首先回顾了计算视觉发展的历史,介绍了神经元、多层感知机和反向传播等人工神经网络的基本知识以及卷积神经网络的发展史及其卷积、池化等基本操作;讨论了Alex Net、VGGNet、Goog Le Net和Res Net等经典卷积神经网络结构,并重点介绍了Ca...首先回顾了计算视觉发展的历史,介绍了神经元、多层感知机和反向传播等人工神经网络的基本知识以及卷积神经网络的发展史及其卷积、池化等基本操作;讨论了Alex Net、VGGNet、Goog Le Net和Res Net等经典卷积神经网络结构,并重点介绍了CapsNet;总结了卷积神经网络在图像分类、语义分割、目标检测以及图像生成等领域的研究进展;最后提出了卷积神经网络研究所面临的挑战以及对CapsNet未来研究的展望。展开更多
Background: Following the intake of Fusarium mycotoxin-contaminated feed,small intestines may be exposed to high levels of toxic substances that can potentially damage intestinal functions in livestock.It is well know...Background: Following the intake of Fusarium mycotoxin-contaminated feed,small intestines may be exposed to high levels of toxic substances that can potentially damage intestinal functions in livestock.It is well known that Fusarium mycotoxins will lead a breakdown of the normally impeccable epithelial barrier,resulting in the development of a "leaky" gut.H2 administration with different methods has been proved definitely potentials to prevent serious intestinal diseases.The goal of this study is to investigate the roles of lactulose(LAC) and hydrogenrich water(HRW) in preventing intestinal dysfunction in piglets fed Fusarium mycotoxin-contaminated feed.Methods: A total of 24 female piglets were evenly assigned to 4 groups: negative control(NC) group,mycotoxincontaminated(MC) feed group,MC feed with LAC treatment(MC + LAC),and MC feed with HRW treatment(MC +HRW),respectively.Piglets in the NC group were fed uncontaminated control diet,while remaining piglets were fed Fusarium mycotoxin-contaminated diet.For the NC and MC groups,10 mL/kg body weight(BW) of hydrogen-free water(HFW) was orally administrated to piglets twice daily;while in the MC + LAC and MC + HRW groups,piglets were treated with the same dose of LAC solution(500 mg/kg BW) and HRW twice daily,respectively.On d 25,serum was collected and used for biochemical analysis.Intestinal tissues were sampled for morphological examination as well as relative genes and protein expression analysis.Results: Our data showed that Fusarium mycotoxins induced higher serum diamine oxidase(DAO) activities(P < 0.05),D-lactic acid levels(P < 0.01),and endotoxin status(P < 0.01),lower villus height(P < 0.01) and ratio of villus height to crypt depth(P < 0.05) in small intestine,greater apoptosis index and higher mRNA expression related to tight junctions(P < 0.05).In addition,the distribution and down-regulation of claudin-3(CLDN3) protein in the small intestinal was also observed.As expected,oral administrations of HRW and LAC were found to remarkably provide beneficial e展开更多
Background:Heat stress(HS)jeopardizes intestinal barrier functions and augments intestinal permeability in pigs.However,whether HS-induced maternal microbial and metabolic changes in primiparous sows during late gesta...Background:Heat stress(HS)jeopardizes intestinal barrier functions and augments intestinal permeability in pigs.However,whether HS-induced maternal microbial and metabolic changes in primiparous sows during late gestation remains elusive.We present here,a study investigating the fecal microbial and metabolic responses in late gestational primiparous sows when exposed to HS.Methods:Twelve first-parity Landrace×Large White F1 sows were randomly assigned into two environmental treatments including the thermoneutral(TN)(18–22℃;n=6)and HS(28–32℃;n=6)conditions.Both treatments were applied from 85 d of gestation to farrowing.The serum and feces samples were collected on d 107 of gestation,for analyses including intestinal integrity biomarkers,high-throughput sequencing metagenomics,short-chain fatty acid(SCFA)profiles and nontargeted metabolomics.Results:Our results show that HS group has higher serum Heat shock protein 70(HSP70),lipopolysaccharide(LPS)and lipopolysaccharide-binding protein(LBP)levels.The gut microbial community can be altered upon HS by usingβ-diversity and taxon-based analysis.In particular,the relative abundance of genera and operational taxonomic units(OTUs)related to Clostridiales and Halomonas are higher in HS group,the relative abundance of genera and OTUs related to Bacteroidales and Streptococcus,however,are lower in HS group.Results of metabolic analysis reveal that HS lowers the concentrations of propionate,butyrate,total SCFA,succinate,fumarate,malate,lactate,aspartate,ethanolamine,β-alanine and niacin,whereas that of fructose and azelaic acid are higher in HS group.These metabolites mainly affect propanoate metabolism,alanine,aspartate and glutamate metabolism,phenylalanine metabolism,β-alanine metabolism,pantothenate and CoA biosynthesis,tricarboxylic acid cycle(TCA)and nicotinate and nicotinamide metabolism.Additionally,correlation analysis between significant microbes and metabolites indicated that the HS-induced microbiota shift is likely the cause of changes of intestinal 展开更多
Background Lactulose as an effective prebiotic protects intestinal mucosal injury.Bacillus coagulans is widely used in feed additives because of its ability to promote intestinal health.Our previous study suggests tha...Background Lactulose as an effective prebiotic protects intestinal mucosal injury.Bacillus coagulans is widely used in feed additives because of its ability to promote intestinal health.Our previous study suggests that the combination of lactulose and Bacillus coagulans may be a good candidate as alternative for antibiotic growth promoters.However,the in vivo effects of lactulose and Bacillus coagulans on growth and intestinal health under immune challenge in piglets remains unclear.The objective of this study is to explore the protective effects of synbiotic containing lactu-lose and Bacillus coagulans on the intestinal mucosal injury and barrier dysfunction under immune challenge in weaned piglets.Methods Twenty four weaned piglets were assigned to 4 groups.Piglets in the CON-_(saline)and LPS-_(LPS)group were fed the basal diet,while others were fed either with chlortetracycline(CTC)or synbiotic mixture of lactulose and Bacillus coagulans for 32 d before injection of saline or lipopolysaccharide(LPS).Piglets were sacrificed 4 h after LPS injection to collect samples to determine intestinal morphology,integrity and barrier functions as well as relative genes and proteins.Results Our data showed that no differences were observed in the growth performance of the four test groups.LPS injection induced higher serum diamine oxidase activities,D-lactic acid levels,and endotoxin status,lower villus height and ratio of villus height to crypt depth,greater mRNA and lower protein expression related tight junction in both jejunum and ileum.In addition,a higher apoptosis index,and protein expression of Bax and caspase-3 were also observed in the LPS challenge group.Interestingly,dietary synbiotic mixture with lactulose and Bacillus coagulans protected against LPS-induced intestinal damage,barrier dysfunction and higher apoptosis as well as CTC.Conclusions Our data suggest that dietary supplementation of synbiotic mixture with lactulose and Bacillus coagu-lans showed resilience to LPS-induced intestinal morphological damage,展开更多
Considering the long length of gas‐insulated transmission lines(GILs),novel methods are required to alleviate the impacts of axial diffusion on decomposition products'moni-toring.The diffusion process of two typi...Considering the long length of gas‐insulated transmission lines(GILs),novel methods are required to alleviate the impacts of axial diffusion on decomposition products'moni-toring.The diffusion process of two typical decomposition products,SO2 and SO2F2,was studied.During the diffusion experiments,SO2 and SO2F2 were generated by partial discharges.Based on Fick’s law,an axial diffusion model of SO2 and SO2F2 was proposed.By combining the experiment and simulation results,it was found that the diffusion coefficient of SO2 was greater than that of SO2F2.In addition,the concentration variation at different positions would experience some delay and decay in axial direction.The delay and decay were inversely related to the diffusion coefficient and increased with prolongation of the distance.Based on the diffusion characteristics determined,methods for optimising the monitoring positions,correcting concentration,and using the concentration ratio and generation rate were proposed to alleviate the impacts of diffusion.The methods could allow the decomposition products to be monitored in 1 month and reduce the monitoring error to 10%or less.The diffusion model and the methods could be useful for monitoring decomposition products in GILs.展开更多
文摘首先回顾了计算视觉发展的历史,介绍了神经元、多层感知机和反向传播等人工神经网络的基本知识以及卷积神经网络的发展史及其卷积、池化等基本操作;讨论了Alex Net、VGGNet、Goog Le Net和Res Net等经典卷积神经网络结构,并重点介绍了CapsNet;总结了卷积神经网络在图像分类、语义分割、目标检测以及图像生成等领域的研究进展;最后提出了卷积神经网络研究所面临的挑战以及对CapsNet未来研究的展望。
基金supported by grants from the National Nature Science Foundation of China(31501986)Fundamental Research Funds for Central Universities(KJQN201611 and KYDS201809)Earmarked Fund for Jiangsu Agricultural Industry Technology System(SXGC[2018]287)
文摘Background: Following the intake of Fusarium mycotoxin-contaminated feed,small intestines may be exposed to high levels of toxic substances that can potentially damage intestinal functions in livestock.It is well known that Fusarium mycotoxins will lead a breakdown of the normally impeccable epithelial barrier,resulting in the development of a "leaky" gut.H2 administration with different methods has been proved definitely potentials to prevent serious intestinal diseases.The goal of this study is to investigate the roles of lactulose(LAC) and hydrogenrich water(HRW) in preventing intestinal dysfunction in piglets fed Fusarium mycotoxin-contaminated feed.Methods: A total of 24 female piglets were evenly assigned to 4 groups: negative control(NC) group,mycotoxincontaminated(MC) feed group,MC feed with LAC treatment(MC + LAC),and MC feed with HRW treatment(MC +HRW),respectively.Piglets in the NC group were fed uncontaminated control diet,while remaining piglets were fed Fusarium mycotoxin-contaminated diet.For the NC and MC groups,10 mL/kg body weight(BW) of hydrogen-free water(HFW) was orally administrated to piglets twice daily;while in the MC + LAC and MC + HRW groups,piglets were treated with the same dose of LAC solution(500 mg/kg BW) and HRW twice daily,respectively.On d 25,serum was collected and used for biochemical analysis.Intestinal tissues were sampled for morphological examination as well as relative genes and protein expression analysis.Results: Our data showed that Fusarium mycotoxins induced higher serum diamine oxidase(DAO) activities(P < 0.05),D-lactic acid levels(P < 0.01),and endotoxin status(P < 0.01),lower villus height(P < 0.01) and ratio of villus height to crypt depth(P < 0.05) in small intestine,greater apoptosis index and higher mRNA expression related to tight junctions(P < 0.05).In addition,the distribution and down-regulation of claudin-3(CLDN3) protein in the small intestinal was also observed.As expected,oral administrations of HRW and LAC were found to remarkably provide beneficial e
文摘二维码在产品防伪、追溯等领域中广泛应用,数字水印不仅能有效保护其所属权,而且不影响其正常使用。水印在二维码中具有不可见性、鲁棒性。首先对二维码图像进行分块,并对每个分块进行离散余弦变换(Discrete Cosine Transform,DCT);其次对每一分块再取中心块进行奇异值分解(Singular Value Decomposition,SVD);最后将经Logistic和Arnold变换加密后的水印图像置入到奇异值分解后的特征值中,形成置入水印的二维码。二维码置入水印的逆过程实现了水印的提取。实验证实了二维码中水印能有效抵抗图像多种攻击,具有良好的鲁棒性、安全性和不可见性。
基金supported by National Key R&D Program of China(2016YFD0500502)Earmarked Fund for Jiangsu Modern Agricultural(Swine)Industry Technology System(JATS[2018]287)Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX18_0698).
文摘Background:Heat stress(HS)jeopardizes intestinal barrier functions and augments intestinal permeability in pigs.However,whether HS-induced maternal microbial and metabolic changes in primiparous sows during late gestation remains elusive.We present here,a study investigating the fecal microbial and metabolic responses in late gestational primiparous sows when exposed to HS.Methods:Twelve first-parity Landrace×Large White F1 sows were randomly assigned into two environmental treatments including the thermoneutral(TN)(18–22℃;n=6)and HS(28–32℃;n=6)conditions.Both treatments were applied from 85 d of gestation to farrowing.The serum and feces samples were collected on d 107 of gestation,for analyses including intestinal integrity biomarkers,high-throughput sequencing metagenomics,short-chain fatty acid(SCFA)profiles and nontargeted metabolomics.Results:Our results show that HS group has higher serum Heat shock protein 70(HSP70),lipopolysaccharide(LPS)and lipopolysaccharide-binding protein(LBP)levels.The gut microbial community can be altered upon HS by usingβ-diversity and taxon-based analysis.In particular,the relative abundance of genera and operational taxonomic units(OTUs)related to Clostridiales and Halomonas are higher in HS group,the relative abundance of genera and OTUs related to Bacteroidales and Streptococcus,however,are lower in HS group.Results of metabolic analysis reveal that HS lowers the concentrations of propionate,butyrate,total SCFA,succinate,fumarate,malate,lactate,aspartate,ethanolamine,β-alanine and niacin,whereas that of fructose and azelaic acid are higher in HS group.These metabolites mainly affect propanoate metabolism,alanine,aspartate and glutamate metabolism,phenylalanine metabolism,β-alanine metabolism,pantothenate and CoA biosynthesis,tricarboxylic acid cycle(TCA)and nicotinate and nicotinamide metabolism.Additionally,correlation analysis between significant microbes and metabolites indicated that the HS-induced microbiota shift is likely the cause of changes of intestinal
基金supported by the National Key R&D Program of China(2017YFE0114400).
文摘Background Lactulose as an effective prebiotic protects intestinal mucosal injury.Bacillus coagulans is widely used in feed additives because of its ability to promote intestinal health.Our previous study suggests that the combination of lactulose and Bacillus coagulans may be a good candidate as alternative for antibiotic growth promoters.However,the in vivo effects of lactulose and Bacillus coagulans on growth and intestinal health under immune challenge in piglets remains unclear.The objective of this study is to explore the protective effects of synbiotic containing lactu-lose and Bacillus coagulans on the intestinal mucosal injury and barrier dysfunction under immune challenge in weaned piglets.Methods Twenty four weaned piglets were assigned to 4 groups.Piglets in the CON-_(saline)and LPS-_(LPS)group were fed the basal diet,while others were fed either with chlortetracycline(CTC)or synbiotic mixture of lactulose and Bacillus coagulans for 32 d before injection of saline or lipopolysaccharide(LPS).Piglets were sacrificed 4 h after LPS injection to collect samples to determine intestinal morphology,integrity and barrier functions as well as relative genes and proteins.Results Our data showed that no differences were observed in the growth performance of the four test groups.LPS injection induced higher serum diamine oxidase activities,D-lactic acid levels,and endotoxin status,lower villus height and ratio of villus height to crypt depth,greater mRNA and lower protein expression related tight junction in both jejunum and ileum.In addition,a higher apoptosis index,and protein expression of Bax and caspase-3 were also observed in the LPS challenge group.Interestingly,dietary synbiotic mixture with lactulose and Bacillus coagulans protected against LPS-induced intestinal damage,barrier dysfunction and higher apoptosis as well as CTC.Conclusions Our data suggest that dietary supplementation of synbiotic mixture with lactulose and Bacillus coagu-lans showed resilience to LPS-induced intestinal morphological damage,
基金supported by the National Natural Science Foundation of China(U1966211)the Science and Technology Project of Central China Branch of State Grid Corporation of China(SGHZ0000AJJS2000292).
文摘Considering the long length of gas‐insulated transmission lines(GILs),novel methods are required to alleviate the impacts of axial diffusion on decomposition products'moni-toring.The diffusion process of two typical decomposition products,SO2 and SO2F2,was studied.During the diffusion experiments,SO2 and SO2F2 were generated by partial discharges.Based on Fick’s law,an axial diffusion model of SO2 and SO2F2 was proposed.By combining the experiment and simulation results,it was found that the diffusion coefficient of SO2 was greater than that of SO2F2.In addition,the concentration variation at different positions would experience some delay and decay in axial direction.The delay and decay were inversely related to the diffusion coefficient and increased with prolongation of the distance.Based on the diffusion characteristics determined,methods for optimising the monitoring positions,correcting concentration,and using the concentration ratio and generation rate were proposed to alleviate the impacts of diffusion.The methods could allow the decomposition products to be monitored in 1 month and reduce the monitoring error to 10%or less.The diffusion model and the methods could be useful for monitoring decomposition products in GILs.
