Objective:To investigate the potential mechanisms of electroacupuncture(EA)to prevent ischemic stroke.Methods:The method of middle cerebral artery occlusion(MCAO)was employed to establish a rat model of ischemic strok...Objective:To investigate the potential mechanisms of electroacupuncture(EA)to prevent ischemic stroke.Methods:The method of middle cerebral artery occlusion(MCAO)was employed to establish a rat model of ischemic stroke.Seventy-eight Sprague-Dawley rats were divided into the sham group,MCAO+EA control(EC)group,and MCAO+EA(EA)group according to a random number table(n=26 per group).EA was applied to the acupoints of Baihui(DU 20)and Shenting(DU 24)5 min and 6 h,respectively after the onset of MCAO for 30 min.Rats in the sham and EC groups received only light isoflurane anesthesia for 30 min after MCAO.The neuroprotective effects of EA were evaluated by rota-rod test,neurological deficit scores and infarct volumes.Additionally,Nissl staining and immunostaining were performed to examine brain damage,rod formation,cellular apoptosis,and neuronal loss induced by ischemia.The activities of caspase-3,and expression levels of cofilin and p-cofilin in mitochondria and cytoplasm after ischemic injury were determined by Western blot.Results:Compared with the EC group,EA significantly improved neuromotor function and cognitive ability after ischemic stroke(P<0.05 or P<0.01).Therapeutic use of EA also resulted in a significant decrease of cofilin rod formation and microtubule-associated protein-2(MAP2)degradation in the cortical penumbra area compared with the EC rats(P<0.01).Furthermore,Western blot analysis showed that EA stimulation significantly inhibited mitochondrial translocation of cofilin and caspase-3 cleavage(P<0.05 or P<0.01).Additionally,brain damage(infarct volume and neuropathy),cellular apoptosis and neuronal loss induced by ischemia were remarkably suppressed by EA in the cortical penumbra of rats(P<0.05 or P<0.01).Conclusion:EA treatment after ischemic stroke may attenuate ischemic brain injury and cellular apoptosis through the regulation of mitochondrial translocation of cofilin,a novel mechanism of EA therapy.展开更多
Background：Diabetes mellitus （DM） remains a major health problem worldwide.Several clinical trials have shown the superiority of the Traditional Chinese Medicine in delaying or reversing the development and progres...Background：Diabetes mellitus （DM） remains a major health problem worldwide.Several clinical trials have shown the superiority of the Traditional Chinese Medicine in delaying or reversing the development and progression of DM.This study aimed to evaluate the efficacy of Jinlida （JLD） granule,a Chinese herbal recipe,in the treatment of impaired glucose tolerance （IGT） and its effect on the prevention of DM.Methods：Sixty-five IGT patients were randomized to receive one bag of JLD granules three times daily （JLD group,n =34） or no drug intervention （control group,n =31） for 12 weeks.Oral glucose tolerance test,glycated hemoglobin A1c （HbA1c）,body mass index,blood lipids levels,fasting insulin,and insulin resistance calculated using homeostatic model assessment （HOMA-IR） of all the patients were observed and compared before and after the treatment.Results：Sixty-one participants completed the trial （32 in JLD group and 29 in the control group）.There were statistically significant decreases in HbA1c （P 〈 0.001）,2-h plasma glucose （P 〈 0.001）,and HOMA-IR （P =0.029） in JLD group compared with the control group after 12 weeks of treatment.After 12 weeks of treatment,two （6.9%） patients returned to normal blood glucose,and five （17.2%） patients turned into DM in control group,while in the JLD group,14 （43.8%） returned to normal blood glucose and 2 （6.2%） turned into DM.There was a significant difference in the number of subjects who had normal glucose at the end of the study between two groups （P =0.001）.Conclusions：JLD granule effectively improved glucose control,increased the conversion of IGT to normal glucose,and improved the insulin resistance in patients with IGT.This Chinese herbal medicine may have a clinical value for IGT.展开更多
目的探讨脑梗死和短暂性脑缺血发作患者外周血红细胞分布宽度(RDW)、中性粒细胞与淋巴细胞比值(NLR)的变化及之间的相关性。方法选取华北理工大学附属医院神经内科2010年5月～2016年7月收治的630例首发的急性脑梗死患者作为脑梗死组,16...目的探讨脑梗死和短暂性脑缺血发作患者外周血红细胞分布宽度(RDW)、中性粒细胞与淋巴细胞比值(NLR)的变化及之间的相关性。方法选取华北理工大学附属医院神经内科2010年5月～2016年7月收治的630例首发的急性脑梗死患者作为脑梗死组,162例首发的短暂性脑缺血发作患者作为短暂性脑缺血发作组,即TIA组,另选取150例健康体检者为对照组,比较各组患者外周血RDW,NLR,白细胞计数,血液流变学相关指标的水平,分析各组患者RDW与NLR之间的相关性。结果①急性脑梗死组水平高于TIA组及对照组[刚性指数4.18±0.49 vs 3.89±0.51和3.74±0.40,红细胞聚集指数4.91±0.59 vs 4.69±0.61和4.12±0.54,NLR 2.38±1.19 vs 2.01±1.00和1.36±0.85,白细胞计数(7.23±1.06 vs 6.42±0.79和5.12±0.82)×10~9/L,RDW 13.44%±0.59%vs 12.73%±0.58%和11.64%±0.51%],变形指数低于TIA组及对照组[0.66±0.05 vs 0.73±0.05和0.77±0.06],差异均有统计学意义(F=8.952～129.849,49.258,均P<0.001);②TIA组RDW,红细胞聚集指数,白细胞计数及NLR水平均高于对照组,差异均有统计学意义(P<0.001);③脑梗死组和TIA组中RDW与NLR呈正相关(r=0.73,P<0.01;r=0.63,P<0.01),但对照组RDW与NLR不相关(r=0.21,P=0.149)。结论脑梗死和短暂性脑缺血发作患者外周血RDW与NLR呈正相关,RDW可以为脑梗死和短暂性脑缺血发作患者的炎症状态起到提示作用。展开更多
The contactin-associated protein （Caspr） family participates in nerve excitation and conduction, and neurotransmitter release in myelinated axons. We analyzed the structures and functions of the Caspr family- CNTNA...The contactin-associated protein （Caspr） family participates in nerve excitation and conduction, and neurotransmitter release in myelinated axons. We analyzed the structures and functions of the Caspr family- CNTNAP1 （Casprl）, CNTNAP2 （Caspr2）, CNTNAP3 （Caspr3）, CNTNAP4 （Caspr4） and CNTNAP5 （Caspr5）, Casprl-5 is not only involved in the formation of myelinated axons, but also participates in maintaining the stability of adjacent connections. Casprl participates in the formation, differentiation, and proliferation of neurons and astrocytes, and in motor control and cognitive function. We also analyzed the relationship between the Caspr family and neurodegenerative diseases, multiple sclerosis, and autoimmune encephalitis. However, the effects of Caspr on disease course and prognosis remain poorly understood. The effects of Caspr on disease diagnosis and treatment need further investigation.展开更多
Background: Coronary heart disease(CHD) is characterized by arterial wall inflammation and matrix degradation. Matrix metalloproteinase(MMP)-22 and-29 and pro-inflammatory cytokine interleukin-18(IL18) are present in ...Background: Coronary heart disease(CHD) is characterized by arterial wall inflammation and matrix degradation. Matrix metalloproteinase(MMP)-22 and-29 and pro-inflammatory cytokine interleukin-18(IL18) are present in human hearts. IL18 may regulate MMP-22 and-29 expression, which may correlate with CHD progression. Methods and results: Immunoblot analysis showed that IL18 induced MMP-22 expression in human aortic smooth muscle cells. The Mann Whitney test from a prospective study of 194 CHD patients and 68 non-CHD controls demonstrated higher plasma levels of IL18, MMP-22 and-29 in CHD patients than in the controls. A logistic regression test suggested that plasma IL18(odds ratio(OR)=1.131, P=0.007), MMP-22(OR=1.213, P=0.040), and MMP-29(OR=1.198, P=0.033) were independent risk factors of CHD. Pearson's correlation test showed that IL18(coefficient(r)=0.214, P=0.045; r=0.246, P=0.031) and MMP-22(r=0.273, P=0.006; r=0.286, P=0.012) were associated with the Gensini score before and after adjusting for potential confounding factors. The multivariate Pearson's correlation test showed that plasma MMP-22 levels correlated positively with high-sensitive-C-reactive protein(hs-CRP)(r=0.167, P=0.023), and MMP-29 levels correlated negatively with triglyceride(r=-0.169, P=0.018). Spearman's correlation test indicated that plasma IL18 levels associated positively with plasma MMP-22(r=0.845, P<0.001) and MMP-29(r=0.548, P<0.001). Conclusions: Our observations suggest that IL18, MMP-22 and-29 serve as biomarkers and independent risk factors of CHD. Increased systemic IL18 in CHD patients may contribute to elevated plasma MMP-22 and-29 levels in these patients.展开更多
To investigate the influence of electric parameters and slag system on the solidification quality of electroslag ingot during electroslag remelting,different power supply modes,current strengths and remelting slag sys...To investigate the influence of electric parameters and slag system on the solidification quality of electroslag ingot during electroslag remelting,different power supply modes,current strengths and remelting slag systems were used to conduct electroslag remelting experiments on 304L austenitic stainless steel,and the macrostructure of electroslag ingots was analyzed.The results indicate that the depth of the metal pool decreases with the reduction of remelting frequency in the low frequency power supply mode.The effects of different power supply modes,such as low-frequency,direct current straight polarity(DCSP),and direct current reverse polarity(DCRP),on reducing the depth of the metal pool increase in that order.By reducing the remelting current strength in the same power supply mode,the depth of metal pool is reduced.When compared to the binary slag system of 70%CaF2+30%Al2O3,the ternary slag system of 60%CaF2+20%Al2O3+20%CaO is more effective in reducing the depth of the metal pool during remelting.Utilizing the 60%CaF2+20%Al2O3+20%CaO ternary slag system results in a shallower and flatter metal pool,with columnar crystal growth occurring closer to the axial crystal.This effect is observed for both low frequency and direct current(DC)power supply modes.展开更多
基金Supported by the National Natural Science Foundation of China(No.81804175 and 81803883)Natural Science Foundation of Fujian Province(No.2019J01497)+2 种基金Foundation of Fujian Key Laboratory of Rehabilitation Technology(No.KF2019006)Special Research Project of National Clinical Research Base of Traditional Chinese Medicine(No.JDZX2019040)Educational Research Project for Young and Middle-Aged Teachers of Education Bureau of Fujian Province(No.JT180216)。
文摘Objective:To investigate the potential mechanisms of electroacupuncture(EA)to prevent ischemic stroke.Methods:The method of middle cerebral artery occlusion(MCAO)was employed to establish a rat model of ischemic stroke.Seventy-eight Sprague-Dawley rats were divided into the sham group,MCAO+EA control(EC)group,and MCAO+EA(EA)group according to a random number table(n=26 per group).EA was applied to the acupoints of Baihui(DU 20)and Shenting(DU 24)5 min and 6 h,respectively after the onset of MCAO for 30 min.Rats in the sham and EC groups received only light isoflurane anesthesia for 30 min after MCAO.The neuroprotective effects of EA were evaluated by rota-rod test,neurological deficit scores and infarct volumes.Additionally,Nissl staining and immunostaining were performed to examine brain damage,rod formation,cellular apoptosis,and neuronal loss induced by ischemia.The activities of caspase-3,and expression levels of cofilin and p-cofilin in mitochondria and cytoplasm after ischemic injury were determined by Western blot.Results:Compared with the EC group,EA significantly improved neuromotor function and cognitive ability after ischemic stroke(P<0.05 or P<0.01).Therapeutic use of EA also resulted in a significant decrease of cofilin rod formation and microtubule-associated protein-2(MAP2)degradation in the cortical penumbra area compared with the EC rats(P<0.01).Furthermore,Western blot analysis showed that EA stimulation significantly inhibited mitochondrial translocation of cofilin and caspase-3 cleavage(P<0.05 or P<0.01).Additionally,brain damage(infarct volume and neuropathy),cellular apoptosis and neuronal loss induced by ischemia were remarkably suppressed by EA in the cortical penumbra of rats(P<0.05 or P<0.01).Conclusion:EA treatment after ischemic stroke may attenuate ischemic brain injury and cellular apoptosis through the regulation of mitochondrial translocation of cofilin,a novel mechanism of EA therapy.
文摘Background：Diabetes mellitus （DM） remains a major health problem worldwide.Several clinical trials have shown the superiority of the Traditional Chinese Medicine in delaying or reversing the development and progression of DM.This study aimed to evaluate the efficacy of Jinlida （JLD） granule,a Chinese herbal recipe,in the treatment of impaired glucose tolerance （IGT） and its effect on the prevention of DM.Methods：Sixty-five IGT patients were randomized to receive one bag of JLD granules three times daily （JLD group,n =34） or no drug intervention （control group,n =31） for 12 weeks.Oral glucose tolerance test,glycated hemoglobin A1c （HbA1c）,body mass index,blood lipids levels,fasting insulin,and insulin resistance calculated using homeostatic model assessment （HOMA-IR） of all the patients were observed and compared before and after the treatment.Results：Sixty-one participants completed the trial （32 in JLD group and 29 in the control group）.There were statistically significant decreases in HbA1c （P 〈 0.001）,2-h plasma glucose （P 〈 0.001）,and HOMA-IR （P =0.029） in JLD group compared with the control group after 12 weeks of treatment.After 12 weeks of treatment,two （6.9%） patients returned to normal blood glucose,and five （17.2%） patients turned into DM in control group,while in the JLD group,14 （43.8%） returned to normal blood glucose and 2 （6.2%） turned into DM.There was a significant difference in the number of subjects who had normal glucose at the end of the study between two groups （P =0.001）.Conclusions：JLD granule effectively improved glucose control,increased the conversion of IGT to normal glucose,and improved the insulin resistance in patients with IGT.This Chinese herbal medicine may have a clinical value for IGT.
文摘目的探讨脑梗死和短暂性脑缺血发作患者外周血红细胞分布宽度(RDW)、中性粒细胞与淋巴细胞比值(NLR)的变化及之间的相关性。方法选取华北理工大学附属医院神经内科2010年5月～2016年7月收治的630例首发的急性脑梗死患者作为脑梗死组,162例首发的短暂性脑缺血发作患者作为短暂性脑缺血发作组,即TIA组,另选取150例健康体检者为对照组,比较各组患者外周血RDW,NLR,白细胞计数,血液流变学相关指标的水平,分析各组患者RDW与NLR之间的相关性。结果①急性脑梗死组水平高于TIA组及对照组[刚性指数4.18±0.49 vs 3.89±0.51和3.74±0.40,红细胞聚集指数4.91±0.59 vs 4.69±0.61和4.12±0.54,NLR 2.38±1.19 vs 2.01±1.00和1.36±0.85,白细胞计数(7.23±1.06 vs 6.42±0.79和5.12±0.82)×10~9/L,RDW 13.44%±0.59%vs 12.73%±0.58%和11.64%±0.51%],变形指数低于TIA组及对照组[0.66±0.05 vs 0.73±0.05和0.77±0.06],差异均有统计学意义(F=8.952～129.849,49.258,均P<0.001);②TIA组RDW,红细胞聚集指数,白细胞计数及NLR水平均高于对照组,差异均有统计学意义(P<0.001);③脑梗死组和TIA组中RDW与NLR呈正相关(r=0.73,P<0.01;r=0.63,P<0.01),但对照组RDW与NLR不相关(r=0.21,P=0.149)。结论脑梗死和短暂性脑缺血发作患者外周血RDW与NLR呈正相关,RDW可以为脑梗死和短暂性脑缺血发作患者的炎症状态起到提示作用。
基金supported by a grant from the Instructional Program of Science and Technology Bureau of Wuxi City of China,No.0302B010507130039PB
文摘The contactin-associated protein （Caspr） family participates in nerve excitation and conduction, and neurotransmitter release in myelinated axons. We analyzed the structures and functions of the Caspr family- CNTNAP1 （Casprl）, CNTNAP2 （Caspr2）, CNTNAP3 （Caspr3）, CNTNAP4 （Caspr4） and CNTNAP5 （Caspr5）, Casprl-5 is not only involved in the formation of myelinated axons, but also participates in maintaining the stability of adjacent connections. Casprl participates in the formation, differentiation, and proliferation of neurons and astrocytes, and in motor control and cognitive function. We also analyzed the relationship between the Caspr family and neurodegenerative diseases, multiple sclerosis, and autoimmune encephalitis. However, the effects of Caspr on disease course and prognosis remain poorly understood. The effects of Caspr on disease diagnosis and treatment need further investigation.
基金supported by the University of Science and Technology Innovation Team of Henan(No.14IRTSTHN018)the Science and Technology Talents Team Construction Program of Zhengzhou City Science and Technology Talents(No.131PLJRC670),Chinathe National Institutes of Health(Nos.HL60942 and HL123568),USA
文摘Background: Coronary heart disease(CHD) is characterized by arterial wall inflammation and matrix degradation. Matrix metalloproteinase(MMP)-22 and-29 and pro-inflammatory cytokine interleukin-18(IL18) are present in human hearts. IL18 may regulate MMP-22 and-29 expression, which may correlate with CHD progression. Methods and results: Immunoblot analysis showed that IL18 induced MMP-22 expression in human aortic smooth muscle cells. The Mann Whitney test from a prospective study of 194 CHD patients and 68 non-CHD controls demonstrated higher plasma levels of IL18, MMP-22 and-29 in CHD patients than in the controls. A logistic regression test suggested that plasma IL18(odds ratio(OR)=1.131, P=0.007), MMP-22(OR=1.213, P=0.040), and MMP-29(OR=1.198, P=0.033) were independent risk factors of CHD. Pearson's correlation test showed that IL18(coefficient(r)=0.214, P=0.045; r=0.246, P=0.031) and MMP-22(r=0.273, P=0.006; r=0.286, P=0.012) were associated with the Gensini score before and after adjusting for potential confounding factors. The multivariate Pearson's correlation test showed that plasma MMP-22 levels correlated positively with high-sensitive-C-reactive protein(hs-CRP)(r=0.167, P=0.023), and MMP-29 levels correlated negatively with triglyceride(r=-0.169, P=0.018). Spearman's correlation test indicated that plasma IL18 levels associated positively with plasma MMP-22(r=0.845, P<0.001) and MMP-29(r=0.548, P<0.001). Conclusions: Our observations suggest that IL18, MMP-22 and-29 serve as biomarkers and independent risk factors of CHD. Increased systemic IL18 in CHD patients may contribute to elevated plasma MMP-22 and-29 levels in these patients.
基金financially supported by the National Natural Science Foundation of China(Grant Nos.52074002,52174289)Natural Science Foundation of Anhui Province(Grant No.2208085J37)。
文摘To investigate the influence of electric parameters and slag system on the solidification quality of electroslag ingot during electroslag remelting,different power supply modes,current strengths and remelting slag systems were used to conduct electroslag remelting experiments on 304L austenitic stainless steel,and the macrostructure of electroslag ingots was analyzed.The results indicate that the depth of the metal pool decreases with the reduction of remelting frequency in the low frequency power supply mode.The effects of different power supply modes,such as low-frequency,direct current straight polarity(DCSP),and direct current reverse polarity(DCRP),on reducing the depth of the metal pool increase in that order.By reducing the remelting current strength in the same power supply mode,the depth of metal pool is reduced.When compared to the binary slag system of 70%CaF2+30%Al2O3,the ternary slag system of 60%CaF2+20%Al2O3+20%CaO is more effective in reducing the depth of the metal pool during remelting.Utilizing the 60%CaF2+20%Al2O3+20%CaO ternary slag system results in a shallower and flatter metal pool,with columnar crystal growth occurring closer to the axial crystal.This effect is observed for both low frequency and direct current(DC)power supply modes.
