Cattle production is integral to the people of Africa and her economy. To <span style="font-family:Verdana;">improve cattle productivity, there is a need to inculcate molecular mark</span><spa...Cattle production is integral to the people of Africa and her economy. To <span style="font-family:Verdana;">improve cattle productivity, there is a need to inculcate molecular mark</span><span style="font-family:Verdana;">er-assisted selection into current breeding practices. In this study, we investigated, in </span><span style="font-family:Verdana;">White Fulani and Muturu cattle breeds, polymorphism of two candidate</span><span style="font-family:Verdana;"> genes (Smoothened—</span><i><span style="font-family:Verdana;">SMO</span></i><span style="font-family:Verdana;"> and Lipase Maturation Factor 1—</span><i><span style="font-family:Verdana;">LMF</span></i><span style="font-family:Verdana;">1</span><span style="font-family:Verdana;">) that have been reported to influence body size in cattle. The </span><i><span style="font-family:Verdana;">SMO</span></i><span style="font-family:Verdana;"> gene encodes for the SMO </span><span style="font-family:Verdana;">(smoothened) receptor protein;a membrane signal transducer that plays a</span><span style="font-family:Verdana;"> role in the control of bone formation and adipogenesis. </span><i><span style="font-family:Verdana;">LMF</span></i><span style="font-family:Verdana;">1 </span><span style="font-family:Verdana;">encodes for the </span><span style="font-family:Verdana;">LMF1 protein which regulates post-translational maturation of vascular li</span><span style="font-family:Verdana;">pases. Desired regions of the genes were amplified and genotyped by direct </span><span style="font-family:Verdana;">DNA sequencing or Polymerase chain reaction—Restriction Fragment</span><span style="font-family:Verdana;"> Length </span><span style="font-family:Verdana;">Polymorphism. For association study, linear body measurements traits</span><span style="font-family:Verdana;"> (BMTs) that can be used to predict body size were measured on each animal and values of BMTs measured were compared between both breeds. Very significant (P < 0.001) differences, in favour of White Fulani展开更多
Cattle production is an important aspect of animal agriculture. Disease predisposition in cattle can lead to lowered productivity and poor animal welfare. To select and breed for the fittest cattle population, it is e...Cattle production is an important aspect of animal agriculture. Disease predisposition in cattle can lead to lowered productivity and poor animal welfare. To select and breed for the fittest cattle population, it is expedient that we understand the genetic basis of disease tolerance/resistance. Major histocompatibility complex (MHC) is a vital component of the immune system in vertebrates. Its genes are crucial determinants for immune response and resistance to infectious diseases. The bovine MHC is referred to as Bovine Lymphocyte antigen (BoLA) with its most functional and highly variable region located in the exon 2 (BoLA-DRB3.2). Over 100 alleles of BoLA-DRB3.2 have been identified in cattle and many studies have associated polymorphism in this region with disease resistance/susceptibility. In this study, we investigated the polymorphic nature of BoLA-DRB3.2 in the White Fulani and Muturu cattle breeds using a single PCR-sequence based typing. We identified 26 and 25 alleles in White Fulani and Muturu breeds, respectively, with only six alleles being mutual in the two breeds. Some of the alleles identified in this study have been noted as markers for disease status in cattle. BoLA-DRB3*014:01:01, BoLA-DRB3*011:01, and BoLA-DRB3*008:01 alleles have been associated with Bovine leukemia virus (BLV) resistance in cattle. BoLA-DRB3*014:01:01, BoLA-DRB3*001:01, and BoLA-DRB3*011:01 were linked with mastitis resistance in Japanese Holstein cows. While no inference can be drawn in terms of association with disease status, this study confirms the highly polymorphic and diverse nature of BoLA-DRB3 in White Fulani and Muturu cattle breeds.展开更多
文摘Cattle production is integral to the people of Africa and her economy. To <span style="font-family:Verdana;">improve cattle productivity, there is a need to inculcate molecular mark</span><span style="font-family:Verdana;">er-assisted selection into current breeding practices. In this study, we investigated, in </span><span style="font-family:Verdana;">White Fulani and Muturu cattle breeds, polymorphism of two candidate</span><span style="font-family:Verdana;"> genes (Smoothened—</span><i><span style="font-family:Verdana;">SMO</span></i><span style="font-family:Verdana;"> and Lipase Maturation Factor 1—</span><i><span style="font-family:Verdana;">LMF</span></i><span style="font-family:Verdana;">1</span><span style="font-family:Verdana;">) that have been reported to influence body size in cattle. The </span><i><span style="font-family:Verdana;">SMO</span></i><span style="font-family:Verdana;"> gene encodes for the SMO </span><span style="font-family:Verdana;">(smoothened) receptor protein;a membrane signal transducer that plays a</span><span style="font-family:Verdana;"> role in the control of bone formation and adipogenesis. </span><i><span style="font-family:Verdana;">LMF</span></i><span style="font-family:Verdana;">1 </span><span style="font-family:Verdana;">encodes for the </span><span style="font-family:Verdana;">LMF1 protein which regulates post-translational maturation of vascular li</span><span style="font-family:Verdana;">pases. Desired regions of the genes were amplified and genotyped by direct </span><span style="font-family:Verdana;">DNA sequencing or Polymerase chain reaction—Restriction Fragment</span><span style="font-family:Verdana;"> Length </span><span style="font-family:Verdana;">Polymorphism. For association study, linear body measurements traits</span><span style="font-family:Verdana;"> (BMTs) that can be used to predict body size were measured on each animal and values of BMTs measured were compared between both breeds. Very significant (P < 0.001) differences, in favour of White Fulani
文摘Cattle production is an important aspect of animal agriculture. Disease predisposition in cattle can lead to lowered productivity and poor animal welfare. To select and breed for the fittest cattle population, it is expedient that we understand the genetic basis of disease tolerance/resistance. Major histocompatibility complex (MHC) is a vital component of the immune system in vertebrates. Its genes are crucial determinants for immune response and resistance to infectious diseases. The bovine MHC is referred to as Bovine Lymphocyte antigen (BoLA) with its most functional and highly variable region located in the exon 2 (BoLA-DRB3.2). Over 100 alleles of BoLA-DRB3.2 have been identified in cattle and many studies have associated polymorphism in this region with disease resistance/susceptibility. In this study, we investigated the polymorphic nature of BoLA-DRB3.2 in the White Fulani and Muturu cattle breeds using a single PCR-sequence based typing. We identified 26 and 25 alleles in White Fulani and Muturu breeds, respectively, with only six alleles being mutual in the two breeds. Some of the alleles identified in this study have been noted as markers for disease status in cattle. BoLA-DRB3*014:01:01, BoLA-DRB3*011:01, and BoLA-DRB3*008:01 alleles have been associated with Bovine leukemia virus (BLV) resistance in cattle. BoLA-DRB3*014:01:01, BoLA-DRB3*001:01, and BoLA-DRB3*011:01 were linked with mastitis resistance in Japanese Holstein cows. While no inference can be drawn in terms of association with disease status, this study confirms the highly polymorphic and diverse nature of BoLA-DRB3 in White Fulani and Muturu cattle breeds.
