神经退行性疾病是人类最难治疗的疾病之一.易获取的间充质干细胞(MSCs)可用于自体干细胞移植,间充质干细胞治疗被认为是最有希望的治疗选择之一.然而,传统的生长诱导因子难以实现间充质干细胞的神经分化.电刺激方式可以诱导间充质干细...神经退行性疾病是人类最难治疗的疾病之一.易获取的间充质干细胞(MSCs)可用于自体干细胞移植,间充质干细胞治疗被认为是最有希望的治疗选择之一.然而,传统的生长诱导因子难以实现间充质干细胞的神经分化.电刺激方式可以诱导间充质干细胞的神经分化,但外部电线和复杂的设备给临床治疗带来了很大阻碍.在这项研究中,基于磁电感应效应,我们发现在无需任何生物或化学因子的辅助下,旋转磁场(RMF)驱动下还原氧化石墨烯膜(rGO-M)上产生的无线电信号可以诱导间充质干细胞的神经元样分化.体外实验结果显示,RMF以400 r min^(-1)的速度刺激rGO-M,每天刺激15 min,rGO-M上的MSCs能表达神经元特异性基因和蛋白,连续处理15天后,基因和蛋白的表达量得到明显提升.大鼠体内实验证实,rGO-M上的外源性间充质干细胞可以在旋转磁场的驱动下分化成神经元样细胞.鉴于rGO-M和自体间充质干细胞来源成本较低,rGO-M介导的无线电刺激方法为神经退行性疾病的干细胞治疗提供了一个可行的方案.展开更多
Small cell lung cancer(SCLC) represents a group of highly malignant tumors that give rise to early and widespread metastases at the time of diagnosis.The preferential metastatic sites are the brain,liver,adrenal gland...Small cell lung cancer(SCLC) represents a group of highly malignant tumors that give rise to early and widespread metastases at the time of diagnosis.The preferential metastatic sites are the brain,liver,adrenal glands,bone,and bone marrow.However,metastases of the gastrointestinal system,especially the stomach,are rare; most cases of stomach metastasis are asymptomatic and,as a result,are usually only discovered at autopsy.We report a case of gastric metastasis originating from SCLC.The patient was a 66-year-old man admitted to our hospital due to abdominal pain.He underwent gastroscopy,with the pathological report of the tissue biopsy proving it to be a small cell cancer.Immunohistochemistry was positive for CD56,synaptophysin,and pan-cytokeratin.These results confirmed the diagnosis of gastric metastasis of a neuroendocrine small cell carcinoma from the lung.展开更多
Elastic wave propagation and attenuation in porous rock layers with oriented sets of fractures, especially in carbonate reservoirs, are anisotropic owing to fracture sealing, fracture size, fracture density, filling f...Elastic wave propagation and attenuation in porous rock layers with oriented sets of fractures, especially in carbonate reservoirs, are anisotropic owing to fracture sealing, fracture size, fracture density, filling fluid, and fracture strike orientation. To address this problem, we adopt the Chapman effective medium model and carry out numerical experiments to assess the variation in P-wave velocity and attenuation, and the shear-wave splitting anisotropy with the frequency and azimuth of the incident wave. The results suggest that velocity, attenuation, and anisotropy vary as function of azimuth and frequency. The azimuths of the minimum attenuation and maximum P-wave velocity are nearly coincident with the average strike of the two sets of open fractures. P-wave velocity is greater in sealed fractures than open fractures, whereas the attenuation of energy and anisotropy is stronger in open fractures than sealed fractures. For fractures of different sizes, the maximum velocity together with the minimum attenuation correspond to the average orientation of the fracture sets. Small fractures affect the wave propagation less. Azimuth-dependent anisotropy is low and varies more than the other attributes. Fracture density strongly affects the P-wave velocity, attenuation, and shear-wave anisotropy. The attenuation is more sensitive to the variation of fracture size than that of velocity and anisotropy. In the seismic frequency band, the effect of oil and gas saturation on attenuation is very different from that for brine saturation and varies weakly over azimuth. It is demonstrated that for two sets of fractures with the same density, the fast shear-wave polarization angle is almost linearly related with the orientation of one of the fracture sets.展开更多
基金supported by the National Natural Science Foundation of China(51972148,52272212,and 11904131)Shandong Provincial Natural Science Foundation(ZR2020KE056 and ZR2021YQ04)+1 种基金the Major Scientific and Technological Innovation Project of Shandong Province(2021CXGC010603)the Collaborative Innovation Center of Technology and Equipment for Biological Diagnosis and Therapy in Universities of Shandong。
文摘神经退行性疾病是人类最难治疗的疾病之一.易获取的间充质干细胞(MSCs)可用于自体干细胞移植,间充质干细胞治疗被认为是最有希望的治疗选择之一.然而,传统的生长诱导因子难以实现间充质干细胞的神经分化.电刺激方式可以诱导间充质干细胞的神经分化,但外部电线和复杂的设备给临床治疗带来了很大阻碍.在这项研究中,基于磁电感应效应,我们发现在无需任何生物或化学因子的辅助下,旋转磁场(RMF)驱动下还原氧化石墨烯膜(rGO-M)上产生的无线电信号可以诱导间充质干细胞的神经元样分化.体外实验结果显示,RMF以400 r min^(-1)的速度刺激rGO-M,每天刺激15 min,rGO-M上的MSCs能表达神经元特异性基因和蛋白,连续处理15天后,基因和蛋白的表达量得到明显提升.大鼠体内实验证实,rGO-M上的外源性间充质干细胞可以在旋转磁场的驱动下分化成神经元样细胞.鉴于rGO-M和自体间充质干细胞来源成本较低,rGO-M介导的无线电刺激方法为神经退行性疾病的干细胞治疗提供了一个可行的方案.
基金Supported by Shandong Cancer Hospital and Institute,Jinan,Shandong Province,China
文摘Small cell lung cancer(SCLC) represents a group of highly malignant tumors that give rise to early and widespread metastases at the time of diagnosis.The preferential metastatic sites are the brain,liver,adrenal glands,bone,and bone marrow.However,metastases of the gastrointestinal system,especially the stomach,are rare; most cases of stomach metastasis are asymptomatic and,as a result,are usually only discovered at autopsy.We report a case of gastric metastasis originating from SCLC.The patient was a 66-year-old man admitted to our hospital due to abdominal pain.He underwent gastroscopy,with the pathological report of the tissue biopsy proving it to be a small cell cancer.Immunohistochemistry was positive for CD56,synaptophysin,and pan-cytokeratin.These results confirmed the diagnosis of gastric metastasis of a neuroendocrine small cell carcinoma from the lung.
基金This study was supported by the National Natural Science Foundation of China Rsearch (Nos. 41674046, 41440030, and 41574078) and the Fundamental Research Funds for the Central Universities of Lanzhou university (No. lzujbky-2015-175).
文摘Elastic wave propagation and attenuation in porous rock layers with oriented sets of fractures, especially in carbonate reservoirs, are anisotropic owing to fracture sealing, fracture size, fracture density, filling fluid, and fracture strike orientation. To address this problem, we adopt the Chapman effective medium model and carry out numerical experiments to assess the variation in P-wave velocity and attenuation, and the shear-wave splitting anisotropy with the frequency and azimuth of the incident wave. The results suggest that velocity, attenuation, and anisotropy vary as function of azimuth and frequency. The azimuths of the minimum attenuation and maximum P-wave velocity are nearly coincident with the average strike of the two sets of open fractures. P-wave velocity is greater in sealed fractures than open fractures, whereas the attenuation of energy and anisotropy is stronger in open fractures than sealed fractures. For fractures of different sizes, the maximum velocity together with the minimum attenuation correspond to the average orientation of the fracture sets. Small fractures affect the wave propagation less. Azimuth-dependent anisotropy is low and varies more than the other attributes. Fracture density strongly affects the P-wave velocity, attenuation, and shear-wave anisotropy. The attenuation is more sensitive to the variation of fracture size than that of velocity and anisotropy. In the seismic frequency band, the effect of oil and gas saturation on attenuation is very different from that for brine saturation and varies weakly over azimuth. It is demonstrated that for two sets of fractures with the same density, the fast shear-wave polarization angle is almost linearly related with the orientation of one of the fracture sets.