Bacterial community presumably plays an essential role in inhibiting pathogen colonization and maintaining the health of scallop larvae, but limiting data are available for Yesso scallop （Patinopecten yessoensisis Ja...Bacterial community presumably plays an essential role in inhibiting pathogen colonization and maintaining the health of scallop larvae, but limiting data are available for Yesso scallop （Patinopecten yessoensisis Jay, 1857） larval development stages. The aim of this study was to characterize and compare the bacterial communities associating with Yesso scallop larval development at fertilized egg S l, trochophora S2, D-shaped larvae S3, umbo larvae S4, and juvenile scallop S5 stages by Illumina high-throughput sequencing. Genomic DNA was extracted from the larvae and their associating baetera, and a gene segment covering V3-V4 region of 16S rRNA gene was amplified and sequenced using an Illumina Miseq sequencer. Overall, 106760 qualified sequences with an average length of 449 bp were obtained. Sequences were compared with those retrieved from 16S rRNA gene databases, and 4 phyla, 7 classes, 15 orders, 21 families, 31 genera were identified. Proteobacteria was predominant phylum, accounting for more than 99%, at all 5 larval development stages. At genus level, Pseudomonas was dominant at stages S1 （80.60%）, S2 （87.77%） and S5 （68.71%）, followed by Photobacterium （17.06%） and Aeromonas （1.64%） at stage S1, Serratia （6.94%）, Stenotrophomonas （3.08%） and Acinetobacter （1.2%） at stage S2, Shewanella （25.95%） and Pseudoalteromonas （4.57%） at stage S5. Moreover, genus Pseudoal- teromonas became dominant at stages S3 （44.85%） and S4 （56.02%）, followed by Photobacterium （29.82%）, Pseudomonas （11.86%）, Aliivibrio （8.60%） and Shewanella （3.39%） at stage S3, Pseudomonas （18.16%）, Aliivibrio （14.29%）, Shewanella （4.11%）, Psychro- monas （4.04%） and Psychrobacter （1.81%） at stage S4. From the results, we concluded that the bacterial community changed sig- nificantly at different development stages of Yesso Scallop larvae.展开更多
目的系统评价护士处方对2型糖尿病患者糖化血红蛋白(glycosylated hemoglobin,HbAlc)水平的影响。方法计算机检索PubMed、EMBASE、Web of Science、Cochrane Library、CINAHL数据库中有关护士处方对2型糖尿病患者HbAlc影响的随机对照试...目的系统评价护士处方对2型糖尿病患者糖化血红蛋白(glycosylated hemoglobin,HbAlc)水平的影响。方法计算机检索PubMed、EMBASE、Web of Science、Cochrane Library、CINAHL数据库中有关护士处方对2型糖尿病患者HbAlc影响的随机对照试验。对所得文献依据纳入与排除标准进行筛查,提取资料,评价文献质量,利用RevMan 5.3.0软件进行Meta分析。结果最终共纳入10篇文献,根据护士处方的形式不同,将纳入的文献进行Meta分析。Meta分析结果显示,在护士参与医生开处方的5项随机对照试验中,与护士不参与医生开处方对患者血糖的控制效果比较,差异无统计学意义[加权均数差(weight mean difference,WMD)=-0.34,95%可信区间(CI)(-0.71~0.02),P=0.07];在护士独立开处方的5项随机对照试验中,与医生开处方对患者血糖控制的效果比较,差异无统计学意义(WMD=-0.21,95%CI (-0.46~0.03),P=0.09)。结论在以医生为主导的医疗服务有限的情况下,授予护士独立处方权,可较好控制2型糖尿病患者的血糖水平。展开更多
基金financial support from Zhang Zidao Sland Group Co.,Ltd. for the project (99801214)
文摘Bacterial community presumably plays an essential role in inhibiting pathogen colonization and maintaining the health of scallop larvae, but limiting data are available for Yesso scallop （Patinopecten yessoensisis Jay, 1857） larval development stages. The aim of this study was to characterize and compare the bacterial communities associating with Yesso scallop larval development at fertilized egg S l, trochophora S2, D-shaped larvae S3, umbo larvae S4, and juvenile scallop S5 stages by Illumina high-throughput sequencing. Genomic DNA was extracted from the larvae and their associating baetera, and a gene segment covering V3-V4 region of 16S rRNA gene was amplified and sequenced using an Illumina Miseq sequencer. Overall, 106760 qualified sequences with an average length of 449 bp were obtained. Sequences were compared with those retrieved from 16S rRNA gene databases, and 4 phyla, 7 classes, 15 orders, 21 families, 31 genera were identified. Proteobacteria was predominant phylum, accounting for more than 99%, at all 5 larval development stages. At genus level, Pseudomonas was dominant at stages S1 （80.60%）, S2 （87.77%） and S5 （68.71%）, followed by Photobacterium （17.06%） and Aeromonas （1.64%） at stage S1, Serratia （6.94%）, Stenotrophomonas （3.08%） and Acinetobacter （1.2%） at stage S2, Shewanella （25.95%） and Pseudoalteromonas （4.57%） at stage S5. Moreover, genus Pseudoal- teromonas became dominant at stages S3 （44.85%） and S4 （56.02%）, followed by Photobacterium （29.82%）, Pseudomonas （11.86%）, Aliivibrio （8.60%） and Shewanella （3.39%） at stage S3, Pseudomonas （18.16%）, Aliivibrio （14.29%）, Shewanella （4.11%）, Psychro- monas （4.04%） and Psychrobacter （1.81%） at stage S4. From the results, we concluded that the bacterial community changed sig- nificantly at different development stages of Yesso Scallop larvae.
文摘目的系统评价护士处方对2型糖尿病患者糖化血红蛋白(glycosylated hemoglobin,HbAlc)水平的影响。方法计算机检索PubMed、EMBASE、Web of Science、Cochrane Library、CINAHL数据库中有关护士处方对2型糖尿病患者HbAlc影响的随机对照试验。对所得文献依据纳入与排除标准进行筛查,提取资料,评价文献质量,利用RevMan 5.3.0软件进行Meta分析。结果最终共纳入10篇文献,根据护士处方的形式不同,将纳入的文献进行Meta分析。Meta分析结果显示,在护士参与医生开处方的5项随机对照试验中,与护士不参与医生开处方对患者血糖的控制效果比较,差异无统计学意义[加权均数差(weight mean difference,WMD)=-0.34,95%可信区间(CI)(-0.71~0.02),P=0.07];在护士独立开处方的5项随机对照试验中,与医生开处方对患者血糖控制的效果比较,差异无统计学意义(WMD=-0.21,95%CI (-0.46~0.03),P=0.09)。结论在以医生为主导的医疗服务有限的情况下,授予护士独立处方权,可较好控制2型糖尿病患者的血糖水平。
