Rice production accounts for approximately half of the freshwater resources utilized in agriculture,result-ing in greenhouse gas emissions such as methane(CH4)from flooded paddy fields.To address this chal-lenge,envir...Rice production accounts for approximately half of the freshwater resources utilized in agriculture,result-ing in greenhouse gas emissions such as methane(CH4)from flooded paddy fields.To address this chal-lenge,environmentally friendly and cost-effective water-saving techniques have become widely adopted in rice cultivation.However,the implementation of water-saving treatments(WsTs)in paddy-field rice has been associated with a substantial yield loss of up to 50%as well as a reduction in nitrogen use efficiency(NUE).In this study,we discovered that the target of rapamycin(TOR)signaling pathway is compromised in rice under WsT.Polysome profiling-coupled transcriptome sequencing(polysome-seq)analysis unveiled a substantial reduction in global translation in response to WST associated with the downregulation of TOR activity.Molecular,biochemical,and genetic analyses revealed new insights into the impact of the positive TOR-S6K-RPS6 and negative TOR-MAF1 modules on translation repression under WST.Intriguingly,ammonium exhibited a greater ability to alleviate growth constraints under WsT by enhancing TOR signaling,which simultaneously promoted uptake and utilization of ammonium and nitrogen allocation.We further demonstrated that TOR modulates the ammonium transporter AMT1;1 as well as the amino acid permease APP1 and dipeptide transporter NPF7.3 at the translational level through the 5'untranslated region.Collectively,these findings reveal that enhancing TOR signaling could mitigate rice yield penalty due to WST by regulating the processes involved in protein synthesis and NUE.Our study will contribute to the breeding of new rice varieties with increased water and fertilizer utilization efficiency.展开更多
Dear Editor,Gastric cancer(GC)is among the most prevalent gastrointestinal malignancies.The occurrence of local deep infiltration or distant metastasis in GC is commonly associated with weak treatment and poor prognos...Dear Editor,Gastric cancer(GC)is among the most prevalent gastrointestinal malignancies.The occurrence of local deep infiltration or distant metastasis in GC is commonly associated with weak treatment and poor prognosis.1 Although,N4-Acetylcytidine(ac4C)represents one of the extensive chemical modifications in mRNAs that plays a pivotal role in modulating mRNA stability and the mRNA translation process(Fig.1b).展开更多
hotodynamic therapy(PDT)has been drawing more and more attention in the antibacterial field.Traditional photosensitizers(PSs)tend to aggregate in aqueous media,which reduces the generation of reactive oxygen species(R...hotodynamic therapy(PDT)has been drawing more and more attention in the antibacterial field.Traditional photosensitizers(PSs)tend to aggregate in aqueous media,which reduces the generation of reactive oxygen species(ROS)and seriously affects the photodynamic efficacy.Many efforts have been made to prevent aggregation of traditional PSs.By contrast,aggregation-induced emission PSs(AIE-PSs)take advantage of aggregation to boost ROS generation and fluorescence intensity.However,the efficacies of the reported antibacterial AIE-PSs are poor.Herein,we report a new class of highly effective antibacterial AIE-PSs based on nitrobenzoic acid structure.TTVBA,a negatively charged AIE-PS,can not only selectively kill spherical bacteria(Staphylococcus aureus(S.aureus))rather than rod-shaped bacteria(Escherichia coli(E.coli)),but also be easily extended to several AIE-PSs(TTVBP1–3)with positive charges and broad-spectrum antibacterial activity.We demonstrate that TTVBP2 can kill3.0 log_(10)of S.aureus at very low concentration(125 nmol L^(-1)),TTVBP3 can kill 4.7 log_(10)of Staphylococcus epidermidis(S.epidermidis)at a concentration of 1μmol L^(-1)and 3.8 log_(10)of E.coli at 5μmol L^(-1),thus enabling them among the most effective antibacterial AIE-PSs reported so far.Meanwhile,these AIE-PSs exhibit excellent wash-free imaging ability for bacteria by simple mixing with bacteria.We thus envision that TTVBA,a nitrobenzoic acid-based extendable AIE-PS,provides a new route for the design of AIE-PSs in antibacterial treatment.展开更多
Fast skin repair is critical for less infection, less pain and high quality of life, which is still limited with undesirable rehabilitation speed and side effects. Currently, laser-activated silk sealant agent without...Fast skin repair is critical for less infection, less pain and high quality of life, which is still limited with undesirable rehabilitation speed and side effects. Currently, laser-activated silk sealant agent without suture and gauze has been demonstrated promising for fast skin repair taking advantage of its structural transformation after heating. Nevertheless, more efficient healing effects and less side effects of laseractivated silk sealant agent remains challenging due to absence of suitable photo-thermal materials and robust/biomimetic protein materials. In this work, the marriage between silk protein and Rehmanniae radix preparata(a kind of the traditional Chinese herb) has been demonstrated as a novel and effective way to achieve an excellent healing effect for skin repair. The non-toxicity, high photothermal conversion efficiency and healing mechanism are systematically studied and proved. This new methodology might shed a new light for combining dark traditional Chinese medicine and silk fibroin for advanced wound healing technology.展开更多
基金Thise research was supported by the Hainan Provincial Joint Project of Sanya Yazhou Bay Science and Technology City City(320LH031 and HSPHDSRF-2023-04-016)Zhejiang Provincial Natural Science Foundation of China(LY21C020003)+3 种基金Zhejiang University Global Partnership Fund,Fundamental Research Funds for the Central Universities for the Central Universities(K20200168)the Key Research and Development Program of Zhejiang(2020C02002)National Natural Science Foundation of China(32201819)China Postdoctoral Science Foundation(2022M712807).
文摘Rice production accounts for approximately half of the freshwater resources utilized in agriculture,result-ing in greenhouse gas emissions such as methane(CH4)from flooded paddy fields.To address this chal-lenge,environmentally friendly and cost-effective water-saving techniques have become widely adopted in rice cultivation.However,the implementation of water-saving treatments(WsTs)in paddy-field rice has been associated with a substantial yield loss of up to 50%as well as a reduction in nitrogen use efficiency(NUE).In this study,we discovered that the target of rapamycin(TOR)signaling pathway is compromised in rice under WsT.Polysome profiling-coupled transcriptome sequencing(polysome-seq)analysis unveiled a substantial reduction in global translation in response to WST associated with the downregulation of TOR activity.Molecular,biochemical,and genetic analyses revealed new insights into the impact of the positive TOR-S6K-RPS6 and negative TOR-MAF1 modules on translation repression under WST.Intriguingly,ammonium exhibited a greater ability to alleviate growth constraints under WsT by enhancing TOR signaling,which simultaneously promoted uptake and utilization of ammonium and nitrogen allocation.We further demonstrated that TOR modulates the ammonium transporter AMT1;1 as well as the amino acid permease APP1 and dipeptide transporter NPF7.3 at the translational level through the 5'untranslated region.Collectively,these findings reveal that enhancing TOR signaling could mitigate rice yield penalty due to WST by regulating the processes involved in protein synthesis and NUE.Our study will contribute to the breeding of new rice varieties with increased water and fertilizer utilization efficiency.
基金the National Natural Science Foundation of China(Grant no.81960273)Gansu Natural Science Foundation(No.18JR3RA343)+4 种基金Gansu Fund project for Distinguished Young Scholars(No.18JR3RA262)the Key Projects of Department of Science and Technology in Gansu Province,China(No.1602FKDA001)the Science and Technology Innovation and Development Special Funding of Gansu province,China(G.F.R[2018]No.32)the Science and Technology Bureau 2018 Fund of the Chengguan District(2018KJGG0037)National Key Research and Development Program of China(No.2017FYA0205302).
文摘Dear Editor,Gastric cancer(GC)is among the most prevalent gastrointestinal malignancies.The occurrence of local deep infiltration or distant metastasis in GC is commonly associated with weak treatment and poor prognosis.1 Although,N4-Acetylcytidine(ac4C)represents one of the extensive chemical modifications in mRNAs that plays a pivotal role in modulating mRNA stability and the mRNA translation process(Fig.1b).
基金supported by the National Natural Science Foundation of China (81572944 and 81971983)the CAS/ SAFEA International Partnership Program for Creative Research Teams+1 种基金the High-Level Entrepreneurship and Innovation Talents Projects in Fujian Province (2018-8-1)the FJIRSM&IUE Joint Research Fund (RHZX-2018-004)
文摘hotodynamic therapy(PDT)has been drawing more and more attention in the antibacterial field.Traditional photosensitizers(PSs)tend to aggregate in aqueous media,which reduces the generation of reactive oxygen species(ROS)and seriously affects the photodynamic efficacy.Many efforts have been made to prevent aggregation of traditional PSs.By contrast,aggregation-induced emission PSs(AIE-PSs)take advantage of aggregation to boost ROS generation and fluorescence intensity.However,the efficacies of the reported antibacterial AIE-PSs are poor.Herein,we report a new class of highly effective antibacterial AIE-PSs based on nitrobenzoic acid structure.TTVBA,a negatively charged AIE-PS,can not only selectively kill spherical bacteria(Staphylococcus aureus(S.aureus))rather than rod-shaped bacteria(Escherichia coli(E.coli)),but also be easily extended to several AIE-PSs(TTVBP1–3)with positive charges and broad-spectrum antibacterial activity.We demonstrate that TTVBP2 can kill3.0 log_(10)of S.aureus at very low concentration(125 nmol L^(-1)),TTVBP3 can kill 4.7 log_(10)of Staphylococcus epidermidis(S.epidermidis)at a concentration of 1μmol L^(-1)and 3.8 log_(10)of E.coli at 5μmol L^(-1),thus enabling them among the most effective antibacterial AIE-PSs reported so far.Meanwhile,these AIE-PSs exhibit excellent wash-free imaging ability for bacteria by simple mixing with bacteria.We thus envision that TTVBA,a nitrobenzoic acid-based extendable AIE-PS,provides a new route for the design of AIE-PSs in antibacterial treatment.
基金supported by the National Natural Science Foundation of China (Nos. 81972488 and 81973013)the Eighth Affiliated Hospital of Sun Yat-sen University Outstanding Youth Reserve Talent Science Fund (No. FBJQ2019002)+2 种基金the Guangdong Key R&D Program (No. 2019B020210002)the Guangdong Natural Science Foundation (No. C1051164)China Postdoctoral Science Foundation(Nos. 2019TQ0242, 2019M660061XB)。
文摘Fast skin repair is critical for less infection, less pain and high quality of life, which is still limited with undesirable rehabilitation speed and side effects. Currently, laser-activated silk sealant agent without suture and gauze has been demonstrated promising for fast skin repair taking advantage of its structural transformation after heating. Nevertheless, more efficient healing effects and less side effects of laseractivated silk sealant agent remains challenging due to absence of suitable photo-thermal materials and robust/biomimetic protein materials. In this work, the marriage between silk protein and Rehmanniae radix preparata(a kind of the traditional Chinese herb) has been demonstrated as a novel and effective way to achieve an excellent healing effect for skin repair. The non-toxicity, high photothermal conversion efficiency and healing mechanism are systematically studied and proved. This new methodology might shed a new light for combining dark traditional Chinese medicine and silk fibroin for advanced wound healing technology.
