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Analysis of Chemical and Biological Soil Properties in Organically and Conventionally Fertilized Apple Orchards 被引量:6
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作者 takamitsu kai Masaki Mukai +2 位作者 Kiwako S. Araki Dinesh Adhikari Motoki Kubo 《Journal of Agricultural Chemistry and Environment》 2016年第2期92-99,共8页
We compared chemical and biological properties of soils in organically and conventionally fertilized apple orchards in Nagano Prefecture (one of the major apple producing regions in Japan). Five apple orchards with di... We compared chemical and biological properties of soils in organically and conventionally fertilized apple orchards in Nagano Prefecture (one of the major apple producing regions in Japan). Five apple orchards with different fertilizer management systems were used for this study. The total carbon and total nitrogen contents were higher in the organically fertilized orchard, while the total phosphorus and total potassium were at similar levels in both organically and conventionally fertilized orchards. The bacterial biomass did not differ between the two orchards, but the N circulation activity was clearly higher in the organically fertilized orchard from April to December. Total carbon from 50,000 to 60,000 mg/kg, total nitrogen at about 3000 to 4000 mg/kg, and a C/N ratio of 15 - 20 were suggested to be suitable conditions for a high level of apple production under an organic fertilizer management system. 展开更多
关键词 Component Formatting Style STYLING INSERT Organic Fertilizer Management System APPLE Bacterial Biomass Nitrogen Circulation Activity
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Degradation of Bioplastics in Soil and Their Degradation Effects on Environmental Microorganisms 被引量:4
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作者 Dinesh Adhikari Masaki Mukai +4 位作者 Kenzo Kubota takamitsu kai Nobuyuki Kaneko Kiwako S. Araki Motoki Kubo 《Journal of Agricultural Chemistry and Environment》 2016年第1期23-34,共12页
Degradation of three kinds of bioplastics and their effects on microbial biomass and microbial diversity in soil environment were analyzed. The degradation rate of bioplastic in soil was closely related to the main co... Degradation of three kinds of bioplastics and their effects on microbial biomass and microbial diversity in soil environment were analyzed. The degradation rate of bioplastic in soil was closely related to the main components in the bioplastics. Poly (butylene succinate)-starch (PBS-starch) and poly (butylene succinate) (PBS) were degraded by 1% to 7% after 28 days in a soil with an initial bacterial biomass of 1.4 × 10<sup>9</sup> cells/g-soil, however poly lactic acid (PLA) was not degraded in the soil after 28 days. When the powdered-bioplastics were examined for the degradation in the soil, PBS-starch also showed the highest degradability (24.4% degradation after 28 days), and the similar results were obtained in the case of long-term degradation experiment (2 years). To investigate the effect of bacterial biomass in soil on biodegradability of bioplastics, PBS-starch was buried in three kinds of soils differing in bacterial biomass (7.5 × 10<sup>6</sup>, 7.5 × 10<sup>7</sup>, and 7.5 × 10<sup>8</sup> cells/g-soil). The rate of bioplastic degradation was enhanced accompanied with an increase of the bacterial biomass in soil. 16S rDNA PCR-DGGE analysis indicated that the bacterial diversity in the soil was not affected by the degradation of bioplastics. Moreover, the degradation of bioplastic did not affect the nitrogen circulation activity in the soil. 展开更多
关键词 BIOPLASTICS BIODEGRADATION Soil Microorganisms Bacterial Diversity
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A Study on Rice Growth and Soil Environments in Paddy Fields Using Different Organic and Chemical Fertilizers 被引量:3
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作者 takamitsu kai Motoki Kumano Masahiko Tamaki 《Journal of Agricultural Chemistry and Environment》 2020年第4期331-342,共12页
Currently, the majority of paddy fields in Japan are grown using chemical fertilizers and synthetic chemical pesticides, since chemical fertilizers can provide the nutrients necessary for plant growth. However, there ... Currently, the majority of paddy fields in Japan are grown using chemical fertilizers and synthetic chemical pesticides, since chemical fertilizers can provide the nutrients necessary for plant growth. However, there are concerns regarding the environmental impact of chemical fertilizer and pesticides production, such as reduction of soil microorganisms and water pollution due to the runoff of fertilizer components from the soil caused by excessive fertilizer application. In this study, we investigated the effects of the application of organic and chemical fertilizers on the plant growth of paddy fields, in addition to their effects on the chemical and biological properties of the soil. The panicle numbers of rough and brown rice, the 1000-grain weight of the rough and brown rice, and the percentages of ripened grains were significantly higher in paddy soils grown with organic fertilizers than in those grown with chemical fertilizers. In addition, the total carbon (TC) contents and pH values were significantly higher in the soils of paddy fields grown with organic fertilizers. Furthermore, the soils of paddy fields grown with organic fertilizers exhibited greater bacterial biomasses, N circulation activity, and P circulation activity than the soils of paddy fields grown using chemical fertilizers, although the differences were not significant. In this study, the difference in plant growth <span>was </span><span>appeared in fertilizer application such as organic and chemical fertilizers. It was indicated that the organic fertilizer and pesticide reduction management increased the soil bacterial biomass and activated the material cycle such as N circulation activity.</span> 展开更多
关键词 RICE Organic Fertilizer Soil Microorganism Soil Fertility Agricultural Environment Environmental Conservation
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Effect of Organic and Chemical Fertilizer Application on Growth, Yield, and Soil Biochemical Properties of Landrace <i>Brassica napus</i>L. Leaf-and-Stem Vegetable and Landrace (Norabona) 被引量:1
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作者 takamitsu kai Masahiko Tamaki 《Journal of Agricultural Chemistry and Environment》 2020年第4期314-330,共17页
<span style="font-family:Verdana;">Norabona </span><span style="font-family:Verdana;">i</span><span style="font-family:Verdana;">s generally cultivated in Ja... <span style="font-family:Verdana;">Norabona </span><span style="font-family:Verdana;">i</span><span style="font-family:Verdana;">s generally cultivated in Japan under management systems that use chemical fertilizers and synthetic chemical pesticides. However, the continuous use of these fertilizers and pesticides damages the soil environment and reduces the number of soil microorganisms. There has been little research investigating the effect of organic and chemical fertilizer applications on soil biochemistry and the growth and yield of norabona. In this study, we investigated the effect of organic and chemical fertilizer application on these factors during the norabona growing season from September 2019 to May 2020.</span><span style="font-family:Verdana;"> </span><span style="font-family:Verdana;">Leaf length, shoot height, and shoot width were significantly higher under organic fertilizer management in the early stage of cultivation (in March) than under chemical fertilizer management. However, there was no significant difference between treatments for these growth parameters in later months, nor for any other parameters. Soil TN, and TP contents were significantly higher in the organic fertilizer treatment after harvest than prior to cultivation or after the chemical fertilizer treatment. In addition, soil TC, and volumetric water content were significantly higher in the organic fertilizer treatment than in chemical fertilizer treatment. The higher TC, TN, and C/N ratio in organic fertilizer treated soil appeared to increase the bacterial biomass, leading to enhanced nutrient circulation via N and P circulation activity, producing a rich soil environment with active soil microorganisms.</span> 展开更多
关键词 Organic Fertilizer Soil Microorganisms Soil Fertility Agricultural Environment Environmental Conservation
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Effect of Organic and Chemical Fertilizer Application on Growth, Yield, and Quality of Small-Sized Tomatoes 被引量:1
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作者 takamitsu kai Shunya Nishimori Masahiko Tamaki 《Journal of Agricultural Chemistry and Environment》 2020年第3期121-133,共13页
Tomatoes in Japan are generally cultivated under management systems that use chemical fertilizers and synthetic chemical pesticides. However, the continuous use of these fertilizers and pesticides damages the soil env... Tomatoes in Japan are generally cultivated under management systems that use chemical fertilizers and synthetic chemical pesticides. However, the continuous use of these fertilizers and pesticides damages the soil environment and reduces the number of soil microorganisms. Organic farming has a relatively low environmental impact compared to conventional farming techniques, but typically has lower and more unstable yields. In this study, we investigated the effect of organic and chemical fertilizer application on growth, yield, and quality of small-sized (cherry) tomatoes. Cherry tomatoes were cultivated using organic and chemical organic fertilizers. Average weight and lateral diameter were significantly higher under organic fertilizer than under chemical fertilizer. In addition, shoot dry weight was significantly higher under organic fertilizer than chemical fertilizer. Lycopene content was significantly higher under organic fertilizer than chemical fertilizer. The total carbon (TC), total phosphorus (TP), total potassium (TK), available phosphoric (SP) and exchangeable potassium (SK) contents, C/N ratio, and pH were significantly higher under organic fertilizer than chemical fertilizer. Bacterial biomass, nitrite (NO</span><span style="font-family:Verdana;">? 2</span><span style="font-family:Verdana;">-N) oxidation activity, nitrification (N) circulation activity, and phosphoric (P) circulation were higher under organic fertilizer than chemical fertilizer. From these results, the study indicates that appropriate controls such as TC, total nitrogen (TN), and C/N ratio of organic fertilizer increased microbial biomass and enhanced nutrient circulation such as N circulation activity and P circulation activity. </span><span style="font-family:Verdana;">These results can be used to improve current organic farming practices and promote soil conservation. 展开更多
关键词 Small-Sized Tomato Organic Fertilizer Soil Microorganisms Soil Fertility Agricultural Environment Environmental Conservation
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Effects of 5-Aminolevulinic Acid (ALA) on Zinnia hybrida Growth and Phytoremediation Effects in Oil-Contaminated Soil
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作者 takamitsu kai Hiromi Ikeura +1 位作者 Ariaki Ayanokoji Masahiko Tamaki 《Journal of Agricultural Chemistry and Environment》 2020年第3期97-106,共10页
In this study, we compared plant height, weight, soil TPH concentration, and soil DHA level after 18 weeks of </span><i><span style="font-family:Verdana;">Zennia</span></i><s... In this study, we compared plant height, weight, soil TPH concentration, and soil DHA level after 18 weeks of </span><i><span style="font-family:Verdana;">Zennia</span></i><span style="font-family:Verdana;"> <i>hybrida</i> </span><span style="font-family:Verdana;">cultivation with four different concentrations of 5-Aminolevulinic acid (ALA)-based liquid fertilizer: 1500-fold, 5000-fold, and 8000-fold dilutions, along with a non-treatment control of diluted ALA. The plants of ALA-treated were significantly taller than the non-treatment control. The plants of ALA-treated plants were higher in shoot fresh weight, shoot dry weight, and root dry weight than the non-treatment control. The plot of ALA-based liquid fertilizer with the 5000-fold dilution was significantly highest in shoot fresh weight, shoot dry weight, and root dry weight. ALA-treated plants were lower in the soil Total Petroleum </span></span><span style="font-family:Verdana;">Hydrocarbon </span><span style="font-family:""><span style="font-family:Verdana;">(TPH) concentration than the non-treatment control. The plot of ALA-based liquid fertilizer with the 5000-fold was significantly lowest in the TPH concentration. In addition, ALA-treated plants were </span><span style="font-family:Verdana;">higher in the soil dehydrogenase activity (DHA) than the non-treatment control. The plot of ALA-based liquid fertilizer with the 5000-fold was signifi</span><span style="font-family:Verdana;">cantly highest in the TPH concentration. This study indicated that ALA-applied zinnia-grown oil-contaminated soil is more effective than not. The remediation in oil-contaminated soil with ALA-based liquid fertilizer is more effective than the non-treatment control</span></span><span style="font-family:Verdana;">;</span><span style="font-family:Verdana;">furthermore, ALA application with 5000-fold dilution was most suitable in oil-contaminated soil among other plots. 展开更多
关键词 PHYTOREMEDIATION ZINNIA 5-Aminolevulinic Acid Soil Total Petroleum Hydrocarbon (TPH) Concentration Soil Dehydrogenase Activity (DHA)
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Phytoremediation of Oil-Contaminated Soils by Combining Flowering Plant Cultivation and Inoculation with <i>Acinetobacter junii</i>Strain M-2
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作者 takamitsu kai Yu Okamoto +1 位作者 Shuichiro Murakami Masahiko Tamaki 《Journal of Agricultural Chemistry and Environment》 2020年第3期107-120,共14页
Oil contamination of the soil by petroleum products has become an enormous environmental problem. In this study, we examined whether remediation of oil-contaminated soils by cultivating three flowering plants (Mimosa,... Oil contamination of the soil by petroleum products has become an enormous environmental problem. In this study, we examined whether remediation of oil-contaminated soils by cultivating three flowering plants (Mimosa, Gazania, and Zinnia) could be enhanced by inoculation with </span><i><span style="font-family:Verdana;">Acinetobacter junii</span></i><span style="font-family:Verdana;"> strain M-2 at different plant growth stages (at sowing, at early growth, and at mid-growth). The growth of Zinnia cultivated in oil-contaminated soils inoculated at sowing was significantly superior to that in the non-inoculated soil. Although total petroleum hydrocarbon concentrations in soils inoculated at sowing were nominally lower than those in non-inoculated soils, especially in the case of Zinnia planting, the effect did not reach statistical significance. However, dehydrogenase activity was significantly higher in </span><span><span style="font-family:Verdana;">the soils inoculated with </span><i><span style="font-family:Verdana;">A. junii</span></i><span style="font-family:Verdana;"> strain M-2 than in non-inoculated soils for all three plant species tested. These results demonstrate that a combination of ornamental plant cultivation (particularly Zinnia) and inoculation with </span><i><span style="font-family:Verdana;">A. junii</span></i><span style="font-family:Verdana;"> strain M-2 increases the efficiency of oil-contaminated soil phytoremediation. 展开更多
关键词 PHYTOREMEDIATION ZINNIA Acinetobacter junii Strain M-2 Total Petroleum Hydrocarbon Dehydrogenase Activity
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Chemical and Biological Properties of Apple Orchard Soils under Natural, Organic, Hybrid, and Conventional Farming Methods
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作者 takamitsu kai Motoki Kubo 《Journal of Agricultural Chemistry and Environment》 2020年第3期134-146,共13页
Apples in Japan are generally cultivated under management systems that use chemical fertilizers and synthetic chemical pesticides. However, the continuous use of these fertilizers and pesticides damages the soil envir... Apples in Japan are generally cultivated under management systems that use chemical fertilizers and synthetic chemical pesticides. However, the continuous use of these fertilizers and pesticides damages the soil environment and reduces the number of soil microorganisms. In this study, we compared the chemical and biological properties of 12 soils from apple orchards in Aomori and Nagano Prefectures under four types of management systems, namely, natural conditions, with no cultivation, fertilizers, or pesticides;</span><a name="_Hlk37437201"></a><span style="font-size:12px;font-family:Verdana;">organic farming methods, using organic materials and pesticides approved </span><span style="font-size:12px;font-family:Verdana;">by the Japanese Agricultural Standard organic certification system;hybrid farming methods, using a mix of organic and chemical fertilizers;and conventional farming, using chemical fertilizers and pesticides. Soil total carbon (TC), total nitrogen (TN), total phosphorus (TP), nitrate-nitrogen (NO? 3), and available phosphoric acid (SP) contents were generally found to be the highest where organic farming methods were used. Similarly, bacterial biomass, nitrification (N) circulation activity, ammonia (NH+ 4) oxidation activity, nitrite (NO? 2) oxidation activity, and phosphoric (P) circulation activity were the highest under organic farming, especially in comparison with conventional farming. This study indicated that the differences in apple sugar content, acidity, and sugar/acidity ratio between different orchard management systems were due to different soil conditions, and soil conditions under organic farming management system in apple cultivation increased bacterial biomass while enhancing N and P circulation activity and high TC. On the other hand, the soil of conventional farming has the lowest total number of bacterial biomass and lowest material cycle such as N</span><span style="font-size:10pt;font-family:""> </span><span style="font-size:12px;font-family:Verdana;">and P circulation acti 展开更多
关键词 Organic Farming Soil Microorganisms Soil Fertility Agricultural Environment Environmental Conservation
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Characterization of Orchard Fields Based on Soil Fertility Index (SOFIX)
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作者 Pitchayapa Pholkaw Quoc Thinh Tran +4 位作者 takamitsu kai Taiki Kawagoe Kenzo Kubota Kiwako S. Araki Motoki Kubo 《Journal of Agricultural Chemistry and Environment》 2020年第3期159-176,共18页
Soil samples from 139 agricultural orchard fields (apple, grape, tea, and others) w</span><span style="font-family:Verdana;">ere</span><span style="font-family:Verdana;"> an... Soil samples from 139 agricultural orchard fields (apple, grape, tea, and others) w</span><span style="font-family:Verdana;">ere</span><span style="font-family:Verdana;"> analyzed using the soil fertility index. From these samples, an orchard field database was constructed and the soil properties between orchard, upland, and paddy fields </span><span style="font-family:Verdana;">were </span><span style="font-family:""><span style="font-family:Verdana;">compared. The average value of bacterial biomass in the orchard fields was 7.4 × 10</span><sup><span style="font-family:Verdana;">8</span></sup><span style="font-family:Verdana;"> cells/g-soil, ranging from not detected (lower than 6.6 × 10</span><sup><span style="font-family:Verdana;">6</span></sup><span style="font-family:Verdana;"> cells/g-soil) to 7.7 × 10</span><sup><span style="font-family:Verdana;">9</span></sup><span style="font-family:Verdana;"> cells/g-soil. The average values of total carbon (TC), total nitrogen (TN), total phosphorus (TP), and total potassium (TK), were 24,000 mg/kg (2670 to 128,100 mg/kg), 1460 mg/kg (133 to 6400 mg/kg), 1030 mg/kg (142 to 5362 mg/kg), and 5370 mg/kg (1214 to 18,155 mg/kg), respectively. The C/N and C/P ratios were 19 (3 to 85) and 27 (2 to 101), respectively. Soil properties of the orchard fields were compared with those of the upland and the paddy fields. The average value of bacterial biomass in the orchard fields was almost the same as that in the upland fields (8.0 × 10</span><sup><span style="font-family:Verdana;">8</span></sup><span style="font-family:Verdana;"> cells/g-soil), but the number was lower than that in the paddy fields (12.9 × 10</span><sup><span style="font-family:Verdana;">8</span></sup><span style="font-family:Verdana;"> cells/g-soil). The average values of TC and TN in the orchard fields fell between those in the upland fields (TC: 33,120 mg/kg, TN: 2010 mg/kg) and the paddy fields (TC: 15,420 mg/kg, TN: 1080</span></span><span style="font-family:""> </span><span style="font-family:Verdana;" 展开更多
关键词 Orchard Field Soil Fertility Index (SOFIX) Bacterial Biomass
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