Rydberg atoms-based electric field sensing has developed rapidly over the past decade.A variety of theoretical proposals and experiment configurations are suggested and realized to improve the measurement metrics,such...Rydberg atoms-based electric field sensing has developed rapidly over the past decade.A variety of theoretical proposals and experiment configurations are suggested and realized to improve the measurement metrics,such as intensity sensitivity,bandwidth,phase,and accuracy.The Stark effect and electromagnetically induced transparency(EIT)or electromagnetically induced absorption(EIA)are fundamental physics principles behind the stage.Furthermore,various techniques such as amplitude-or frequency-modulation,optical homodyne read-out,microwave superheterodyne and frequency conversion based on multi-wave mixing in atoms are utilized to push the metrics into higher levels.In this review,different technologies and the corresponding metrics they had achieved were presented,hoping to inspire more possibilities in the improvement of metrics of Rydberg atom-based electric field sensing and broadness of application scenarios.展开更多
构建随机ssDNA文库,通过SELEX技术,以正常、炎性宫颈脱落细胞为反筛细胞,以上皮内低级别病变(CIN1)、上皮内高级别病变(CIN2、CIN3)和鳞状细胞癌脱落细胞为正筛细胞,经过12轮筛选特异性适配子高度富集得到宫颈癌前病变适配子库,经特异...构建随机ssDNA文库,通过SELEX技术,以正常、炎性宫颈脱落细胞为反筛细胞,以上皮内低级别病变(CIN1)、上皮内高级别病变(CIN2、CIN3)和鳞状细胞癌脱落细胞为正筛细胞,经过12轮筛选特异性适配子高度富集得到宫颈癌前病变适配子库,经特异性、亲和力分析和细胞免疫荧光确立高特异性适配子CIN-Ap4可作为诊断宫颈癌前病变生物标志物,为宫颈癌前病变分子诊断奠定理论基础,提供新思路。利用Prime Premier 5.0设计构建了随机ssDNA文库并根据文库两端固定序列设计引物,对对称PCR和间接不对称PCR中的退火温度、循环数以及上、下游引物浓度比等条件进行优化,分析确定50μL反应体系中对称PCR的最佳反应条件为:最佳退火温度为49.5℃,最佳循环数为15个循环;间接不对称PCR的最佳反应条件为:50μL反应体系中上、下游引物浓度的最佳比例为80∶1,最佳循环数为35个循环。实验结果表明成功构建了寡核苷酸文库,在最适PCR条件下可获得理想的dsDNA和ssDNA,并具有良好的重复性,为顺利筛选适配子提供保证。展开更多
结直肠癌是世界高发和高致死率的恶性肿瘤。靶向新抗原的免疫治疗已被证实可以诱导癌症患者肿瘤持续消退,但这些特异性新抗原,仅适用于个体精准治疗。随着大量的高频肿瘤基因突变被发现,这些与突变相关的高频新抗原可覆盖更多人群,具有...结直肠癌是世界高发和高致死率的恶性肿瘤。靶向新抗原的免疫治疗已被证实可以诱导癌症患者肿瘤持续消退,但这些特异性新抗原,仅适用于个体精准治疗。随着大量的高频肿瘤基因突变被发现,这些与突变相关的高频新抗原可覆盖更多人群,具有较强的临床意义。然而目前结直肠癌中是否也存在高频新抗原仍不清楚。本研究利用来源于321个结直肠癌患者的体细胞突变数据库,联合1种标准过滤和7种预测算法,筛选并获得了25个基于中国人高频分型HLA-A^*1101限制性的高频新抗原,它们均具有高亲和力(IC50<50 nmol/L)和高呈递分值(>0.90);其中,除了阳性对照多肽KRAS_G12V8-16外,11个高频新抗原能够在体外诱导细胞毒性T淋巴细胞(cytotoxic T lymphocyte,CTL)分泌γ干扰素(interferon gamma,IFN-γ),证实具有免疫原性。选取免疫原性最强的新抗原C1orf170_S418G413-421及阳性对照多肽KRAS_G12V8-16体外刺激T细胞,利用流式细胞分选及单细胞转录组测序技术,获得其特异性CTL的免疫组库信息,所构建的TCR-T(T-cell receptor engineered T cell)能够识别新抗原并分泌细胞因子。以上结果表明,本研究开发了一种利用体细胞数据库预测并体外筛选验证具有免疫原性高频新抗原的方法,为结直肠癌及其他癌种的多肽、DC(dendritic cells)疫苗、TCR-like抗体、TCR-T等免疫治疗提供了重要的多肽靶点和TCR信息,具有实际的临床应用价值。展开更多
<em>Platycladus orientalis</em> is one of the main tree species in Mount Tai, and its sustainable development is of great significance to the protection and development of the forest ecological environment...<em>Platycladus orientalis</em> is one of the main tree species in Mount Tai, and its sustainable development is of great significance to the protection and development of the forest ecological environment of Mount Tai. In this study, a representative sample plot of <em>Platycladus orientalis</em> with a size of 30 m * 20 m was selected on Mount Tai. The growth and distribution of the parent trees and seedlings of <em>Platycladus orientalis</em> were investigated in the sample plots. The results showed that both the mother cypress trees and seedlings inside the plot present an aggregated distribution pattern within a certain scale, which conforms to the distribution of most natural communities;and the mother cypress can promote seedlings within a certain scale. Although <em>Platycladus orientalis</em> has good natural regeneration ability, it is difficult to succeed in understory regeneration solely relying on natural fertility, and it is not conducive to the long-term stable development of forest stands. Therefore, the thinning of <em>Platycladus orientalis</em> and the adjustment of the forest stand structure and artificial promotion of natural regeneration can not only reduce operating costs, but also conform to the idea of forest near-natural management and maintain sustainable forest development.展开更多
Staphylococcus aureus is a gram-staining positive cocci bacillus baterium and also one of the foodborne pathogens, which is a serious potential hazard to human health and food safety. We constructed an electroche...Staphylococcus aureus is a gram-staining positive cocci bacillus baterium and also one of the foodborne pathogens, which is a serious potential hazard to human health and food safety. We constructed an electrochemical biosensor for the detection of S. aureus based on nucleic acid aptamers to achieve highly specific detection of S. aureus. The detection of S. aureus was realized by using Aptamer (Apt) to capture S. aureus, which resulted in a change in the spatial conformation of Apt and a decrease in the electrochemical signal. Under the optimized experimental conditions, the detected electrochemical signals were positively correlated with the concentration of S. aureus with a linear range of 1 × 10<sup>1</sup> - 1 × 10<sup>5</sup> CFU/mL, a detection limit of 4.76 CFU/mL, and an experimental recovery of 97.43% - 99.37%. Therefore, we successfully constructed an electrochemical biosensor for the specific detection of S. aureus, which has the advantages of high specificity, sensitive detection and convenient operation.展开更多
<div style="text-align:justify;"> Based on the Unsteady Reynolds-Averaged Navier-Stokes (URANS) method, this paper studied the effect of the nose shape on the aerodynamic performance when the high-spee...<div style="text-align:justify;"> Based on the Unsteady Reynolds-Averaged Navier-Stokes (URANS) method, this paper studied the effect of the nose shape on the aerodynamic performance when the high-speed train subjected to a windbreak transition under crosswinds. The windbreak transition generated by the irregular terrain from the flat ground to the cutting. The results showed that with the height of the front window increased from Z ? 2 to Z + 2 (the dimensionless height), the side force coefficient <em>C</em><em><sub>y</sub><sup> </sup></em>and rolling moment co-efficient <em>C</em><sub><em>mx </em></sub>increased by 26% and 27% for the head car, respectively. The flow structures around the lower front window were smoother than that around the higher front window. The flow structures in the higher front window resulted in more considerable positive pressure on the windward side (WWS) and top of the nose region. </div>展开更多
基金supported by the National Key R&D Program of China(2022YFA1404000,2021YFA1402004,and 2022YFA1405300)the National Natural Science Foundation of China(61827824,61975104,12225405,U20A2074,U20A20218,61525504,and 61435011)+3 种基金the Innovation Program for Quantum Science and Technology(2021ZD0301700)the Fund for Science and Technology on Electronic Information Control Laboratory and the Fund for Shanxi“331 Project”Key Subjects Construction,Bairen Project of Shanxi Province,China,the Anhui Initiative in Quantum Information Technologies(AHY020200)the Major Science and Technology Projects in Anhui Province(202203a13010001)National Research Foundation,Prime Ministers Office,Singapore and the Ministry of Education,Singapore under the Research Centres of Excellence programme.
文摘Rydberg atoms-based electric field sensing has developed rapidly over the past decade.A variety of theoretical proposals and experiment configurations are suggested and realized to improve the measurement metrics,such as intensity sensitivity,bandwidth,phase,and accuracy.The Stark effect and electromagnetically induced transparency(EIT)or electromagnetically induced absorption(EIA)are fundamental physics principles behind the stage.Furthermore,various techniques such as amplitude-or frequency-modulation,optical homodyne read-out,microwave superheterodyne and frequency conversion based on multi-wave mixing in atoms are utilized to push the metrics into higher levels.In this review,different technologies and the corresponding metrics they had achieved were presented,hoping to inspire more possibilities in the improvement of metrics of Rydberg atom-based electric field sensing and broadness of application scenarios.
文摘构建随机ssDNA文库,通过SELEX技术,以正常、炎性宫颈脱落细胞为反筛细胞,以上皮内低级别病变(CIN1)、上皮内高级别病变(CIN2、CIN3)和鳞状细胞癌脱落细胞为正筛细胞,经过12轮筛选特异性适配子高度富集得到宫颈癌前病变适配子库,经特异性、亲和力分析和细胞免疫荧光确立高特异性适配子CIN-Ap4可作为诊断宫颈癌前病变生物标志物,为宫颈癌前病变分子诊断奠定理论基础,提供新思路。利用Prime Premier 5.0设计构建了随机ssDNA文库并根据文库两端固定序列设计引物,对对称PCR和间接不对称PCR中的退火温度、循环数以及上、下游引物浓度比等条件进行优化,分析确定50μL反应体系中对称PCR的最佳反应条件为:最佳退火温度为49.5℃,最佳循环数为15个循环;间接不对称PCR的最佳反应条件为:50μL反应体系中上、下游引物浓度的最佳比例为80∶1,最佳循环数为35个循环。实验结果表明成功构建了寡核苷酸文库,在最适PCR条件下可获得理想的dsDNA和ssDNA,并具有良好的重复性,为顺利筛选适配子提供保证。
基金Supported by:Science-Technology Support Plan Projects of Gansu Province(No.18JR3RA275)Science-Technology Support Plan Projects of Lanzhou Chengguan District(No.2018SHFZ0048)~~
文摘结直肠癌是世界高发和高致死率的恶性肿瘤。靶向新抗原的免疫治疗已被证实可以诱导癌症患者肿瘤持续消退,但这些特异性新抗原,仅适用于个体精准治疗。随着大量的高频肿瘤基因突变被发现,这些与突变相关的高频新抗原可覆盖更多人群,具有较强的临床意义。然而目前结直肠癌中是否也存在高频新抗原仍不清楚。本研究利用来源于321个结直肠癌患者的体细胞突变数据库,联合1种标准过滤和7种预测算法,筛选并获得了25个基于中国人高频分型HLA-A^*1101限制性的高频新抗原,它们均具有高亲和力(IC50<50 nmol/L)和高呈递分值(>0.90);其中,除了阳性对照多肽KRAS_G12V8-16外,11个高频新抗原能够在体外诱导细胞毒性T淋巴细胞(cytotoxic T lymphocyte,CTL)分泌γ干扰素(interferon gamma,IFN-γ),证实具有免疫原性。选取免疫原性最强的新抗原C1orf170_S418G413-421及阳性对照多肽KRAS_G12V8-16体外刺激T细胞,利用流式细胞分选及单细胞转录组测序技术,获得其特异性CTL的免疫组库信息,所构建的TCR-T(T-cell receptor engineered T cell)能够识别新抗原并分泌细胞因子。以上结果表明,本研究开发了一种利用体细胞数据库预测并体外筛选验证具有免疫原性高频新抗原的方法,为结直肠癌及其他癌种的多肽、DC(dendritic cells)疫苗、TCR-like抗体、TCR-T等免疫治疗提供了重要的多肽靶点和TCR信息,具有实际的临床应用价值。
文摘<em>Platycladus orientalis</em> is one of the main tree species in Mount Tai, and its sustainable development is of great significance to the protection and development of the forest ecological environment of Mount Tai. In this study, a representative sample plot of <em>Platycladus orientalis</em> with a size of 30 m * 20 m was selected on Mount Tai. The growth and distribution of the parent trees and seedlings of <em>Platycladus orientalis</em> were investigated in the sample plots. The results showed that both the mother cypress trees and seedlings inside the plot present an aggregated distribution pattern within a certain scale, which conforms to the distribution of most natural communities;and the mother cypress can promote seedlings within a certain scale. Although <em>Platycladus orientalis</em> has good natural regeneration ability, it is difficult to succeed in understory regeneration solely relying on natural fertility, and it is not conducive to the long-term stable development of forest stands. Therefore, the thinning of <em>Platycladus orientalis</em> and the adjustment of the forest stand structure and artificial promotion of natural regeneration can not only reduce operating costs, but also conform to the idea of forest near-natural management and maintain sustainable forest development.
文摘Staphylococcus aureus is a gram-staining positive cocci bacillus baterium and also one of the foodborne pathogens, which is a serious potential hazard to human health and food safety. We constructed an electrochemical biosensor for the detection of S. aureus based on nucleic acid aptamers to achieve highly specific detection of S. aureus. The detection of S. aureus was realized by using Aptamer (Apt) to capture S. aureus, which resulted in a change in the spatial conformation of Apt and a decrease in the electrochemical signal. Under the optimized experimental conditions, the detected electrochemical signals were positively correlated with the concentration of S. aureus with a linear range of 1 × 10<sup>1</sup> - 1 × 10<sup>5</sup> CFU/mL, a detection limit of 4.76 CFU/mL, and an experimental recovery of 97.43% - 99.37%. Therefore, we successfully constructed an electrochemical biosensor for the specific detection of S. aureus, which has the advantages of high specificity, sensitive detection and convenient operation.
文摘<div style="text-align:justify;"> Based on the Unsteady Reynolds-Averaged Navier-Stokes (URANS) method, this paper studied the effect of the nose shape on the aerodynamic performance when the high-speed train subjected to a windbreak transition under crosswinds. The windbreak transition generated by the irregular terrain from the flat ground to the cutting. The results showed that with the height of the front window increased from Z ? 2 to Z + 2 (the dimensionless height), the side force coefficient <em>C</em><em><sub>y</sub><sup> </sup></em>and rolling moment co-efficient <em>C</em><sub><em>mx </em></sub>increased by 26% and 27% for the head car, respectively. The flow structures around the lower front window were smoother than that around the higher front window. The flow structures in the higher front window resulted in more considerable positive pressure on the windward side (WWS) and top of the nose region. </div>
