Phytic acid is the principal storage form of phosphorus in plant seeds and an essential signalling molecule in several regulatory processes of plant development.However,it is known as an anti-nutrient compound owing t...Phytic acid is the principal storage form of phosphorus in plant seeds and an essential signalling molecule in several regulatory processes of plant development.However,it is known as an anti-nutrient compound owing to its potent chelating property.Thus,reducing the phytic acid content in crops is desirable.Studies involving regulation of MIPS and IPK1 genes to generate low phytate rice have been reported earlier.However,the functional significance of OsITPK and the effect of its down-regulation on phytic acid content and the associated pleiotropic effects on rice have not yet been investigated.In this study,tissue specific RNA interference(RNAi)-mediated down-regulation of a major ITPK homolog(OsITP5/6K-1)resulted in 46.2%decrease in phytic acid content of T2 transgenic seeds with a subsequent 3-fold enhancement in the inorganic phosphorus content.Silencing of OsITP5/6K-1 altered the transcript levels of essential phytic acid pathway genes,without significantly affecting the transcript levels of other OsITPK homologs.Furthermore,the mapping of elements through X-ray microfluorescence analysis revealed significant changes in the spatial distribution pattern and translocation of elements in low phytate seeds.Additionally,low phytate polished seeds exhibited 1.3-fold and 1.6-fold enhancement in iron and zinc content in the grain endosperm,respectively.Silencing of OsITP5/6K-1 also altered the amino acid and myo-inositol content of the transgenic seeds.Our results successfully established that RNAi-mediated silencing of OsITP5/6K-1 gene significantly reduced the phytate levels in seeds without hampering the germination potential of seeds and plant growth.The present study provided an insight into the mechanism of phytic acid biosynthesis pathway.展开更多
Background： An oviduct-specific glycoprotein, OVGP1, is synthesized and secreted by non-ciliated epithelial cells of the mammalian oviduct which provides an essential milieu for reproductive functions. The present st...Background： An oviduct-specific glycoprotein, OVGP1, is synthesized and secreted by non-ciliated epithelial cells of the mammalian oviduct which provides an essential milieu for reproductive functions. The present study reports the effects of recombinant buffalo OVGP1 that lacks post-translational modifications, and native Buffalo OVGP1 isolated from oviductal tissue, on frozen-thawed sperm functions and in vitro embryo development.Results： The proportion of viable sperms was greater（P 〈 0.05） in the recombinant OVGP1-treated group compared to the native OVGP1-treated group at 2 h, 3 h, and 4 h of incubation. The proportion of motile sperms at3 h and 4 h of incubation; and membrane-intact sperms at 4 h was greater（P 〈 0.05） in the native OVGP1-treated group compared to the control and recombinant OVGP1-treated groups. The proportion of capacitated and acrosome-reacted sperms was greater（P 〈 0.05） in the native OVGP1-treated group compared to the recombinant OVGP1 group at 4 h. The rates of cleavage of embryos and their development to the blastocyst stage were greater（P 〈 0.05） in the presence of either native or recombinant OVGP1 in comparison to control at 10 μg/mL concentration as compared to 5 or 20 μg/mL.Conclusions： The study suggests that both native and recombinant OVGP1 impart a positive effect on various sperm features and in vitro embryo development. However, native OVGP1 was found to have a more pronounced effect in comparison to recombinant non-glycosylated OVGP1 on various sperm functions except viability. Hence,our current findings infer that glycosylation of OVGP1 might be essential in sustaining the sperm functions but not the in vitro embryo development.展开更多
The behaviour of marine risers is investigated under the forces produced by selfweight, wave and current and quasistatic motion of the top vessel. The parameters include the applied tension, geometric nonlinearity, ma...The behaviour of marine risers is investigated under the forces produced by selfweight, wave and current and quasistatic motion of the top vessel. The parameters include the applied tension, geometric nonlinearity, magnitude of surge, heave and pitch motion of the top vessel and wave height. The study shows that the above parameters have considerable influence on the riser response.展开更多
The regulatory functions of soil are getting attention among the scientists and Soil Organic Carbon (SOC) is an important indicator of soil health. The impact of differential use of land on SOC and other edaphic prope...The regulatory functions of soil are getting attention among the scientists and Soil Organic Carbon (SOC) is an important indicator of soil health. The impact of differential use of land on SOC and other edaphic properties were analysed in three important Land use land cover (LULC) system of Tripura, northeast India. Soil cores were divided into four depth profiles (0 - 10, 10 - 30, 30 - 50 and 50 - 100 cm) to observe the changes of edaphic properties into the soil depth gradient. Our results suggest that SOC in the top profile of Managed Plantation (MP) and Jhum Fellow (JF) was 51.68% and 48.55%, less than Natural Forest (NF). From 0 - 10 to 10 - 30 cm soil depth, 43.3%, 8.4% and 39.4% decrease in NF MP and JF found. Total stock of SOC (Mg C·ha<sup>﹣</sup><sup>1</sup>) was found highest in JF (121.87), followed by NF (117.12) and MP (85.34). In top profile, conversion of NF into MP and NF into JF led to 39% and 11% decrease in SOC. The significant variation in SOC stock was found among different LULC under this study (F<sub>2,12</sub> = 16.94, P ≤ 0.001). In 0 - 10 cm soil depth, maximum value of bulk density (gm·cm<sup>﹣</sup><sup>3</sup>) was found in MP (1.39) followed by JF (1.27) and NF (1.23). In top profile, significant variation was found among LULC (p < 0.001). Soil pH and moisture content significantly varied (p < 0.05) in 0 - 10, 10 - 30 and 50 - 100 cm soil depth. In contrast to that significant change in soil temperature was found at 30 - 50 cm (p < 0.001) and 50 - 100 cm (p < 0.001). It was observed that JF had highest overall SOC stock than NF and MP. Conversion of NF into MP and JF results significant loss of SOC at 0 - 10 and 10 - 30 cm depth profile. We also found that NF conversion had significant impact on the change in the soil C pool.展开更多
The types,frequency,and intensity of human interference with forests markedly affect the vegetation dynamics.Assessment of the impact of anthropogenic disturbances on forest diversity and structure is one of the centr...The types,frequency,and intensity of human interference with forests markedly affect the vegetation dynamics.Assessment of the impact of anthropogenic disturbances on forest diversity and structure is one of the central issues related to human behaviour and forest ecosystems and management.Both species diversity and density are very sensitive to local anthropogenic disturbance,which can be reduced depending on land-use systems.We used 10 line transects of 500 x 10 m to record and measure all plants >10 cm girth in some of the remaining forests surrounding villages in Tripura,Northeast India.To predict the effects of anthropogenic pressure on species richness and forest structure,we recorded and scored all ongoing disturbances in all transects of the forests.Although our study did not show any significant effects on the mean number of species,all diversity indices were significantly affected by disturbance.Total stem density(F = 128.60,p < 0.0001) and basal area(F = 65.30,p < 0.0001) of the forest stands were significantly affected by disturbance.Disturbance significantly(F= 84.81,p < 0.0001) impacts the abundance of mature voluminous trees removed by extensive illegal logging.Further,stem density at the middle(F= 10.01,p < 0.05) and upper(F= 131.70,p < 0.0001) canopy stories was also reduced by high disturbance intensity.The present analysis will be useful to policy-makers and planners for implementation of sustainable forest management at both the local and regional scale.展开更多
The present study was undertaken to measure a-b, a-d and td ridge counts of deaf patients and to compare the dermatoglyphic differences among deaf individuals. The data consists of 208 heredo-familial Punjabi Khatri a...The present study was undertaken to measure a-b, a-d and td ridge counts of deaf patients and to compare the dermatoglyphic differences among deaf individuals. The data consists of 208 heredo-familial Punjabi Khatri and Arora deaf subjects, including 118 males and 90 femeles. The control sample consists of 228 Punjabis, comprising of 128 males and 100 females. The present study indicates lower incidence of ’a-b’, ’a-d’ and ’td’ ridge counts in deaf groups when compared to the corresponding normal groups.展开更多
文摘Phytic acid is the principal storage form of phosphorus in plant seeds and an essential signalling molecule in several regulatory processes of plant development.However,it is known as an anti-nutrient compound owing to its potent chelating property.Thus,reducing the phytic acid content in crops is desirable.Studies involving regulation of MIPS and IPK1 genes to generate low phytate rice have been reported earlier.However,the functional significance of OsITPK and the effect of its down-regulation on phytic acid content and the associated pleiotropic effects on rice have not yet been investigated.In this study,tissue specific RNA interference(RNAi)-mediated down-regulation of a major ITPK homolog(OsITP5/6K-1)resulted in 46.2%decrease in phytic acid content of T2 transgenic seeds with a subsequent 3-fold enhancement in the inorganic phosphorus content.Silencing of OsITP5/6K-1 altered the transcript levels of essential phytic acid pathway genes,without significantly affecting the transcript levels of other OsITPK homologs.Furthermore,the mapping of elements through X-ray microfluorescence analysis revealed significant changes in the spatial distribution pattern and translocation of elements in low phytate seeds.Additionally,low phytate polished seeds exhibited 1.3-fold and 1.6-fold enhancement in iron and zinc content in the grain endosperm,respectively.Silencing of OsITP5/6K-1 also altered the amino acid and myo-inositol content of the transgenic seeds.Our results successfully established that RNAi-mediated silencing of OsITP5/6K-1 gene significantly reduced the phytate levels in seeds without hampering the germination potential of seeds and plant growth.The present study provided an insight into the mechanism of phytic acid biosynthesis pathway.
基金funded by "Niche area of excellence,Indian Council of Agricultural Research(ICAR),India"
文摘Background： An oviduct-specific glycoprotein, OVGP1, is synthesized and secreted by non-ciliated epithelial cells of the mammalian oviduct which provides an essential milieu for reproductive functions. The present study reports the effects of recombinant buffalo OVGP1 that lacks post-translational modifications, and native Buffalo OVGP1 isolated from oviductal tissue, on frozen-thawed sperm functions and in vitro embryo development.Results： The proportion of viable sperms was greater（P 〈 0.05） in the recombinant OVGP1-treated group compared to the native OVGP1-treated group at 2 h, 3 h, and 4 h of incubation. The proportion of motile sperms at3 h and 4 h of incubation; and membrane-intact sperms at 4 h was greater（P 〈 0.05） in the native OVGP1-treated group compared to the control and recombinant OVGP1-treated groups. The proportion of capacitated and acrosome-reacted sperms was greater（P 〈 0.05） in the native OVGP1-treated group compared to the recombinant OVGP1 group at 4 h. The rates of cleavage of embryos and their development to the blastocyst stage were greater（P 〈 0.05） in the presence of either native or recombinant OVGP1 in comparison to control at 10 μg/mL concentration as compared to 5 or 20 μg/mL.Conclusions： The study suggests that both native and recombinant OVGP1 impart a positive effect on various sperm features and in vitro embryo development. However, native OVGP1 was found to have a more pronounced effect in comparison to recombinant non-glycosylated OVGP1 on various sperm functions except viability. Hence,our current findings infer that glycosylation of OVGP1 might be essential in sustaining the sperm functions but not the in vitro embryo development.
文摘The behaviour of marine risers is investigated under the forces produced by selfweight, wave and current and quasistatic motion of the top vessel. The parameters include the applied tension, geometric nonlinearity, magnitude of surge, heave and pitch motion of the top vessel and wave height. The study shows that the above parameters have considerable influence on the riser response.
文摘The regulatory functions of soil are getting attention among the scientists and Soil Organic Carbon (SOC) is an important indicator of soil health. The impact of differential use of land on SOC and other edaphic properties were analysed in three important Land use land cover (LULC) system of Tripura, northeast India. Soil cores were divided into four depth profiles (0 - 10, 10 - 30, 30 - 50 and 50 - 100 cm) to observe the changes of edaphic properties into the soil depth gradient. Our results suggest that SOC in the top profile of Managed Plantation (MP) and Jhum Fellow (JF) was 51.68% and 48.55%, less than Natural Forest (NF). From 0 - 10 to 10 - 30 cm soil depth, 43.3%, 8.4% and 39.4% decrease in NF MP and JF found. Total stock of SOC (Mg C·ha<sup>﹣</sup><sup>1</sup>) was found highest in JF (121.87), followed by NF (117.12) and MP (85.34). In top profile, conversion of NF into MP and NF into JF led to 39% and 11% decrease in SOC. The significant variation in SOC stock was found among different LULC under this study (F<sub>2,12</sub> = 16.94, P ≤ 0.001). In 0 - 10 cm soil depth, maximum value of bulk density (gm·cm<sup>﹣</sup><sup>3</sup>) was found in MP (1.39) followed by JF (1.27) and NF (1.23). In top profile, significant variation was found among LULC (p < 0.001). Soil pH and moisture content significantly varied (p < 0.05) in 0 - 10, 10 - 30 and 50 - 100 cm soil depth. In contrast to that significant change in soil temperature was found at 30 - 50 cm (p < 0.001) and 50 - 100 cm (p < 0.001). It was observed that JF had highest overall SOC stock than NF and MP. Conversion of NF into MP and JF results significant loss of SOC at 0 - 10 and 10 - 30 cm depth profile. We also found that NF conversion had significant impact on the change in the soil C pool.
文摘The types,frequency,and intensity of human interference with forests markedly affect the vegetation dynamics.Assessment of the impact of anthropogenic disturbances on forest diversity and structure is one of the central issues related to human behaviour and forest ecosystems and management.Both species diversity and density are very sensitive to local anthropogenic disturbance,which can be reduced depending on land-use systems.We used 10 line transects of 500 x 10 m to record and measure all plants >10 cm girth in some of the remaining forests surrounding villages in Tripura,Northeast India.To predict the effects of anthropogenic pressure on species richness and forest structure,we recorded and scored all ongoing disturbances in all transects of the forests.Although our study did not show any significant effects on the mean number of species,all diversity indices were significantly affected by disturbance.Total stem density(F = 128.60,p < 0.0001) and basal area(F = 65.30,p < 0.0001) of the forest stands were significantly affected by disturbance.Disturbance significantly(F= 84.81,p < 0.0001) impacts the abundance of mature voluminous trees removed by extensive illegal logging.Further,stem density at the middle(F= 10.01,p < 0.05) and upper(F= 131.70,p < 0.0001) canopy stories was also reduced by high disturbance intensity.The present analysis will be useful to policy-makers and planners for implementation of sustainable forest management at both the local and regional scale.
文摘The present study was undertaken to measure a-b, a-d and td ridge counts of deaf patients and to compare the dermatoglyphic differences among deaf individuals. The data consists of 208 heredo-familial Punjabi Khatri and Arora deaf subjects, including 118 males and 90 femeles. The control sample consists of 228 Punjabis, comprising of 128 males and 100 females. The present study indicates lower incidence of ’a-b’, ’a-d’ and ’td’ ridge counts in deaf groups when compared to the corresponding normal groups.
