A set of 132 accessions of commonmillet, Panicum miliaceum L., from12 provinces of China were assessed for endosperm starch type （waxy or non-waxy） using I2-KI staining, amylose and amylopectin contents using the du...A set of 132 accessions of commonmillet, Panicum miliaceum L., from12 provinces of China were assessed for endosperm starch type （waxy or non-waxy） using I2-KI staining, amylose and amylopectin contents using the dual-wavelength colorimetric method, and genotype of Waxygenes conditioning amylose content by gene sequencing. Endosperm starch content varied from 57.69% to 74.70%, while the amylose and amylopectin contents of the starch ranged from 0 to 23.29% and from 41.99% to 70.24%, respectively. Sequencing two Wx genes, including Wx-L （intron 5-exon 7 and intron 8–9） andWx-S （exon 9–intron 10） revealed several polymorphisms （S0, S？15, LC, LF, LY）. Marker M5-R11 linking to the Wx-S gene may be used to discriminate waxy common millet accessions from non-waxy ones. Among the 132 accessions, 68 with the S？15 allele had waxy endosperm starch with the amylose content range 0–2.58% and 64 accessions with the S0 allele had non-waxy endosperm starch with amylose content range 3.94%–23.29%. Five genotypes including So15/LF （45% of the accessions）, S0/LF （25%）, S0/LY （12%）, S0/LC （11%）, and S？15/LY （7%） were identified. Six new SNPs were detected at the Wx-L locus. These results will facilitate common millet breeding, especially of cultivars free of amylose.展开更多
In situ bioprinting is promising for developing scaffolds directly on defect models in operating rooms,which provides a new strategy for in situ tissue regeneration.However,due to the limitation of existing in situ bi...In situ bioprinting is promising for developing scaffolds directly on defect models in operating rooms,which provides a new strategy for in situ tissue regeneration.However,due to the limitation of existing in situ biofabrication technologies including printing depth and suitable bioinks,bioprinting scaffolds in deep dermal or extremity injuries remains a grand challenge.Here,we present an in vivo scaffold fabrication approach by minimally invasive bioprinting electroactive hydrogel scaffolds to promote in situ tissue regeneration.The minimally invasive bioprinting system consists of a ferromagnetic soft catheter robot for extrusion,a digital laparoscope for in situ monitoring,and a Veress needle for establishing a pneumoperitoneum.After 3D reconstruction of the defects with computed tomography,electroactive hydrogel scaffolds are printed within partial liver resection of live rats,and in situ tissue regeneration is achieved by promoting the proliferation,migration,and differentiation of cells and maintaining liver function in vivo.展开更多
To investigate the role of pre-twins in Mg alloy sheets during warm planar deformation, the stretch forming is conducted at 200 ℃. Results suggest the formability of the pre-twinned AZ31 Mg alloy sheet is enhanced to...To investigate the role of pre-twins in Mg alloy sheets during warm planar deformation, the stretch forming is conducted at 200 ℃. Results suggest the formability of the pre-twinned AZ31 Mg alloy sheet is enhanced to 11.30 mm. The mechanisms for the improved formability and the deformation behaviors during the planar stretch forming are systematically investigated based on the planar stress states. The Schmid factor for deformation mechanisms are calculated, the results reveal that planar stress states extremely affect the Schmid factor for {10-12}twinning. The detwinning is activated and the prismatic slip is enhanced in the pre-twinned sheet, especially under the planar extension stress state in the outer region. Consequently, the thickness-direction strain is accommodated better. The dynamic recrystallization(DRX) type is continuous DRX(CDRX) regardless of the planar stress state. However, the CDRX degree is greater under the planar extension stress state.Some twin lattices deviate from the perfect {10-12} twinning relation due to the planar compression stress state and the CDRX. The basal texture is weakened when the planar stress state tends to change the texture components.展开更多
Introduction Lynch syndrome(LS)is an autosomal dominant condition caused by pathogenic variants in mismatch repair(MMR)genes.LS is associated with an 80%lifetime risk for colorectal cancer(CRC);it is also characterize...Introduction Lynch syndrome(LS)is an autosomal dominant condition caused by pathogenic variants in mismatch repair(MMR)genes.LS is associated with an 80%lifetime risk for colorectal cancer(CRC);it is also characterized by extracolonic tumors,including endometrial,stomach,or ovarian cancer[1,2].展开更多
In recent years, unexpected outbreaks of infectious diseases caused by emerging and re-emerging viruses have become more frequent, which is possibly due to environmental changes. These outbreaks result in the loss of ...In recent years, unexpected outbreaks of infectious diseases caused by emerging and re-emerging viruses have become more frequent, which is possibly due to environmental changes. These outbreaks result in the loss of life and economic hardship. Vaccines and therapeutics should be developed for the prevention and treatment of infectious diseases. In this review, we summarize and discuss the latest progress in the development of small-molecule viral inhibitors against highly pathogenic coronaviruses, including severe acute respiratory syndrome coronavirus and Middle East respiratory syndrome coronavirus, Ebola virus, and Zika virus. These viruses can interfere with the specific steps of viral life cycle by blocking the binding between virus and host cells, disrupting viral endocytosis, disturbing membrane fusion, and interrupting viral RNA replication and translation, thereby demonstrating potent therapeutic effect against various emerging and re-emerging viruses. We also discuss some general strategies for developing small-molecule viral inhibitors.展开更多
基金supported by the National Natural Science Foundation of China(31271791)Research Project supported by Shanxi Scholarship Council of China(2016-066)+4 种基金the Subject Construction Project of Anhui Academy of Agricultural Sciences(16A0102)China Agriculture Research System(CARS-06-13.5-A16)the Key Research and Development Program(General Project)(Agriculture)of Shanxi Province of China(201603D221003-5)the Special Program of Crop Germplasm Resources Protection and Utilization of Ministry of Agriculture of the People's Republic of China(2017NWB036-21)the Subplatform Project of National Proso Millet Germplasm Resources of China(NICGR2017-027)
文摘A set of 132 accessions of commonmillet, Panicum miliaceum L., from12 provinces of China were assessed for endosperm starch type （waxy or non-waxy） using I2-KI staining, amylose and amylopectin contents using the dual-wavelength colorimetric method, and genotype of Waxygenes conditioning amylose content by gene sequencing. Endosperm starch content varied from 57.69% to 74.70%, while the amylose and amylopectin contents of the starch ranged from 0 to 23.29% and from 41.99% to 70.24%, respectively. Sequencing two Wx genes, including Wx-L （intron 5-exon 7 and intron 8–9） andWx-S （exon 9–intron 10） revealed several polymorphisms （S0, S？15, LC, LF, LY）. Marker M5-R11 linking to the Wx-S gene may be used to discriminate waxy common millet accessions from non-waxy ones. Among the 132 accessions, 68 with the S？15 allele had waxy endosperm starch with the amylose content range 0–2.58% and 64 accessions with the S0 allele had non-waxy endosperm starch with amylose content range 3.94%–23.29%. Five genotypes including So15/LF （45% of the accessions）, S0/LF （25%）, S0/LY （12%）, S0/LC （11%）, and S？15/LY （7%） were identified. Six new SNPs were detected at the Wx-L locus. These results will facilitate common millet breeding, especially of cultivars free of amylose.
基金supported by the National Natural Science Foundation of China(52173280,51820105008,82173315,82072068,81773104,81873931,and 81974382)the Technical Innovation Major Foundation Program of Hubei Province(2018ACA136)+1 种基金the Integrated Innovative Team for Major Human Diseases Program of Tongji Medical College,HUSTthe Academic Doctor-Supporting Program of Tongji Medical College,HUST.
文摘In situ bioprinting is promising for developing scaffolds directly on defect models in operating rooms,which provides a new strategy for in situ tissue regeneration.However,due to the limitation of existing in situ biofabrication technologies including printing depth and suitable bioinks,bioprinting scaffolds in deep dermal or extremity injuries remains a grand challenge.Here,we present an in vivo scaffold fabrication approach by minimally invasive bioprinting electroactive hydrogel scaffolds to promote in situ tissue regeneration.The minimally invasive bioprinting system consists of a ferromagnetic soft catheter robot for extrusion,a digital laparoscope for in situ monitoring,and a Veress needle for establishing a pneumoperitoneum.After 3D reconstruction of the defects with computed tomography,electroactive hydrogel scaffolds are printed within partial liver resection of live rats,and in situ tissue regeneration is achieved by promoting the proliferation,migration,and differentiation of cells and maintaining liver function in vivo.
基金the Central Government Guided Local Science and Technology Development Projects (YDZJSX2021A010)China Postdoctoral Science Foundation (No.2022M710541)+5 种基金the National Natural Science Foundation of China (51704209,52274397,U1810208)the Projects of International Cooperation in Shanxi (201803D421086)Shanxi Province Patent Promotion Implementation Fund (20200718)Research Project Supported by Shanxi Scholarship Council of China (2022-038)Science and Technology Major Project of Shanxi Province (20191102008,20191102007,20181101008)Taishan Scholars Project Special Fund (2021)。
文摘To investigate the role of pre-twins in Mg alloy sheets during warm planar deformation, the stretch forming is conducted at 200 ℃. Results suggest the formability of the pre-twinned AZ31 Mg alloy sheet is enhanced to 11.30 mm. The mechanisms for the improved formability and the deformation behaviors during the planar stretch forming are systematically investigated based on the planar stress states. The Schmid factor for deformation mechanisms are calculated, the results reveal that planar stress states extremely affect the Schmid factor for {10-12}twinning. The detwinning is activated and the prismatic slip is enhanced in the pre-twinned sheet, especially under the planar extension stress state in the outer region. Consequently, the thickness-direction strain is accommodated better. The dynamic recrystallization(DRX) type is continuous DRX(CDRX) regardless of the planar stress state. However, the CDRX degree is greater under the planar extension stress state.Some twin lattices deviate from the perfect {10-12} twinning relation due to the planar compression stress state and the CDRX. The basal texture is weakened when the planar stress state tends to change the texture components.
基金supported by the National Natural Science Foundation of China Programs[82202617 and 82072068]the Technical Innovation Major Foundation Program of Hubei Province[2022BCA013]and the Natural Science Foundation of Hubei Province[2021CFB083].
文摘Introduction Lynch syndrome(LS)is an autosomal dominant condition caused by pathogenic variants in mismatch repair(MMR)genes.LS is associated with an 80%lifetime risk for colorectal cancer(CRC);it is also characterized by extracolonic tumors,including endometrial,stomach,or ovarian cancer[1,2].
文摘In recent years, unexpected outbreaks of infectious diseases caused by emerging and re-emerging viruses have become more frequent, which is possibly due to environmental changes. These outbreaks result in the loss of life and economic hardship. Vaccines and therapeutics should be developed for the prevention and treatment of infectious diseases. In this review, we summarize and discuss the latest progress in the development of small-molecule viral inhibitors against highly pathogenic coronaviruses, including severe acute respiratory syndrome coronavirus and Middle East respiratory syndrome coronavirus, Ebola virus, and Zika virus. These viruses can interfere with the specific steps of viral life cycle by blocking the binding between virus and host cells, disrupting viral endocytosis, disturbing membrane fusion, and interrupting viral RNA replication and translation, thereby demonstrating potent therapeutic effect against various emerging and re-emerging viruses. We also discuss some general strategies for developing small-molecule viral inhibitors.
