Endoplasmic reticulum(ER)stress,as an emerging hallmark feature of cancer,has a considerable impact on cell proliferation,metastasis,invasion,and chemotherapy resistance.Ovarian cancer(OvCa)is one of the leading cause...Endoplasmic reticulum(ER)stress,as an emerging hallmark feature of cancer,has a considerable impact on cell proliferation,metastasis,invasion,and chemotherapy resistance.Ovarian cancer(OvCa)is one of the leading causes of cancer-related mortality across the world due to the late stage of disease at diagnosis.Studies have explored the influence of ER stress on OvCa in recent years,while the predictive role of ER stress-related genes in OvCa prognosis remains unexplored.Here,we enrolled 552 cases of ER stress-related genes involved in OvCa from The Cancer Genome Atlas(TCGA)and Gene Expression Omnibus(GEO)cohorts for the screening of prognosis-related genes.The least absolute shrinkage and selection operator(LASSO)regression was applied to establish an ER stress-related risk signature based on the TCGA cohort.A seven-gene signature revealed a favorable predictive efficacy for the TCGA,International Cancer Genome Consortium(ICGC),and another GEO cohort(P<0.001,P<0.001,and P=0.04,respectively).Moreover,functional annotation indicated that this signature was enriched in cellular response and senescence,cytokines interaction,as well as multiple immune-associated terms.The immune infiltration profiles further delineated an immunologic unresponsive status in the high-risk group.In conclusion,ER stress-related genes are vital factors predicting the prognosis of OvCa,and possess great application potential in the clinic.展开更多
以土壤线虫群落组成及结构为研究对象,研究我国黄淮海平原冬小麦样地土壤线虫群落对施肥量变化的动态响应模式.实验采用随机区组设计,设置4种施肥处理,分别为N1:50 kg N·ha-1·yr-1, N2:100 kg N·ha-1·yr-1, N3:150 ...以土壤线虫群落组成及结构为研究对象,研究我国黄淮海平原冬小麦样地土壤线虫群落对施肥量变化的动态响应模式.实验采用随机区组设计,设置4种施肥处理,分别为N1:50 kg N·ha-1·yr-1, N2:100 kg N·ha-1·yr-1, N3:150 kg N·ha-1·yr-1及N4:300 kg N·ha-1·yr-1.实验结果表明,随着施肥量的增加,冬小麦的生物量显著增加,但土壤pH值显著下降.土壤线虫的种群密度在冬小麦拔节期达到峰值.随着施氮量的增加,土壤线虫的数量显著增加,但群落的物种丰富度显著下降.施肥量的变化对食细菌性线虫没有影响,但显著降低了杂-捕类线虫的相对数量,N2处理显著提高了植食性线虫的相对数量,而N4处理显著降低了食真菌性线虫的相对数量.以上实验结果表明,土壤线虫群落中不同营养类群对施肥量变化的响应并不相同.随着施肥量的增加,土壤线虫群落趋向简单化.以上实验结果对于寻求我国黄淮海平原冬小麦产区最佳施肥方案及田间虫害防控具有一定指导意义.展开更多
通过异种材料Q235钢板和5083铝板进行自冲铆接,分别研究了组合方式、板厚、接头热处理(模拟车身烘烤过程)等工艺因素对接头力学性能的影响,结果表明:5083铝板作为下板时接头的性能更优,并且Q235上板板厚对接头的性能有一定的优化作用....通过异种材料Q235钢板和5083铝板进行自冲铆接,分别研究了组合方式、板厚、接头热处理(模拟车身烘烤过程)等工艺因素对接头力学性能的影响,结果表明:5083铝板作为下板时接头的性能更优,并且Q235上板板厚对接头的性能有一定的优化作用.在该实验中,接头b#的组合方式是较优的工艺参数,即1.5 mm Q235-2.0 mm5083;经过烘烤后接头的失效载荷和失效位移都有不同程度的增加,其中性能较优的接头b#经烘烤后失效载荷提升了5.80%,失效位移提升了8.26%;汽车车身涂装过程中进行烘烤作业对接头的性能不会造成强度损失,相反还会对接头力学性能和稳定性有一定程度的优化作用.展开更多
基金This work was supported by the Shanghai Shenkang Hospital Development Center’s Shenkang Promotion of Clin‑ical Skills and Clinical Innovation in Municipal Hospitals Three-Year Action Plan(No.2020‒2023)the Major Clinical Research Project(No.SHDC2020CR1048B)the Pilot Construction Project of High-Level Universities in Shanghai(No.DGF501017-06),China。
文摘Endoplasmic reticulum(ER)stress,as an emerging hallmark feature of cancer,has a considerable impact on cell proliferation,metastasis,invasion,and chemotherapy resistance.Ovarian cancer(OvCa)is one of the leading causes of cancer-related mortality across the world due to the late stage of disease at diagnosis.Studies have explored the influence of ER stress on OvCa in recent years,while the predictive role of ER stress-related genes in OvCa prognosis remains unexplored.Here,we enrolled 552 cases of ER stress-related genes involved in OvCa from The Cancer Genome Atlas(TCGA)and Gene Expression Omnibus(GEO)cohorts for the screening of prognosis-related genes.The least absolute shrinkage and selection operator(LASSO)regression was applied to establish an ER stress-related risk signature based on the TCGA cohort.A seven-gene signature revealed a favorable predictive efficacy for the TCGA,International Cancer Genome Consortium(ICGC),and another GEO cohort(P<0.001,P<0.001,and P=0.04,respectively).Moreover,functional annotation indicated that this signature was enriched in cellular response and senescence,cytokines interaction,as well as multiple immune-associated terms.The immune infiltration profiles further delineated an immunologic unresponsive status in the high-risk group.In conclusion,ER stress-related genes are vital factors predicting the prognosis of OvCa,and possess great application potential in the clinic.
文摘以土壤线虫群落组成及结构为研究对象,研究我国黄淮海平原冬小麦样地土壤线虫群落对施肥量变化的动态响应模式.实验采用随机区组设计,设置4种施肥处理,分别为N1:50 kg N·ha-1·yr-1, N2:100 kg N·ha-1·yr-1, N3:150 kg N·ha-1·yr-1及N4:300 kg N·ha-1·yr-1.实验结果表明,随着施肥量的增加,冬小麦的生物量显著增加,但土壤pH值显著下降.土壤线虫的种群密度在冬小麦拔节期达到峰值.随着施氮量的增加,土壤线虫的数量显著增加,但群落的物种丰富度显著下降.施肥量的变化对食细菌性线虫没有影响,但显著降低了杂-捕类线虫的相对数量,N2处理显著提高了植食性线虫的相对数量,而N4处理显著降低了食真菌性线虫的相对数量.以上实验结果表明,土壤线虫群落中不同营养类群对施肥量变化的响应并不相同.随着施肥量的增加,土壤线虫群落趋向简单化.以上实验结果对于寻求我国黄淮海平原冬小麦产区最佳施肥方案及田间虫害防控具有一定指导意义.
文摘通过异种材料Q235钢板和5083铝板进行自冲铆接,分别研究了组合方式、板厚、接头热处理(模拟车身烘烤过程)等工艺因素对接头力学性能的影响,结果表明:5083铝板作为下板时接头的性能更优,并且Q235上板板厚对接头的性能有一定的优化作用.在该实验中,接头b#的组合方式是较优的工艺参数,即1.5 mm Q235-2.0 mm5083;经过烘烤后接头的失效载荷和失效位移都有不同程度的增加,其中性能较优的接头b#经烘烤后失效载荷提升了5.80%,失效位移提升了8.26%;汽车车身涂装过程中进行烘烤作业对接头的性能不会造成强度损失,相反还会对接头力学性能和稳定性有一定程度的优化作用.
