目的 探讨牛黄醒脑丸对阿尔茨海默病模型小鼠Bcl-2、caspase-3及C型-半胱氨酸天冬氨酸特异性蛋白酶-3（type C cysteine aspartate specific protease-3,C-caspase-3）表达的影响.方法 将50只APP/V717小鼠按随机区组分组法分为牛黄醒脑...目的 探讨牛黄醒脑丸对阿尔茨海默病模型小鼠Bcl-2、caspase-3及C型-半胱氨酸天冬氨酸特异性蛋白酶-3（type C cysteine aspartate specific protease-3,C-caspase-3）表达的影响.方法 将50只APP/V717小鼠按随机区组分组法分为牛黄醒脑丸高、中、低剂量组,模型组,阳性对照组;另选取10只C57BL/6小鼠为空白对照组.牛黄醒脑丸高、中、低剂量组小鼠分别灌胃牛黄醒脑丸水溶液142、71、35.5 mg/kg;阳性对照组灌胃多奈哌齐溶液10 mg/kg;空白对照组及模型组灌胃等体积生理盐水.1次/d,连续给药60 d.采用Morris水迷宫实验检测各组小鼠的学习记忆能力.采用ELISA法检测小鼠海马组织SOD、GSH-Px及CAT含量;采用免疫印迹法检测小鼠海马组织Bcl-2、caspase-3、C-caspase-3表达.利用TUNEL染色法检测各组小鼠海马区细胞凋亡情况.结果 与模型组比较,牛黄醒脑丸高、中、低剂量组小鼠第Ⅲ象限停留时间[（36.58±4.57）s、（32.46±4.25）s、（29.71±4.26）s比（25.48±3.91）s]延长,穿越平台次数[（5.82±0.87）次、（4.59±0.76）次、（3.96±0.75）次比（3.27±0.53）次]增加（P〈0.05）;牛黄醒脑丸高、中、低剂量组小鼠海马GSH-Px[（161.14±14.01）U/mg、（150.76±13.16）U/mg、（143.17±12.54）U/mg比（120.78±10.92）U/mg]、SOD[（14.25±1.82）U/mg、（11.17±1.65）U/mg、（7.24±1.02）U/mg比（3.12±0.31）U/mg]、CAT[（17.95±2.16）U/mg、（16.72±1.83）U/mg、（15.54±1.47）U/mg比（13.25±2.60）U/mg]水平升高（P〈0.05）;caspase-3[（1.13±0.13）、（1.25±0.15）、（1.41±0.17）比（1.49±0.20）]、C-caspase-3[（1.17±0.14）、（1.27±0.16）、（1.42±0.18）比（1.52±0.23）]表达降低,Bcl-2[（0.88±0.08）、（0.79±0.06）、（0.67±0.04）比（0.59±0.04）]表达升高（P〈0.05）.TUNEL染色结果表明,牛黄醒脑丸高、中、低剂量组仅观察到少量凋亡神经元.结论 牛黄醒脑丸可调节阿尔茨海默病模型小鼠海马组�展开更多
Since its successful launch on October 1 this past year, Chang’e 2 hasoperated in space for more than 300days and completed its preset lunar exploration mission without fault. At 17:10 on June 9 this year, Chang’e 2...Since its successful launch on October 1 this past year, Chang’e 2 hasoperated in space for more than 300days and completed its preset lunar exploration mission without fault. At 17:10 on June 9 this year, Chang’e 2 left its lunarorbit,flying to the Lagrangian Point L2 to explore deep space beyond the moon. This pioneering trip from the moon shall lay the foundation for China’s next step, the Mars exploration program. At 23:27展开更多
文摘Since its successful launch on October 1 this past year, Chang’e 2 hasoperated in space for more than 300days and completed its preset lunar exploration mission without fault. At 17:10 on June 9 this year, Chang’e 2 left its lunarorbit,flying to the Lagrangian Point L2 to explore deep space beyond the moon. This pioneering trip from the moon shall lay the foundation for China’s next step, the Mars exploration program. At 23:27
