Growth plate cartilage has limited self-repair ability,leading to poor bone bridge formation post-injury and ultimately limb growth defects in children.The current corrective surgeries are highly invasive,and outcomes...Growth plate cartilage has limited self-repair ability,leading to poor bone bridge formation post-injury and ultimately limb growth defects in children.The current corrective surgeries are highly invasive,and outcomes can be unpredictable.Following growth plate injury,the direct loss of extracellular matrix(ECM)coupled with further ECM depletion due to the inhibitory effects of inflammation on the cartilage matrix protein greatly hinder chondrocyte regeneration.We designed an exosome(Exo)derived from bone marrow mesenchymal stem cells(BMSCs)loaded ECM-mimic hydrogel to promote cartilage repair by directly supplementing ECM and anti-inflammatory properties.Aldehyde-functionalized chondroitin sulfate(OCS)was introduced into gelatin methacryloyl(GM)to form GMOCS hydrogel.Our results uncovered that GMOCS hydrogel could significantly promote the synthesis of ECM due to the doping of OCS.In addition,the GMOCS-Exos hydrogel could further promote the anabolism of chondrocytes by inhibiting inflammation and ultimately promote growth plate injury repair through ECM remodeling.展开更多
Artificial vascular graft(AVG)fistula is widely used for hemodialysis treatment in patients with renal failure.However,it has poor elasticity and compliance,leading to stenosis and thrombosis.The ideal artificial bloo...Artificial vascular graft(AVG)fistula is widely used for hemodialysis treatment in patients with renal failure.However,it has poor elasticity and compliance,leading to stenosis and thrombosis.The ideal artificial blood vessel for dialysis should replicate the structure and components of a real artery,which is primarily maintained by collagen in the extracellular matrix(ECM)of arterial cells.Studies have revealed that in hepatitis B virus(HBV)-induced liver fibrosis,hepatic stellate cells(HSCs)become hyperactive and produce excessive ECM fibers.Furthermore,mechanical stimulation can encourage ECM secretion and remodeling of a fiber structure.Based on the above factors,we transfected HSCs with the hepatitis B viral X(HBX)gene for simulating the process of HBV infection.Subsequently,these HBX-HSCs were implanted into a polycaprolactonepolyurethane(PCL-PU)bilayer scaffold in which the inner layer is dense and the outer layer consists of pores,which was mechanically stimulated to promote the secretion of collagen nanofiber from the HBX-HSCs and to facilitate crosslinking with the scaffold.We obtained an ECM-PCL-PU composite bionic blood vessel that could act as access for dialysis after decellularization.Then,the vessel scaffold was implanted into a rabbit’s neck arteriovenous fistula model.It exhibited strong tensile strength and smooth blood flow and formed autologous blood vessels in the rabbit’s body.Our study demonstrates the use of human cells to create biomimetic dialysis blood vessels,providing a novel approach for creating clinical vascular access for dialysis.展开更多
Curcumin, the medically active component from Curcuma Tonga (Turmeric), is widely used to treat inflammatory diseases. Protein interaction network (PIN) analysis was used to predict its mechanisms of molecular action....Curcumin, the medically active component from Curcuma Tonga (Turmeric), is widely used to treat inflammatory diseases. Protein interaction network (PIN) analysis was used to predict its mechanisms of molecular action. Targets of curcumin were obtained based on ChEMBL and STITCH databases. Protein protein interactions (PPIs) were extracted from the String database. The PIN of curcumin was constructed by Cytoscape and the function modules identified by gene ontology (GO) enrichment analysis based on molecular complex detection (MCODE). A PIN of curcumin with 482 nodes and 1688 interactions was constructed, which has scale-free, small world and modular properties. Based on analysis of these function modules, the mechanism of curcumin is proposed. Two modules were found to be intimately associated with inflammation. With function modules analysis, the anti-inflammatory effects of curcumin were related to SMAD, ERG and mediation by the TLR family. TLR9 may be a potential target of curcumin to treat inflammation. (C) 2015 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V.展开更多
As one of the most common forms of skin injuries,skin burns are often accompanied by edema pain,suppuration of infection,slow tissue regeneration,and severe scar formation,which significantly delay wound healing as we...As one of the most common forms of skin injuries,skin burns are often accompanied by edema pain,suppuration of infection,slow tissue regeneration,and severe scar formation,which significantly delay wound healing as well as affect the quality of life.We prepared multifunctional electrospun poly(L-lactide-co-glycolide)/gelatin(P/G)-based dressings to synergistically harness the therapeutic benefits of peppermint essential oil(T),burn ointment(B),and Oregano essential oil(O)(P/G@TBO)for skin regeneration in punch and burn injury models.The P/G@TBO can afford the sustained release of bioactive cues for up to 72 h as well as remarkably promote cell migration(ca.P/G@TBO,89%vs.control group,51%)at 24 h.The P/G@TBO membranes also showed significant angiogenic effect as well as antibacterial and anti-inflammatory properties than that of the control group in vitro.Moreover,P/G@TBO dressings enabled fast wound healing(ca.P/G@TBO,100%wound closure vs.control group,95%)in a full-thickness excisional defect model up to 14 days in rats.Further evaluation of membranes in different animal models,including tail wagging model,facial itch model,and hot burn injury model showed significant pain relieve effect as well as itching and swelling relief functions during earlier stages of wound healing.Membranes were next transplanted into a scalded wound model in rats and an ear punch wound model in rabbits,which manifested antibacterial and anti-inflammatory properties and promoted re-epithelialization to achieve scarless wound healing percentage wound closure at day 28:P/G@TBO,96%vs.control group 66%.Taken together our approach of simultaneously harnessing T,B,and O to enable multifunctionality to fibrous dressings may hold great promise for burn wound healing applications and other related disciplines.展开更多
文摘SAR影像对于水体和地表形变具有很好的辨识性,因此常用来进行水体识别、土壤湿度反演和地表形变检测研究与应用。利用载有C波段合成孔径雷达的Sentinel-1卫星数据对大范围的水体信息进行识别,提出了SDWI(Sentinel-1 Dual-Polarized Water Index)水体信息提取方法。该方法受到NDVI和NDWI方法的启发,结合微波遥感中水体信息在影像中的特点,进一步研究了Sentinel-1双极化数据(VV和VH)之间水体信息提取的关系,以此关系达到增强水体特征的目的,同时消除土壤和植被的存在。分别以Sentinel-1A巢湖区域和Sentinel-1B鄱阳湖区域SAR影像为例来提取水体信息,实验结果表明该方法显著有效,但对影像中阴影的处理是未来研究的难点。
基金supported by the Natural Science Foundation of Guangdong Province(No.2020A1515011369).
文摘Growth plate cartilage has limited self-repair ability,leading to poor bone bridge formation post-injury and ultimately limb growth defects in children.The current corrective surgeries are highly invasive,and outcomes can be unpredictable.Following growth plate injury,the direct loss of extracellular matrix(ECM)coupled with further ECM depletion due to the inhibitory effects of inflammation on the cartilage matrix protein greatly hinder chondrocyte regeneration.We designed an exosome(Exo)derived from bone marrow mesenchymal stem cells(BMSCs)loaded ECM-mimic hydrogel to promote cartilage repair by directly supplementing ECM and anti-inflammatory properties.Aldehyde-functionalized chondroitin sulfate(OCS)was introduced into gelatin methacryloyl(GM)to form GMOCS hydrogel.Our results uncovered that GMOCS hydrogel could significantly promote the synthesis of ECM due to the doping of OCS.In addition,the GMOCS-Exos hydrogel could further promote the anabolism of chondrocytes by inhibiting inflammation and ultimately promote growth plate injury repair through ECM remodeling.
基金supported by the National Natural Science Foundation of China(No.81770294)the Natural Science Foundation of Fujian Province(No.2023J05261),China.
文摘Artificial vascular graft(AVG)fistula is widely used for hemodialysis treatment in patients with renal failure.However,it has poor elasticity and compliance,leading to stenosis and thrombosis.The ideal artificial blood vessel for dialysis should replicate the structure and components of a real artery,which is primarily maintained by collagen in the extracellular matrix(ECM)of arterial cells.Studies have revealed that in hepatitis B virus(HBV)-induced liver fibrosis,hepatic stellate cells(HSCs)become hyperactive and produce excessive ECM fibers.Furthermore,mechanical stimulation can encourage ECM secretion and remodeling of a fiber structure.Based on the above factors,we transfected HSCs with the hepatitis B viral X(HBX)gene for simulating the process of HBV infection.Subsequently,these HBX-HSCs were implanted into a polycaprolactonepolyurethane(PCL-PU)bilayer scaffold in which the inner layer is dense and the outer layer consists of pores,which was mechanically stimulated to promote the secretion of collagen nanofiber from the HBX-HSCs and to facilitate crosslinking with the scaffold.We obtained an ECM-PCL-PU composite bionic blood vessel that could act as access for dialysis after decellularization.Then,the vessel scaffold was implanted into a rabbit’s neck arteriovenous fistula model.It exhibited strong tensile strength and smooth blood flow and formed autologous blood vessels in the rabbit’s body.Our study demonstrates the use of human cells to create biomimetic dialysis blood vessels,providing a novel approach for creating clinical vascular access for dialysis.
基金supported by grants from the National Natural Science Foundation of China(Grant No.81403103)Chinese Medicine Resources(Sichuan Province)Youth Science and Technology Innovation Team(Grant No.2015TD0028)+1 种基金Sichuan Province Science and Technology Support Plan(Grant No.2014SZ0156)Sichuan Province Education Department Project(Grant No.2013SZB0781)
文摘Curcumin, the medically active component from Curcuma Tonga (Turmeric), is widely used to treat inflammatory diseases. Protein interaction network (PIN) analysis was used to predict its mechanisms of molecular action. Targets of curcumin were obtained based on ChEMBL and STITCH databases. Protein protein interactions (PPIs) were extracted from the String database. The PIN of curcumin was constructed by Cytoscape and the function modules identified by gene ontology (GO) enrichment analysis based on molecular complex detection (MCODE). A PIN of curcumin with 482 nodes and 1688 interactions was constructed, which has scale-free, small world and modular properties. Based on analysis of these function modules, the mechanism of curcumin is proposed. Two modules were found to be intimately associated with inflammation. With function modules analysis, the anti-inflammatory effects of curcumin were related to SMAD, ERG and mediation by the TLR family. TLR9 may be a potential target of curcumin to treat inflammation. (C) 2015 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V.
基金Science and Technology Commission of Shanghai Municipality(No.20S31900900,20DZ2254900)Sino German Science Foundation Research Exchange Center(M-0263)+8 种基金Taishan Scholars Program of Shandong Province(tsqn201812141)Shandong Provincial Natural Science Foundation(ZR2021MH004)Academic promotion program of Shandong First Medical University(2019RC016)Researchers Supporting Project Number(RSP2023R65)King Saud University,Riyadh,Saudi Arabia,China Education Association for International Exchange(2022181)Donghua University Postgraduate Innovation and Entrepreneurship Ability Training Program(yjssc2023002)Grant-in-Aid for JSPS Research Fellows(JP21F21353)M.S.is an International Research Fellow of the Japan Society for the Promotion of Science(Postdoctoral Fellowships for Research in Japan(Standard))M.S.is keenly grateful to the Gifu University,Japan for Special Cooperative Research Program and College of Biological Science and Medical Engineering,Donghua University,Shanghai,China for cooperative research.
文摘As one of the most common forms of skin injuries,skin burns are often accompanied by edema pain,suppuration of infection,slow tissue regeneration,and severe scar formation,which significantly delay wound healing as well as affect the quality of life.We prepared multifunctional electrospun poly(L-lactide-co-glycolide)/gelatin(P/G)-based dressings to synergistically harness the therapeutic benefits of peppermint essential oil(T),burn ointment(B),and Oregano essential oil(O)(P/G@TBO)for skin regeneration in punch and burn injury models.The P/G@TBO can afford the sustained release of bioactive cues for up to 72 h as well as remarkably promote cell migration(ca.P/G@TBO,89%vs.control group,51%)at 24 h.The P/G@TBO membranes also showed significant angiogenic effect as well as antibacterial and anti-inflammatory properties than that of the control group in vitro.Moreover,P/G@TBO dressings enabled fast wound healing(ca.P/G@TBO,100%wound closure vs.control group,95%)in a full-thickness excisional defect model up to 14 days in rats.Further evaluation of membranes in different animal models,including tail wagging model,facial itch model,and hot burn injury model showed significant pain relieve effect as well as itching and swelling relief functions during earlier stages of wound healing.Membranes were next transplanted into a scalded wound model in rats and an ear punch wound model in rabbits,which manifested antibacterial and anti-inflammatory properties and promoted re-epithelialization to achieve scarless wound healing percentage wound closure at day 28:P/G@TBO,96%vs.control group 66%.Taken together our approach of simultaneously harnessing T,B,and O to enable multifunctionality to fibrous dressings may hold great promise for burn wound healing applications and other related disciplines.
