The objective of this study was to analyze the response of runoff in the area of runoff yield of the upstream Shiyang River basin to climate change and to promote sustainable development of regional water resources an...The objective of this study was to analyze the response of runoff in the area of runoff yield of the upstream Shiyang River basin to climate change and to promote sustainable development of regional water resources and ecological environment.As the biggest tributary of the Shiyang River,Xiying River is the only hydrological station(Jiutiaoling) that has provincial natural river and can achieve long time series monitoring data in the basin.The data obtained from this station is representative of natural conditions because it has little human activites.This study built a regression model through identifying the characteristics of runoff and climate change by using Mann-Kendall nonparametric statistical test,cumulative anomaly,and correlation analysis.The results show that the average annual runoff is 320.6 million m3/a with the coefficient of variation of 0.18 and shows slightly decrease during 1956-2020.It has a significant positive correlation the average annual precipitation(P<0.01).Runoff is sensitive to climate change,and the climate has becoming warm and wet and annual runoff has entering wet period from 2003.Compared to the earlier period(1955-2000),the increases of average annual temperature,precipitation and runoff in recent two decades were 15%,9.3%,and 7.8%,respectively.Runoff in the Shiyang River is affected by temperature and precipitation among climate factors,and the simulation results of the runoff-climate response model(R=0.0052P-0.1589T+2.373) indicate that higher temperature leads to a weakening of the ecological regulation of surface runoff in the flow-producing area.展开更多
BACKGROUND Gut microbiota dysbiosis is reportedly actively involved in autoimmune diseases such as type 1 diabetes mellitus(T1DM).However,the alterations in the gut microbiota and their correlation with fasting blood ...BACKGROUND Gut microbiota dysbiosis is reportedly actively involved in autoimmune diseases such as type 1 diabetes mellitus(T1DM).However,the alterations in the gut microbiota and their correlation with fasting blood glucose(FBG)in Chinese children with T1DM remain unclear.AIM To investigate alterations in the gut microbiota in Chinese children with T1DM and their associations with clinical indicators.METHODS Samples from 51 children with T1DM and 47 age-matched and gender-matched healthy controls were obtained,to explore the structural and functional alterations in the fecal microbiota.The V3-V4 regions of the 16S rRNA gene were sequenced on a MiSeq instrument,and the association with FBG were analyzed.RESULTS We found that the bacterial diversity was significantly increased in the T1DMassociated fecal microbiota,and changes in the microbial composition were observed at different taxonomic levels.The T1DM-reduced differential taxa,such as Bacteroides vulgatus ATCC8482,Bacteroides ovatus,Bacteroides xylanisolvens,and Flavonifractor plautii,were negatively correlated with FBG,while the T1DMenriched taxa,such as Blautia,Eubacterium hallii group,Anaerostipes hadrus,and Dorea longicatena,were positively correlated with FBG.Bacteroides vulgatus ATCC8482,Bacteroides ovatus,the Eubacterium hallii group,and Anaerostipes hadrus,either alone or in combination,could be used as noninvasive diagnostic biomarkers to discriminate children with T1DM from healthy controls.In addition,the functional changes in the T1DM-associated fecal microbiota also suggest that these fecal microbes were associated with altered functions and metabolic activities,such as glycan biosynthesis and metabolism and lipid metabolism,which might play vital roles in the pathogenesis and development of T1DM.CONCLUSION Our present comprehensive investigation of the T1DM-associated fecal microbiota provides novel insights into the pathogenesis of the disease and sheds light on the diagnosis and treatment of T1DM.展开更多
The advent of industrial civilization has brought about enormous materials advancements;yet it has also caused a rapid depletion of natural resources,leading to global energy crisis and environmental pollution.Facing ...The advent of industrial civilization has brought about enormous materials advancements;yet it has also caused a rapid depletion of natural resources,leading to global energy crisis and environmental pollution.Facing human civilization,one fundamental issue stands at its core:how can we achieve a harmonious coexistence between humanity and nature?This question has become the key challenge of our time,demanding our utmost attention and concerted efforts.展开更多
Liu X,Cheng YW,Shao L,Sun SH,Wu J,Song QH,Zou HS,Ling ZX.Gut microbiota dysbiosis in Chinese children with type 1 diabetes mellitus:An observational study.World J Gastroenterol 2021;27(19):2394-2414[PMID:34040330 DOI:...Liu X,Cheng YW,Shao L,Sun SH,Wu J,Song QH,Zou HS,Ling ZX.Gut microbiota dysbiosis in Chinese children with type 1 diabetes mellitus:An observational study.World J Gastroenterol 2021;27(19):2394-2414[PMID:34040330 DOI:10.3748/wjg.v27.i19.2394].In this article,the affiliation of the first author was wrong.Xia Liu,the first author,belonged to Department of Intensive Care Unit,The First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou,310003,China.展开更多
Background Epithelial-mesenchymal transition is a cellular process characterized by the loss of cell adhesion, inhibition of E-cadherin expression, and increased cell mobility. Cells without Napsin A are susceptible t...Background Epithelial-mesenchymal transition is a cellular process characterized by the loss of cell adhesion, inhibition of E-cadherin expression, and increased cell mobility. Cells without Napsin A are susceptible to transition. Further studies are required to investigate whether this transition can be reversed by restoration of Napsin A. Methods A Napsin A expression vector PLJM1-Napsin A plasmid was constructed and then transfected into the epithelial cell line A549 by lentivirus transfection to obtain A549-PLJM1-Napsin A cell line. Cell proliferation was assayed by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide and cell cycle was measured by flow cytometry. The E-cadherin, type I collagen, and focal adhesion kinase mRNA level was detected by reverse transcription-polymerase chain reaction. The Napsin A, E-cadherin, type I collagen, and focal adhesion kinase protein level in A549 cells was detected by Western blotting. Results Transforming growth factor-β1 induced epithelial-mesenchymal transition in A549 cells, as demonstrated by significant reduction of E-cadherin mRNA and protein levels （P 〈0.01） as well as up-regulation of type I collagen （P 〈0.01）. Transfection of Napsin A in A549 cells can partially block the transforming growth factor-β1-regulated expression of E-cadherin and type I collagen （P 〈0.01）. In addition, transforming growth factor-β1-induced cell proliferation was inhibited by Napsin A （P 〈0.01）. Further study demonstrated that Napsin A caused G0/G1 arrest and inhibited the expression of focal adhesion kinase （P 〈0.01）, a key protein in the integrin signaling pathway, in the in vitro epithelial-mesenchymal transition model. Conclusions Sustained Napsin A expression in A549 cells can inhibit the transforming growth factor-β1-induced epithelial-mesenchymal transition. This may be due to the Napsin A-mediated inhibition of focal adhesion kinase expression and integrin signaling pathway.展开更多
基金the Geological Survey Project of China(Hydrogeology and Water Resources Survey and Monitoring in Hexi Corridor,China.No.DD20221752-2).
文摘The objective of this study was to analyze the response of runoff in the area of runoff yield of the upstream Shiyang River basin to climate change and to promote sustainable development of regional water resources and ecological environment.As the biggest tributary of the Shiyang River,Xiying River is the only hydrological station(Jiutiaoling) that has provincial natural river and can achieve long time series monitoring data in the basin.The data obtained from this station is representative of natural conditions because it has little human activites.This study built a regression model through identifying the characteristics of runoff and climate change by using Mann-Kendall nonparametric statistical test,cumulative anomaly,and correlation analysis.The results show that the average annual runoff is 320.6 million m3/a with the coefficient of variation of 0.18 and shows slightly decrease during 1956-2020.It has a significant positive correlation the average annual precipitation(P<0.01).Runoff is sensitive to climate change,and the climate has becoming warm and wet and annual runoff has entering wet period from 2003.Compared to the earlier period(1955-2000),the increases of average annual temperature,precipitation and runoff in recent two decades were 15%,9.3%,and 7.8%,respectively.Runoff in the Shiyang River is affected by temperature and precipitation among climate factors,and the simulation results of the runoff-climate response model(R=0.0052P-0.1589T+2.373) indicate that higher temperature leads to a weakening of the ecological regulation of surface runoff in the flow-producing area.
基金National Natural Science Foundation of China,No.31700800,No.81771724,and No.81790631National S&T Major Project of China,No.2018YFC2000500.
文摘BACKGROUND Gut microbiota dysbiosis is reportedly actively involved in autoimmune diseases such as type 1 diabetes mellitus(T1DM).However,the alterations in the gut microbiota and their correlation with fasting blood glucose(FBG)in Chinese children with T1DM remain unclear.AIM To investigate alterations in the gut microbiota in Chinese children with T1DM and their associations with clinical indicators.METHODS Samples from 51 children with T1DM and 47 age-matched and gender-matched healthy controls were obtained,to explore the structural and functional alterations in the fecal microbiota.The V3-V4 regions of the 16S rRNA gene were sequenced on a MiSeq instrument,and the association with FBG were analyzed.RESULTS We found that the bacterial diversity was significantly increased in the T1DMassociated fecal microbiota,and changes in the microbial composition were observed at different taxonomic levels.The T1DM-reduced differential taxa,such as Bacteroides vulgatus ATCC8482,Bacteroides ovatus,Bacteroides xylanisolvens,and Flavonifractor plautii,were negatively correlated with FBG,while the T1DMenriched taxa,such as Blautia,Eubacterium hallii group,Anaerostipes hadrus,and Dorea longicatena,were positively correlated with FBG.Bacteroides vulgatus ATCC8482,Bacteroides ovatus,the Eubacterium hallii group,and Anaerostipes hadrus,either alone or in combination,could be used as noninvasive diagnostic biomarkers to discriminate children with T1DM from healthy controls.In addition,the functional changes in the T1DM-associated fecal microbiota also suggest that these fecal microbes were associated with altered functions and metabolic activities,such as glycan biosynthesis and metabolism and lipid metabolism,which might play vital roles in the pathogenesis and development of T1DM.CONCLUSION Our present comprehensive investigation of the T1DM-associated fecal microbiota provides novel insights into the pathogenesis of the disease and sheds light on the diagnosis and treatment of T1DM.
文摘The advent of industrial civilization has brought about enormous materials advancements;yet it has also caused a rapid depletion of natural resources,leading to global energy crisis and environmental pollution.Facing human civilization,one fundamental issue stands at its core:how can we achieve a harmonious coexistence between humanity and nature?This question has become the key challenge of our time,demanding our utmost attention and concerted efforts.
文摘Liu X,Cheng YW,Shao L,Sun SH,Wu J,Song QH,Zou HS,Ling ZX.Gut microbiota dysbiosis in Chinese children with type 1 diabetes mellitus:An observational study.World J Gastroenterol 2021;27(19):2394-2414[PMID:34040330 DOI:10.3748/wjg.v27.i19.2394].In this article,the affiliation of the first author was wrong.Xia Liu,the first author,belonged to Department of Intensive Care Unit,The First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou,310003,China.
文摘Background Epithelial-mesenchymal transition is a cellular process characterized by the loss of cell adhesion, inhibition of E-cadherin expression, and increased cell mobility. Cells without Napsin A are susceptible to transition. Further studies are required to investigate whether this transition can be reversed by restoration of Napsin A. Methods A Napsin A expression vector PLJM1-Napsin A plasmid was constructed and then transfected into the epithelial cell line A549 by lentivirus transfection to obtain A549-PLJM1-Napsin A cell line. Cell proliferation was assayed by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide and cell cycle was measured by flow cytometry. The E-cadherin, type I collagen, and focal adhesion kinase mRNA level was detected by reverse transcription-polymerase chain reaction. The Napsin A, E-cadherin, type I collagen, and focal adhesion kinase protein level in A549 cells was detected by Western blotting. Results Transforming growth factor-β1 induced epithelial-mesenchymal transition in A549 cells, as demonstrated by significant reduction of E-cadherin mRNA and protein levels （P 〈0.01） as well as up-regulation of type I collagen （P 〈0.01）. Transfection of Napsin A in A549 cells can partially block the transforming growth factor-β1-regulated expression of E-cadherin and type I collagen （P 〈0.01）. In addition, transforming growth factor-β1-induced cell proliferation was inhibited by Napsin A （P 〈0.01）. Further study demonstrated that Napsin A caused G0/G1 arrest and inhibited the expression of focal adhesion kinase （P 〈0.01）, a key protein in the integrin signaling pathway, in the in vitro epithelial-mesenchymal transition model. Conclusions Sustained Napsin A expression in A549 cells can inhibit the transforming growth factor-β1-induced epithelial-mesenchymal transition. This may be due to the Napsin A-mediated inhibition of focal adhesion kinase expression and integrin signaling pathway.
