The concept of finite-time stability for linear singular system is induced in this paper.Finite-time control problem is considered for linear singular systems with time-varying parametricuncertainties and exogenous di...The concept of finite-time stability for linear singular system is induced in this paper.Finite-time control problem is considered for linear singular systems with time-varying parametricuncertainties and exogenous disturbances. The disturbance satisfies a dynamical system with para-metric uncertainties. A su?cient condition is presented for robust finite-time stabilization via statefeedback. The condition is translated to a feasibility problem involving restricted linear matrix in-equalities (LMIs). A detailed solving method is proposed for the restricted linear matrix inequalities.Finally, an example is given to show the validity of the results.展开更多
In this paper, we use the solutions of forward-backward stochastic differential equations to get the explicit form of the optimal control for linear quadratic stochastic optimal control problem and the open-loop Nash ...In this paper, we use the solutions of forward-backward stochastic differential equations to get the explicit form of the optimal control for linear quadratic stochastic optimal control problem and the open-loop Nash equilibrium point for nonzero sum differential games problem. We also discuss the solvability of the generalized Riccati equation system and give the linear feedback regulator for the optimal control problem using the solution of this kind of Riccati equation system.展开更多
Background The role of extracellular signal-regulated kinase 1/2 (ERK1/2) in shikonin-induced HeLa cells apoptosis remains vague. This study was to investigate the activation of caspase pathways and the role of E...Background The role of extracellular signal-regulated kinase 1/2 (ERK1/2) in shikonin-induced HeLa cells apoptosis remains vague. This study was to investigate the activation of caspase pathways and the role of ERK1/2 in human cervical cancer cells, HeLa, by shikonin. Methods The inhibitory effect of shikonin on the growth of HeLa cells was measured by MTT assay. Fluorescent microscopic analysis of apoptotic cells stained with 4’,6’-oliiamiclino-2-phenylindole C (DAPI) and Hoechst 33258 was carried out. Caspase-3 and -8 activities were detected using caspase-3 substrate and caspase-8 substrate as substrates, respectively. The protein levels of ERK, p53 and p-ERK were determined by Western blot analysis.Results Shikonin inhibited cell growth in a time- and dose-dependent manner. Caspase-3 and caspase-8 were activated in the apoptotic process and caspase inhibitors effectively reversed shikonin-induced apoptosis. Phosphorylation of ERK resulted in up-regulation of p53 expression, which was blocked by mitogen-activated protein kinase (MEK), inhibitor PD 98059.Conclusion Shikonin induces HeLa cell apoptosis through the ERK, p53 and caspase pathways.展开更多
creening of foodborne pathogens is important to prevent contaminated foods from their supply chains.n this study, a portable detection device was developed for rapid, sensitive and simple detection of viable almonella...creening of foodborne pathogens is important to prevent contaminated foods from their supply chains.n this study, a portable detection device was developed for rapid, sensitive and simple detection of viable almonella using a finger-actuated microfluidic chip and an improved recombinase aided amplification (RAA) assay. Improved propidium monoazide(PMAxx) was combined with RAA to enable this device to distinguish viable bacteria from dead ones. The modification of PMAxx into dead bacteria, the magnetic xtraction of nucleic acids from viable bacteria and the RAA detection of extracted nucleic acids were performed using the microfluidic chip on its supporting device by finger press-release operations. The fluorescent signal resulting from RAA amplification of the nucleic acids was collected using a USB camera nd analyzed using a self-developed smartphone App to quantitatively determine the bacterial concenration. This device could detect Salmonella typhimurium in spiked chicken meats from 1.3 × 10^(2) CFU/m L o 1.3 × 10^(7) CFU/m L in 2 h with a lower detection limit of 130 CFU/m L, and has shown its potential for on-site detection of foodborne pathogens.展开更多
Background Co-salient object detection(Co-SOD)aims to identify and segment commonly salient objects in a set of related images.However,most current Co-SOD methods encounter issues with the inclusion of irrelevant info...Background Co-salient object detection(Co-SOD)aims to identify and segment commonly salient objects in a set of related images.However,most current Co-SOD methods encounter issues with the inclusion of irrelevant information in the co-representation.These issues hamper their ability to locate co-salient objects and significantly restrict the accuracy of detection.Methods To address this issue,this study introduces a novel Co-SOD method with iterative purification and predictive optimization(IPPO)comprising a common salient purification module(CSPM),predictive optimizing module(POM),and diminishing mixed enhancement block(DMEB).Results These components are designed to explore noise-free joint representations,assist the model in enhancing the quality of the final prediction results,and significantly improve the performance of the Co-SOD algorithm.Furthermore,through a comprehensive evaluation of IPPO and state-of-the-art algorithms focusing on the roles of CSPM,POM,and DMEB,our experiments confirmed that these components are pivotal in enhancing the performance of the model,substantiating the significant advancements of our method over existing benchmarks.Experiments on several challenging benchmark co-saliency datasets demonstrate that the proposed IPPO achieves state-of-the-art performance.展开更多
Pattern-triggered immunity(PTI) is an essential strategy used by plants to deploy broad-spectrum resistance against pathogen attacks. Heterotrimeric G proteins have been reported to contribute to PTI.Of the three non-...Pattern-triggered immunity(PTI) is an essential strategy used by plants to deploy broad-spectrum resistance against pathogen attacks. Heterotrimeric G proteins have been reported to contribute to PTI.Of the three non-canonical EXTRA-LARGE G PROTEINs(XLGs) in Arabidopsis thaliana, XLG2 and XLG3 were shown to positively regulate immunity,but XLG1 was not considered to function in defense,based on the analysis of a weak xlg1 allele.In this study, we characterized the xlg1 xlg2 xlg3 triple knockout mutants generated from an xlg1 knockout allele. The strong xlg1 xlg2 xlg3 triple mutants compromised pathogen-associated molecular pattern(PAMP)-triggered activation of mitogen-activated protein kinases(MAPKs) and resistance to pathogen infection. The three XLGs interacted with MAPK cascade proteins involved in defense signaling, including the MAPK kinase kinases MAPKKK3 and MAPKKK5, the MAPK kinases MKK4 and MKK5, and the MAPKs MPK3 and MPK6. Expressing a constitutively active form of MKK4 restored MAPK activation and partially recovered the compromised disease resistance seen in the strong xlg1 xlg2 xlg3 triple mutant. Furthermore, mutations of all three XLGs largely restored the phenotype of the autoimmunity mutant bak1-interacting receptor-like kinase 1. Our study reveals that all three XLGs function redundantly in PAMP-triggered MAPK activation and plant immunity.展开更多
Foodborne pathogenic bacteria have been considered as a major risk factor for food safety. It is of great significance to carry out in-field screening of pathogenic bacteria to prevent the outbreaks of foodborne disea...Foodborne pathogenic bacteria have been considered as a major risk factor for food safety. It is of great significance to carry out in-field screening of pathogenic bacteria to prevent the outbreaks of foodborne diseases. In this study, a portable lab-on-a-disc platform with a microfluidic disc was developed for rapid and automatic detection of Salmonella typhimurium using a nickel nanowire(Ni NW) net for effective separation of target bacteria, horseradish peroxidase nanoflowers(HRP NFs) for efficient amplification of biological signals, and a self-developed smartphone APP for accurate analysis of colorimetric images. First,the microfluidic disc was preloaded with reagents and samples and centrifuged to form one bacterial sample column, one immune Ni NW column, one HRP NF column, two washing buffer columns and one tetramethylbenzidine(TMB) column, which were separated by air gaps. Then, a rotatable magnetic field was specifically developed to assemble the Ni NWs into a net, which was automatically controlled by a stepped motor to successively pass through the sample column for specific capture of target bacteria, the HRP NF column for specific label of target bacteria, the washing columns for effective removal of sample background and non-specific binding NFs, and the TMB column for colorimetric determination of target bacteria. The color change of TMB from colorless to blue was finally analyzed using the smartphone APP to quantitatively determine the target bacteria. This lab-on-a-disc platform could detect Salmonella typhimurium from 5.6 × 10^(1) CFU/20 μL to 5.6 × 10^(5) CFU/20 μL in 1 h with a lower detection limit of 56 CFU/20 μL. The recovery of target bacteria in spiked chicken samples ranged from 97.5% to 101.8%. This portable platform integrating separation, labeling, washing, catalysis and detection onto a single disc is featured with automatic operation, fast reaction, and small size and has shown its potential for in-field detection of foodborne pathogens.展开更多
An LCC delivery system for Fenofibrate (Fen) was developed to improve its poorly oral bioavailability. Fen-LCC preparation methods were screened, and the prepared Fen-LCC was then characterized by a polarizing microsc...An LCC delivery system for Fenofibrate (Fen) was developed to improve its poorly oral bioavailability. Fen-LCC preparation methods were screened, and the prepared Fen-LCC was then characterized by a polarizing microscope and transmission electron microscopy (TEM).The spray drying technique was selected to dry and solidify particles into powder. The in vitro release of Fen-LCC was measured and in vivo pharmacokinetic experiments were carried out on rats after oral administration. Particles prepared through the high-temperature input method exhibited structural characteristics of LCC, and re-dissolved particles maintained the same features. The LCC delivery system can significantly improve in vitro release outcomes. After oral administration, AUCs of the suspension and LCC systems were measured at 131.6853 μg·h/ml and 1435.72893 μg·h/ml, respectively. The spray drying process presented here better maintains cubic structures, and the LCC system significantly improves bioavailability levels.展开更多
In November 2012,diarrhea-predominant disease broke out in a large rabbit farm in Huaihua City,leading to mortality rate of 5. 3%. Through survey of incidence,combined with clinical symptoms,pathological changes and l...In November 2012,diarrhea-predominant disease broke out in a large rabbit farm in Huaihua City,leading to mortality rate of 5. 3%. Through survey of incidence,combined with clinical symptoms,pathological changes and laboratory diagnosis,the disease was confirmed as secondary infection of E. coli with rabbit coccidiosis,and it had been controlled after drug treatment.展开更多
In this paper, a pricing problem of European call options is considered, wbete the underlying stock generates dividends d, at some fixed future dates T, before the expiration date T .without the inappropriate assumpti...In this paper, a pricing problem of European call options is considered, wbete the underlying stock generates dividends d, at some fixed future dates T, before the expiration date T .without the inappropriate assumption made in that the dlvkdeMs being payed continously.The arbitrage free pricing of the option is determined via a series of partial differential equations.which is derived at the view point of backward s'tochasric differential ertuation (BBDE). It isshowed how the dividends affect the fair price of the call options. Some simulating results are alsogiven to illust rate the respective in fluence of parameters a.T.r,K.di and F1 on the option pricing.展开更多
The Newcastle disease is an acute infectious disease,which spreads rapidly and causes serious damage to the chicken industry.In recent years,due to various reasons,atypical,chronic Newcastle disease has appeared in se...The Newcastle disease is an acute infectious disease,which spreads rapidly and causes serious damage to the chicken industry.In recent years,due to various reasons,atypical,chronic Newcastle disease has appeared in several chicken farms,especially in the process of stocking in mountain forests.In this paper,the author analyzed the causes of atypical Newcastle disease occurred in mountain forest stocking chickens from a farm in Zhongfang County,Huaihua City,and proposed some control measures.展开更多
文摘The concept of finite-time stability for linear singular system is induced in this paper.Finite-time control problem is considered for linear singular systems with time-varying parametricuncertainties and exogenous disturbances. The disturbance satisfies a dynamical system with para-metric uncertainties. A su?cient condition is presented for robust finite-time stabilization via statefeedback. The condition is translated to a feasibility problem involving restricted linear matrix in-equalities (LMIs). A detailed solving method is proposed for the restricted linear matrix inequalities.Finally, an example is given to show the validity of the results.
基金This work is supported by the National Natural Science Foundation (Grant No.10371067)the Youth Teacher Foundation of Fok Ying Tung Education Foundation, the Excellent Young Teachers Program and the Doctoral Program Foundation of MOE and Shandong Province, China.
文摘In this paper, we use the solutions of forward-backward stochastic differential equations to get the explicit form of the optimal control for linear quadratic stochastic optimal control problem and the open-loop Nash equilibrium point for nonzero sum differential games problem. We also discuss the solvability of the generalized Riccati equation system and give the linear feedback regulator for the optimal control problem using the solution of this kind of Riccati equation system.
文摘Background The role of extracellular signal-regulated kinase 1/2 (ERK1/2) in shikonin-induced HeLa cells apoptosis remains vague. This study was to investigate the activation of caspase pathways and the role of ERK1/2 in human cervical cancer cells, HeLa, by shikonin. Methods The inhibitory effect of shikonin on the growth of HeLa cells was measured by MTT assay. Fluorescent microscopic analysis of apoptotic cells stained with 4’,6’-oliiamiclino-2-phenylindole C (DAPI) and Hoechst 33258 was carried out. Caspase-3 and -8 activities were detected using caspase-3 substrate and caspase-8 substrate as substrates, respectively. The protein levels of ERK, p53 and p-ERK were determined by Western blot analysis.Results Shikonin inhibited cell growth in a time- and dose-dependent manner. Caspase-3 and caspase-8 were activated in the apoptotic process and caspase inhibitors effectively reversed shikonin-induced apoptosis. Phosphorylation of ERK resulted in up-regulation of p53 expression, which was blocked by mitogen-activated protein kinase (MEK), inhibitor PD 98059.Conclusion Shikonin induces HeLa cell apoptosis through the ERK, p53 and caspase pathways.
基金funded by National Natural Science Foundation of China (No. 32071899)Walmart Foundation (No. UA2020– 154)。
文摘creening of foodborne pathogens is important to prevent contaminated foods from their supply chains.n this study, a portable detection device was developed for rapid, sensitive and simple detection of viable almonella using a finger-actuated microfluidic chip and an improved recombinase aided amplification (RAA) assay. Improved propidium monoazide(PMAxx) was combined with RAA to enable this device to distinguish viable bacteria from dead ones. The modification of PMAxx into dead bacteria, the magnetic xtraction of nucleic acids from viable bacteria and the RAA detection of extracted nucleic acids were performed using the microfluidic chip on its supporting device by finger press-release operations. The fluorescent signal resulting from RAA amplification of the nucleic acids was collected using a USB camera nd analyzed using a self-developed smartphone App to quantitatively determine the bacterial concenration. This device could detect Salmonella typhimurium in spiked chicken meats from 1.3 × 10^(2) CFU/m L o 1.3 × 10^(7) CFU/m L in 2 h with a lower detection limit of 130 CFU/m L, and has shown its potential for on-site detection of foodborne pathogens.
基金Supported by the National Natural Science Foundation of China under Grant(62301330,62101346)the Guangdong Basic and Applied Basic Research Foundation(2024A1515010496,2022A1515110101)+1 种基金the Stable Support Plan for Shenzhen Higher Education Institutions(20231121103807001)the Guangdong Provincial Key Laboratory under(2023B1212060076).
文摘Background Co-salient object detection(Co-SOD)aims to identify and segment commonly salient objects in a set of related images.However,most current Co-SOD methods encounter issues with the inclusion of irrelevant information in the co-representation.These issues hamper their ability to locate co-salient objects and significantly restrict the accuracy of detection.Methods To address this issue,this study introduces a novel Co-SOD method with iterative purification and predictive optimization(IPPO)comprising a common salient purification module(CSPM),predictive optimizing module(POM),and diminishing mixed enhancement block(DMEB).Results These components are designed to explore noise-free joint representations,assist the model in enhancing the quality of the final prediction results,and significantly improve the performance of the Co-SOD algorithm.Furthermore,through a comprehensive evaluation of IPPO and state-of-the-art algorithms focusing on the roles of CSPM,POM,and DMEB,our experiments confirmed that these components are pivotal in enhancing the performance of the model,substantiating the significant advancements of our method over existing benchmarks.Experiments on several challenging benchmark co-saliency datasets demonstrate that the proposed IPPO achieves state-of-the-art performance.
基金supported by the Natural Science Foundation of China (31701055 to Y.Wang)the Research Grants Council of Hong Kong (grant nos. HKBU12159916, HKBU262213, and AoE/M-403/16 to Y.X.)。
文摘Pattern-triggered immunity(PTI) is an essential strategy used by plants to deploy broad-spectrum resistance against pathogen attacks. Heterotrimeric G proteins have been reported to contribute to PTI.Of the three non-canonical EXTRA-LARGE G PROTEINs(XLGs) in Arabidopsis thaliana, XLG2 and XLG3 were shown to positively regulate immunity,but XLG1 was not considered to function in defense,based on the analysis of a weak xlg1 allele.In this study, we characterized the xlg1 xlg2 xlg3 triple knockout mutants generated from an xlg1 knockout allele. The strong xlg1 xlg2 xlg3 triple mutants compromised pathogen-associated molecular pattern(PAMP)-triggered activation of mitogen-activated protein kinases(MAPKs) and resistance to pathogen infection. The three XLGs interacted with MAPK cascade proteins involved in defense signaling, including the MAPK kinase kinases MAPKKK3 and MAPKKK5, the MAPK kinases MKK4 and MKK5, and the MAPKs MPK3 and MPK6. Expressing a constitutively active form of MKK4 restored MAPK activation and partially recovered the compromised disease resistance seen in the strong xlg1 xlg2 xlg3 triple mutant. Furthermore, mutations of all three XLGs largely restored the phenotype of the autoimmunity mutant bak1-interacting receptor-like kinase 1. Our study reveals that all three XLGs function redundantly in PAMP-triggered MAPK activation and plant immunity.
基金supported by National Natural Science Foundation of China (No. 32071899)Walmart Foundation (No.61626817)Walmart Food Safety Collaboration Center for its great support。
文摘Foodborne pathogenic bacteria have been considered as a major risk factor for food safety. It is of great significance to carry out in-field screening of pathogenic bacteria to prevent the outbreaks of foodborne diseases. In this study, a portable lab-on-a-disc platform with a microfluidic disc was developed for rapid and automatic detection of Salmonella typhimurium using a nickel nanowire(Ni NW) net for effective separation of target bacteria, horseradish peroxidase nanoflowers(HRP NFs) for efficient amplification of biological signals, and a self-developed smartphone APP for accurate analysis of colorimetric images. First,the microfluidic disc was preloaded with reagents and samples and centrifuged to form one bacterial sample column, one immune Ni NW column, one HRP NF column, two washing buffer columns and one tetramethylbenzidine(TMB) column, which were separated by air gaps. Then, a rotatable magnetic field was specifically developed to assemble the Ni NWs into a net, which was automatically controlled by a stepped motor to successively pass through the sample column for specific capture of target bacteria, the HRP NF column for specific label of target bacteria, the washing columns for effective removal of sample background and non-specific binding NFs, and the TMB column for colorimetric determination of target bacteria. The color change of TMB from colorless to blue was finally analyzed using the smartphone APP to quantitatively determine the target bacteria. This lab-on-a-disc platform could detect Salmonella typhimurium from 5.6 × 10^(1) CFU/20 μL to 5.6 × 10^(5) CFU/20 μL in 1 h with a lower detection limit of 56 CFU/20 μL. The recovery of target bacteria in spiked chicken samples ranged from 97.5% to 101.8%. This portable platform integrating separation, labeling, washing, catalysis and detection onto a single disc is featured with automatic operation, fast reaction, and small size and has shown its potential for in-field detection of foodborne pathogens.
基金supported by the Science and Technology Planning Project of Guangdong Province (grant numbers2016A020215160)the Medical Scientific Research foundation of Guangdong Province (grant numbers A2015603)
文摘An LCC delivery system for Fenofibrate (Fen) was developed to improve its poorly oral bioavailability. Fen-LCC preparation methods were screened, and the prepared Fen-LCC was then characterized by a polarizing microscope and transmission electron microscopy (TEM).The spray drying technique was selected to dry and solidify particles into powder. The in vitro release of Fen-LCC was measured and in vivo pharmacokinetic experiments were carried out on rats after oral administration. Particles prepared through the high-temperature input method exhibited structural characteristics of LCC, and re-dissolved particles maintained the same features. The LCC delivery system can significantly improve in vitro release outcomes. After oral administration, AUCs of the suspension and LCC systems were measured at 131.6853 μg·h/ml and 1435.72893 μg·h/ml, respectively. The spray drying process presented here better maintains cubic structures, and the LCC system significantly improves bioavailability levels.
文摘In November 2012,diarrhea-predominant disease broke out in a large rabbit farm in Huaihua City,leading to mortality rate of 5. 3%. Through survey of incidence,combined with clinical symptoms,pathological changes and laboratory diagnosis,the disease was confirmed as secondary infection of E. coli with rabbit coccidiosis,and it had been controlled after drug treatment.
文摘In this paper, a pricing problem of European call options is considered, wbete the underlying stock generates dividends d, at some fixed future dates T, before the expiration date T .without the inappropriate assumption made in that the dlvkdeMs being payed continously.The arbitrage free pricing of the option is determined via a series of partial differential equations.which is derived at the view point of backward s'tochasric differential ertuation (BBDE). It isshowed how the dividends affect the fair price of the call options. Some simulating results are alsogiven to illust rate the respective in fluence of parameters a.T.r,K.di and F1 on the option pricing.
文摘The Newcastle disease is an acute infectious disease,which spreads rapidly and causes serious damage to the chicken industry.In recent years,due to various reasons,atypical,chronic Newcastle disease has appeared in several chicken farms,especially in the process of stocking in mountain forests.In this paper,the author analyzed the causes of atypical Newcastle disease occurred in mountain forest stocking chickens from a farm in Zhongfang County,Huaihua City,and proposed some control measures.
