为更好地应用重复脉冲(RSO)法进行发电机转子匝间绝缘诊断,对匝间短路故障下重复脉冲的响应特性、故障定位算法及严重程度分析展开研究。首先,基于改进矢量拟合算法,在确定绕组的梯形等效电路拓扑以及单位长度等效电路阶数后,采用电路...为更好地应用重复脉冲(RSO)法进行发电机转子匝间绝缘诊断,对匝间短路故障下重复脉冲的响应特性、故障定位算法及严重程度分析展开研究。首先,基于改进矢量拟合算法,在确定绕组的梯形等效电路拓扑以及单位长度等效电路阶数后,采用电路仿真软件仿真重复脉冲在发电机转子绕组中的时域响应特性。随后,基于仿真模型,设置多种不同位置、不同严重程度的故障,采用多信号分类法(multiple signal classification,MUSIC)算法估计重复脉冲注入信号与特征曲线的起始位置,通过时间差以及脉冲传播速度定位故障源。最后,通过改变故障位置、匝间短路长度与参考端,研究了故障严重程度的变化趋势。研究结果表明:改进的矢量拟合算法具有运算效率高、现场使用方便的优点;应用MUSIC算法的定位误差不超过1.83%,能够满足现场检测要求;特征波形畸变面积能够用于表征故障严重程度且具有更高的可靠性。研究结果证明了所提方法的有效性。展开更多
Surface ozone(O_(3))is a major air pollutant and draw increasing attention in the Pearl River Delta(PRD),China.Here,we characterize the spatial-temporal variability of ozone based on a dataset obtained from 57 nationa...Surface ozone(O_(3))is a major air pollutant and draw increasing attention in the Pearl River Delta(PRD),China.Here,we characterize the spatial-temporal variability of ozone based on a dataset obtained from 57 national monitoring sites during 2013-2019.Our results show that:(1)the seasonal difference of ozone distribution in the inland and coastal areas was significant,which was largely affected by the wind pattern reversals related to the East Asian monsoon,and local ozone production and destruction;(2)the daily maximum 8hr average(MDA8 O_(3))showed an overall upward trend by 1.11 ppbv/year.While the trends in the nine cities varied differently by ranging from-0.12 to 2.51 ppbv/year.The hot spots of ozone were spreading to southwestern areas from the central areas since 2016.And ozone is becoming a year-round air pollution problem with the pollution season extending to winter and spring in PRD region.(3)at the central and southwestern PRD cities,the percentage of exceedance days from the continuous type(defined as≥3 days)was increasing.Furthermore,the ozone concentration of continuous type was much higher than that of scattered exceedance type(<3 days).In addition,although the occurrence of continuous type starts to decline since2017,the total number of exceedance days during the continuous type is increasing.These results indicate that it is more difficult to eliminate the continuous exceedance than the scatter pollution days and highlight the great challenge in mitigation of O_(3)pollution in these cities.展开更多
Silicosis is a leading cause of occupational disease-related morbidity and mortality worldwide,but the molecular basis underlying its development remains unclear.An accumulating body of evidence supports gasdermin D(G...Silicosis is a leading cause of occupational disease-related morbidity and mortality worldwide,but the molecular basis underlying its development remains unclear.An accumulating body of evidence supports gasdermin D(GSDMD)-mediated pyroptosis as a key component in the development of various pulmonary diseases.However,there is little experimental evidence connecting silicosis and GSDMD-driven pyroptosis.In this work,we investigated the role of GSDMD-mediated pyroptosis in silicosis.Single-cell RNA sequencing of healthy and silicosis human and murine lung tissues indicated that GSDMD-induced pyroptosis in macrophages was relevant to silicosis progression.Through microscopy we then observed morphological alterations of pyroptosis in macrophages treated with silica.Measurement of interleukin-1βrelease,lactic dehydrogenase activity,and real-time propidium iodide staining further revealed that silica induced pyroptosis of macrophages.Additionally,we verified that both canonical(caspase-1-mediated)and non-canonical(caspase-4/5/11-mediated)signaling pathways mediated silica-induced pyroptosis activation,in vivo and in vitro.Notably,Gsdmd knockout mice exhibited dramatically alleviated silicosis phenotypes,which highlighted the pivotal role of pyroptosis in this disease.Taken together,our results demonstrated that macrophages underwent GSDMD-dependent pyroptosis in silicosis and inhibition of this process could serve as a viable clinical strategy for mitigating silicosis.展开更多
目的:构建α7烟碱型乙酰胆碱受体(α7 nicotine acetylcholine receptor,α7 n AChR)基因敲除小鼠牙髓炎模型,为研究α7 nAChR在牙髓炎发生发展过程中的作用机制提供实验模型。方法:取16只α7 n AChR基因敲除(knock out,KO)小鼠和16只...目的:构建α7烟碱型乙酰胆碱受体(α7 nicotine acetylcholine receptor,α7 n AChR)基因敲除小鼠牙髓炎模型,为研究α7 nAChR在牙髓炎发生发展过程中的作用机制提供实验模型。方法:取16只α7 n AChR基因敲除(knock out,KO)小鼠和16只野生型(wide type,WT)C57BL/6鼠的上颌第一磨牙的牙髓暴露法建立牙髓炎模型,分别在牙髓暴露后1、3、7 d处死小鼠,HE染色、免疫组化检测牙髓组织NOD样受体蛋白3(NOD-like receptor protein 3,NLRP3)表达情况。结果:HE染色结果显示牙髓暴露后1 d,α7 n AChR KO鼠及WT鼠的穿髓孔周围血管充血、组织水肿;牙髓暴露后3 d,炎症细胞聚集,α7 n AChR KO鼠炎症细胞聚集已达冠髓底部,WT鼠的炎症细胞主要聚集在穿髓孔周围的牙髓组织中;牙髓暴露后7 d,α7 nAChR KO鼠的牙髓炎症细胞浸润至根部牙髓,而WT鼠的牙髓炎症细胞仍然主要聚集于冠髓底部。免疫组化染色结果显示,牙髓暴露后,α7 n AChR KO鼠牙髓NLRP3表达高于WT鼠,差异有统计学意义(P <0.05)。结论:成功建立了α7 n AChR基因敲除鼠的牙髓炎模型,而且α7nAChR基因敲除鼠牙髓炎症浸润明显快于WT小鼠。展开更多
文摘为更好地应用重复脉冲(RSO)法进行发电机转子匝间绝缘诊断,对匝间短路故障下重复脉冲的响应特性、故障定位算法及严重程度分析展开研究。首先,基于改进矢量拟合算法,在确定绕组的梯形等效电路拓扑以及单位长度等效电路阶数后,采用电路仿真软件仿真重复脉冲在发电机转子绕组中的时域响应特性。随后,基于仿真模型,设置多种不同位置、不同严重程度的故障,采用多信号分类法(multiple signal classification,MUSIC)算法估计重复脉冲注入信号与特征曲线的起始位置,通过时间差以及脉冲传播速度定位故障源。最后,通过改变故障位置、匝间短路长度与参考端,研究了故障严重程度的变化趋势。研究结果表明:改进的矢量拟合算法具有运算效率高、现场使用方便的优点;应用MUSIC算法的定位误差不超过1.83%,能够满足现场检测要求;特征波形畸变面积能够用于表征故障严重程度且具有更高的可靠性。研究结果证明了所提方法的有效性。
基金supported by the Guangdong Major Project of Basic and Applied Basic Research (No.2020B0301030004)the National Natural Science Foundation of China (No.42175111)+1 种基金the Guangdong science and technology plan project (No.2019B121201002)the Fundamental Research Funds for the Central Universities,Sun Yat-sen University (No.22qntd1908)。
文摘Surface ozone(O_(3))is a major air pollutant and draw increasing attention in the Pearl River Delta(PRD),China.Here,we characterize the spatial-temporal variability of ozone based on a dataset obtained from 57 national monitoring sites during 2013-2019.Our results show that:(1)the seasonal difference of ozone distribution in the inland and coastal areas was significant,which was largely affected by the wind pattern reversals related to the East Asian monsoon,and local ozone production and destruction;(2)the daily maximum 8hr average(MDA8 O_(3))showed an overall upward trend by 1.11 ppbv/year.While the trends in the nine cities varied differently by ranging from-0.12 to 2.51 ppbv/year.The hot spots of ozone were spreading to southwestern areas from the central areas since 2016.And ozone is becoming a year-round air pollution problem with the pollution season extending to winter and spring in PRD region.(3)at the central and southwestern PRD cities,the percentage of exceedance days from the continuous type(defined as≥3 days)was increasing.Furthermore,the ozone concentration of continuous type was much higher than that of scattered exceedance type(<3 days).In addition,although the occurrence of continuous type starts to decline since2017,the total number of exceedance days during the continuous type is increasing.These results indicate that it is more difficult to eliminate the continuous exceedance than the scatter pollution days and highlight the great challenge in mitigation of O_(3)pollution in these cities.
基金supported by Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(CIFMSNos.2021-1-I2M-049 and 2018-I2M-1-001,China)+1 种基金the Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences(Nos.2019RC330001 and 2021RC310002,China)National Natural Science Foundation of China(No.82090010)。
文摘Silicosis is a leading cause of occupational disease-related morbidity and mortality worldwide,but the molecular basis underlying its development remains unclear.An accumulating body of evidence supports gasdermin D(GSDMD)-mediated pyroptosis as a key component in the development of various pulmonary diseases.However,there is little experimental evidence connecting silicosis and GSDMD-driven pyroptosis.In this work,we investigated the role of GSDMD-mediated pyroptosis in silicosis.Single-cell RNA sequencing of healthy and silicosis human and murine lung tissues indicated that GSDMD-induced pyroptosis in macrophages was relevant to silicosis progression.Through microscopy we then observed morphological alterations of pyroptosis in macrophages treated with silica.Measurement of interleukin-1βrelease,lactic dehydrogenase activity,and real-time propidium iodide staining further revealed that silica induced pyroptosis of macrophages.Additionally,we verified that both canonical(caspase-1-mediated)and non-canonical(caspase-4/5/11-mediated)signaling pathways mediated silica-induced pyroptosis activation,in vivo and in vitro.Notably,Gsdmd knockout mice exhibited dramatically alleviated silicosis phenotypes,which highlighted the pivotal role of pyroptosis in this disease.Taken together,our results demonstrated that macrophages underwent GSDMD-dependent pyroptosis in silicosis and inhibition of this process could serve as a viable clinical strategy for mitigating silicosis.
基金supported by National Nature Science Foundation for Excellent Innovation Research Group of China (71021061)National Nature Science Foundation of China (70701008, 70871021, 90924016 and71171043)Humanities and Society Science Plan Foundation of Ministry of Education of China (11YJA630180)
文摘目的:构建α7烟碱型乙酰胆碱受体(α7 nicotine acetylcholine receptor,α7 n AChR)基因敲除小鼠牙髓炎模型,为研究α7 nAChR在牙髓炎发生发展过程中的作用机制提供实验模型。方法:取16只α7 n AChR基因敲除(knock out,KO)小鼠和16只野生型(wide type,WT)C57BL/6鼠的上颌第一磨牙的牙髓暴露法建立牙髓炎模型,分别在牙髓暴露后1、3、7 d处死小鼠,HE染色、免疫组化检测牙髓组织NOD样受体蛋白3(NOD-like receptor protein 3,NLRP3)表达情况。结果:HE染色结果显示牙髓暴露后1 d,α7 n AChR KO鼠及WT鼠的穿髓孔周围血管充血、组织水肿;牙髓暴露后3 d,炎症细胞聚集,α7 n AChR KO鼠炎症细胞聚集已达冠髓底部,WT鼠的炎症细胞主要聚集在穿髓孔周围的牙髓组织中;牙髓暴露后7 d,α7 nAChR KO鼠的牙髓炎症细胞浸润至根部牙髓,而WT鼠的牙髓炎症细胞仍然主要聚集于冠髓底部。免疫组化染色结果显示,牙髓暴露后,α7 n AChR KO鼠牙髓NLRP3表达高于WT鼠,差异有统计学意义(P <0.05)。结论:成功建立了α7 n AChR基因敲除鼠的牙髓炎模型,而且α7nAChR基因敲除鼠牙髓炎症浸润明显快于WT小鼠。
