AIM: To study the anti-HBV effect of liposome-encapsulated matrine (Lip-M) in vitro and in vivo. METHODS: 2.2.15 cell line was cultured in vitro observe the effect of Lip-M and matrine on the secretion of HBsAg and HB...AIM: To study the anti-HBV effect of liposome-encapsulated matrine (Lip-M) in vitro and in vivo. METHODS: 2.2.15 cell line was cultured in vitro observe the effect of Lip-M and matrine on the secretion of HBsAg and HBeAg. The toxicity of Lip-M and matrine to 2.2.15 cell line was also studied by MTT method. In in vivo study, drug treatment experiment was carried out on the 13th day after ducks were infected with duck hepatitis B virus (DHBV). The ducks were randomly divided into 4 groups with 5-6 ducks in each group. Lip-M and matrine were given to DHBV-infected ducks respectively by gastric perfusion. Four groups were observed: group of Lip-M (20 mg/kg), group of Lip-M (10 mg/kg), group of matrine (20 mg/kg) and group of blank model. The drug was given once daily for 20 d continuously, and normal saline was used as control. The blood was drawn from the posterior tibial vein of all ducks before treatment (T0), after the medication for 5 (T5), 10 (T10), 15 (T15), 20 (T20) d and withdrawl of the drug for 3 d (P3). The serum samples were separated and stored at -70 ℃, DHBV-DNA was detected by the dot-blot hybridization. RESULTS: After addition of Lip-M and matrine to 2.2.15 cell line for eleven d, the median toxic concentration (TC50) of Lip-M and matrine was 7.29 mg/mL and 1.33 mg/mL respectively. The median concentration (IC50) of Lip-M to inhibit HBsAg and HBeAg expression was 0.078 mg/mL and 3.35 mg/mL respectively. The treatment index (TI) value of Lip-M for HBsAg and HBeAg was 93.46 and 2.17 respectively, better than that of matrine. The DHBV-infected duck model treatment test showed that the duck serum DHBV-DNA levels were markedly reduced in the group of Lip-M (20 mg/kg) after treated by gastric perfusion for 10, 15 and 20 d (0.43±0.22 vs 0.95±0.18, t = 4.70, P= 0.001<0.01.0.40±0.12 vs 0.95±0.18, t = 6.34, P= 0.000<0.01. 0.22±0.10 vs 0.95±0.18, t = 8.30, P= 0.000<0.01), compared to the group of matrine (20 mg/kg) (0.43±0.22 vs 0.79±0.19, t = 3.17, P= 0.01<0.05. 0.40±0.12 vs 0.73±0.24, t = 3.21, P=展开更多
目的分析针灸联合康复治疗应用于脑卒中急性期吞咽困难的疗效.方法将2019年7月—2020年12月于该院就诊的80例脑卒中急性期吞咽困难患者纳为研究对象,随机分为对照组40例和研究组40例.对照组采用康复治疗,研究组采用针灸联合康复治疗.对...目的分析针灸联合康复治疗应用于脑卒中急性期吞咽困难的疗效.方法将2019年7月—2020年12月于该院就诊的80例脑卒中急性期吞咽困难患者纳为研究对象,随机分为对照组40例和研究组40例.对照组采用康复治疗,研究组采用针灸联合康复治疗.对比评估两组临床治疗效果.结果两组总有效率比较,研究组为95.00%,明显高于对照组的77.50%,差异有统计学意义(P<0.05).两组治疗前的吞咽困难程度评分与经口摄食能力评分比较,差异无统计学意义(P>0.05);治疗后,研究组吞咽困难程度评分低于对照组,经口摄食能力评分高于对照组,组间差异有统计学意义(P<0.05).两组治疗前的Barthel指数评分、吞咽障碍特异性生活质量量表(swallowing quality of life,SWAL-QOL)评分比较,差异无统计学意义(P>0.05);治疗后,与对照组相比,研究组Barthel评分、SWAL-QOL评分均更高,组间差异有统计学意义(P<0.05).结论脑卒中急性期吞咽困难患者采用针灸联合康复治疗方案可取得理想的治疗效果,能够改善其吞咽困难程度及经口摄食能力,提升其日常生活能力及生存质量.展开更多
基金Supported by the Natural Science Foundation of Guangdong Province, No.B990353
文摘AIM: To study the anti-HBV effect of liposome-encapsulated matrine (Lip-M) in vitro and in vivo. METHODS: 2.2.15 cell line was cultured in vitro observe the effect of Lip-M and matrine on the secretion of HBsAg and HBeAg. The toxicity of Lip-M and matrine to 2.2.15 cell line was also studied by MTT method. In in vivo study, drug treatment experiment was carried out on the 13th day after ducks were infected with duck hepatitis B virus (DHBV). The ducks were randomly divided into 4 groups with 5-6 ducks in each group. Lip-M and matrine were given to DHBV-infected ducks respectively by gastric perfusion. Four groups were observed: group of Lip-M (20 mg/kg), group of Lip-M (10 mg/kg), group of matrine (20 mg/kg) and group of blank model. The drug was given once daily for 20 d continuously, and normal saline was used as control. The blood was drawn from the posterior tibial vein of all ducks before treatment (T0), after the medication for 5 (T5), 10 (T10), 15 (T15), 20 (T20) d and withdrawl of the drug for 3 d (P3). The serum samples were separated and stored at -70 ℃, DHBV-DNA was detected by the dot-blot hybridization. RESULTS: After addition of Lip-M and matrine to 2.2.15 cell line for eleven d, the median toxic concentration (TC50) of Lip-M and matrine was 7.29 mg/mL and 1.33 mg/mL respectively. The median concentration (IC50) of Lip-M to inhibit HBsAg and HBeAg expression was 0.078 mg/mL and 3.35 mg/mL respectively. The treatment index (TI) value of Lip-M for HBsAg and HBeAg was 93.46 and 2.17 respectively, better than that of matrine. The DHBV-infected duck model treatment test showed that the duck serum DHBV-DNA levels were markedly reduced in the group of Lip-M (20 mg/kg) after treated by gastric perfusion for 10, 15 and 20 d (0.43±0.22 vs 0.95±0.18, t = 4.70, P= 0.001<0.01.0.40±0.12 vs 0.95±0.18, t = 6.34, P= 0.000<0.01. 0.22±0.10 vs 0.95±0.18, t = 8.30, P= 0.000<0.01), compared to the group of matrine (20 mg/kg) (0.43±0.22 vs 0.79±0.19, t = 3.17, P= 0.01<0.05. 0.40±0.12 vs 0.73±0.24, t = 3.21, P=
文摘目的分析针灸联合康复治疗应用于脑卒中急性期吞咽困难的疗效.方法将2019年7月—2020年12月于该院就诊的80例脑卒中急性期吞咽困难患者纳为研究对象,随机分为对照组40例和研究组40例.对照组采用康复治疗,研究组采用针灸联合康复治疗.对比评估两组临床治疗效果.结果两组总有效率比较,研究组为95.00%,明显高于对照组的77.50%,差异有统计学意义(P<0.05).两组治疗前的吞咽困难程度评分与经口摄食能力评分比较,差异无统计学意义(P>0.05);治疗后,研究组吞咽困难程度评分低于对照组,经口摄食能力评分高于对照组,组间差异有统计学意义(P<0.05).两组治疗前的Barthel指数评分、吞咽障碍特异性生活质量量表(swallowing quality of life,SWAL-QOL)评分比较,差异无统计学意义(P>0.05);治疗后,与对照组相比,研究组Barthel评分、SWAL-QOL评分均更高,组间差异有统计学意义(P<0.05).结论脑卒中急性期吞咽困难患者采用针灸联合康复治疗方案可取得理想的治疗效果,能够改善其吞咽困难程度及经口摄食能力,提升其日常生活能力及生存质量.
