Soft tissue sealing around implants acts as a barrier between the alveolar bone and oral environment,protecting implants from the invasion of bacteria or external stimuli.In this work,magnesium(Mg)and zinc(Zn)are intr...Soft tissue sealing around implants acts as a barrier between the alveolar bone and oral environment,protecting implants from the invasion of bacteria or external stimuli.In this work,magnesium(Mg)and zinc(Zn)are introduced into titanium by plasma immersed ion implantation technology,and their effects on the behaviors of human gingival fibroblasts(HGFs)as well as the underlying mechanisms are investigated.Surface characterization confirms Mg and Zn exist on the surface in metallic and oxidized states.Contact angle test suggests that surface wettability of titanium changes after ion implantation and thus influences protein adsorption of surfaces.In vitro studies disclose that HGFs on Mg ion-implanted samples exhibit better adhesion and migration while cells on Zn ion-implanted samples have higher proliferation rate and amounts.The results of immunofluorescence staining and real-time reverse-transcriptase polymerase chain reaction(RT-PCR)suggest that Mg mainly regulates the motility and adhesion of HGFs through activating the MAPK signal pathway whereas Zn influences HGFs proliferation by triggering the TGF-βsignal pathway.The synergistic effect of Mg and Zn ions ensure that HGFs cultured on co-implanted samples possessed both high proliferation rate and motility,which are critical to soft tissue sealing of implants.展开更多
Neural stem cells(NSCs)in the spinal cord hold great potential for repair after spinal cord injury(SCI).The ependyma in the central canal(CC)region has been considered as the NSCs source in the spinal cord.However,the...Neural stem cells(NSCs)in the spinal cord hold great potential for repair after spinal cord injury(SCI).The ependyma in the central canal(CC)region has been considered as the NSCs source in the spinal cord.However,the ependyma function as NSCs after SCI is still under debate.We used Nestin as a marker to isolate potential NSCs and their immediate progeny,and characterized the cells before and after SCI by single-cell RNA-sequencing(scRNA-seq).We identified two subgroups of NSCs:the subgroup located within the CC cannot prime to active NSCs after SCI,while the subgroup located outside the CC were activated and exhibited the active NSCs properties after SCI.We demonstrated the comprehensive dynamic transcriptome of NSCs from quiescent to active NSCs after SCI.This study reveals that Nestin+cells outside CC were NSCs that activated upon SCI and may thus serve as endogenous NSCs for regenerative treatment of SCI in the future.展开更多
A flexible and transparent triboelectric nanogenerator （FT-TENG） has great potential for application in self-powered biosensor systems, electronic skin and wearable electronic devices. However, improving the output ...A flexible and transparent triboelectric nanogenerator （FT-TENG） has great potential for application in self-powered biosensor systems, electronic skin and wearable electronic devices. However, improving the output performance with little damage to its optical properties is challenging. Herein, we have developed an FT-TENG that has a well-ordered nest-like porous polydimethylsiloxane （NP-PDMS） film and graphene transparent electrodes. The NP-PDMS film with ordered pores is fabricated by hydrochloric acid etching of 500 nm sized ZnO spheres made of aggregated nanoparticles, having a light transmittance of 81.8% and a water contact angle of 118.62°. The FT-TENG based on the NP-PDMS film with a porosity of 12%, gives a maximum output of 271 V and 7.8 μA, which are respectively, 3.7 and 2.1-fold of those of a TENG with a flat PDMS film. The peak output power reaches 0.39 mW with a load resistance of 9.01 MΩ. The dielectric constant and effective thickness of the NP-PDMS film and the capacitance and charge transfer of the FT-TENG are systematically investigated. This work provides a novel and effective method to enhance the performance of FT-TENGs with little damage to their optical properties.展开更多
Attempt of developing bio-safety and functional layered double hydroxides(LDHs)modifed plasma electrolytic oxidation(PEO)coatings,has become a hotspot in the protection of magnesium(Mg)based biomedical implants.In the...Attempt of developing bio-safety and functional layered double hydroxides(LDHs)modifed plasma electrolytic oxidation(PEO)coatings,has become a hotspot in the protection of magnesium(Mg)based biomedical implants.In the present work,Mg-Fe LDH flms with different Fe contents were fabricated on PEO coating via a novel two-step method:frstly,the Fe OOH flms were prepared by immersing PEO sample in Fe^(2+)-containing solution,and then Mg-Fe LDH flms were formed by transforming the Fe OOH flms via a hydrothermal treatment in water.The highly-oriented LDH nano-sheets could enhance the anti-corrosion performance of PEO coating,which was proved by the results of electrochemical test,hydrogen evolution and corroded morphology.PEO/Mg-Fe LDH coatings showed a low hemolysis rate(less5%)than PEO coating.In addition,PEO/Mg-Fe LDH coatings were more favorable for cell adhesion and proliferation than PEO coating.Moreover,PEO/Mg-Fe LDH coatings showed good photothermal conversion property,which demonstrated the excellent rapid antibacterial effect under NIR light.In vitro culture of rat bone marrow stem cell(r BMSC)suggested that cells cultured in the extract of PEO/Mg-Fe LDH coatings had a better osteogenic activity.In vivo subcutaneous implantation test revealed that PEO/Mg-Fe LDH coatings exhibited good anti-corrosion and histocompatibility.展开更多
A real time PCR assay for the detection of Vibrio para-haemolyticus in seafood samples was developed using a novel specific target and a competitive internal ampli-fication control(IAC).The specificity of this assay w...A real time PCR assay for the detection of Vibrio para-haemolyticus in seafood samples was developed using a novel specific target and a competitive internal ampli-fication control(IAC).The specificity of this assay was evaluated using 390 bacterial strains including V.parahaemolyticus,and other strains belonging to Vibrio and non-Vibrio species.The real time PCR assay un-ambiguously distinguished V.parahaemolyticus with a detection sensitivity of 4.8 fg per PCR with purified genomic DNA or 1 CFU per reaction by counting V.parahaemolyticus colonies.The assays of avoiding interference demonstrated that,even in the presence of 2.1μg genomic DNA or 107 CFU background bacteria,V.parahaemolyticus could still be accurately detected.In addition,the IAC was used to indicate false-negative results,and lower than 94 copies of IAC per reaction had no influence on the detection limit.Ninety-six sea-food samples were tested,of which 58(60.4%)were positive,including 3 false negative results.Conse-quently,the real time PCR assay is effective for the rapid detection of V.parahaemotyticus contaminants in seafood.展开更多
Every year,millions of people travel to high-elevation regions(≥2500 m above sea level)for sightseeing,mountaineering,trekking,skiing or commercial and scientific research activities.As the largest and highest platea...Every year,millions of people travel to high-elevation regions(≥2500 m above sea level)for sightseeing,mountaineering,trekking,skiing or commercial and scientific research activities.As the largest and highest plateau of the world,the Qinghai-Tibet Plateau(QTP)is one of the most attractive tourist destinations because of its multiple splendid sceneries.High-altitude illnesses occur when the rate of ascent to a high altitude overcomes the ability of the individual to acclimatize.Chronic mountain sickness(CMS)is one of the most important high-altitude pathologies in most high-altitude regions over the world.展开更多
Light and phytohormone are external and internal cues that regulate plant growth and development throughout their life cycle.BBXs(B-box domain proteins)are a group of zinc finger proteins that not only directly govern...Light and phytohormone are external and internal cues that regulate plant growth and development throughout their life cycle.BBXs(B-box domain proteins)are a group of zinc finger proteins that not only directly govern the transcription of target genes but also associate with other factors to create a meticulous regulatory network to precisely regulate numerous aspects of growth and developmental processes in plants.Recent studies demonstrate that BBXs play pivotal roles in light-controlled plant growth and development.Besides,BBXs have been documented to regulate phytohormone-mediated physiological procedures.In this review,we summarize and highlight the multi-faced role of BBXs,with a focus in photomorphogenesis,photoperiodic flowering,shade avoidance,abiotic stress,and phytohormone-mediated growth and development in plant.展开更多
基金the National Natural Science Foundation of China(31971259,51831011,31870945)National Natural Science Foundation for Distinguished Young Scholars of China(51525207)+1 种基金Science and Technology Commission of Shanghai Municipality(18410760600,18YF1426900)International Partnership Program of Chinese Academy of Sciences(GJHZ1850)are acknowledged.
文摘Soft tissue sealing around implants acts as a barrier between the alveolar bone and oral environment,protecting implants from the invasion of bacteria or external stimuli.In this work,magnesium(Mg)and zinc(Zn)are introduced into titanium by plasma immersed ion implantation technology,and their effects on the behaviors of human gingival fibroblasts(HGFs)as well as the underlying mechanisms are investigated.Surface characterization confirms Mg and Zn exist on the surface in metallic and oxidized states.Contact angle test suggests that surface wettability of titanium changes after ion implantation and thus influences protein adsorption of surfaces.In vitro studies disclose that HGFs on Mg ion-implanted samples exhibit better adhesion and migration while cells on Zn ion-implanted samples have higher proliferation rate and amounts.The results of immunofluorescence staining and real-time reverse-transcriptase polymerase chain reaction(RT-PCR)suggest that Mg mainly regulates the motility and adhesion of HGFs through activating the MAPK signal pathway whereas Zn influences HGFs proliferation by triggering the TGF-βsignal pathway.The synergistic effect of Mg and Zn ions ensure that HGFs cultured on co-implanted samples possessed both high proliferation rate and motility,which are critical to soft tissue sealing of implants.
基金the National Natural Science Foundation of China(81891002 and 81891001)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA16040700)+1 种基金the National Key Research and Development Program of China(2017YFA0104701,2017YFA0104704 and 2016YFC1101501)Jiangsu Key Research and Development Program(BE2018664).
文摘Neural stem cells(NSCs)in the spinal cord hold great potential for repair after spinal cord injury(SCI).The ependyma in the central canal(CC)region has been considered as the NSCs source in the spinal cord.However,the ependyma function as NSCs after SCI is still under debate.We used Nestin as a marker to isolate potential NSCs and their immediate progeny,and characterized the cells before and after SCI by single-cell RNA-sequencing(scRNA-seq).We identified two subgroups of NSCs:the subgroup located within the CC cannot prime to active NSCs after SCI,while the subgroup located outside the CC were activated and exhibited the active NSCs properties after SCI.We demonstrated the comprehensive dynamic transcriptome of NSCs from quiescent to active NSCs after SCI.This study reveals that Nestin+cells outside CC were NSCs that activated upon SCI and may thus serve as endogenous NSCs for regenerative treatment of SCI in the future.
基金This work is supported by the National Natural Science Foundation of China （No. 51572040）, the National High-tech R＆D Program of China （No. 2015AA034801） and the large-scale equipment sharing fund of Chongqing University.
文摘A flexible and transparent triboelectric nanogenerator （FT-TENG） has great potential for application in self-powered biosensor systems, electronic skin and wearable electronic devices. However, improving the output performance with little damage to its optical properties is challenging. Herein, we have developed an FT-TENG that has a well-ordered nest-like porous polydimethylsiloxane （NP-PDMS） film and graphene transparent electrodes. The NP-PDMS film with ordered pores is fabricated by hydrochloric acid etching of 500 nm sized ZnO spheres made of aggregated nanoparticles, having a light transmittance of 81.8% and a water contact angle of 118.62°. The FT-TENG based on the NP-PDMS film with a porosity of 12%, gives a maximum output of 271 V and 7.8 μA, which are respectively, 3.7 and 2.1-fold of those of a TENG with a flat PDMS film. The peak output power reaches 0.39 mW with a load resistance of 9.01 MΩ. The dielectric constant and effective thickness of the NP-PDMS film and the capacitance and charge transfer of the FT-TENG are systematically investigated. This work provides a novel and effective method to enhance the performance of FT-TENGs with little damage to their optical properties.
基金fnancially supported by the National Key Research and Development Program of China(Nos.2017YFB0702600 and 2017YFB0702602)the National Natural Science Foundation of China(No.31771044)+1 种基金the Shanghai Committee of Science and Technology,China(Nos.20S31901200,18YF1426900 and 19JC1415500)the International Partnership Program of Chinese Academy of Sciences Grant(No.GJHZ1850)。
文摘Attempt of developing bio-safety and functional layered double hydroxides(LDHs)modifed plasma electrolytic oxidation(PEO)coatings,has become a hotspot in the protection of magnesium(Mg)based biomedical implants.In the present work,Mg-Fe LDH flms with different Fe contents were fabricated on PEO coating via a novel two-step method:frstly,the Fe OOH flms were prepared by immersing PEO sample in Fe^(2+)-containing solution,and then Mg-Fe LDH flms were formed by transforming the Fe OOH flms via a hydrothermal treatment in water.The highly-oriented LDH nano-sheets could enhance the anti-corrosion performance of PEO coating,which was proved by the results of electrochemical test,hydrogen evolution and corroded morphology.PEO/Mg-Fe LDH coatings showed a low hemolysis rate(less5%)than PEO coating.In addition,PEO/Mg-Fe LDH coatings were more favorable for cell adhesion and proliferation than PEO coating.Moreover,PEO/Mg-Fe LDH coatings showed good photothermal conversion property,which demonstrated the excellent rapid antibacterial effect under NIR light.In vitro culture of rat bone marrow stem cell(r BMSC)suggested that cells cultured in the extract of PEO/Mg-Fe LDH coatings had a better osteogenic activity.In vivo subcutaneous implantation test revealed that PEO/Mg-Fe LDH coatings exhibited good anti-corrosion and histocompatibility.
基金supported by grants from the Ministry of Sci-ence and Technology of China(Nos.2012AA101601 and 2011DFA31220)the National Natural Science Foundation of China(Grant Nos.31171690,30972485,31000779 and U1031003)the Science and Technology Commission of Shanghai Municipality(Nos.10DZ0503500 and 10142201300).
文摘A real time PCR assay for the detection of Vibrio para-haemolyticus in seafood samples was developed using a novel specific target and a competitive internal ampli-fication control(IAC).The specificity of this assay was evaluated using 390 bacterial strains including V.parahaemolyticus,and other strains belonging to Vibrio and non-Vibrio species.The real time PCR assay un-ambiguously distinguished V.parahaemolyticus with a detection sensitivity of 4.8 fg per PCR with purified genomic DNA or 1 CFU per reaction by counting V.parahaemolyticus colonies.The assays of avoiding interference demonstrated that,even in the presence of 2.1μg genomic DNA or 107 CFU background bacteria,V.parahaemolyticus could still be accurately detected.In addition,the IAC was used to indicate false-negative results,and lower than 94 copies of IAC per reaction had no influence on the detection limit.Ninety-six sea-food samples were tested,of which 58(60.4%)were positive,including 3 false negative results.Conse-quently,the real time PCR assay is effective for the rapid detection of V.parahaemotyticus contaminants in seafood.
基金financially supported by the Second Tibetan Plateau Scientific Expedition and Research Program(2019QZKK0606 and 2019QZKK0906)the National Key Research&Development Program of China(2016YFA0602400)。
文摘Every year,millions of people travel to high-elevation regions(≥2500 m above sea level)for sightseeing,mountaineering,trekking,skiing or commercial and scientific research activities.As the largest and highest plateau of the world,the Qinghai-Tibet Plateau(QTP)is one of the most attractive tourist destinations because of its multiple splendid sceneries.High-altitude illnesses occur when the rate of ascent to a high altitude overcomes the ability of the individual to acclimatize.Chronic mountain sickness(CMS)is one of the most important high-altitude pathologies in most high-altitude regions over the world.
基金supported by grants from National Natural Science Foundation of China(32000188)Fundamental Research Funds for the Central Universities(Grant lzujbky-2021-ey15 to Fang Lin,Grant lzujbky-2020-kb05 to Fang Lin,Grant lzujbky-2019-70 to Fang Lin)+2 种基金Science Fund for Distinguished Young Scholars of Gansu Province(22JR5RA393 to Fang Lin)the Young Elite Scientists Sponsorship Program by CAST(2019QNRC001 to Fang Lin)China postdoctoral Science Foundation.
文摘Light and phytohormone are external and internal cues that regulate plant growth and development throughout their life cycle.BBXs(B-box domain proteins)are a group of zinc finger proteins that not only directly govern the transcription of target genes but also associate with other factors to create a meticulous regulatory network to precisely regulate numerous aspects of growth and developmental processes in plants.Recent studies demonstrate that BBXs play pivotal roles in light-controlled plant growth and development.Besides,BBXs have been documented to regulate phytohormone-mediated physiological procedures.In this review,we summarize and highlight the multi-faced role of BBXs,with a focus in photomorphogenesis,photoperiodic flowering,shade avoidance,abiotic stress,and phytohormone-mediated growth and development in plant.
