Background： The 47,XYY syndrome could result in fertility problems. However, seldom studies reported comprehensive researches on the embryonic development and pregnancy outcomes of these patients. This study aimed to...Background： The 47,XYY syndrome could result in fertility problems. However, seldom studies reported comprehensive researches on the embryonic development and pregnancy outcomes of these patients. This study aimed to evaluate the clinical outcomes of nonmosaic 47,XYY patients performed with fluorescent in situ hybridization （FISH） and preimplantation genetic diagnosis （PGD） treatment. Methods： This was a retrospective study. Between January 2012 and May 2017, 51 infertile males with nonmosaic 47,XYY syndrome underwent FISH-PGD were included in the study. According to sex chromosomal FISH results, embryos were classified as normal signal, no nuclei fixed, no signal in fixed nuclei, suspensive signal, and abnormal signal groups, respectively. The incidence of each group, the fixation rate, and hybridization rate were calculated. Embryonic development and pregnancy outcomes were also analyzed. The measurement data were analyzed with Student＇s t-test. The comparison of categorical data was analyzed with the Chi-square test and Fisher＇s exact test when expected cell count was 〈5. Results： The 53 PGD cycles with 433 embryos were analyzed. The fixation rate was 89.6%, while the hybridization rate was 96.4%. There were 283 embryos with two sex chromosomal signals with clear diagnosis （65.4%）. The numbers of no nuclei fixed, no signal in fixed nuclei, suspensive signal, and abnormal signal groups were 45 （10.4%）, 14 （3.2%）, 24 （5.5%）, and 67 （15.5%）, respectively. Embryos with abnormal signals were abandoned. The number of good-quality embryos was 210 （57.4%）, including implanted embryos on day 4/day 5 a.ld cryopreserved. The rates of good-quality embryos in the no nuclei fixed （22.2%）, no signal in fixed nuclei （28.6%）, and suspensive signal groups （33.3%） were comparable （P 〉 0.05）, and were significantly lower than the normal signal group （66.4%, P 〈 0.001 ）. The clinical pregnancy rates of fresh and frozen embryos transferred cycles were 70.6% and 85.展开更多
目的分析胎盘间充质干细胞移植对类风湿关节炎大鼠炎症因子及软骨破坏的改善作用。方法将32只SD大鼠随机分为4组,实验组、对照组、模型组及对照组,每组8只。实验组、对照组和模型组为复制类风湿关节炎动物模型,正常组不做任何处理(n=8)...目的分析胎盘间充质干细胞移植对类风湿关节炎大鼠炎症因子及软骨破坏的改善作用。方法将32只SD大鼠随机分为4组,实验组、对照组、模型组及对照组,每组8只。实验组、对照组和模型组为复制类风湿关节炎动物模型,正常组不做任何处理(n=8)。造模后12 h,实验组尾静脉注射胎盘间充质干细胞,对照组注射等量鼠成纤维细胞,模型组不干预。干预3周后,处死所有大鼠,酶联免疫吸附实验与q RT-PCR检测。结果 (1)模型组、对照组与正常组比较,TNF-α、IL-1β及IL-6水平均升高,差异有统计学意义(P<0.05);实验组与模型组及对照组比较,TNF-α、IL-1β及IL-6水平降低,差异有统计学意义(P<0.05);实验组转化生长因子β(TGF-β)水平高于其余各组,差异有统计学意义(P<0.05);(2)模型组、对照组与正常组比较,滑膜组织基质金属蛋白酶1(MMP-1)、基质金属蛋白酶3(MMP-3)及基质金属蛋白酶13(MMP-13)m RNA升高,差异有统计学意义(P<0.05);实验组与对照组及模型组比较,MMP-1、MMP-3及MMP-13m RNA水平降低,差异有统计学意义(P<0.05)。实验组、对照组、模型组与正常组比较,基质金属蛋白酶抑制物3 m RNA降低,差异有统计学意义(P<0.05)。模型组、对照组与正常组比较,钙黏素11 m RNA升高,差异有统计学意义(P<0.05);实验组与模型组、对照组比较,钙黏素11水平降低,差异有统计学意义(P<0.05)。结论胎盘间充质干细胞可能通过抑制TNF-α、IL-1β、IL-6水平及MMP分泌与钙黏素11表达,上调TGF-β水平,来减轻类风湿关节炎大鼠的关节炎症与软骨破坏。展开更多
基金This study was supported by grants form Nationl Key Research and Development Program（No.2016YFC1000202）,National Natural Science Foundation of China（No.81671522）,and Shandong Medical and Health Science and Technology Development Plan （No.2016WS0370）.
文摘Background： The 47,XYY syndrome could result in fertility problems. However, seldom studies reported comprehensive researches on the embryonic development and pregnancy outcomes of these patients. This study aimed to evaluate the clinical outcomes of nonmosaic 47,XYY patients performed with fluorescent in situ hybridization （FISH） and preimplantation genetic diagnosis （PGD） treatment. Methods： This was a retrospective study. Between January 2012 and May 2017, 51 infertile males with nonmosaic 47,XYY syndrome underwent FISH-PGD were included in the study. According to sex chromosomal FISH results, embryos were classified as normal signal, no nuclei fixed, no signal in fixed nuclei, suspensive signal, and abnormal signal groups, respectively. The incidence of each group, the fixation rate, and hybridization rate were calculated. Embryonic development and pregnancy outcomes were also analyzed. The measurement data were analyzed with Student＇s t-test. The comparison of categorical data was analyzed with the Chi-square test and Fisher＇s exact test when expected cell count was 〈5. Results： The 53 PGD cycles with 433 embryos were analyzed. The fixation rate was 89.6%, while the hybridization rate was 96.4%. There were 283 embryos with two sex chromosomal signals with clear diagnosis （65.4%）. The numbers of no nuclei fixed, no signal in fixed nuclei, suspensive signal, and abnormal signal groups were 45 （10.4%）, 14 （3.2%）, 24 （5.5%）, and 67 （15.5%）, respectively. Embryos with abnormal signals were abandoned. The number of good-quality embryos was 210 （57.4%）, including implanted embryos on day 4/day 5 a.ld cryopreserved. The rates of good-quality embryos in the no nuclei fixed （22.2%）, no signal in fixed nuclei （28.6%）, and suspensive signal groups （33.3%） were comparable （P 〉 0.05）, and were significantly lower than the normal signal group （66.4%, P 〈 0.001 ）. The clinical pregnancy rates of fresh and frozen embryos transferred cycles were 70.6% and 85.
文摘目的分析胎盘间充质干细胞移植对类风湿关节炎大鼠炎症因子及软骨破坏的改善作用。方法将32只SD大鼠随机分为4组,实验组、对照组、模型组及对照组,每组8只。实验组、对照组和模型组为复制类风湿关节炎动物模型,正常组不做任何处理(n=8)。造模后12 h,实验组尾静脉注射胎盘间充质干细胞,对照组注射等量鼠成纤维细胞,模型组不干预。干预3周后,处死所有大鼠,酶联免疫吸附实验与q RT-PCR检测。结果 (1)模型组、对照组与正常组比较,TNF-α、IL-1β及IL-6水平均升高,差异有统计学意义(P<0.05);实验组与模型组及对照组比较,TNF-α、IL-1β及IL-6水平降低,差异有统计学意义(P<0.05);实验组转化生长因子β(TGF-β)水平高于其余各组,差异有统计学意义(P<0.05);(2)模型组、对照组与正常组比较,滑膜组织基质金属蛋白酶1(MMP-1)、基质金属蛋白酶3(MMP-3)及基质金属蛋白酶13(MMP-13)m RNA升高,差异有统计学意义(P<0.05);实验组与对照组及模型组比较,MMP-1、MMP-3及MMP-13m RNA水平降低,差异有统计学意义(P<0.05)。实验组、对照组、模型组与正常组比较,基质金属蛋白酶抑制物3 m RNA降低,差异有统计学意义(P<0.05)。模型组、对照组与正常组比较,钙黏素11 m RNA升高,差异有统计学意义(P<0.05);实验组与模型组、对照组比较,钙黏素11水平降低,差异有统计学意义(P<0.05)。结论胎盘间充质干细胞可能通过抑制TNF-α、IL-1β、IL-6水平及MMP分泌与钙黏素11表达,上调TGF-β水平,来减轻类风湿关节炎大鼠的关节炎症与软骨破坏。
