mean age 25.4 years） participated in the study. The mandibular move-ment,sagittal condylar inclination angle,and transversal condylar inclination angle of each subject were recorded with the CADIAX？ using the two clutches,respectively. The characteristics of the tracings of the protrusion,opening,and mediotrusion were analyzed with the t-test statistics at α = 0.05 level. The Kappa values were calculated for an assessment of the congruence of the tracings. Results The results showed that the contour,direction,and dimension of the tracings in the two clutches were approxi-mately same,but the tracings determined by the functional occlusal clutch were more regular and congruent. In the group segment recorded with the tray clutch,opening/ closing paths of one subject showed crossed and time curves of three subjects appeared peak-like changes of velocity,but none were statistically different （P0.05）. Conclusion The research suggests that the functional occlusal clutch should be preferred in the evaluation of the mandibular function,as the tracings with the tray clutch are more likely to produce false positive results.
