In this Letter, we present B-scan-sectioned dynamic micro-optical coherence tomography(BD-MOCT) for high-quality subcellular dynamic contrast imaging. Dynamic micro-optical coherence tomography(D-MOCT) is a functional...In this Letter, we present B-scan-sectioned dynamic micro-optical coherence tomography(BD-MOCT) for high-quality subcellular dynamic contrast imaging. Dynamic micro-optical coherence tomography(D-MOCT) is a functional optical coherence tomography(OCT) technique performed on high-resolution(micron level) OCT systems;hundreds of consecutive B-scans need to be acquired for dynamic signal extraction, which requires relatively long data acquisition time. Bulk motions occurring during data acquisition(even at the micron level) may degrade the quality of the obtained dynamic contrast images. In BD-MOCT, each full B-scan is divided into several sub-B-scans, and each sub-B-scan repeats multiple times before the sample beam moves to the next sub-B-scan. After all of the sub-B-scans for a full B-scan are completely acquired, we stitch all of the sub-B-scans into the same number of full B-scans. In this way, the time interval between two consecutive stitched B-scans could be reduced multiple times for bulk-motion suppression. The performed scanning protocol modulates the scanning sequences of fast scanning and repeat scanning for improving the dynamic contrast image quality, while the total data acquisition time remains almost the same.展开更多
蒸馏米酒在蒸馏过程中会产生大量的蒸馏残液,而蒸馏残液具有巨大的经济潜力。该研究从蒸馏米酒的蒸馏残液中分离纯化出一种分子质量为8554 Da的杂多糖并命名为CRWPs,CRWPs主要由葡萄糖、半乳糖、甘露糖、葡萄糖醛酸、半乳糖醛酸、岩藻...蒸馏米酒在蒸馏过程中会产生大量的蒸馏残液,而蒸馏残液具有巨大的经济潜力。该研究从蒸馏米酒的蒸馏残液中分离纯化出一种分子质量为8554 Da的杂多糖并命名为CRWPs,CRWPs主要由葡萄糖、半乳糖、甘露糖、葡萄糖醛酸、半乳糖醛酸、岩藻糖和阿拉伯糖组成,质量占比分别为91.56%、2.13%、1.61%、1.84%、1.34%、0.67%和0.12%。流变学分析表明CRWPs溶液表现出典型的剪切变稀行为,同时具有假塑性流体的特征。采用原子力显微镜(atomic force microscope,AFM)观察CRWPs在溶液-云母片表面的表观形貌,发现多糖链间聚合堆积而形成点状或棒状结构,推测该多糖具有多分支的结构。通过2株双歧杆菌和2株乳杆菌的静态发酵对CRWPs的益生功能进行评价。结果表明,与空白组比,CRWPs能够显著促进这4株菌的生长和短链脂肪酸的产生,其中短双歧杆菌的总酸产量从(105.62±2.70)mmol/mL提升到(145.55±5.36)mmol/mL,表明CRWPs具有一定的益生活性。该研究对从蒸馏残液中提取的多糖CRWPs的益生元潜力进行了报道,为其在环境经济型食品行业中的潜在应用奠定了基础。展开更多
基金This work was supported by the Natural Science Foundation of Jiangsu Province(No.BK20210227).
文摘In this Letter, we present B-scan-sectioned dynamic micro-optical coherence tomography(BD-MOCT) for high-quality subcellular dynamic contrast imaging. Dynamic micro-optical coherence tomography(D-MOCT) is a functional optical coherence tomography(OCT) technique performed on high-resolution(micron level) OCT systems;hundreds of consecutive B-scans need to be acquired for dynamic signal extraction, which requires relatively long data acquisition time. Bulk motions occurring during data acquisition(even at the micron level) may degrade the quality of the obtained dynamic contrast images. In BD-MOCT, each full B-scan is divided into several sub-B-scans, and each sub-B-scan repeats multiple times before the sample beam moves to the next sub-B-scan. After all of the sub-B-scans for a full B-scan are completely acquired, we stitch all of the sub-B-scans into the same number of full B-scans. In this way, the time interval between two consecutive stitched B-scans could be reduced multiple times for bulk-motion suppression. The performed scanning protocol modulates the scanning sequences of fast scanning and repeat scanning for improving the dynamic contrast image quality, while the total data acquisition time remains almost the same.
文摘蒸馏米酒在蒸馏过程中会产生大量的蒸馏残液,而蒸馏残液具有巨大的经济潜力。该研究从蒸馏米酒的蒸馏残液中分离纯化出一种分子质量为8554 Da的杂多糖并命名为CRWPs,CRWPs主要由葡萄糖、半乳糖、甘露糖、葡萄糖醛酸、半乳糖醛酸、岩藻糖和阿拉伯糖组成,质量占比分别为91.56%、2.13%、1.61%、1.84%、1.34%、0.67%和0.12%。流变学分析表明CRWPs溶液表现出典型的剪切变稀行为,同时具有假塑性流体的特征。采用原子力显微镜(atomic force microscope,AFM)观察CRWPs在溶液-云母片表面的表观形貌,发现多糖链间聚合堆积而形成点状或棒状结构,推测该多糖具有多分支的结构。通过2株双歧杆菌和2株乳杆菌的静态发酵对CRWPs的益生功能进行评价。结果表明,与空白组比,CRWPs能够显著促进这4株菌的生长和短链脂肪酸的产生,其中短双歧杆菌的总酸产量从(105.62±2.70)mmol/mL提升到(145.55±5.36)mmol/mL,表明CRWPs具有一定的益生活性。该研究对从蒸馏残液中提取的多糖CRWPs的益生元潜力进行了报道,为其在环境经济型食品行业中的潜在应用奠定了基础。
