The objective of this study was to characterize yellow （stripe） rust resistance gene（s） in 52 commercial wheat cultivars from Yunnan Province in China, and to provide information for their rational deployment in f...The objective of this study was to characterize yellow （stripe） rust resistance gene（s） in 52 commercial wheat cultivars from Yunnan Province in China, and to provide information for their rational deployment in field. Seedlings of wheat cultivars were inoculated with 25 differential isolates ofPuccinia striiformis from foreign and home to postulate resistance genes to yellow rust, and then validated by pedigree. There were 10 probable resistance genes characterized in these cultivars, in which, Yr9 was most commonly postulated to be present in thirteen cultivars. Yr21, the second, was present in four cultivars. Yr8, the third, were present in three cultivars. Yr6, Yrl 7 and Yr26, the fourth, was present in two cultivars respectively. The other gene（s） such as, Yr2＋YrA, Yr7 and Yr27, were only present in single cultivar（s）; unknown gene（s） or gene（s） combination（s） were present in 22 cultivars. One cultivar （Yunmai 42） had no resistance gene tested in this study. Cultivars such as Yunmai 52, Mian 1971-98, Kunmai 4, and Yunmai 56 carried effective genes and can be popularized mainly; Yr9 should be planted with other Yr genes. In the meantime other effective genes should be introduced to realize gene diversity for controlling wheat yellow rust. Yunmai 42 should be reduced to avoid rust breakout. Unknown gene cultivars should be utilized and be researched deeply.展开更多
A climate transition towards warm-wet conditions in Northwest China has drawn much attention.With continuous climate change and universal glacier degradation,increasing water-related hazards and vulnerability have bec...A climate transition towards warm-wet conditions in Northwest China has drawn much attention.With continuous climate change and universal glacier degradation,increasing water-related hazards and vulnerability have become one of the important problems facing the Tarim Basin.However,the impacts of the climate transition on streamflow abrupt change and extreme hydrological events were less discussed,especially in glacial basins.In the present study,the discharge datasets in four glacial basins of Tarim Basin from 1979 to 2018 were constructed using the GRU-GSWAT+model first.The differences in streamflow characteristics,the shift of hydrological extreme pattern,and potential changes of the controlling factors before and after the abrupt changes were investigated.The results indicated that the abrupt change point(ACP)in streamflow occurred in 2000 in the Qarqan River Basin,2002 in the Weigan River Basin,and 1994 in the Aksu River Basin and the Yarkant River Basin.A general decrease in streamflow before the ACP has shifted to a notable upward trend in the Qarqan River Basin and the Aksu River Basin,while minor upward fluctuations were observed in other basins.Moreover,the hydrological characteristics in extreme events vary dramatically before and after the ACPs,characterized by a pronouncing shift from drought-dominant pattern to wet events dominated pattern.The driven climate factors have been altered after the ACPs with notable spatial heterogeneity,in which temperature remained as the dominant role in meltwater-dominated basins while the influence of precipitation has increased after the ACPs,whereas the sensitivity of temperature on streamflow change has been enhanced in basins dominated by precipitation such as the Qarqan River Basin.Owing to the evident warming-wetting trend and glacier compensation effect,both the inter-annual and intra-annual streamflow fluctuations can be efficiently smoothed in basins with a high glacier area ratio(GAR).These findings provide a further understanding of the abrupt change in st展开更多
Stripe rust,caused by Puccinia striiformis f.sp.tritici（Pst）,is one of the most destructive diseases of wheat（Triticum aestivum L.）.To diversify stripe rust-resistant resources for wheat breeding programs,a CIMMYT...Stripe rust,caused by Puccinia striiformis f.sp.tritici（Pst）,is one of the most destructive diseases of wheat（Triticum aestivum L.）.To diversify stripe rust-resistant resources for wheat breeding programs,a CIMMYT synthetic wheat line CI110 was identified to be resistant to 28 isolates of Pst,including 6 Chinese prevalent races CYR28-CYR33.Genetic analysis indicated that a single dominant gene was responsible for the stripe rust resistance in CI110,temporarily designated YrC110.A molecular map,harboring YrC110 and 9 linked SSR markers,was constructed through simple sequence repeat（SSR）,and bulked segregant analysis.These linked markers and YrC110 were assigned on the short arm of chromosome 1B using the Chinese Spring nullisomic-tetrasomic and ditelosomic stocks.Gene postulation based on seedling reaction patterns to 30 Pst isolates suggested that the resistance gene YrC110 seemed different from the other known resistance genes tested,such as Yr9,Yr10,Yr15,Yr24,and Yr26/YrCH42.Four SSR markers Xbarc187150,Xgwm18227,Xgwm11223,and Xbarc240292 distinguished YrC110 from Yr10,Yr15,Yr24,and Yr26/YrCH42,and could be used as diagnostic ones for YrC110 in wheat resistant breeding programs against stripe rust.展开更多
The gene postulation, cluster analysis and pedigree analysis of 20 wheat cultivars to 28 physiological races of yellow rust （Puccinia striiformis） were conducted under greenhouse conditions, The spectrum of their re...The gene postulation, cluster analysis and pedigree analysis of 20 wheat cultivars to 28 physiological races of yellow rust （Puccinia striiformis） were conducted under greenhouse conditions, The spectrum of their resistance were compared each other. None of the 20 cultivars were resistant to all the test pathogens. The cultivars containing resistance genes Yr5 and Yr24 were not find and genes Yr8, Yrl9 and Yr27 could not be postulated from cultivars tested due to the susceptibility to all isolates used. A total of 6 probale seedling yellow rust resistance genes or gene combinations （Yrl, Yr2, Yr2 ＋ YrHVII, Yr3 ＋ unknown, Yr3 ＋ Yr4, YrAlba） were postulated in the wheat cultivars （Atou, Flanders, Maris Huntsman, Bouquet, Holdfast, Elite Lepeuple, and Vilmorin 27）. The gene combination Yr2＋YrHVII with the highest frequency （35%） was present in 7 cultivars. The Yr genes present in some wheat cultivars could not be postulated because of non-matching virulence combinations with any of known genes. Cluster result showed that Yr2 and Yr3 are the most important genes in the cultivars. The 13 cultivars are believed to have the pedigree of Noe, which was selected from South Russian wheat. These results will be useful for wheat breeding and provide information about genetic control of wheat yellow rust.展开更多
Background： Patellar dislocation is one of the most common knee injuries in the adolescent population. It is often combined with osteochondral fracture. The purpose of this study was to cornpare the outcomes between ...Background： Patellar dislocation is one of the most common knee injuries in the adolescent population. It is often combined with osteochondral fracture. The purpose of this study was to cornpare the outcomes between fixation and excision of osteochondral fractures not involving the bearing surface in adolescent patients with patellar dislocations. Methods： Patients who underwent surgery for osteochondral fracture following patellar dislocation in our institution from 2007 to 2014 were retrospectively evaluated. Visual analog scale （VAS） of pain and the International Knee Documentation Committee （IKI）C） form were used to assess knee pain and function at follow-up. Patient satisfaction was evaluated. Differences in the values of variables among groups were assessed using t-test if equal variance or Mann-Whitney U-test if not equal variance. The Pearson＇s Chi-square test was applied for dichotomous variables if expected frequency was 〉5 or Fisher＇s exact test was applied if not. A value of P 〈 0.05 was considered statistically significant. Results： Forty-three patients were included, with the average age of 14.1 ± 2.3 （range, 9.0-17.0） years. Nineteen underwent fixation of osteochondral fractures and 24 did not. The average follow-up time was 28 ± 10 months. There was no significant difference in age, gender, follow-up time, causes of injury, times of dislocation, and location of osteochondral fracture between fixation and excision groups. The fixation group had a significantly longer surgery time （82± 14 rain） and larger size of osteochondral fracture （2.30 ±0.70 cm2） than the excision group （43 ± 10 min, 1.88 ± 0.62 cm2, respectively, t = 10,77, P 〈 0.01 and t = 0.84, P 〈 0.05）. At the last follow-up, the average I KDC score in the fixation group （82.52 ± 8.71 ） was significantly lower than that in the excision group （89.51 ± 7.19, t 2.65, P 〈 0.01 ）. There was no significant difference in VAS of pain and patients＇ satisfaction. There were 7 （16%）展开更多
基金support by the Ministry of Science and Technology,China (2011CB100403)the Ministry of Agriculture,China (200903035)the Special Project from State Key Laboratory for Biology of Plant Diseases and Insect Pests,Chinese Academy of Agricltural Sciences (SKL2009OP09)
文摘The objective of this study was to characterize yellow （stripe） rust resistance gene（s） in 52 commercial wheat cultivars from Yunnan Province in China, and to provide information for their rational deployment in field. Seedlings of wheat cultivars were inoculated with 25 differential isolates ofPuccinia striiformis from foreign and home to postulate resistance genes to yellow rust, and then validated by pedigree. There were 10 probable resistance genes characterized in these cultivars, in which, Yr9 was most commonly postulated to be present in thirteen cultivars. Yr21, the second, was present in four cultivars. Yr8, the third, were present in three cultivars. Yr6, Yrl 7 and Yr26, the fourth, was present in two cultivars respectively. The other gene（s） such as, Yr2＋YrA, Yr7 and Yr27, were only present in single cultivar（s）; unknown gene（s） or gene（s） combination（s） were present in 22 cultivars. One cultivar （Yunmai 42） had no resistance gene tested in this study. Cultivars such as Yunmai 52, Mian 1971-98, Kunmai 4, and Yunmai 56 carried effective genes and can be popularized mainly; Yr9 should be planted with other Yr genes. In the meantime other effective genes should be introduced to realize gene diversity for controlling wheat yellow rust. Yunmai 42 should be reduced to avoid rust breakout. Unknown gene cultivars should be utilized and be researched deeply.
基金funded by the National Natural Science Foundation of China (41971094,41971083,42371145)the project of State Key Laboratory of Computer Science (SKLCS-ZZ-2023)+1 种基金the China-Pakistan joint program of the Chinese Academy of Sciences (046GJHZ2023069MI)the International Cooperation Program of CAS-CSIRO (131B62KYSB20190042).
文摘A climate transition towards warm-wet conditions in Northwest China has drawn much attention.With continuous climate change and universal glacier degradation,increasing water-related hazards and vulnerability have become one of the important problems facing the Tarim Basin.However,the impacts of the climate transition on streamflow abrupt change and extreme hydrological events were less discussed,especially in glacial basins.In the present study,the discharge datasets in four glacial basins of Tarim Basin from 1979 to 2018 were constructed using the GRU-GSWAT+model first.The differences in streamflow characteristics,the shift of hydrological extreme pattern,and potential changes of the controlling factors before and after the abrupt changes were investigated.The results indicated that the abrupt change point(ACP)in streamflow occurred in 2000 in the Qarqan River Basin,2002 in the Weigan River Basin,and 1994 in the Aksu River Basin and the Yarkant River Basin.A general decrease in streamflow before the ACP has shifted to a notable upward trend in the Qarqan River Basin and the Aksu River Basin,while minor upward fluctuations were observed in other basins.Moreover,the hydrological characteristics in extreme events vary dramatically before and after the ACPs,characterized by a pronouncing shift from drought-dominant pattern to wet events dominated pattern.The driven climate factors have been altered after the ACPs with notable spatial heterogeneity,in which temperature remained as the dominant role in meltwater-dominated basins while the influence of precipitation has increased after the ACPs,whereas the sensitivity of temperature on streamflow change has been enhanced in basins dominated by precipitation such as the Qarqan River Basin.Owing to the evident warming-wetting trend and glacier compensation effect,both the inter-annual and intra-annual streamflow fluctuations can be efficiently smoothed in basins with a high glacier area ratio(GAR).These findings provide a further understanding of the abrupt change in st
文摘Stripe rust,caused by Puccinia striiformis f.sp.tritici（Pst）,is one of the most destructive diseases of wheat（Triticum aestivum L.）.To diversify stripe rust-resistant resources for wheat breeding programs,a CIMMYT synthetic wheat line CI110 was identified to be resistant to 28 isolates of Pst,including 6 Chinese prevalent races CYR28-CYR33.Genetic analysis indicated that a single dominant gene was responsible for the stripe rust resistance in CI110,temporarily designated YrC110.A molecular map,harboring YrC110 and 9 linked SSR markers,was constructed through simple sequence repeat（SSR）,and bulked segregant analysis.These linked markers and YrC110 were assigned on the short arm of chromosome 1B using the Chinese Spring nullisomic-tetrasomic and ditelosomic stocks.Gene postulation based on seedling reaction patterns to 30 Pst isolates suggested that the resistance gene YrC110 seemed different from the other known resistance genes tested,such as Yr9,Yr10,Yr15,Yr24,and Yr26/YrCH42.Four SSR markers Xbarc187150,Xgwm18227,Xgwm11223,and Xbarc240292 distinguished YrC110 from Yr10,Yr15,Yr24,and Yr26/YrCH42,and could be used as diagnostic ones for YrC110 in wheat resistant breeding programs against stripe rust.
基金supported by the project from the National Basic Research Program of China (2006CB100200)China Postdoctoral Science Foundation (20080430052)+1 种基金the National Natural Science Foundation of China(30471131)the National Key Technology R&D Program of China(2006BAD08A05,2006BAD02A16)
文摘The gene postulation, cluster analysis and pedigree analysis of 20 wheat cultivars to 28 physiological races of yellow rust （Puccinia striiformis） were conducted under greenhouse conditions, The spectrum of their resistance were compared each other. None of the 20 cultivars were resistant to all the test pathogens. The cultivars containing resistance genes Yr5 and Yr24 were not find and genes Yr8, Yrl9 and Yr27 could not be postulated from cultivars tested due to the susceptibility to all isolates used. A total of 6 probale seedling yellow rust resistance genes or gene combinations （Yrl, Yr2, Yr2 ＋ YrHVII, Yr3 ＋ unknown, Yr3 ＋ Yr4, YrAlba） were postulated in the wheat cultivars （Atou, Flanders, Maris Huntsman, Bouquet, Holdfast, Elite Lepeuple, and Vilmorin 27）. The gene combination Yr2＋YrHVII with the highest frequency （35%） was present in 7 cultivars. The Yr genes present in some wheat cultivars could not be postulated because of non-matching virulence combinations with any of known genes. Cluster result showed that Yr2 and Yr3 are the most important genes in the cultivars. The 13 cultivars are believed to have the pedigree of Noe, which was selected from South Russian wheat. These results will be useful for wheat breeding and provide information about genetic control of wheat yellow rust.
基金This work was supported by grants from the General Financial Grant from the China Postdoctoral Science Foundation （No. 2016M602846）, the National Natural Science Foundation of China （No. 81702210）, and the Shaanxi Postdoctoral Science Foundation （No. 2017BSHQYXMZZ 16）.
文摘Background： Patellar dislocation is one of the most common knee injuries in the adolescent population. It is often combined with osteochondral fracture. The purpose of this study was to cornpare the outcomes between fixation and excision of osteochondral fractures not involving the bearing surface in adolescent patients with patellar dislocations. Methods： Patients who underwent surgery for osteochondral fracture following patellar dislocation in our institution from 2007 to 2014 were retrospectively evaluated. Visual analog scale （VAS） of pain and the International Knee Documentation Committee （IKI）C） form were used to assess knee pain and function at follow-up. Patient satisfaction was evaluated. Differences in the values of variables among groups were assessed using t-test if equal variance or Mann-Whitney U-test if not equal variance. The Pearson＇s Chi-square test was applied for dichotomous variables if expected frequency was 〉5 or Fisher＇s exact test was applied if not. A value of P 〈 0.05 was considered statistically significant. Results： Forty-three patients were included, with the average age of 14.1 ± 2.3 （range, 9.0-17.0） years. Nineteen underwent fixation of osteochondral fractures and 24 did not. The average follow-up time was 28 ± 10 months. There was no significant difference in age, gender, follow-up time, causes of injury, times of dislocation, and location of osteochondral fracture between fixation and excision groups. The fixation group had a significantly longer surgery time （82± 14 rain） and larger size of osteochondral fracture （2.30 ±0.70 cm2） than the excision group （43 ± 10 min, 1.88 ± 0.62 cm2, respectively, t = 10,77, P 〈 0.01 and t = 0.84, P 〈 0.05）. At the last follow-up, the average I KDC score in the fixation group （82.52 ± 8.71 ） was significantly lower than that in the excision group （89.51 ± 7.19, t 2.65, P 〈 0.01 ）. There was no significant difference in VAS of pain and patients＇ satisfaction. There were 7 （16%）
