Purpose: Interferon-γ (INF-γ) is a cytokine that participates in the immune reaction of the body. Its level of secretion can reflect the immune response condition after the body is infected by pathogens, which is a ...Purpose: Interferon-γ (INF-γ) is a cytokine that participates in the immune reaction of the body. Its level of secretion can reflect the immune response condition after the body is infected by pathogens, which is a significant indication of clinically-related diseases. Therefore, it is of great significance in application to develop a fluorescence biosensor to inspect INF-γ with rapidness, high sensitivity and high practicability. Method: The fluorescence sensor is made on the basis of the two-dimensional nano-material namely Carbon Nitride Nanosheet (CNNS) and the Aptamer probe to identify INF-γ (Apt®INF-γ). CNNS can quickly quench the Cy5 fluorescent dye modified on the Apt®INF-γ probe due to the Photoinduced Electron Transfer (PET), but when the INF-γ exists, Apt®INF-γ specifically identifies and combines it. The complex of Apt®INF-γ and INF-γ is away from CNNS, which can effectively block the fluorescent signal of Apt?INF-γ being quenched by CNNS. Result: The sensitive detection of IFN-γ protein can be achieved through the application of CNNS/Apt®INF-γ fluorescence sensing platform. In this method, the intensity of the fluorescent signal is positively correlated with the concentration of IFN-γ, of which the liner response range is 0.5 - 100 ng/mL and the limit of detection is 0.303 ng/mL. In addition, this fluorescence sensing platform has the advantages of high specificity, simple operation and low costs. It can inspect the content of IFN-γ in clinical serum samples without interference. The actual recovery rate of serum samples is 97.11% - 106.96%. Conclusion: Therefore, the CNNS/Apt®INF-γ sensing platform is expected to be implemented in the actual clinical detection, also conducive to developing a universal fluorescence biosensor to inspect other target materials.展开更多
Purpose: Aflatoxin B<sub>1</sub> is the most common mycotoxin in cereal crops;it is of stronger toxicity and has a carcinogenic effect. In recent years, a series of fluorescence sensors constructed on the ...Purpose: Aflatoxin B<sub>1</sub> is the most common mycotoxin in cereal crops;it is of stronger toxicity and has a carcinogenic effect. In recent years, a series of fluorescence sensors constructed on the basis of MoS<sub>2</sub>NS fluorescence quenching property have become a research hotspot. Therefore, we can construct a fast and simple analysis method with high specificity to detect AFB<sub>1</sub> by utilizing MoS<sub>2</sub>NS, which can be effectively applied to food safety monitoring and clinical diagnosis. Method: In the current research, a fluorescence biosensor is developed on the basis of a new type of two-dimensional nano-material namely MoS<sub>2</sub>NS applied for the detection of AFB<sub>1</sub>. The fluorescence of Apt@AFB<sub>1</sub> can be quickly quenched by MoS<sub>2</sub>NS through the fluorescence resonance energy transfer (FRET). When the target molecule AFB<sub>1</sub> exists, after the specificity binding between AFB<sub>1</sub> and aptamer, the Apt@AFB<sub>1</sub> loses its single stranded structure and is away from MoS<sub>2</sub>NS, and the fluorescence of Apt®AFB<sub>1</sub> cannot be quenched effectively. Such sensing signals can be used to achieve the sensitive detection of AFB<sub>1</sub>. Result: With this new method, under the optimized conditions, the AFB<sub>1</sub> is analyzed in the MoS<sub>2</sub>NS/Apt®AFB<sub>1</sub> sensing platform. Within the dynamic range of 0.2 - 25 ng/mL, the sensing platform expresses a good linear response to the level of AFB<sub>1</sub> with the R<sup>2</sup> = 0.9964 and LOD as 90 pg/mL. This method is applied to detect the actual serum samples and soybean milk with the recovery rate of 93.10% - 107.23% and 95.15% - 102.60% separately, and it can be used in the quantitative detection under the interference of other mycotoxins in a relatively accurate way. Conclusion: It is proved that this new detection method can be used as a potential biosensor platform for the detection of AFB<sub>1</sub>. This detection method features several advantages 展开更多
文摘Purpose: Interferon-γ (INF-γ) is a cytokine that participates in the immune reaction of the body. Its level of secretion can reflect the immune response condition after the body is infected by pathogens, which is a significant indication of clinically-related diseases. Therefore, it is of great significance in application to develop a fluorescence biosensor to inspect INF-γ with rapidness, high sensitivity and high practicability. Method: The fluorescence sensor is made on the basis of the two-dimensional nano-material namely Carbon Nitride Nanosheet (CNNS) and the Aptamer probe to identify INF-γ (Apt®INF-γ). CNNS can quickly quench the Cy5 fluorescent dye modified on the Apt®INF-γ probe due to the Photoinduced Electron Transfer (PET), but when the INF-γ exists, Apt®INF-γ specifically identifies and combines it. The complex of Apt®INF-γ and INF-γ is away from CNNS, which can effectively block the fluorescent signal of Apt?INF-γ being quenched by CNNS. Result: The sensitive detection of IFN-γ protein can be achieved through the application of CNNS/Apt®INF-γ fluorescence sensing platform. In this method, the intensity of the fluorescent signal is positively correlated with the concentration of IFN-γ, of which the liner response range is 0.5 - 100 ng/mL and the limit of detection is 0.303 ng/mL. In addition, this fluorescence sensing platform has the advantages of high specificity, simple operation and low costs. It can inspect the content of IFN-γ in clinical serum samples without interference. The actual recovery rate of serum samples is 97.11% - 106.96%. Conclusion: Therefore, the CNNS/Apt®INF-γ sensing platform is expected to be implemented in the actual clinical detection, also conducive to developing a universal fluorescence biosensor to inspect other target materials.
文摘Purpose: Aflatoxin B<sub>1</sub> is the most common mycotoxin in cereal crops;it is of stronger toxicity and has a carcinogenic effect. In recent years, a series of fluorescence sensors constructed on the basis of MoS<sub>2</sub>NS fluorescence quenching property have become a research hotspot. Therefore, we can construct a fast and simple analysis method with high specificity to detect AFB<sub>1</sub> by utilizing MoS<sub>2</sub>NS, which can be effectively applied to food safety monitoring and clinical diagnosis. Method: In the current research, a fluorescence biosensor is developed on the basis of a new type of two-dimensional nano-material namely MoS<sub>2</sub>NS applied for the detection of AFB<sub>1</sub>. The fluorescence of Apt@AFB<sub>1</sub> can be quickly quenched by MoS<sub>2</sub>NS through the fluorescence resonance energy transfer (FRET). When the target molecule AFB<sub>1</sub> exists, after the specificity binding between AFB<sub>1</sub> and aptamer, the Apt@AFB<sub>1</sub> loses its single stranded structure and is away from MoS<sub>2</sub>NS, and the fluorescence of Apt®AFB<sub>1</sub> cannot be quenched effectively. Such sensing signals can be used to achieve the sensitive detection of AFB<sub>1</sub>. Result: With this new method, under the optimized conditions, the AFB<sub>1</sub> is analyzed in the MoS<sub>2</sub>NS/Apt®AFB<sub>1</sub> sensing platform. Within the dynamic range of 0.2 - 25 ng/mL, the sensing platform expresses a good linear response to the level of AFB<sub>1</sub> with the R<sup>2</sup> = 0.9964 and LOD as 90 pg/mL. This method is applied to detect the actual serum samples and soybean milk with the recovery rate of 93.10% - 107.23% and 95.15% - 102.60% separately, and it can be used in the quantitative detection under the interference of other mycotoxins in a relatively accurate way. Conclusion: It is proved that this new detection method can be used as a potential biosensor platform for the detection of AFB<sub>1</sub>. This detection method features several advantages
