Silicosis is a leading cause of occupational disease-related morbidity and mortality worldwide,but the molecular basis underlying its development remains unclear.An accumulating body of evidence supports gasdermin D(G...Silicosis is a leading cause of occupational disease-related morbidity and mortality worldwide,but the molecular basis underlying its development remains unclear.An accumulating body of evidence supports gasdermin D(GSDMD)-mediated pyroptosis as a key component in the development of various pulmonary diseases.However,there is little experimental evidence connecting silicosis and GSDMD-driven pyroptosis.In this work,we investigated the role of GSDMD-mediated pyroptosis in silicosis.Single-cell RNA sequencing of healthy and silicosis human and murine lung tissues indicated that GSDMD-induced pyroptosis in macrophages was relevant to silicosis progression.Through microscopy we then observed morphological alterations of pyroptosis in macrophages treated with silica.Measurement of interleukin-1βrelease,lactic dehydrogenase activity,and real-time propidium iodide staining further revealed that silica induced pyroptosis of macrophages.Additionally,we verified that both canonical(caspase-1-mediated)and non-canonical(caspase-4/5/11-mediated)signaling pathways mediated silica-induced pyroptosis activation,in vivo and in vitro.Notably,Gsdmd knockout mice exhibited dramatically alleviated silicosis phenotypes,which highlighted the pivotal role of pyroptosis in this disease.Taken together,our results demonstrated that macrophages underwent GSDMD-dependent pyroptosis in silicosis and inhibition of this process could serve as a viable clinical strategy for mitigating silicosis.展开更多
The past decade has witnessed significant efforts in addressing the global metallic corrosion challenge,with a focus on avoiding or mitigating huge economic losses incurred by corrosion and on the development of prote...The past decade has witnessed significant efforts in addressing the global metallic corrosion challenge,with a focus on avoiding or mitigating huge economic losses incurred by corrosion and on the development of protective coatings on metals.Herein,a synergistic anticorrosion coating with both superhydrophobicity and self-healing properties was reported,through a facile replica molding method by mixing the polyvinylidene fluoride(PVDF)matrix with nano-sized SiO2 particles and 2-mercaptobenzothiazole(MBT)loaded halloysites(HNTs).The surface exhibits robust self-cleaning behavior under harsh conditions and high liquid repellence to withstand the osmosis of corrosive ions.The self-healing performance of the coating,due to the introduction of MBT-loaded HNTs,enhances the anticorrosion capability,which is still valid once the protective layer is damaged.Potentiodynamic polarization(PDP)and Electrochemical Impedance Spectroscopy(EIS)measurements demonstrate that the synergetic effects in anticorrosion performances significantly enhance the long-term corrosion protection of metals.Hence,this type of dual-action coating may find unique applications in metal corrosion resistance where both super-repellency and self-healing properties are desired.展开更多
为揭示大叶茶(Camellia sinensis var.assamica,CSA)研究的热点及前沿,研究采用文献计量学的方法,利用SCI-E和SSCI数据库进行检索,从发文趋势、研究力量布局、研究主题及演变规律、前沿发展态势探测方面进行分析。结果显示:关于大叶茶...为揭示大叶茶(Camellia sinensis var.assamica,CSA)研究的热点及前沿,研究采用文献计量学的方法,利用SCI-E和SSCI数据库进行检索,从发文趋势、研究力量布局、研究主题及演变规律、前沿发展态势探测方面进行分析。结果显示:关于大叶茶的研究总发文量呈上升趋势,分为萌芽期(1976-2004年)、发展期(2005-2014年)和快速增长期(2015年至今)3个阶段;发文单位以安徽农业大学、中国科学院和云南农业大学为主,刊载相关研究论文最多的期刊为《Journal of Agricultural and Food Chemistry》;关键词分析中,Antioxidant(抗氧化)、Theacrine(苦茶碱)和Fermentation(发酵)等具有较高词频,Genome(基因组)和Metabolomics(代谢组学)兼具高频词和新兴词的特征,为研究的热点词汇;引文网络分析显示,全基因组遗传分析、茶树系统发生学研究、靶向/非靶向代谢组分析以及化合物差异化分析为研究前沿。研究结果为学者开展研究以及相关机构制定科技战略规划提供参考。展开更多
A novel meroterpenoid,euphoractone(1),was isolated from the extracts of the roots of Euphorbia fischeriana Steud.Its structure was determined by spectroscopic methods and X-ray crystallography.1 possesses an unusual e...A novel meroterpenoid,euphoractone(1),was isolated from the extracts of the roots of Euphorbia fischeriana Steud.Its structure was determined by spectroscopic methods and X-ray crystallography.1 possesses an unusual ent-abietane-phloroglucinol skeleton.The plausible biosynthetic pathway for 1 was proposed.1 showed inhibitory activities against human lung cancer H23 and H460 cells with the IC_(50)values of 21.07±3.54 and 20.91±4.07 μmol/L.展开更多
基金supported by Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(CIFMSNos.2021-1-I2M-049 and 2018-I2M-1-001,China)+1 种基金the Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences(Nos.2019RC330001 and 2021RC310002,China)National Natural Science Foundation of China(No.82090010)。
文摘Silicosis is a leading cause of occupational disease-related morbidity and mortality worldwide,but the molecular basis underlying its development remains unclear.An accumulating body of evidence supports gasdermin D(GSDMD)-mediated pyroptosis as a key component in the development of various pulmonary diseases.However,there is little experimental evidence connecting silicosis and GSDMD-driven pyroptosis.In this work,we investigated the role of GSDMD-mediated pyroptosis in silicosis.Single-cell RNA sequencing of healthy and silicosis human and murine lung tissues indicated that GSDMD-induced pyroptosis in macrophages was relevant to silicosis progression.Through microscopy we then observed morphological alterations of pyroptosis in macrophages treated with silica.Measurement of interleukin-1βrelease,lactic dehydrogenase activity,and real-time propidium iodide staining further revealed that silica induced pyroptosis of macrophages.Additionally,we verified that both canonical(caspase-1-mediated)and non-canonical(caspase-4/5/11-mediated)signaling pathways mediated silica-induced pyroptosis activation,in vivo and in vitro.Notably,Gsdmd knockout mice exhibited dramatically alleviated silicosis phenotypes,which highlighted the pivotal role of pyroptosis in this disease.Taken together,our results demonstrated that macrophages underwent GSDMD-dependent pyroptosis in silicosis and inhibition of this process could serve as a viable clinical strategy for mitigating silicosis.
基金The authors gratefully acknowledge the financial support through the Pre-research Projects in the Equipment Field(No.61400040404)the National Science Foundation of China(Nos.51775232 and 51505183)+2 种基金the Sichuan Entrepreneurship Talent Project(No.2019JDRC0051)the Science and Technology Development Plan Project of Jilin Province(No.20190201155JC)the Fundamental Research Funds for the Central Universities.
文摘The past decade has witnessed significant efforts in addressing the global metallic corrosion challenge,with a focus on avoiding or mitigating huge economic losses incurred by corrosion and on the development of protective coatings on metals.Herein,a synergistic anticorrosion coating with both superhydrophobicity and self-healing properties was reported,through a facile replica molding method by mixing the polyvinylidene fluoride(PVDF)matrix with nano-sized SiO2 particles and 2-mercaptobenzothiazole(MBT)loaded halloysites(HNTs).The surface exhibits robust self-cleaning behavior under harsh conditions and high liquid repellence to withstand the osmosis of corrosive ions.The self-healing performance of the coating,due to the introduction of MBT-loaded HNTs,enhances the anticorrosion capability,which is still valid once the protective layer is damaged.Potentiodynamic polarization(PDP)and Electrochemical Impedance Spectroscopy(EIS)measurements demonstrate that the synergetic effects in anticorrosion performances significantly enhance the long-term corrosion protection of metals.Hence,this type of dual-action coating may find unique applications in metal corrosion resistance where both super-repellency and self-healing properties are desired.
文摘为揭示大叶茶(Camellia sinensis var.assamica,CSA)研究的热点及前沿,研究采用文献计量学的方法,利用SCI-E和SSCI数据库进行检索,从发文趋势、研究力量布局、研究主题及演变规律、前沿发展态势探测方面进行分析。结果显示:关于大叶茶的研究总发文量呈上升趋势,分为萌芽期(1976-2004年)、发展期(2005-2014年)和快速增长期(2015年至今)3个阶段;发文单位以安徽农业大学、中国科学院和云南农业大学为主,刊载相关研究论文最多的期刊为《Journal of Agricultural and Food Chemistry》;关键词分析中,Antioxidant(抗氧化)、Theacrine(苦茶碱)和Fermentation(发酵)等具有较高词频,Genome(基因组)和Metabolomics(代谢组学)兼具高频词和新兴词的特征,为研究的热点词汇;引文网络分析显示,全基因组遗传分析、茶树系统发生学研究、靶向/非靶向代谢组分析以及化合物差异化分析为研究前沿。研究结果为学者开展研究以及相关机构制定科技战略规划提供参考。
基金financially supported by the National Natural Science Foundation of China(No.81673530)Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program(No.2017BT01Y036)。
文摘A novel meroterpenoid,euphoractone(1),was isolated from the extracts of the roots of Euphorbia fischeriana Steud.Its structure was determined by spectroscopic methods and X-ray crystallography.1 possesses an unusual ent-abietane-phloroglucinol skeleton.The plausible biosynthetic pathway for 1 was proposed.1 showed inhibitory activities against human lung cancer H23 and H460 cells with the IC_(50)values of 21.07±3.54 and 20.91±4.07 μmol/L.
