RAPD （randomly amplified polymorphic DNA） markers were employed to characterize polymorphisms among 5 provenances of Acacia leucophloea and to detect genetic relatedness of the species with 6 other acacias （A. holo...RAPD （randomly amplified polymorphic DNA） markers were employed to characterize polymorphisms among 5 provenances of Acacia leucophloea and to detect genetic relatedness of the species with 6 other acacias （A. holosericea, A. auriculiformis, A. mangium, A. dealbata, A. ferruginea, and A. nilotica） widely grown in India. Of 194 markers scored for the provenances, 29.38% exhibited polymorphism. Also, 326 markers were generated among 7 species of Acacia, accounting for 55.82% of the polymorphisms. The fifteen 10-mer primers employed were capable of producing 1-8 polymorphic bands for the provenances, and 6-17 for all seven species of Acacia. The genetic similarity coefficient based on Jaccard＇ s coefficient revealed that provenances Thirumangalam and Dharmapuri were closely related. The dendrogram based on a sequential agglomerative hierarchical non-overlapping （SAHN） clustering analysis grouped 4 provenances of A. leucophloea （Dharapuram, Thirumangalam, Pudukottai and Dharmapuri） into one cluster and the other provenance, Sendurai, into a separate cluster. The genetic similarity matrix for 7 Acacia species showed that A. nilotica and A. dealbata were distantly related, while A. holosericea and A. ferruginea were very closely related. Cluster analysis grouped the species of Acacias into 3 major groups of which A. dealbata alone formed a separate group. The RAPD markers generated 36 provenance-specific markers and 162 species-specific markers that could have strong applications for species identification and tree breeding programs for A. leucophloea and for other Acacia species included in this study.展开更多
Objective:To study diversity and distribution of tree hole mosquitoes at Puducherry Union Territory.Methods:Random collections were carried out in tree holes at collection sites by using suction tube.Mosquitoes are id...Objective:To study diversity and distribution of tree hole mosquitoes at Puducherry Union Territory.Methods:Random collections were carried out in tree holes at collection sites by using suction tube.Mosquitoes are identified by standard entomological procedures.Results:A total of 235 mosquitoes were collected from tree holes,comprising 3 genera and 12 species.They are,Aedes aegypti,Aedes albopictus,Aedes stokesi,Aedes simpsoni,Anopheles subpictus,Anopheles stephensi,Anopheles culiciformis,Anopheles maculatus,Culex quinquefasciatus,Culex pseudovishnui,Culex tritaeniorhynchus,and Culex decens.The results reveal thatAedes species is the dominant species in tree holes.Simpson’s dominance index and Shanon-Wiener diversity index of 0.1827 and 0.8336 were respectively recorded for all tree hole mosquitoes.Conclusions:The diversity studies of tree hole mosquitoes in the study area are necessary for the implementation of appropriate control strategies.展开更多
The E. tarda bacterial culture isolated from infected fish, was confirmed by morphological and biochemical characterization. GAPDH gene of 996 bp was amplified from bacterial genomic DNA. GAPDH gene was cloned in pTZ5...The E. tarda bacterial culture isolated from infected fish, was confirmed by morphological and biochemical characterization. GAPDH gene of 996 bp was amplified from bacterial genomic DNA. GAPDH gene was cloned in pTZ57R/T cloning vector. The positive clone was sequenced. The sequencing result showed very homology with published sequence from pathogenic E. tarda. The sequence (nucleotide and amino acid) divergence values were very low between E. tarda isolates from India (KF142190) and China (HQ697337-38) (Figure 4 and Figure 5). However, the divergence value was high when compared with E. tarda isolates from Japan (AB198939) and this value was higher than the inter-specific divergence value (E. tarda-E. ictaluri, E. tarda-E. coli & E. tarda-Klebsiella species).展开更多
基金supported by a grant from the Korea Institute of Planning and Evaluation for Technology in Food,Agriculture,Forestry and Fisheries(IPET)through the AgriBioindustry Technology Development Programfunded by the Ministry of Agriculture,Food and Rural Affairs(MAFRA)(No.314009-3)
文摘RAPD （randomly amplified polymorphic DNA） markers were employed to characterize polymorphisms among 5 provenances of Acacia leucophloea and to detect genetic relatedness of the species with 6 other acacias （A. holosericea, A. auriculiformis, A. mangium, A. dealbata, A. ferruginea, and A. nilotica） widely grown in India. Of 194 markers scored for the provenances, 29.38% exhibited polymorphism. Also, 326 markers were generated among 7 species of Acacia, accounting for 55.82% of the polymorphisms. The fifteen 10-mer primers employed were capable of producing 1-8 polymorphic bands for the provenances, and 6-17 for all seven species of Acacia. The genetic similarity coefficient based on Jaccard＇ s coefficient revealed that provenances Thirumangalam and Dharmapuri were closely related. The dendrogram based on a sequential agglomerative hierarchical non-overlapping （SAHN） clustering analysis grouped 4 provenances of A. leucophloea （Dharapuram, Thirumangalam, Pudukottai and Dharmapuri） into one cluster and the other provenance, Sendurai, into a separate cluster. The genetic similarity matrix for 7 Acacia species showed that A. nilotica and A. dealbata were distantly related, while A. holosericea and A. ferruginea were very closely related. Cluster analysis grouped the species of Acacias into 3 major groups of which A. dealbata alone formed a separate group. The RAPD markers generated 36 provenance-specific markers and 162 species-specific markers that could have strong applications for species identification and tree breeding programs for A. leucophloea and for other Acacia species included in this study.
基金Supported by University Grants Commission,New Delhi,India[UGC Ref.Letter No.42-558/2013(SR)].
文摘Objective:To study diversity and distribution of tree hole mosquitoes at Puducherry Union Territory.Methods:Random collections were carried out in tree holes at collection sites by using suction tube.Mosquitoes are identified by standard entomological procedures.Results:A total of 235 mosquitoes were collected from tree holes,comprising 3 genera and 12 species.They are,Aedes aegypti,Aedes albopictus,Aedes stokesi,Aedes simpsoni,Anopheles subpictus,Anopheles stephensi,Anopheles culiciformis,Anopheles maculatus,Culex quinquefasciatus,Culex pseudovishnui,Culex tritaeniorhynchus,and Culex decens.The results reveal thatAedes species is the dominant species in tree holes.Simpson’s dominance index and Shanon-Wiener diversity index of 0.1827 and 0.8336 were respectively recorded for all tree hole mosquitoes.Conclusions:The diversity studies of tree hole mosquitoes in the study area are necessary for the implementation of appropriate control strategies.
文摘The E. tarda bacterial culture isolated from infected fish, was confirmed by morphological and biochemical characterization. GAPDH gene of 996 bp was amplified from bacterial genomic DNA. GAPDH gene was cloned in pTZ57R/T cloning vector. The positive clone was sequenced. The sequencing result showed very homology with published sequence from pathogenic E. tarda. The sequence (nucleotide and amino acid) divergence values were very low between E. tarda isolates from India (KF142190) and China (HQ697337-38) (Figure 4 and Figure 5). However, the divergence value was high when compared with E. tarda isolates from Japan (AB198939) and this value was higher than the inter-specific divergence value (E. tarda-E. ictaluri, E. tarda-E. coli & E. tarda-Klebsiella species).
