The hippocampus is one of the most commonly studied brain regions in the context of depression.The volume of the hippocampus is significantly reduced in patients with depression,which severely disrupts hippocampal neu...The hippocampus is one of the most commonly studied brain regions in the context of depression.The volume of the hippocampus is significantly reduced in patients with depression,which severely disrupts hippocampal neuroplasticity.However,antidepressant therapies that target hippocampal neuroplasticity have not been identified as yet.Chinese medicine(CM)can slow the progression of depression,potentially by modulating hippocampal neuroplasticity.Xiaoyaosan(XYS)is a CM formula that has been clinically used for the treatment of depression.It is known to protect Gan(Liver)and Pi(Spleen)function,and may exert its antidepressant effects by regulating hippocampal neuroplasticity.In this review,we have summarized the association between depression and aberrant hippocampal neuroplasticity.Furthermore,we have discussed the researches published in the last 30 years on the effects of XYS on hippocampal neuroplasticity in order to elucidate the possible mechanisms underlying its therapeutic action against depression.The results of this review can aid future research on XYS for the treatment of depression.展开更多
Objective: As a traditional medicinal plant listed in the Chinese Pharmacopeia, Physalis alkekengi L. var. franchetii(Mast.) Makino(PAF) has a long medicinal history and high economic value. PAF has immunomodulatory p...Objective: As a traditional medicinal plant listed in the Chinese Pharmacopeia, Physalis alkekengi L. var. franchetii(Mast.) Makino(PAF) has a long medicinal history and high economic value. PAF has immunomodulatory properties and can be used to treat acute lung injury and eczema.The aim of this study is to solve the problems of extraction and purification of active components from PAF. Materials and Methods: The solvent to be used for extraction and its concentration, the solid-to-liquid ratio, and extraction duration were investigated using a single-factor experiment.An orthogonal design(L_(9)[3^(4)]) was used to determine the optimum extraction conditions. After optimization, the sample’s concentrations and flow velocity, the eluents and their velocity, adsorption time, and the removed water volume were measured. The content of the five steroids in the sample was determined by high-performance liquid chromatography(HPLC). We also investigated the anti-inflammatory property of PAF calyxes before and after purification. Results: The optimum extraction and purification processes were determined by single-factor analysis.AB-8 was identified as the best macroporous adsorption resin for enrichment. After optimization, the average total steroid content was 71.83%,and the average recovery was 90% after purification. Among the five steroid components detected by HPLC, physalin F showed the highest content. Furthermore, the sample obtained after purification could significantly inhibit paw edema by egg whites induced. Conclusions: An environmentally-sustainable, efficient, and stable process was first optimized for enriching and purifying total steroids from PAF. The process has the potential for further development and utilization in the pharmaceutical industry.展开更多
Objective: To assess the short-term effect of scaling and root planing (SRP) and essential-oils mouthwash on the levels of specific bacteria in Chinese adults. Methods: Fifty Chinese adults with chronic periodontitis ...Objective: To assess the short-term effect of scaling and root planing (SRP) and essential-oils mouthwash on the levels of specific bacteria in Chinese adults. Methods: Fifty Chinese adults with chronic periodontitis were randomly assigned to full-mouth SRP or a 7-d essential-oils mouthwash regimen. In addition, 22 periodontally healthy adults used essential-oils mouthwash for 7 d. Clinical examination and plaque/saliva sampling were performed at baseline and on Day 7. Quantitative real-time polymerase chain reaction (PCR) was used to measure Aggregatibacter actinomycetemcomitans (Aa), Fusobacterium nucleatum (Fn), Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), and total bacterial loads in saliva, supra- and sub-gingival plaque samples. Results: The detection frequencies of four tested species remained unchanged after either treatment. However, the bacterial loads of Fn, Pg, and Pi were significantly reduced by SRP; the mean reduction of bacterial counts in saliva ranged from 52.2% to 62.5% (p<0.01), in supragingival plaque from 68.2% to 81.0% (p<0.05), and in subgingival plaque from 67.9% to 93.0% (p<0.01). Total bacterial loads were reduced after SRP in supra- and sub-gingival plaque (p<0.05). Essential-oils mouthwash reduced Fn levels in supragingival plaque by a mean of 53.2%, and reduced total bacterial loads in supra- and sub-gingival plaque (p<0.01). In subgingival plaque from periodontal patients, Pg and Pi reductions were high after SRP compared to essential-oils mouthwash (93.0% vs. 37.7% and 87.0% vs. 21.0%, p<0.05). No significant bacterial reduction was observed in periodontally healthy subjects using essential-oils mouthwash. Conclusions: SRP and essential-oils mouthwash both have an impact on saliva and gingival plaque flora in Chinese periodontitis patients in 7 d, with greater microbiological improvement by SRP.展开更多
【目的】研究不同栽培方式下不同播种深度对马铃薯土壤水热及产量的影响,为冬种马铃薯栽培技术提供重要参考。【方法】以费乌瑞它、丽薯6号为试验材料,设置黑膜覆盖、稻草覆盖和常规种植3种栽培方式下的5、10、15和20 cm 4种不同播种深...【目的】研究不同栽培方式下不同播种深度对马铃薯土壤水热及产量的影响,为冬种马铃薯栽培技术提供重要参考。【方法】以费乌瑞它、丽薯6号为试验材料,设置黑膜覆盖、稻草覆盖和常规种植3种栽培方式下的5、10、15和20 cm 4种不同播种深度处理,测定马铃薯土壤含水量、温度、物候期及产量等农艺性状。【结果】与常规种植相比,丽薯6号黑膜覆盖和稻草覆盖2种栽培方式下的土壤温度均提高1.9℃,在苗期、发棵期、结薯期,黑膜覆盖5~10 cm土层的土壤含水量均最大;在黑膜覆盖播种深度5和10 cm处理下,2个马铃薯品种的生育期最短;丽薯6号和费乌瑞它分别在黑膜覆盖播种深度为10和15 cm时,各农艺性状、产量(57.20、36.30 t/hm2)和经济效益表现最优。【结论】广西冬种马铃薯丽薯6号、费乌瑞它2个品种的最佳栽培方式分别为黑膜覆盖播种深度10和15 cm,研究结果可为广西冬种马铃薯生产合理选择和应用栽培方式提供科学参考。展开更多
目的：系统评价中医辨证论治多发性硬化（MS）的临床疗效。方法：全面检索中国期刊全文数据库、维普中文科技期刊全文数据库、中国生物医学文献服务系统、万方医药期刊数据库、Pub Med、Cochrane图书馆等数据库。中文检索词以＂多发性...目的：系统评价中医辨证论治多发性硬化（MS）的临床疗效。方法：全面检索中国期刊全文数据库、维普中文科技期刊全文数据库、中国生物医学文献服务系统、万方医药期刊数据库、Pub Med、Cochrane图书馆等数据库。中文检索词以＂多发性硬化＂和＂中药或针灸或中西医结合＂为并列检索词,英文检索词为＂multiple sclerosis or MS＂AND＂traditional Chinese medicine or herb or herbal or acupuncture or combined traditional Chinese and western medicine＂检索中医辨证治疗MS的随机对照临床试验文献,时间区间设定为2000年1月1日—2016年10月30日。按照Cochrane系统评价手册推荐的简单评价法,评价纳入研究的方法学质量。软件版本为Review Manger 5.3,结局效应指标分别为EDSS评分（EDSS）、有效率、复发次数、临床症状评分、神经功能体征评分。结果：最终纳入16个随机对照临床试验,共906例研究对象。所纳入的研究中有13项研究报道了EDSS评分,纳入统计的为11项,试验组与对照组比较,MD及95%CI为-0.96（-1.50,-0.41）,P〈0.05,有统计学差异;有12项研究观察了有效率,11项研究可提取有效数据,OR值为4.93,95%CI为（3.05,7.96）,P〈0.001,统计学有显著差异;有3项研究对患者进行了复发次数评价,MD及95%CI为-0.43（-0.67,-0.19）,P〈0.05,有统计学差异;有8项研究观察了临床症状评分,7项可提取有效数据,MD及95%CI为-1.09（-3.41,1.23）,P〉0.05,统计学无差异;有4项研究观察了神经功能体征评分,试验组与对照组比较,MD及95%CI为-1.86（-4.70,0.97）,P〉0.05,统计学无差异。结论：中医辨证论治结合西药治疗MS相对于单纯西药治疗,提高了其有效率,减少MS的复发次数,改善神经功能缺损。展开更多
基金Supported by the National Natural Science Foundation of China(No.82304746)Jinan University Innovation and Entrepreneurship Training Program for Undergraduate(No.CX21255 and CX22481)。
文摘The hippocampus is one of the most commonly studied brain regions in the context of depression.The volume of the hippocampus is significantly reduced in patients with depression,which severely disrupts hippocampal neuroplasticity.However,antidepressant therapies that target hippocampal neuroplasticity have not been identified as yet.Chinese medicine(CM)can slow the progression of depression,potentially by modulating hippocampal neuroplasticity.Xiaoyaosan(XYS)is a CM formula that has been clinically used for the treatment of depression.It is known to protect Gan(Liver)and Pi(Spleen)function,and may exert its antidepressant effects by regulating hippocampal neuroplasticity.In this review,we have summarized the association between depression and aberrant hippocampal neuroplasticity.Furthermore,we have discussed the researches published in the last 30 years on the effects of XYS on hippocampal neuroplasticity in order to elucidate the possible mechanisms underlying its therapeutic action against depression.The results of this review can aid future research on XYS for the treatment of depression.
基金supported by the Guangzhou Science and Technology Program in 2020-Basic and Applied Basic Research Project(Grant No.202002030226)the National Natural Science Foundation of China(Grant No.82174043)the Guangdong Basic and Applied Basic Research Foundation(Grant No.2021A1515011697).
文摘Objective: As a traditional medicinal plant listed in the Chinese Pharmacopeia, Physalis alkekengi L. var. franchetii(Mast.) Makino(PAF) has a long medicinal history and high economic value. PAF has immunomodulatory properties and can be used to treat acute lung injury and eczema.The aim of this study is to solve the problems of extraction and purification of active components from PAF. Materials and Methods: The solvent to be used for extraction and its concentration, the solid-to-liquid ratio, and extraction duration were investigated using a single-factor experiment.An orthogonal design(L_(9)[3^(4)]) was used to determine the optimum extraction conditions. After optimization, the sample’s concentrations and flow velocity, the eluents and their velocity, adsorption time, and the removed water volume were measured. The content of the five steroids in the sample was determined by high-performance liquid chromatography(HPLC). We also investigated the anti-inflammatory property of PAF calyxes before and after purification. Results: The optimum extraction and purification processes were determined by single-factor analysis.AB-8 was identified as the best macroporous adsorption resin for enrichment. After optimization, the average total steroid content was 71.83%,and the average recovery was 90% after purification. Among the five steroid components detected by HPLC, physalin F showed the highest content. Furthermore, the sample obtained after purification could significantly inhibit paw edema by egg whites induced. Conclusions: An environmentally-sustainable, efficient, and stable process was first optimized for enriching and purifying total steroids from PAF. The process has the potential for further development and utilization in the pharmaceutical industry.
基金Project supported by the Zhejiang Provincial Natural Science Foundation of China (No. Y2080253)the Education Department of Zhejiang Province (No. Y200909376)+1 种基金the Science and Technology Department of Zhejiang Province (No. 2007C24010)2011 China State Key Clinical Department Grants
文摘Objective: To assess the short-term effect of scaling and root planing (SRP) and essential-oils mouthwash on the levels of specific bacteria in Chinese adults. Methods: Fifty Chinese adults with chronic periodontitis were randomly assigned to full-mouth SRP or a 7-d essential-oils mouthwash regimen. In addition, 22 periodontally healthy adults used essential-oils mouthwash for 7 d. Clinical examination and plaque/saliva sampling were performed at baseline and on Day 7. Quantitative real-time polymerase chain reaction (PCR) was used to measure Aggregatibacter actinomycetemcomitans (Aa), Fusobacterium nucleatum (Fn), Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), and total bacterial loads in saliva, supra- and sub-gingival plaque samples. Results: The detection frequencies of four tested species remained unchanged after either treatment. However, the bacterial loads of Fn, Pg, and Pi were significantly reduced by SRP; the mean reduction of bacterial counts in saliva ranged from 52.2% to 62.5% (p<0.01), in supragingival plaque from 68.2% to 81.0% (p<0.05), and in subgingival plaque from 67.9% to 93.0% (p<0.01). Total bacterial loads were reduced after SRP in supra- and sub-gingival plaque (p<0.05). Essential-oils mouthwash reduced Fn levels in supragingival plaque by a mean of 53.2%, and reduced total bacterial loads in supra- and sub-gingival plaque (p<0.01). In subgingival plaque from periodontal patients, Pg and Pi reductions were high after SRP compared to essential-oils mouthwash (93.0% vs. 37.7% and 87.0% vs. 21.0%, p<0.05). No significant bacterial reduction was observed in periodontally healthy subjects using essential-oils mouthwash. Conclusions: SRP and essential-oils mouthwash both have an impact on saliva and gingival plaque flora in Chinese periodontitis patients in 7 d, with greater microbiological improvement by SRP.
文摘目的：系统评价中医辨证论治多发性硬化（MS）的临床疗效。方法：全面检索中国期刊全文数据库、维普中文科技期刊全文数据库、中国生物医学文献服务系统、万方医药期刊数据库、Pub Med、Cochrane图书馆等数据库。中文检索词以＂多发性硬化＂和＂中药或针灸或中西医结合＂为并列检索词,英文检索词为＂multiple sclerosis or MS＂AND＂traditional Chinese medicine or herb or herbal or acupuncture or combined traditional Chinese and western medicine＂检索中医辨证治疗MS的随机对照临床试验文献,时间区间设定为2000年1月1日—2016年10月30日。按照Cochrane系统评价手册推荐的简单评价法,评价纳入研究的方法学质量。软件版本为Review Manger 5.3,结局效应指标分别为EDSS评分（EDSS）、有效率、复发次数、临床症状评分、神经功能体征评分。结果：最终纳入16个随机对照临床试验,共906例研究对象。所纳入的研究中有13项研究报道了EDSS评分,纳入统计的为11项,试验组与对照组比较,MD及95%CI为-0.96（-1.50,-0.41）,P〈0.05,有统计学差异;有12项研究观察了有效率,11项研究可提取有效数据,OR值为4.93,95%CI为（3.05,7.96）,P〈0.001,统计学有显著差异;有3项研究对患者进行了复发次数评价,MD及95%CI为-0.43（-0.67,-0.19）,P〈0.05,有统计学差异;有8项研究观察了临床症状评分,7项可提取有效数据,MD及95%CI为-1.09（-3.41,1.23）,P〉0.05,统计学无差异;有4项研究观察了神经功能体征评分,试验组与对照组比较,MD及95%CI为-1.86（-4.70,0.97）,P〉0.05,统计学无差异。结论：中医辨证论治结合西药治疗MS相对于单纯西药治疗,提高了其有效率,减少MS的复发次数,改善神经功能缺损。
