Organomercury lyase (MerB) overexpressed in <em>Escherichia coli</em> captured and decomposed organomercury compounds, and it has been detected by radioactive analysis with neutron irradiation. Genetically...Organomercury lyase (MerB) overexpressed in <em>Escherichia coli</em> captured and decomposed organomercury compounds, and it has been detected by radioactive analysis with neutron irradiation. Genetically modified <em>E. coli</em> captures a lot of mercury from a cultivation solution with about 80% recovery, when the bacteria are growing during 24 to 72 hours. Since the modified <em>E. coli</em> has no additive gene for mercury metabolism, the bacteria could hold mercury tightly by the MerB enzyme in their cell and do not release them into medium. In the later, 72 hours after, bacteria have less recovery ratio;it may be affected by undecompsed mercury compounds in bacteria growth. The recovery ability of the bacteria would not be changed by addition of the MerB producing reagent (IPTG). A quantitative value of mercury atom is estimated by an emission of <em>γ</em>-ray by reactor neutron from a dried cell or solution on a filter paper, which is available for nondestructive testing of bacteria holding mercury atoms. In this method an efficient recovery system of toxic mercury from a polluted solution has been archived without destruction of samples, so called <em>in-cell</em> analysis.展开更多
Spontaneous transition of the plasma confinement mode was observed in thehelical-axis heliotron device 'Heliotron J' for three different plasma heating schemes, i.e.ECH-only, NBI-only and the combination of EC...Spontaneous transition of the plasma confinement mode was observed in thehelical-axis heliotron device 'Heliotron J' for three different plasma heating schemes, i.e.ECH-only, NBI-only and the combination of ECH and NBI. The transition seems to occur above a certaincritical density. In addition to the confinement transition, a spontaneous shift of the hittingposition of the divertor plasma flux on the wall was observed. This shift could be related with thechange of the edge field topology caused by non-inductive toroidal currents.展开更多
We report herein a rare case of lateral parapatellar synovial plica that developed simultaneously in both knees. A 15-year-old competitive soccer player visited our institution with a six-month history of anterolatera...We report herein a rare case of lateral parapatellar synovial plica that developed simultaneously in both knees. A 15-year-old competitive soccer player visited our institution with a six-month history of anterolateral pain and catching sensation in both knee joints. On physical examination, he complained of tenderness along the joint line of bilateral patellofemoral joint (PFJ). Visible and palpable popping was observed at the lateral margin of each patella during active knee motion. Magnetic resonance imaging showed obvious synovial soft nodules in the lateral side of the PFJ. On arthroscopy, a yellowish, thick, tongue-shaped tissue extending transversely from the lateral parapatellar synovium was identified in both knees, and parts of this tissue showed avascular hypertrophy. Arthroscopic findings of both knees are almost symmetrical in anatomical location, but no similar in size. After arthroscopic excision, the patient became asymptomatic. At 24-month follow up, he demonstrated full knee function, without evidence of local recurrence.展开更多
Organic soft linear actuators were fabricated using galvanostatic electropolymerization of the polypyrrole (PPy) thin film using a methyl benzoate electrolyte solution of N,N-Diethyl-N-methyl-N-(2-methoxyethyl) ammoni...Organic soft linear actuators were fabricated using galvanostatic electropolymerization of the polypyrrole (PPy) thin film using a methyl benzoate electrolyte solution of N,N-Diethyl-N-methyl-N-(2-methoxyethyl) ammonium bis (trifluoromethanesulfonyl) imide. The electrochemical deformation behaviors of the PPy actuators were investigated in aqueous solutions of an electrolyte, lithium bis (trifluoromethanesulphonyl) imide (LiTFSI) or sodium chloride (NaCl), containing different concentrations of methanol. The actuating strain of approximately 9% was achieved when the actuator was driven by a potential between –1 and 1 V with the potential sweep rate of 10 mV/s corresponding to 0.0025 Hz in the LiTFSI electrolyte containing 40% to 50% of methanol under a load stress of 0.3 MPa. However, the PPy actuator could not catch up with the higher frequency. On the other hand, the PPy actuator caught up with the potential sweep up to 0.1 Hz in the NaCl solutions with a methanol concentration between 40% and 60% with the expense of the actuating strain to approximately 1%.展开更多
Background:Metabolic syndrome(MetS) is a global health problem.Physical activity(PA) is a known modifiable risk factor for MetS and individual MetS components.However,the role of PA could differ between sub-population...Background:Metabolic syndrome(MetS) is a global health problem.Physical activity(PA) is a known modifiable risk factor for MetS and individual MetS components.However,the role of PA could differ between sub-populations due to differences in the variability of PA and other MetS risk factors.To examine these differences,multi-country studies with standardized outcome measurement methods across cohorts are needed.Methods:Cross-sectional PA levels(total and domain specific) in healthy middle-aged(44-56 years) men in the Risk Factor Assessment among Japanese and U.S.Men in the Post-World War Ⅱ Birth Cohort(ERA-JUMP) Study(n=730;American:n=417;Japanese:n=313;from population-representative samples in Pittsburgh,Pennsylvania,USA,and Kusatsu,Shiga,Japan) were compared.The relationships between PA levels and MetS(overall and specific components) in/across the American and Japanese sub-cohorts(adjusting for age,smoking,and alcohol consumption)were also assessed using the same instruments(pedometer and validated questionnaire) to measure PA in both cohorts.Results:A total of 510 individuals provided complete data on PA(American:n=265;Japanese:n=245).The American cohort had significantly lower mean±SD steps/day(7878±3399 steps/day) vs.the Japanese cohort(9055±3797 steps/day)(p <0.001) but had significantly higher self-reported moderate-vigorous leisure PA(American:15.9(7.4-30.3) metabolic task equivalent hours per week(MET-b/week) vs.Japanese:4.0(0-11.3) METh/week,p <0.0001).In both sub-cohorts,each 1000 steps/day increase was associated with lower odds of having MetS(American:OR=0.90,95%CI:0.83-0.98;Japanese:OR=0.87,95%CI:0.79-0.95) and the individual MetS component of high waist circumference(American:OR=0.86,95%CI:0.79-0.94;Japanese:OR=0.87,95%CI:0.80-0.95).In the American cohort only,higher self-reported leisure PA(Met-b/week) was associated with lower odds of MetS and high waist circumference(OR=0.98,95%CI:0.97-0.99 for MetS and waist circumference,respectively).Conclusion:Higher total step counts/day had an importa展开更多
文摘Organomercury lyase (MerB) overexpressed in <em>Escherichia coli</em> captured and decomposed organomercury compounds, and it has been detected by radioactive analysis with neutron irradiation. Genetically modified <em>E. coli</em> captures a lot of mercury from a cultivation solution with about 80% recovery, when the bacteria are growing during 24 to 72 hours. Since the modified <em>E. coli</em> has no additive gene for mercury metabolism, the bacteria could hold mercury tightly by the MerB enzyme in their cell and do not release them into medium. In the later, 72 hours after, bacteria have less recovery ratio;it may be affected by undecompsed mercury compounds in bacteria growth. The recovery ability of the bacteria would not be changed by addition of the MerB producing reagent (IPTG). A quantitative value of mercury atom is estimated by an emission of <em>γ</em>-ray by reactor neutron from a dried cell or solution on a filter paper, which is available for nondestructive testing of bacteria holding mercury atoms. In this method an efficient recovery system of toxic mercury from a polluted solution has been archived without destruction of samples, so called <em>in-cell</em> analysis.
基金The project partially supported by the Grant-in-Aid for Science Research of MEXT in Japan and by the Collaboration Program of the Laboratory for Complex Energy ProcessesIAEKyoto University
文摘Spontaneous transition of the plasma confinement mode was observed in thehelical-axis heliotron device 'Heliotron J' for three different plasma heating schemes, i.e.ECH-only, NBI-only and the combination of ECH and NBI. The transition seems to occur above a certaincritical density. In addition to the confinement transition, a spontaneous shift of the hittingposition of the divertor plasma flux on the wall was observed. This shift could be related with thechange of the edge field topology caused by non-inductive toroidal currents.
文摘We report herein a rare case of lateral parapatellar synovial plica that developed simultaneously in both knees. A 15-year-old competitive soccer player visited our institution with a six-month history of anterolateral pain and catching sensation in both knee joints. On physical examination, he complained of tenderness along the joint line of bilateral patellofemoral joint (PFJ). Visible and palpable popping was observed at the lateral margin of each patella during active knee motion. Magnetic resonance imaging showed obvious synovial soft nodules in the lateral side of the PFJ. On arthroscopy, a yellowish, thick, tongue-shaped tissue extending transversely from the lateral parapatellar synovium was identified in both knees, and parts of this tissue showed avascular hypertrophy. Arthroscopic findings of both knees are almost symmetrical in anatomical location, but no similar in size. After arthroscopic excision, the patient became asymptomatic. At 24-month follow up, he demonstrated full knee function, without evidence of local recurrence.
文摘Organic soft linear actuators were fabricated using galvanostatic electropolymerization of the polypyrrole (PPy) thin film using a methyl benzoate electrolyte solution of N,N-Diethyl-N-methyl-N-(2-methoxyethyl) ammonium bis (trifluoromethanesulfonyl) imide. The electrochemical deformation behaviors of the PPy actuators were investigated in aqueous solutions of an electrolyte, lithium bis (trifluoromethanesulphonyl) imide (LiTFSI) or sodium chloride (NaCl), containing different concentrations of methanol. The actuating strain of approximately 9% was achieved when the actuator was driven by a potential between –1 and 1 V with the potential sweep rate of 10 mV/s corresponding to 0.0025 Hz in the LiTFSI electrolyte containing 40% to 50% of methanol under a load stress of 0.3 MPa. However, the PPy actuator could not catch up with the higher frequency. On the other hand, the PPy actuator caught up with the potential sweep up to 0.1 Hz in the NaCl solutions with a methanol concentration between 40% and 60% with the expense of the actuating strain to approximately 1%.
基金funded by R01 HL68200 from the U.S.National Institutes of Healthby B 16790335 and A 13307016,17209023,and 21249043 from the Japanese Ministry of Education,Culture,Sports,Science,and Technology。
文摘Background:Metabolic syndrome(MetS) is a global health problem.Physical activity(PA) is a known modifiable risk factor for MetS and individual MetS components.However,the role of PA could differ between sub-populations due to differences in the variability of PA and other MetS risk factors.To examine these differences,multi-country studies with standardized outcome measurement methods across cohorts are needed.Methods:Cross-sectional PA levels(total and domain specific) in healthy middle-aged(44-56 years) men in the Risk Factor Assessment among Japanese and U.S.Men in the Post-World War Ⅱ Birth Cohort(ERA-JUMP) Study(n=730;American:n=417;Japanese:n=313;from population-representative samples in Pittsburgh,Pennsylvania,USA,and Kusatsu,Shiga,Japan) were compared.The relationships between PA levels and MetS(overall and specific components) in/across the American and Japanese sub-cohorts(adjusting for age,smoking,and alcohol consumption)were also assessed using the same instruments(pedometer and validated questionnaire) to measure PA in both cohorts.Results:A total of 510 individuals provided complete data on PA(American:n=265;Japanese:n=245).The American cohort had significantly lower mean±SD steps/day(7878±3399 steps/day) vs.the Japanese cohort(9055±3797 steps/day)(p <0.001) but had significantly higher self-reported moderate-vigorous leisure PA(American:15.9(7.4-30.3) metabolic task equivalent hours per week(MET-b/week) vs.Japanese:4.0(0-11.3) METh/week,p <0.0001).In both sub-cohorts,each 1000 steps/day increase was associated with lower odds of having MetS(American:OR=0.90,95%CI:0.83-0.98;Japanese:OR=0.87,95%CI:0.79-0.95) and the individual MetS component of high waist circumference(American:OR=0.86,95%CI:0.79-0.94;Japanese:OR=0.87,95%CI:0.80-0.95).In the American cohort only,higher self-reported leisure PA(Met-b/week) was associated with lower odds of MetS and high waist circumference(OR=0.98,95%CI:0.97-0.99 for MetS and waist circumference,respectively).Conclusion:Higher total step counts/day had an importa
